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Protein

Plasma membrane calcium-transporting ATPase 1

Gene

ATP2B1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4754-aspartylphosphate intermediate1
Metal bindingi797MagnesiumBy similarity1
Metal bindingi801MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS01020-MONOMER.
BRENDAi3.6.3.8. 2681.
ReactomeiR-HSA-418359. Reduction of cytosolic Ca++ levels.
R-HSA-5578775. Ion homeostasis.
R-HSA-936837. Ion transport by P-type ATPases.

Protein family/group databases

TCDBi3.A.3.2.25. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma membrane calcium-transporting ATPase 1 (EC:3.6.3.8)
Short name:
PMCA1
Alternative name(s):
Plasma membrane calcium ATPase isoform 1
Plasma membrane calcium pump isoform 1
Gene namesi
Name:ATP2B1
Synonyms:PMCA1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:814. ATP2B1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 97CytoplasmicSequence analysisAdd BLAST96
Transmembranei98 – 118HelicalSequence analysisAdd BLAST21
Topological domaini119 – 154ExtracellularSequence analysisAdd BLAST36
Transmembranei155 – 175HelicalSequence analysisAdd BLAST21
Topological domaini176 – 366CytoplasmicSequence analysisAdd BLAST191
Transmembranei367 – 386HelicalSequence analysisAdd BLAST20
Topological domaini387 – 419ExtracellularSequence analysisAdd BLAST33
Transmembranei420 – 437HelicalSequence analysisAdd BLAST18
Topological domaini438 – 852CytoplasmicSequence analysisAdd BLAST415
Transmembranei853 – 872HelicalSequence analysisAdd BLAST20
Topological domaini873 – 882ExtracellularSequence analysis10
Transmembranei883 – 903HelicalSequence analysisAdd BLAST21
Topological domaini904 – 923CytoplasmicSequence analysisAdd BLAST20
Transmembranei924 – 946HelicalSequence analysisAdd BLAST23
Topological domaini947 – 964ExtracellularSequence analysisAdd BLAST18
Transmembranei965 – 986HelicalSequence analysisAdd BLAST22
Topological domaini987 – 1005CytoplasmicSequence analysisAdd BLAST19
Transmembranei1006 – 1027HelicalSequence analysisAdd BLAST22
Topological domaini1028 – 1037ExtracellularSequence analysis10
Transmembranei1038 – 1059HelicalSequence analysisAdd BLAST22
Topological domaini1060 – 1258CytoplasmicSequence analysisAdd BLAST199

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi490.
OpenTargetsiENSG00000070961.
PharmGKBiPA25107.

Polymorphism and mutation databases

BioMutaiATP2B1.
DMDMi14286104.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000462092 – 1258Plasma membrane calcium-transporting ATPase 1Add BLAST1257

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycineCombined sources1
Modified residuei8PhosphoserineCombined sources1
Modified residuei17PhosphoserineCombined sources1
Modified residuei338PhosphoserineBy similarity1
Modified residuei1116Phosphothreonine; by PKC1 Publication1
Modified residuei1178PhosphoserineBy similarity1
Modified residuei1193PhosphoserineCombined sources1
Modified residuei1203PhosphothreonineCombined sources1
Modified residuei1216Phosphoserine; by PKACombined sources1
Modified residuei1220PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP20020.
MaxQBiP20020.
PaxDbiP20020.
PeptideAtlasiP20020.
PRIDEiP20020.

PTM databases

iPTMnetiP20020.
PhosphoSitePlusiP20020.
SwissPalmiP20020.

Expressioni

Tissue specificityi

Isoform B is ubiquitously expressed. Isoform C is found in brain cortex, skeletal muscle and heart muscle. Isoform D has only been found in fetal skeletal muscle. Isoform K has been found in small intestine and liver.

Gene expression databases

BgeeiENSG00000070961.
CleanExiHS_ATP2B1.
ExpressionAtlasiP20020. baseline and differential.
GenevisibleiP20020. HS.

Organism-specific databases

HPAiCAB005605.
HPA011166.
HPA012945.

Interactioni

Subunit structurei

Interacts with PDZD11. Interacts with SLC35G1 and STIM1.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi106980. 36 interactors.
IntActiP20020. 24 interactors.
MINTiMINT-5004114.
STRINGi9606.ENSP00000261173.

Structurei

3D structure databases

ProteinModelPortaliP20020.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1100 – 1117Calmodulin-binding subdomain A1 PublicationAdd BLAST18
Regioni1118 – 1127Calmodulin-binding subdomain B1 Publication10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi296 – 299Poly-Glu4

Domaini

The calmodulin-binding subdomain B is different in the different splice variants and shows pH dependent calmodulin binding properties in isoforms A, C, D and E.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
GeneTreeiENSGT00510000046331.
HOVERGENiHBG061286.
InParanoidiP20020.
KOiK05850.
OMAiIWELAWI.
PhylomeDBiP20020.
TreeFamiTF300330.

Family and domain databases

Gene3Di1.20.1110.10. 1 hit.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR030319. ATP2B1/4.
IPR022141. ATP_Ca_trans_C.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF276. PTHR24093:SF276. 3 hits.
PfamiPF12424. ATP_Ca_trans_C. 2 hits.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 2 hits.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform D (identifier: P20020-1) [UniParc]FASTAAdd to basket
Also known as: CIV, hPMCA1d

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDMANNSVA YSGVKNSLKE ANHDGDFGIT LAELRALMEL RSTDALRKIQ
60 70 80 90 100
ESYGDVYGIC TKLKTSPNEG LSGNPADLER REAVFGKNFI PPKKPKTFLQ
110 120 130 140 150
LVWEALQDVT LIILEIAAIV SLGLSFYQPP EGDNALCGEV SVGEEEGEGE
160 170 180 190 200
TGWIEGAAIL LSVVCVVLVT AFNDWSKEKQ FRGLQSRIEQ EQKFTVIRGG
210 220 230 240 250
QVIQIPVADI TVGDIAQVKY GDLLPADGIL IQGNDLKIDE SSLTGESDHV
260 270 280 290 300
KKSLDKDPLL LSGTHVMEGS GRMVVTAVGV NSQTGIIFTL LGAGGEEEEK
310 320 330 340 350
KDEKKKEKKN KKQDGAIENR NKAKAQDGAA MEMQPLKSEE GGDGDEKDKK
360 370 380 390 400
KANLPKKEKS VLQGKLTKLA VQIGKAGLLM SAITVIILVL YFVIDTFWVQ
410 420 430 440 450
KRPWLAECTP IYIQYFVKFF IIGVTVLVVA VPEGLPLAVT ISLAYSVKKM
460 470 480 490 500
MKDNNLVRHL DACETMGNAT AICSDKTGTL TMNRMTVVQA YINEKHYKKV
510 520 530 540 550
PEPEAIPPNI LSYLVTGISV NCAYTSKILP PEKEGGLPRH VGNKTECALL
560 570 580 590 600
GLLLDLKRDY QDVRNEIPEE ALYKVYTFNS VRKSMSTVLK NSDGSYRIFS
610 620 630 640 650
KGASEIILKK CFKILSANGE AKVFRPRDRD DIVKTVIEPM ASEGLRTICL
660 670 680 690 700
AFRDFPAGEP EPEWDNENDI VTGLTCIAVV GIEDPVRPEV PDAIKKCQRA
710 720 730 740 750
GITVRMVTGD NINTARAIAT KCGILHPGED FLCLEGKDFN RRIRNEKGEI
760 770 780 790 800
EQERIDKIWP KLRVLARSSP TDKHTLVKGI IDSTVSDQRQ VVAVTGDGTN
810 820 830 840 850
DGPALKKADV GFAMGIAGTD VAKEASDIIL TDDNFTSIVK AVMWGRNVYD
860 870 880 890 900
SISKFLQFQL TVNVVAVIVA FTGACITQDS PLKAVQMLWV NLIMDTLASL
910 920 930 940 950
ALATEPPTES LLLRKPYGRN KPLISRTMMK NILGHAFYQL VVVFTLLFAG
960 970 980 990 1000
EKFFDIDSGR NAPLHAPPSE HYTIVFNTFV LMQLFNEINA RKIHGERNVF
1010 1020 1030 1040 1050
EGIFNNAIFC TIVLGTFVVQ IIIVQFGGKP FSCSELSIEQ WLWSIFLGMG
1060 1070 1080 1090 1100
TLLWGQLIST IPTSRLKFLK EAGHGTQKEE IPEEELAEDV EEIDHAEREL
1110 1120 1130 1140 1150
RRGQILWFRG LNRIQTQMDV VNAFQSGSSI QGALRRQPSI ASQHHDVTNI
1160 1170 1180 1190 1200
STPTHIRVVN AFRSSLYEGL EKPESRSSIH NFMTHPEFRI EDSEPHIPLI
1210 1220 1230 1240 1250
DDTDAEDDAP TKRNSSPPPS PNKNNNAVDS GIHLTIEMNK SATSSSPGSP

LHSLETSL
Length:1,258
Mass (Da):138,755
Last modified:June 1, 2001 - v3
Checksum:i7037112747FC9B0A
GO
Isoform A (identifier: P20020-2) [UniParc]FASTAAdd to basket
Also known as: CII, hPMCA1a

The sequence of this isoform differs from the canonical sequence as follows:
     1156-1258: IRVVNAFRSS...SPLHSLETSL → VVFSSSTASTTVGYSSGECIS

Show »
Length:1,176
Mass (Da):129,516
Checksum:i5672202C16491703
GO
Isoform B (identifier: P20020-3) [UniParc]FASTAAdd to basket
Also known as: CI, hPMCA1b

The sequence of this isoform differs from the canonical sequence as follows:
     1118-1155: Missing.

Show »
Length:1,220
Mass (Da):134,685
Checksum:i7E75C19B1A501423
GO
Isoform C (identifier: P20020-4) [UniParc]FASTAAdd to basket
Also known as: CIII, hPMCA1c

The sequence of this isoform differs from the canonical sequence as follows:
     1147-1155: Missing.

Show »
Length:1,249
Mass (Da):137,804
Checksum:i8F56F132BE096587
GO
Isoform E (identifier: P20020-5) [UniParc]FASTAAdd to basket
Also known as: CV

The sequence of this isoform differs from the canonical sequence as follows:
     1156-1258: IRVVNAFRSS...SPLHSLETSL → VVFSSSTASTTVGFEW

Show »
Length:1,171
Mass (Da):129,152
Checksum:i9FC9B1AA3B32B384
GO
Isoform K (identifier: P20020-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1021-1056: Missing.
     1118-1155: Missing.

Show »
Length:1,184
Mass (Da):130,617
Checksum:iB1FB3E7116D2FDA2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti259 – 262LLLS → MSAT in AAA36000 (PubMed:8386431).Curated4

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_000698267M → R Rare polymorphism. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0003721021 – 1056Missing in isoform K. CuratedAdd BLAST36
Alternative sequenceiVSP_0003731118 – 1155Missing in isoform B and isoform K. 2 PublicationsAdd BLAST38
Alternative sequenceiVSP_0003741147 – 1155Missing in isoform C. Curated9
Alternative sequenceiVSP_0003751156 – 1258IRVVN…LETSL → VVFSSSTASTTVGYSSGECI S in isoform A. CuratedAdd BLAST103
Alternative sequenceiVSP_0003761156 – 1258IRVVN…LETSL → VVFSSSTASTTVGFEW in isoform E. CuratedAdd BLAST103

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04027 mRNA. Translation: AAA74511.1.
M95541 mRNA. Translation: AAA35999.1.
M95542 mRNA. Translation: AAA36000.1.
L14561 Genomic DNA. Translation: AAD09924.1.
L14561 Genomic DNA. Translation: AAD09925.1.
M25824 Genomic DNA. Translation: AAA58383.1.
M25824 Genomic DNA. Translation: AAA58382.1.
M25824 Genomic DNA. Translation: AAA58381.1.
S49852 mRNA. Translation: AAB24324.1.
U15686 mRNA. Translation: AAA60983.1.
U15687 mRNA. Translation: AAA60984.1.
CCDSiCCDS41817.1. [P20020-2]
CCDS9035.1. [P20020-3]
PIRiA30802.
E49570.
I55491.
I70165.
RefSeqiNP_001001323.1. NM_001001323.1. [P20020-2]
NP_001673.2. NM_001682.2. [P20020-3]
XP_011536709.1. XM_011538407.2. [P20020-4]
XP_016874847.1. XM_017019358.1. [P20020-6]
UniGeneiHs.506276.

Genome annotation databases

EnsembliENST00000261173; ENSP00000261173; ENSG00000070961. [P20020-3]
ENST00000359142; ENSP00000352054; ENSG00000070961. [P20020-2]
ENST00000428670; ENSP00000392043; ENSG00000070961. [P20020-3]
GeneIDi490.
KEGGihsa:490.
UCSCiuc001tbg.4. human. [P20020-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04027 mRNA. Translation: AAA74511.1.
M95541 mRNA. Translation: AAA35999.1.
M95542 mRNA. Translation: AAA36000.1.
L14561 Genomic DNA. Translation: AAD09924.1.
L14561 Genomic DNA. Translation: AAD09925.1.
M25824 Genomic DNA. Translation: AAA58383.1.
M25824 Genomic DNA. Translation: AAA58382.1.
M25824 Genomic DNA. Translation: AAA58381.1.
S49852 mRNA. Translation: AAB24324.1.
U15686 mRNA. Translation: AAA60983.1.
U15687 mRNA. Translation: AAA60984.1.
CCDSiCCDS41817.1. [P20020-2]
CCDS9035.1. [P20020-3]
PIRiA30802.
E49570.
I55491.
I70165.
RefSeqiNP_001001323.1. NM_001001323.1. [P20020-2]
NP_001673.2. NM_001682.2. [P20020-3]
XP_011536709.1. XM_011538407.2. [P20020-4]
XP_016874847.1. XM_017019358.1. [P20020-6]
UniGeneiHs.506276.

3D structure databases

ProteinModelPortaliP20020.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106980. 36 interactors.
IntActiP20020. 24 interactors.
MINTiMINT-5004114.
STRINGi9606.ENSP00000261173.

Protein family/group databases

TCDBi3.A.3.2.25. the p-type atpase (p-atpase) superfamily.

PTM databases

iPTMnetiP20020.
PhosphoSitePlusiP20020.
SwissPalmiP20020.

Polymorphism and mutation databases

BioMutaiATP2B1.
DMDMi14286104.

Proteomic databases

EPDiP20020.
MaxQBiP20020.
PaxDbiP20020.
PeptideAtlasiP20020.
PRIDEiP20020.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261173; ENSP00000261173; ENSG00000070961. [P20020-3]
ENST00000359142; ENSP00000352054; ENSG00000070961. [P20020-2]
ENST00000428670; ENSP00000392043; ENSG00000070961. [P20020-3]
GeneIDi490.
KEGGihsa:490.
UCSCiuc001tbg.4. human. [P20020-1]

Organism-specific databases

CTDi490.
DisGeNETi490.
GeneCardsiATP2B1.
HGNCiHGNC:814. ATP2B1.
HPAiCAB005605.
HPA011166.
HPA012945.
MIMi108731. gene.
neXtProtiNX_P20020.
OpenTargetsiENSG00000070961.
PharmGKBiPA25107.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
GeneTreeiENSGT00510000046331.
HOVERGENiHBG061286.
InParanoidiP20020.
KOiK05850.
OMAiIWELAWI.
PhylomeDBiP20020.
TreeFamiTF300330.

Enzyme and pathway databases

BioCyciZFISH:HS01020-MONOMER.
BRENDAi3.6.3.8. 2681.
ReactomeiR-HSA-418359. Reduction of cytosolic Ca++ levels.
R-HSA-5578775. Ion homeostasis.
R-HSA-936837. Ion transport by P-type ATPases.

Miscellaneous databases

ChiTaRSiATP2B1. human.
GeneWikiiATP2B1.
GenomeRNAii490.
PROiP20020.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000070961.
CleanExiHS_ATP2B1.
ExpressionAtlasiP20020. baseline and differential.
GenevisibleiP20020. HS.

Family and domain databases

Gene3Di1.20.1110.10. 1 hit.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR030319. ATP2B1/4.
IPR022141. ATP_Ca_trans_C.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF276. PTHR24093:SF276. 3 hits.
PfamiPF12424. ATP_Ca_trans_C. 2 hits.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 2 hits.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAT2B1_HUMAN
AccessioniPrimary (citable) accession number: P20020
Secondary accession number(s): Q12992
, Q12993, Q13819, Q13820, Q13821, Q16504, Q93082
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 185 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.