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Protein

Isocitrate lyase

Gene

ICL1

Organism
Candida tropicalis (Yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle. Required for growth on ethanol or acetate, but dispensable when fermentable carbon sources are available. Acts also on 2-methylisocitrate.By similarity

Catalytic activityi

Isocitrate = succinate + glyoxylate.By similarity
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate.By similarity

Cofactori

Mg2+By similarity

Pathwayi: glyoxylate cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from isocitrate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Isocitrate lyase (ICL1)
  2. Malate synthase, glyoxysomal (PMS1)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi172MagnesiumBy similarity1
Active sitei210Proton acceptorBy similarity1
Binding sitei247SubstrateBy similarity1
Binding sitei464SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate lyase1 Publication (EC:4.1.3.1By similarity)
Short name:
ICLCurated
Short name:
IsocitraseCurated
Short name:
IsocitrataseCurated
Alternative name(s):
Methylisocitrate lyaseBy similarity (EC:4.1.3.30By similarity)
Short name:
MICACurated
Threo-D(S)-isocitrate glyoxylate-lyaseCurated
Gene namesi
Name:ICL11 Publication
OrganismiCandida tropicalis (Yeast)
Taxonomic identifieri5482 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000687861 – 550Isocitrate lyaseAdd BLAST550

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP20014.
SMRiP20014.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni101 – 103Substrate bindingBy similarity3
Regioni211 – 212Substrate bindingBy similarity2
Regioni430 – 434Substrate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi548 – 550Microbody targeting signalSequence analysis3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1260. Eukaryota.
COG2224. LUCA.

Family and domain databases

InterProiView protein in InterPro
IPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
PANTHERiPTHR21631. PTHR21631. 1 hit.
PfamiView protein in Pfam
PF00463. ICL. 1 hit.
PIRSFiPIRSF001362. Isocit_lyase. 1 hit.
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiView protein in PROSITE
PS00161. ISOCITRATE_LYASE. 1 hit.

Sequencei

Sequence statusi: Complete.

P20014-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYTKIDINQ EEADFQKEVA EIKKWWSEPR WRKTKRIYSA EDIAKKRGTL
60 70 80 90 100
KIAYPSSQQS DKLFKLLEKH DAEKSVSFTF GALDPIHVAQ MAKYLDSIYV
110 120 130 140 150
SGWQCSSTAS TSNEPSPDLA DYPMDTVPNK VEHLWFAQLF HDRKQREERL
160 170 180 190 200
NMTKEERANT PYIDFLRPII ADADTGHGGI TAIIKLTKLF IERGAAGIHI
210 220 230 240 250
EDQAPGTKKC GHMAGKVLVP VQEHINRLVA IRASADIFGS NLLAVARTDS
260 270 280 290 300
EAATLITSTI DHRDHYFIIG ATNPESGDLA ALMAEAEAKG IYGDELARIE
310 320 330 340 350
TEWTKKAGLK LFHEAVIDEI KAGNYSNKEA LIKKFTDKVN PLSHTSHKEA
360 370 380 390 400
KKLAKELTGK DIYFNWDVAR AREGYYRYQG GTQCAVMRGR AFAPYADLIW
410 420 430 440 450
MESALPDYNQ AKEFADGVKA AVPDQWLAYN LSPSFNWNKA MPADEQETYI
460 470 480 490 500
KRLGQLGYVW QFITLAGLHT TALAVDDFAN QYSQIGMRAY GQTVQQPEIE
510 520 530 540 550
KGVEVVKHQK WSGANYIDGL LRMVSGGVTS TAAMGAGVTE DQFKETKAKV
Length:550
Mass (Da):61,577
Last modified:February 1, 1991 - v1
Checksum:iBA86F18169B5AC0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00703 Genomic DNA. Translation: BAA00611.1.
PIRiJX0105. WZCKI.

Similar proteinsi

Entry informationi

Entry nameiACEA_CANTR
AccessioniPrimary (citable) accession number: P20014
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: August 30, 2017
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families