P20013 (LIGC_TRAVE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Ligninase C EC=1.11.1.14 Alternative name(s): Diarylpropane peroxidase Lignin peroxidase
Organism	Trametes versicolor (White-rot fungus) (Coriolus versicolor)
Taxonomic identifier	5325 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Aphyllophorales › Trametes

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Depolymerization of lignin. Catalyzes the C(alpha)-C(beta) cleavage of the propyl side chains of lignin.
Catalytic activity	(3,4-dimethoxyphenyl)methanol + H2O2 = 3,4-dimethoxybenzaldehyde + 2 H2O.
Cofactor	Binds 2 calcium ions per subunit By similarity.
Pathway	Secondary metabolite metabolism; lignin degradation.
Developmental stage	Ligninases are expressed during secondary metabolism, and are triggered by nutrient limitation.
Sequence similarities	Belongs to the peroxidase family. Ligninase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide Lignin degradation
Domain	Signal
Ligand	Calcium Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	diarylpropane peroxidase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Ref.2
	Chain	27 – 372	346	Ligninase C		PRO_0000023777
Sites
	Active site	74	1	Proton acceptor By similarity
	Metal binding	75	1	Calcium 1 By similarity
	Metal binding	93	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	95	1	Calcium 1 By similarity
	Metal binding	97	1	Calcium 1 By similarity
	Metal binding	205	1	Iron (heme axial ligand) By similarity
	Metal binding	206	1	Calcium 2 By similarity
	Metal binding	223	1	Calcium 2 By similarity
	Metal binding	225	1	Calcium 2 By similarity
	Metal binding	228	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	230	1	Calcium 2 By similarity
	Site	70	1	Transition state stabilizer By similarity
Amino acid modifications
	Glycosylation	129	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P20013 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: 9F7A06AABAA9B25A	FASTA	372	39,625
	10 20 30 40 50 60 MAFKSLLSFV SVIGALQGAN AALTRRVACP DGVNTATNAA CCQLFAVRED LQQNLFHGGL 70 80 90 100 110 120 CTAEAHESLR LTFHDAIAIS PALEAQGIFG GGGADGSIAI FPEIETNFHP NIGLDEIIEL 130 140 150 160 170 180 QKPFIARHNI SVADFIQFAG AIGASNCAGA PQLAAFVGRK DATQPAPDGL VPEPFHTPDQ 190 200 210 220 230 240 IFDRLADASQ GEFDPILTVW LLTAHTVAAA NDVDPTKSGL PFDSTPELWD TQFFLETQLR 250 260 270 280 290 300 GTSFPGSGGN QGEVESPLAG EMRLQSDHTI ARDSRTACEW QSFVDNQPKA QQMFQFVFHD 310 320 330 340 350 360 LSIFGQDINT LVDCTEVVPI PADPQGHTHF PAGLSNADIE QACAETPFPT FPTDPGPKTA 370 VAPVPKPPAA RK

References

[1]	"Characterization of a lignin peroxidase gene from the white-rot fungus Trametes versicolor." Joensson L.J., Nyman P.O. Biochimie 74:177-182(1992) [PubMed: 1581393] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PRL 572.
[2]	"Trametes versicolor ligninase: isozyme sequence homology and substrate specificity." Joensson L.J., Karlsson O., Lundquist K., Nyman P.O. FEBS Lett. 247:143-146(1989) [PubMed: 2707445] [Abstract] Cited for: PROTEIN SEQUENCE OF 27-38.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M64993 Genomic DNA. Translation: AAA34049.1.
PIR	S32581.
3D structure databases
ProteinModelPortal	P20013.
ModBase	Search...
Protein family/group databases
PeroxiBase	2420. TvLiP12_PRL572.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR001621. Ligninase. IPR024589. Ligninase_C. IPR019794. Peroxidases_AS. [Graphical view]
Pfam	PF11895. DUF3415. 1 hit. PF00141. peroxidase. 1 hit. [Graphical view]
PRINTS	PR00462. LIGNINASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIGC_TRAVE

Accession

Primary (citable) accession number: P20013

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	June 1, 1994
Last modified:	December 14, 2011
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program