P20010 (LIG_PHLRA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Ligninase-3 EC=1.11.1.14 Alternative name(s): Diarylpropane peroxidase Lignin peroxidase Ligninase III
Organism	Phlebia radiata (White-rot fungus)
Taxonomic identifier	5308 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Corticiales › Corticiaceae › Phlebia

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Depolymerization of lignin. Catalyzes the C(alpha)-C(beta) cleavage of the propyl side chains of lignin.
Catalytic activity	(3,4-dimethoxyphenyl)methanol + H2O2 = 3,4-dimethoxybenzaldehyde + 2 H2O.
Cofactor	Binds 2 calcium ions per subunit By similarity.
Pathway	Secondary metabolite metabolism; lignin degradation.
Sequence similarities	Belongs to the peroxidase family. Ligninase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide Lignin degradation
Domain	Signal
Ligand	Calcium Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	diarylpropane peroxidase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 361	341	Ligninase-3		PRO_0000023776
Sites
	Active site	70	1	Proton acceptor By similarity
	Metal binding	71	1	Calcium 1 By similarity
	Metal binding	89	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	91	1	Calcium 1 By similarity
	Metal binding	93	1	Calcium 1 By similarity
	Metal binding	199	1	Iron (heme axial ligand) By similarity
	Metal binding	200	1	Calcium 2 By similarity
	Metal binding	217	1	Calcium 2 By similarity
	Metal binding	219	1	Calcium 2 By similarity
	Metal binding	222	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	224	1	Calcium 2 By similarity
	Site	66	1	Transition state stabilizer By similarity
Amino acid modifications
	Glycosylation	268	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P20010 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: A353806643242974	FASTA	361	38,439
	10 20 30 40 50 60 MAFKQLLSAV TLALAASAAS VTRRATCPDG TQLMNAECCA LLAVRDDLQN NMFNNECGDE 70 80 90 100 110 120 AHEALRLTFH DAIAISPAME ATGQFGGGGA DGSIMIFSDI ETKFHPNIGL DEVVESFRPF 130 140 150 160 170 180 QQRSGMGVAD FIQFSGAVGT SNCPGAPTLN AFIGRKDATQ AAPDGLVPEP FHDVNTILAR 190 200 210 220 230 240 FNDAGDFDEL ETVWFLIAHS VAAQNDIDPA VSHAPFDSTP SVMDGQFFIE TQLRGVEFIG 250 260 270 280 290 300 SGGIEGVAES PVKGEFRLMS DQQIARDNRT ACEWQSFGTD QAKLQNRFQF IFEAMGQLGT 310 320 330 340 350 360 DPTTLIDCSD VLPVPPPLST VPHFPAGITI NDVEPACAET PFPTLPTDPG PATAVAAVPR D

References

[1]	"A lignin peroxidase-encoding cDNA from the white-rot fungus Phlebia radiata: characterization and expression in Trichoderma reesei." Saloheimo M., Barajas V., Niku-Paavola M.L., Knowles J.K.C. Gene 85:343-351(1989) [PubMed: 2628172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 64658 / 79.
+	Additional computationally mapped references.

Cross-references

Sequence databases
PIR	JQ0374.
3D structure databases
ProteinModelPortal	P20010.
SMR	P20010. Positions 21-359.
ModBase	Search...
Protein family/group databases
PeroxiBase	2297. PrLiP03.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR001621. Ligninase. IPR024589. Ligninase_C. IPR019794. Peroxidases_AS. [Graphical view]
Pfam	PF11895. DUF3415. 1 hit. PF00141. peroxidase. 1 hit. [Graphical view]
PRINTS	PR00462. LIGNINASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIG_PHLRA

Accession

Primary (citable) accession number: P20010

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	October 19, 2011
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program