Skip Header

 
Contribute Send feedback
Read comments (1) or add your own

Reviewed, UniProtKB/Swiss-Prot P20010 (LIG_PHLRA)

Last modified May 26, 2009. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Ligninase-3
    EC=1.11.1.14
Alternative name(s):
    Ligninase III
    Diarylpropane peroxidase
    Lignin peroxidase
OrganismPhlebia radiata (White-rot fungus)
Taxonomic identifier5308 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaHomobasidiomycetesCorticialesCorticiaceaePhlebia

Protein attributes

Sequence length361 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Depolymerization of lignin. Catalyzes the C(alpha)-C(beta) cleavage of the propyl side chains of lignin.

Catalytic activity

1,2-bis(3,4-dimethoxyphenyl)propane-1,3-diol + H2O2 = 3,4-dimethoxybenzaldehyde + 1-(3,4-dimethoxyphenyl)ethane-1,2-diol + H2O.

Cofactor

Binds 2 calcium ions per subunit By similarity.

Pathway

Secondary metabolite metabolism; lignin degradation.

Sequence similarities

Belongs to the peroxidase family. Ligninase subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 361341Ligninase-3
PRO_0000023776

Sites

Active site701Proton acceptor By similarity
Metal binding711Calcium 1 By similarity
Metal binding891Calcium 1; via carbonyl oxygen By similarity
Metal binding911Calcium 1 By similarity
Metal binding931Calcium 1 By similarity
Metal binding1991Iron (heme axial ligand) By similarity
Metal binding2001Calcium 2 By similarity
Metal binding2171Calcium 2 By similarity
Metal binding2191Calcium 2 By similarity
Metal binding2221Calcium 2; via carbonyl oxygen By similarity
Metal binding2241Calcium 2 By similarity
Site661Transition state stabilizer By similarity

Amino acid modifications

Glycosylation2681N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P20010-1 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: A353806643242974

FASTA36138,439
        10         20         30         40         50         60 
MAFKQLLSAV TLALAASAAS VTRRATCPDG TQLMNAECCA LLAVRDDLQN NMFNNECGDE 

        70         80         90        100        110        120 
AHEALRLTFH DAIAISPAME ATGQFGGGGA DGSIMIFSDI ETKFHPNIGL DEVVESFRPF 

       130        140        150        160        170        180 
QQRSGMGVAD FIQFSGAVGT SNCPGAPTLN AFIGRKDATQ AAPDGLVPEP FHDVNTILAR 

       190        200        210        220        230        240 
FNDAGDFDEL ETVWFLIAHS VAAQNDIDPA VSHAPFDSTP SVMDGQFFIE TQLRGVEFIG 

       250        260        270        280        290        300 
SGGIEGVAES PVKGEFRLMS DQQIARDNRT ACEWQSFGTD QAKLQNRFQF IFEAMGQLGT 

       310        320        330        340        350        360 
DPTTLIDCSD VLPVPPPLST VPHFPAGITI NDVEPACAET PFPTLPTDPG PATAVAAVPR 


D 

« Hide

References

[1]"A lignin peroxidase-encoding cDNA from the white-rot fungus Phlebia radiata: characterization and expression in Trichoderma reesei."
Saloheimo M., Barajas V., Niku-Paavola M.L., Knowles J.K.C.
Gene 85:343-351(1989) [PubMed: 2628172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: ATCC 64658 / 79.

Cross-references

Sequence databases

PIRJQ0374.

3D structure databases

HSSPHSSP built from PDB template 1B85 based on UniProtKB P06181.
ModBaseSearch...

Protein family/group databases

PeroxiBase2297. PrLiP03.

Enzyme and pathway databases

BRENDA1.11.1.14. 258600.

Family and domain databases

InterProIPR002016. Haem_peroxidase_pln/fun/bac.
IPR001621. Ligninase.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSPR00462. LIGNINASE.
PR00458. PEROXIDASE.
PROSITEPS00435. PEROXIDASE_1. False negative.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLIG_PHLRA
AccessionPrimary (citable) accession number: P20010
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: May 26, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents