Ligninase-3 precursor - Phlebia radiata (White-rot fungus)

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Reviewed, UniProtKB/Swiss-Prot P20010 (LIG_PHLRA)

Last modified May 26, 2009. Version 63. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names	Recommended name: Ligninase-3 EC=1.11.1.14 Alternative name(s): Ligninase III Diarylpropane peroxidase Lignin peroxidase
Organism	Phlebia radiata (White-rot fungus)
Taxonomic identifier	5308 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Corticiales › Corticiaceae › Phlebia

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Depolymerization of lignin. Catalyzes the C(alpha)-C(beta) cleavage of the propyl side chains of lignin.
Catalytic activity	1,2-bis(3,4-dimethoxyphenyl)propane-1,3-diol + H₂O₂ = 3,4-dimethoxybenzaldehyde + 1-(3,4-dimethoxyphenyl)ethane-1,2-diol + H₂O.
Cofactor	Binds 2 calcium ions per subunit By similarity.
Pathway	Secondary metabolite metabolism; lignin degradation.
Sequence similarities	Belongs to the peroxidase family. Ligninase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide Lignin degradation
Domain	Signal
Ligand	Calcium Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW diarylpropane peroxidase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

20

Potential

Chain

341

Ligninase-3

PRO_0000023776

Sites

Active site

1

Proton acceptor By similarity

Metal binding

1

Calcium 1 By similarity

Metal binding

1

Calcium 1; via carbonyl oxygen By similarity

Metal binding

1

Calcium 1 By similarity

Metal binding

1

Calcium 1 By similarity

Metal binding

1

Iron (heme axial ligand) By similarity

Metal binding

1

Calcium 2 By similarity

Metal binding

1

Calcium 2 By similarity

Metal binding

1

Calcium 2 By similarity

Metal binding

1

Calcium 2; via carbonyl oxygen By similarity

Metal binding

1

Calcium 2 By similarity

Site

1

Transition state stabilizer By similarity

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P20010-1 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: A353806643242974
Show »

361

38,439

        10         20         30         40         50         60 
MAFKQLLSAV TLALAASAAS VTRRATCPDG TQLMNAECCA LLAVRDDLQN NMFNNECGDE 

        70         80         90        100        110        120 
AHEALRLTFH DAIAISPAME ATGQFGGGGA DGSIMIFSDI ETKFHPNIGL DEVVESFRPF 

       130        140        150        160        170        180 
QQRSGMGVAD FIQFSGAVGT SNCPGAPTLN AFIGRKDATQ AAPDGLVPEP FHDVNTILAR 

       190        200        210        220        230        240 
FNDAGDFDEL ETVWFLIAHS VAAQNDIDPA VSHAPFDSTP SVMDGQFFIE TQLRGVEFIG 

       250        260        270        280        290        300 
SGGIEGVAES PVKGEFRLMS DQQIARDNRT ACEWQSFGTD QAKLQNRFQF IFEAMGQLGT 

       310        320        330        340        350        360 
DPTTLIDCSD VLPVPPPLST VPHFPAGITI NDVEPACAET PFPTLPTDPG PATAVAAVPR 


D

References

[1]

"A lignin peroxidase-encoding cDNA from the white-rot fungus Phlebia radiata: characterization and expression in Trichoderma reesei."
Saloheimo M., Barajas V., Niku-Paavola M.L., Knowles J.K.C.
Gene 85:343-351(1989) [PubMed: 2628172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: ATCC 64658 / 79.

Cross-references

Sequence databases
PIR	JQ0374.
3D structure databases
HSSP	HSSP built from PDB template 1B85 based on UniProtKB P06181.
ModBase	Search...
Protein family/group databases
PeroxiBase	2297. PrLiP03.
Enzyme and pathway databases
BRENDA	1.11.1.14. 258600.
Family and domain databases
InterPro	IPR002016. Haem_peroxidase_pln/fun/bac. IPR001621. Ligninase. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 1 hit. [Graphical view]
PRINTS	PR00462. LIGNINASE. PR00458. PEROXIDASE.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIG_PHLRA

Accession

Primary (citable) accession number: P20010

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	May 26, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents