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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

Pepck

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.By similarity

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

Cofactori

Mn2+By similarityNote: Binds 1 Mn2+ ion per subunit.By similarity

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei112SubstrateBy similarity1
Metal bindingi270ManganeseBy similarity1
Metal bindingi290Manganese; via tele nitrogenBy similarity1
Binding sitei312SubstrateBy similarity1
Active sitei314By similarity1
Metal bindingi337ManganeseBy similarity1
Binding sitei431GTPBy similarity1
Binding sitei462GTPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi313 – 318GTPBy similarity6
Nucleotide bindingi554 – 557GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processGluconeogenesis
LigandGTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-70263 Gluconeogenesis
UniPathwayiUPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
Short name:
PEPCK
Gene namesi
Name:Pepck
Synonyms:ZDF4
ORF Names:CG17725
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0003067 Pepck

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001036351 – 647Phosphoenolpyruvate carboxykinase [GTP]Add BLAST647

Proteomic databases

PaxDbiP20007
PRIDEiP20007

Expressioni

Gene expression databases

BgeeiFBgn0003067
GenevisibleiP20007 DM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi628065 interactors.
DIPiDIP-22737N
IntActiP20007 3 interactors.
STRINGi7227.FBpp0085880

Structurei

3D structure databases

ProteinModelPortaliP20007
SMRiP20007
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni261 – 263Substrate bindingBy similarity3
Regioni429 – 431Substrate bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3749 Eukaryota
COG1274 LUCA
GeneTreeiENSGT00390000001912
InParanoidiP20007
KOiK01596
OMAiCWLALTD
OrthoDBiEOG091G02YK
PhylomeDBiP20007

Family and domain databases

CDDicd00819 PEPCK_GTP, 1 hit
Gene3Di3.40.449.102 hits
3.90.228.202 hits
HAMAPiMF_00452 PEPCK_GTP, 1 hit
InterProiView protein in InterPro
IPR018091 PEP_carboxykin_GTP_CS
IPR013035 PEP_carboxykinase_C
IPR008209 PEP_carboxykinase_GTP
IPR035077 PEP_carboxykinase_GTP_C
IPR035078 PEP_carboxykinase_GTP_N
IPR008210 PEP_carboxykinase_N
PANTHERiPTHR11561 PTHR11561, 1 hit
PfamiView protein in Pfam
PF00821 PEPCK_C, 1 hit
PF17297 PEPCK_N, 1 hit
PIRSFiPIRSF001348 PEP_carboxykinase_GTP, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit
PROSITEiView protein in PROSITE
PS00505 PEPCK_GTP, 1 hit

Sequencei

Sequence statusi: Complete.

P20007-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPELIEQSKI ISGNVCGLPQ LHKLRQDNCG LYSHIRGIPI SYGNVDLLTT
60 70 80 90 100
GVRAFVEEGI ALCQPDQVHI CDGSEQENKV LIKSLLEAGT IVPLPKYDNC
110 120 130 140 150
WLARTNPADV ARVESRTFIC TERREETIPT PVEGVKGTLG NWISPSDMDA
160 170 180 190 200
AVQQRFPGCM KGRTMYVVPF SMGPVGSPLS KIGIELTDSA YVVASMRIMT
210 220 230 240 250
RMGAAVLRQL AKKEEFVRAL HSVGAPANGQ VEQPSWPCDP ERTIILHKPA
260 270 280 290 300
ENLIVSYGSG YGGNSLLGKK CFALRIGSTI AKQEGWLAEH MLILGITDPK
310 320 330 340 350
GEKKYITAAF PSACGKTNLA MLNPSLANYK VECVGDDIAW MKFDSQGVLR
360 370 380 390 400
AINPENGFFG VAPGTSMETN PIAMNTVFKN TIFTNVASTS DGGVFWEGME
410 420 430 440 450
SSLAPNVQIT DWLGKPWTKD SGKPAAHPNS RFCTPAAQCP IIDEAWEDPA
460 470 480 490 500
GVPISAMLFG GRRPAGVPLI YEARDWTHGV FIGAAMRSEA TAAAEHKGKV
510 520 530 540 550
IMHDPFAMRP FFGYNFGDYV AHWLSMEKRG QVPKIFHVNW FRKSAEGKFM
560 570 580 590 600
WPGYGENSRV LEWILRRVNG ESCYVDSAIG HIPAEGALNL DGMKDKVDVK
610 620 630 640
EIFSLPKEFW SQEVKDIRTY FESQVGADLP ASIYQQLDEL SSRVDNL
Length:647
Mass (Da):71,129
Last modified:December 1, 2000 - v2
Checksum:i0DB81B3D9E1B1FBB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti302E → V in CAA68463 (PubMed:3114718).Curated1
Sequence conflicti408Q → R in CAA68463 (PubMed:3114718).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00402 mRNA Translation: CAA68463.1
AE013599 Genomic DNA Translation: AAF57676.1
BT003447 mRNA Translation: AAO39450.1
PIRiA26809 QYFFGM
RefSeqiNP_523784.2, NM_079060.3
UniGeneiDm.6919

Genome annotation databases

EnsemblMetazoaiFBtr0086701; FBpp0085880; FBgn0003067
GeneIDi37131
KEGGidme:Dmel_CG17725

Similar proteinsi

Entry informationi

Entry nameiPCKG_DROME
AccessioniPrimary (citable) accession number: P20007
Secondary accession number(s): A1ZB97, Q53YF8, Q9V8J0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: December 1, 2000
Last modified: March 28, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome