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P20007

- PCKG_DROME

UniProt

P20007 - PCKG_DROME

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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

Pepck

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.By similarity

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

Cofactori

Binds 1 manganese ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei112 – 1121SubstrateBy similarity
Binding sitei263 – 2631Substrate; via amide nitrogenBy similarity
Metal bindingi270 – 2701ManganeseBy similarity
Binding sitei270 – 2701SubstrateBy similarity
Metal bindingi290 – 2901Manganese; via tele nitrogenBy similarity
Binding sitei312 – 3121SubstrateBy similarity
Active sitei314 – 3141By similarity
Metal bindingi337 – 3371ManganeseBy similarity
Binding sitei431 – 4311GTPBy similarity
Binding sitei462 – 4621GTPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi313 – 3186GTPBy similarity
Nucleotide bindingi554 – 5574GTPBy similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-EC

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_184366. Abacavir metabolism.
REACT_220315. Gluconeogenesis.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
Short name:
PEPCK
Gene namesi
Name:Pepck
Synonyms:ZDF4
ORF Names:CG17725
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0003067. Pepck.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 647647Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103635Add
BLAST

Proteomic databases

PaxDbiP20007.
PRIDEiP20007.

Expressioni

Gene expression databases

BgeeiP20007.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi62806. 3 interactions.
DIPiDIP-22737N.
MINTiMINT-762297.
STRINGi7227.FBpp0085880.

Structurei

3D structure databases

ProteinModelPortaliP20007.
SMRiP20007. Positions 43-647.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni429 – 4313Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1274.
GeneTreeiENSGT00390000001912.
InParanoidiP20007.
KOiK01596.
OMAiPDHIHIC.
OrthoDBiEOG7KSX81.
PhylomeDBiP20007.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform A (identifier: P20007-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPELIEQSKI ISGNVCGLPQ LHKLRQDNCG LYSHIRGIPI SYGNVDLLTT
60 70 80 90 100
GVRAFVEEGI ALCQPDQVHI CDGSEQENKV LIKSLLEAGT IVPLPKYDNC
110 120 130 140 150
WLARTNPADV ARVESRTFIC TERREETIPT PVEGVKGTLG NWISPSDMDA
160 170 180 190 200
AVQQRFPGCM KGRTMYVVPF SMGPVGSPLS KIGIELTDSA YVVASMRIMT
210 220 230 240 250
RMGAAVLRQL AKKEEFVRAL HSVGAPANGQ VEQPSWPCDP ERTIILHKPA
260 270 280 290 300
ENLIVSYGSG YGGNSLLGKK CFALRIGSTI AKQEGWLAEH MLILGITDPK
310 320 330 340 350
GEKKYITAAF PSACGKTNLA MLNPSLANYK VECVGDDIAW MKFDSQGVLR
360 370 380 390 400
AINPENGFFG VAPGTSMETN PIAMNTVFKN TIFTNVASTS DGGVFWEGME
410 420 430 440 450
SSLAPNVQIT DWLGKPWTKD SGKPAAHPNS RFCTPAAQCP IIDEAWEDPA
460 470 480 490 500
GVPISAMLFG GRRPAGVPLI YEARDWTHGV FIGAAMRSEA TAAAEHKGKV
510 520 530 540 550
IMHDPFAMRP FFGYNFGDYV AHWLSMEKRG QVPKIFHVNW FRKSAEGKFM
560 570 580 590 600
WPGYGENSRV LEWILRRVNG ESCYVDSAIG HIPAEGALNL DGMKDKVDVK
610 620 630 640
EIFSLPKEFW SQEVKDIRTY FESQVGADLP ASIYQQLDEL SSRVDNL
Length:647
Mass (Da):71,129
Last modified:December 1, 2000 - v2
Checksum:i0DB81B3D9E1B1FBB
GO
Isoform B (identifier: P20007-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-147: Missing.

Note: No experimental confirmation available.

Show »
Length:500
Mass (Da):54,949
Checksum:iF49B9BEAD8D0E15E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti302 – 3021E → V in CAA68463. (PubMed:3114718)Curated
Sequence conflicti408 – 4081Q → R in CAA68463. (PubMed:3114718)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 147147Missing in isoform B. CuratedVSP_026173Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y00402 mRNA. Translation: CAA68463.1.
AE013599 Genomic DNA. Translation: AAF57676.1.
AE013599 Genomic DNA. Translation: AAM68462.1.
BT003447 mRNA. Translation: AAO39450.1.
PIRiA26809. QYFFGM.
RefSeqiNP_523784.2. NM_079060.3. [P20007-1]
UniGeneiDm.6919.

Genome annotation databases

EnsemblMetazoaiFBtr0086701; FBpp0085880; FBgn0003067. [P20007-1]
GeneIDi37131.
KEGGidme:Dmel_CG17725.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y00402 mRNA. Translation: CAA68463.1 .
AE013599 Genomic DNA. Translation: AAF57676.1 .
AE013599 Genomic DNA. Translation: AAM68462.1 .
BT003447 mRNA. Translation: AAO39450.1 .
PIRi A26809. QYFFGM.
RefSeqi NP_523784.2. NM_079060.3. [P20007-1 ]
UniGenei Dm.6919.

3D structure databases

ProteinModelPortali P20007.
SMRi P20007. Positions 43-647.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 62806. 3 interactions.
DIPi DIP-22737N.
MINTi MINT-762297.
STRINGi 7227.FBpp0085880.

Proteomic databases

PaxDbi P20007.
PRIDEi P20007.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0086701 ; FBpp0085880 ; FBgn0003067 . [P20007-1 ]
GeneIDi 37131.
KEGGi dme:Dmel_CG17725.

Organism-specific databases

CTDi 37131.
FlyBasei FBgn0003067. Pepck.

Phylogenomic databases

eggNOGi COG1274.
GeneTreei ENSGT00390000001912.
InParanoidi P20007.
KOi K01596.
OMAi PDHIHIC.
OrthoDBi EOG7KSX81.
PhylomeDBi P20007.

Enzyme and pathway databases

UniPathwayi UPA00138 .
Reactomei REACT_184366. Abacavir metabolism.
REACT_220315. Gluconeogenesis.

Miscellaneous databases

ChiTaRSi PCK2. drosophila.
GenomeRNAii 37131.
NextBioi 802101.

Gene expression databases

Bgeei P20007.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide and deduced amino acid sequence of the phosphoenolpyruvate carboxykinase (GTP) from Drosophila melanogaster."
    Gundelfinger E.D., Hermans-Borgmeyer I., Grenningloh G., Zopf D.
    Nucleic Acids Res. 15:6745-6745(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
    Strain: Canton-S.
    Tissue: Head.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
    Strain: Berkeley.
    Tissue: Head.

Entry informationi

Entry nameiPCKG_DROME
AccessioniPrimary (citable) accession number: P20007
Secondary accession number(s): A1ZB97, Q53YF8, Q9V8J0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: December 1, 2000
Last modified: October 29, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3