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Protein

Aconitate hydratase, mitochondrial

Gene

ACO2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.

Catalytic activityi

Citrate = isocitrate.

Cofactori

[4Fe-4S] cluster1 PublicationNote: Binds 1 [4Fe-4S] cluster per subunit. Binding of a [3Fe-4S] cluster leads to an inactive enzyme.1 Publication

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 2 of the subpathway that synthesizes isocitrate from oxaloacetate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase, mitochondrial (CS)
  2. Aconitate hydratase, mitochondrial (ACO2)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99Substrate1
Metal bindingi385Iron-sulfur (4Fe-4S)2 Publications1
Metal bindingi448Iron-sulfur (4Fe-4S)2 Publications1
Metal bindingi451Iron-sulfur (4Fe-4S)2 Publications1
Binding sitei474Substrate1
Binding sitei479Substrate1
Binding sitei607Substrate1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-71403. Citric acid cycle (TCA cycle).
UniPathwayiUPA00223; UER00718.

Names & Taxonomyi

Protein namesi
Recommended name:
Aconitate hydratase, mitochondrial (EC:4.2.1.3)
Short name:
Aconitase
Alternative name(s):
Citrate hydro-lyase
Gene namesi
Name:ACO2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 5

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 27MitochondrionAdd BLAST27
ChainiPRO_000000054028 – 780Aconitate hydratase, mitochondrialAdd BLAST753

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei28Pyrrolidone carboxylic acidBy similarity1
Modified residuei31N6-succinyllysineBy similarity1
Modified residuei50N6-acetyllysine; alternateBy similarity1
Modified residuei50N6-succinyllysine; alternateBy similarity1
Modified residuei138N6-acetyllysine; alternateBy similarity1
Modified residuei138N6-succinyllysine; alternateBy similarity1
Modified residuei144N6-acetyllysine; alternateBy similarity1
Modified residuei144N6-succinyllysine; alternateBy similarity1
Modified residuei233N6-acetyllysine; alternateBy similarity1
Modified residuei233N6-succinyllysine; alternateBy similarity1
Modified residuei411N6-succinyllysineBy similarity1
Modified residuei517N6-acetyllysine; alternateBy similarity1
Modified residuei517N6-succinyllysine; alternateBy similarity1
Modified residuei523N6-acetyllysine; alternateBy similarity1
Modified residuei523N6-succinyllysine; alternateBy similarity1
Modified residuei549N6-succinyllysineBy similarity1
Modified residuei559PhosphoserineBy similarity1
Modified residuei573N6-acetyllysine; alternateBy similarity1
Modified residuei573N6-succinyllysine; alternateBy similarity1
Modified residuei591N6-succinyllysineBy similarity1
Modified residuei605N6-acetyllysine; alternateBy similarity1
Modified residuei605N6-succinyllysine; alternateBy similarity1
Modified residuei628N6-succinyllysineBy similarity1
Modified residuei670PhosphoserineBy similarity1
Modified residuei689N6-succinyllysineBy similarity1
Modified residuei723N6-acetyllysine; alternateBy similarity1
Modified residuei723N6-succinyllysine; alternateBy similarity1
Modified residuei730N6-acetyllysine; alternateBy similarity1
Modified residuei730N6-succinyllysine; alternateBy similarity1
Modified residuei736N6-acetyllysineBy similarity1
Modified residuei743N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP20004.
PeptideAtlasiP20004.
PRIDEiP20004.

Expressioni

Gene expression databases

BgeeiENSBTAG00000006429.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

IntActiP20004. 1 interactor.
STRINGi9913.ENSBTAP00000008431.

Structurei

Secondary structure

1780
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi33 – 35Combined sources3
Beta strandi38 – 42Combined sources5
Helixi45 – 59Combined sources15
Helixi65 – 71Combined sources7
Turni77 – 79Combined sources3
Turni84 – 86Combined sources3
Beta strandi88 – 91Combined sources4
Beta strandi94 – 99Combined sources6
Turni100 – 102Combined sources3
Helixi103 – 113Combined sources11
Beta strandi122 – 125Combined sources4
Beta strandi132 – 134Combined sources3
Helixi136 – 146Combined sources11
Helixi148 – 161Combined sources14
Beta strandi164 – 166Combined sources3
Helixi173 – 180Combined sources8
Beta strandi187 – 192Combined sources6
Helixi195 – 201Combined sources7
Beta strandi204 – 207Combined sources4
Helixi210 – 218Combined sources9
Beta strandi222 – 225Combined sources4
Beta strandi228 – 236Combined sources9
Helixi244 – 255Combined sources12
Turni256 – 262Combined sources7
Beta strandi263 – 269Combined sources7
Helixi270 – 274Combined sources5
Helixi277 – 286Combined sources10
Helixi287 – 290Combined sources4
Beta strandi293 – 296Combined sources4
Helixi301 – 309Combined sources9
Helixi313 – 321Combined sources9
Helixi323 – 325Combined sources3
Beta strandi335 – 341Combined sources7
Helixi342 – 344Combined sources3
Beta strandi348 – 350Combined sources3
Beta strandi352 – 354Combined sources3
Beta strandi358 – 360Combined sources3
Helixi361 – 371Combined sources11
Beta strandi376 – 383Combined sources8
Turni384 – 386Combined sources3
Helixi390 – 404Combined sources15
Turni405 – 407Combined sources3
Beta strandi411 – 416Combined sources6
Helixi421 – 429Combined sources9
Helixi432 – 438Combined sources7
Beta strandi441 – 443Combined sources3
Helixi449 – 452Combined sources4
Beta strandi466 – 473Combined sources8
Turni477 – 481Combined sources5
Beta strandi486 – 490Combined sources5
Helixi493 – 502Combined sources10
Beta strandi504 – 506Combined sources3
Turni509 – 511Combined sources3
Beta strandi513 – 515Combined sources3
Beta strandi521 – 523Combined sources3
Turni549 – 551Combined sources3
Beta strandi563 – 565Combined sources3
Beta strandi579 – 588Combined sources10
Helixi594 – 597Combined sources4
Helixi601 – 606Combined sources6
Helixi610 – 613Combined sources4
Helixi614 – 616Combined sources3
Turni618 – 620Combined sources3
Beta strandi621 – 623Combined sources3
Turni624 – 626Combined sources3
Turni635 – 637Combined sources3
Helixi643 – 652Combined sources10
Beta strandi657 – 660Combined sources4
Beta strandi663 – 665Combined sources3
Helixi673 – 680Combined sources8
Beta strandi683 – 689Combined sources7
Helixi693 – 701Combined sources9
Beta strandi705 – 711Combined sources7
Helixi712 – 717Combined sources6
Beta strandi723 – 727Combined sources5
Helixi729 – 731Combined sources3
Beta strandi738 – 743Combined sources6
Beta strandi749 – 755Combined sources7
Helixi760 – 768Combined sources9
Helixi771 – 778Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ACOX-ray2.05A29-779[»]
1AMIX-ray2.00A28-780[»]
1AMJX-ray2.00A28-780[»]
1C96X-ray1.81A29-779[»]
1C97X-ray1.98A29-779[»]
1FGHX-ray2.05A29-779[»]
1NISX-ray2.05A29-779[»]
1NITX-ray2.05A29-779[»]
8ACNX-ray2.00A29-780[»]
ProteinModelPortaliP20004.
SMRiP20004.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20004.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni192 – 194Substrate binding3
Regioni670 – 671Substrate binding2

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0453. Eukaryota.
COG1048. LUCA.
GeneTreeiENSGT00860000133751.
HOGENOMiHOG000224293.
HOVERGENiHBG000248.
InParanoidiP20004.
KOiK01681.
OMAiPDLGHPI.
OrthoDBiEOG091G024R.
TreeFamiTF300627.

Family and domain databases

Gene3Di3.20.19.10. 1 hit.
3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR006248. Aconitase_mito-like.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
PF00694. Aconitase_C. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF52016. SSF52016. 1 hit.
SSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR01340. aconitase_mito. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20004-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPYSLLVSR LQKALGARQY HVASVLCQRA KVAMSHFEPN EYIRYDLLEK
60 70 80 90 100
NINIVRKRLN RPLTLSEKIV YGHLDDPANQ EIERGKTYLR LRPDRVAMQD
110 120 130 140 150
ATAQMAMLQF ISSGLPKVAV PSTIHCDHLI EAQLGGEKDL RRAKDINQEV
160 170 180 190 200
YNFLATAGAK YGVGFWRPGS GIIHQIILEN YAYPGVLLIG TDSHTPNGGG
210 220 230 240 250
LGGICIGVGG ADAVDVMAGI PWELKCPKVI GVKLTGSLSG WTSPKDVILK
260 270 280 290 300
VAGILTVKGG TGAIVEYHGP GVDSISCTGM ATICNMGAEI GATTSVFPYN
310 320 330 340 350
HRMKKYLSKT GRADIANLAD EFKDHLVPDS GCHYDQLIEI NLSELKPHIN
360 370 380 390 400
GPFTPDLAHP VAEVGSVAEK EGWPLDIRVG LIGSCTNSSY EDMGRSAAVA
410 420 430 440 450
KQALAHGLQC KSQFTITPGS EQIRATIERD GYAQILRDVG GIVLANACGP
460 470 480 490 500
CIGQWDRKDI KKGEKNTIVT SYNRNFTGRN DANPETHAFV TSPEIVTALA
510 520 530 540 550
IAGTLKFNPE TDFLTGKDGK KFKLEAPDAD ELPRAEFDPG QDTYQHPPKD
560 570 580 590 600
SSGQQVDVSP TSQRLQLLEP FDKWDGRDLE DLQILIKVKG KCTTDHISAA
610 620 630 640 650
GPWLKFRGHL DNISNNLLIG AINVENGKAN SVRNAVTQEF GPVPDTARYY
660 670 680 690 700
KKHGIRWVVI GDENYGEGSS REHAALEPRH LGGRAIITKS FARIHETNLK
710 720 730 740 750
KQGLLPLTFA DPADYNKIHP VDKLTIKGLK DFAPGKPLTC IIKHPNGTQE
760 770 780
TILLNHTFNE TQIEWFRAGS ALNRMKELQK
Length:780
Mass (Da):85,359
Last modified:July 11, 2006 - v4
Checksum:i91EB21B3923F16C5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40N → H AA sequence (PubMed:3372519).Curated1
Sequence conflicti53N → D AA sequence (PubMed:3372519).Curated1
Sequence conflicti99Q → R in CAA90177 (Ref. 1) Curated1
Sequence conflicti179E → G in AAI02643 (Ref. 2) Curated1
Sequence conflicti217M → T in CAA90177 (Ref. 1) Curated1
Sequence conflicti409Q → K AA sequence (PubMed:3372519).Curated1
Sequence conflicti435I → V AA sequence (PubMed:3372519).Curated1
Sequence conflicti555Q → R AA sequence (PubMed:3372519).Curated1
Sequence conflicti577R → K AA sequence (PubMed:3372519).Curated1
Sequence conflicti624V → S AA sequence (PubMed:3372519).Curated1
Sequence conflicti627G → R AA sequence (PubMed:3372519).Curated1
Sequence conflicti652K → Q AA sequence (PubMed:3372519).Curated1
Sequence conflicti674A → S AA sequence (PubMed:3372519).Curated1
Sequence conflicti680H → F AA sequence (PubMed:3372519).Curated1
Sequence conflicti727K → Q AA sequence (PubMed:3372519).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49931 mRNA. Translation: CAA90177.1.
BC102642 mRNA. Translation: AAI02643.1.
PIRiS57528.
RefSeqiNP_776402.1. NM_173977.3.
UniGeneiBt.5210.

Genome annotation databases

EnsembliENSBTAT00000008431; ENSBTAP00000008431; ENSBTAG00000006429.
GeneIDi280976.
KEGGibta:280976.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49931 mRNA. Translation: CAA90177.1.
BC102642 mRNA. Translation: AAI02643.1.
PIRiS57528.
RefSeqiNP_776402.1. NM_173977.3.
UniGeneiBt.5210.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ACOX-ray2.05A29-779[»]
1AMIX-ray2.00A28-780[»]
1AMJX-ray2.00A28-780[»]
1C96X-ray1.81A29-779[»]
1C97X-ray1.98A29-779[»]
1FGHX-ray2.05A29-779[»]
1NISX-ray2.05A29-779[»]
1NITX-ray2.05A29-779[»]
8ACNX-ray2.00A29-780[»]
ProteinModelPortaliP20004.
SMRiP20004.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP20004. 1 interactor.
STRINGi9913.ENSBTAP00000008431.

Proteomic databases

PaxDbiP20004.
PeptideAtlasiP20004.
PRIDEiP20004.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000008431; ENSBTAP00000008431; ENSBTAG00000006429.
GeneIDi280976.
KEGGibta:280976.

Organism-specific databases

CTDi50.

Phylogenomic databases

eggNOGiKOG0453. Eukaryota.
COG1048. LUCA.
GeneTreeiENSGT00860000133751.
HOGENOMiHOG000224293.
HOVERGENiHBG000248.
InParanoidiP20004.
KOiK01681.
OMAiPDLGHPI.
OrthoDBiEOG091G024R.
TreeFamiTF300627.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00718.
ReactomeiR-BTA-71403. Citric acid cycle (TCA cycle).

Miscellaneous databases

EvolutionaryTraceiP20004.

Gene expression databases

BgeeiENSBTAG00000006429.

Family and domain databases

Gene3Di3.20.19.10. 1 hit.
3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR006248. Aconitase_mito-like.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
PF00694. Aconitase_C. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF52016. SSF52016. 1 hit.
SSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR01340. aconitase_mito. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACON_BOVIN
AccessioniPrimary (citable) accession number: P20004
Secondary accession number(s): Q3SZZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: July 11, 2006
Last modified: November 30, 2016
This is version 150 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.