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P20004

- ACON_BOVIN

UniProt

P20004 - ACON_BOVIN

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Protein
Aconitate hydratase, mitochondrial
Gene
ACO2
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.

Catalytic activityi

Citrate = isocitrate.

Cofactori

Binds 1 4Fe-4S cluster per subunit. Binding of a 3Fe-4S cluster leads to an inactive enzyme.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei99 – 991Substrate
Metal bindingi385 – 3851Iron-sulfur (4Fe-4S)1 Publication
Metal bindingi448 – 4481Iron-sulfur (4Fe-4S)1 Publication
Metal bindingi451 – 4511Iron-sulfur (4Fe-4S)1 Publication
Binding sitei474 – 4741Substrate
Binding sitei479 – 4791Substrate
Binding sitei607 – 6071Substrate

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. aconitate hydratase activity Source: AgBase
  3. iron ion binding Source: AgBase
Complete GO annotation...

GO - Biological processi

  1. citrate metabolic process Source: AgBase
  2. tricarboxylic acid cycle Source: AgBase
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_227707. Citric acid cycle (TCA cycle).
UniPathwayiUPA00223; UER00718.

Names & Taxonomyi

Protein namesi
Recommended name:
Aconitate hydratase, mitochondrial (EC:4.2.1.3)
Short name:
Aconitase
Alternative name(s):
Citrate hydro-lyase
Gene namesi
Name:ACO2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 5

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: AgBase
  2. nucleus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2727Mitochondrion1 Publication
Add
BLAST
Chaini28 – 780753Aconitate hydratase, mitochondrial
PRO_0000000540Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei28 – 281Pyrrolidone carboxylic acid
Modified residuei31 – 311N6-succinyllysine By similarity
Modified residuei50 – 501N6-acetyllysine; alternate By similarity
Modified residuei50 – 501N6-succinyllysine; alternate By similarity
Modified residuei138 – 1381N6-acetyllysine; alternate By similarity
Modified residuei138 – 1381N6-succinyllysine; alternate By similarity
Modified residuei144 – 1441N6-acetyllysine; alternate By similarity
Modified residuei144 – 1441N6-succinyllysine; alternate By similarity
Cross-linki144 – 144Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate By similarity
Modified residuei233 – 2331N6-acetyllysine; alternate By similarity
Modified residuei233 – 2331N6-succinyllysine; alternate By similarity
Modified residuei411 – 4111N6-succinyllysine By similarity
Modified residuei517 – 5171N6-acetyllysine; alternate By similarity
Modified residuei517 – 5171N6-succinyllysine; alternate By similarity
Modified residuei523 – 5231N6-acetyllysine; alternate By similarity
Modified residuei523 – 5231N6-succinyllysine; alternate By similarity
Modified residuei549 – 5491N6-succinyllysine By similarity
Modified residuei559 – 5591Phosphoserine By similarity
Modified residuei573 – 5731N6-acetyllysine; alternate By similarity
Modified residuei573 – 5731N6-succinyllysine; alternate By similarity
Modified residuei591 – 5911N6-succinyllysine By similarity
Modified residuei605 – 6051N6-acetyllysine; alternate By similarity
Modified residuei605 – 6051N6-succinyllysine; alternate By similarity
Modified residuei628 – 6281N6-succinyllysine By similarity
Modified residuei689 – 6891N6-succinyllysine By similarity
Modified residuei723 – 7231N6-acetyllysine; alternate By similarity
Modified residuei723 – 7231N6-succinyllysine; alternate By similarity
Modified residuei730 – 7301N6-acetyllysine; alternate By similarity
Modified residuei730 – 7301N6-succinyllysine; alternate By similarity
Modified residuei736 – 7361N6-acetyllysine By similarity
Modified residuei743 – 7431N6-acetyllysine By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Pyrrolidone carboxylic acid, Ubl conjugation

Proteomic databases

PaxDbiP20004.
PRIDEiP20004.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

IntActiP20004. 1 interaction.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi33 – 353
Beta strandi38 – 425
Helixi45 – 5915
Helixi65 – 717
Turni77 – 793
Turni84 – 863
Beta strandi88 – 914
Beta strandi94 – 996
Turni100 – 1023
Helixi103 – 11311
Beta strandi122 – 1254
Beta strandi132 – 1343
Helixi136 – 14611
Helixi148 – 16114
Beta strandi164 – 1663
Helixi173 – 1808
Beta strandi187 – 1926
Helixi195 – 2017
Beta strandi204 – 2074
Helixi210 – 2189
Beta strandi222 – 2254
Beta strandi228 – 2369
Helixi244 – 25512
Turni256 – 2627
Beta strandi263 – 2697
Helixi270 – 2745
Helixi277 – 28610
Helixi287 – 2904
Beta strandi293 – 2964
Helixi301 – 3099
Helixi313 – 3219
Helixi323 – 3253
Beta strandi335 – 3417
Helixi342 – 3443
Beta strandi348 – 3503
Beta strandi352 – 3543
Beta strandi358 – 3603
Helixi361 – 37111
Beta strandi376 – 3838
Turni384 – 3863
Helixi390 – 40415
Turni405 – 4073
Beta strandi411 – 4166
Helixi421 – 4299
Helixi432 – 4387
Beta strandi441 – 4433
Helixi449 – 4524
Beta strandi466 – 4738
Turni477 – 4815
Beta strandi486 – 4905
Helixi493 – 50210
Beta strandi504 – 5063
Turni509 – 5113
Beta strandi513 – 5153
Beta strandi521 – 5233
Turni549 – 5513
Beta strandi563 – 5653
Beta strandi579 – 58810
Helixi594 – 5974
Helixi601 – 6066
Helixi610 – 6134
Helixi614 – 6163
Turni618 – 6203
Beta strandi621 – 6233
Turni624 – 6263
Turni635 – 6373
Helixi643 – 65210
Beta strandi657 – 6604
Beta strandi663 – 6653
Helixi673 – 6808
Beta strandi683 – 6897
Helixi693 – 7019
Beta strandi705 – 7117
Helixi712 – 7176
Beta strandi723 – 7275
Helixi729 – 7313
Beta strandi738 – 7436
Beta strandi749 – 7557
Helixi760 – 7689
Helixi771 – 7788

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ACOX-ray2.05A29-779[»]
1AMIX-ray2.00A28-780[»]
1AMJX-ray2.00A28-780[»]
1C96X-ray1.81A29-779[»]
1C97X-ray1.98A29-779[»]
1FGHX-ray2.05A29-779[»]
1NISX-ray2.05A29-779[»]
1NITX-ray2.05A29-779[»]
8ACNX-ray2.00A29-780[»]
ProteinModelPortaliP20004.
SMRiP20004. Positions 29-780.

Miscellaneous databases

EvolutionaryTraceiP20004.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni192 – 1943Substrate binding
Regioni670 – 6712Substrate binding

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1048.
GeneTreeiENSGT00730000111046.
HOGENOMiHOG000224293.
HOVERGENiHBG000248.
InParanoidiP20004.
KOiK01681.
OMAiPLKCIIK.
OrthoDBiEOG74FF06.
TreeFamiTF300627.

Family and domain databases

Gene3Di3.20.19.10. 1 hit.
3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR006248. Aconitase_mito-like.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
PF00694. Aconitase_C. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF52016. SSF52016. 1 hit.
SSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR01340. aconitase_mito. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20004-1 [UniParc]FASTAAdd to Basket

« Hide

MAPYSLLVSR LQKALGARQY HVASVLCQRA KVAMSHFEPN EYIRYDLLEK    50
NINIVRKRLN RPLTLSEKIV YGHLDDPANQ EIERGKTYLR LRPDRVAMQD 100
ATAQMAMLQF ISSGLPKVAV PSTIHCDHLI EAQLGGEKDL RRAKDINQEV 150
YNFLATAGAK YGVGFWRPGS GIIHQIILEN YAYPGVLLIG TDSHTPNGGG 200
LGGICIGVGG ADAVDVMAGI PWELKCPKVI GVKLTGSLSG WTSPKDVILK 250
VAGILTVKGG TGAIVEYHGP GVDSISCTGM ATICNMGAEI GATTSVFPYN 300
HRMKKYLSKT GRADIANLAD EFKDHLVPDS GCHYDQLIEI NLSELKPHIN 350
GPFTPDLAHP VAEVGSVAEK EGWPLDIRVG LIGSCTNSSY EDMGRSAAVA 400
KQALAHGLQC KSQFTITPGS EQIRATIERD GYAQILRDVG GIVLANACGP 450
CIGQWDRKDI KKGEKNTIVT SYNRNFTGRN DANPETHAFV TSPEIVTALA 500
IAGTLKFNPE TDFLTGKDGK KFKLEAPDAD ELPRAEFDPG QDTYQHPPKD 550
SSGQQVDVSP TSQRLQLLEP FDKWDGRDLE DLQILIKVKG KCTTDHISAA 600
GPWLKFRGHL DNISNNLLIG AINVENGKAN SVRNAVTQEF GPVPDTARYY 650
KKHGIRWVVI GDENYGEGSS REHAALEPRH LGGRAIITKS FARIHETNLK 700
KQGLLPLTFA DPADYNKIHP VDKLTIKGLK DFAPGKPLTC IIKHPNGTQE 750
TILLNHTFNE TQIEWFRAGS ALNRMKELQK 780
Length:780
Mass (Da):85,359
Last modified:July 11, 2006 - v4
Checksum:i91EB21B3923F16C5
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 401N → H AA sequence 1 Publication
Sequence conflicti53 – 531N → D AA sequence 1 Publication
Sequence conflicti99 – 991Q → R in CAA90177. 1 Publication
Sequence conflicti179 – 1791E → G in AAI02643. 1 Publication
Sequence conflicti217 – 2171M → T in CAA90177. 1 Publication
Sequence conflicti409 – 4091Q → K AA sequence 1 Publication
Sequence conflicti435 – 4351I → V AA sequence 1 Publication
Sequence conflicti555 – 5551Q → R AA sequence 1 Publication
Sequence conflicti577 – 5771R → K AA sequence 1 Publication
Sequence conflicti624 – 6241V → S AA sequence 1 Publication
Sequence conflicti627 – 6271G → R AA sequence 1 Publication
Sequence conflicti652 – 6521K → Q AA sequence 1 Publication
Sequence conflicti674 – 6741A → S AA sequence 1 Publication
Sequence conflicti680 – 6801H → F AA sequence 1 Publication
Sequence conflicti727 – 7271K → Q AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49931 mRNA. Translation: CAA90177.1.
BC102642 mRNA. Translation: AAI02643.1.
PIRiS57528.
RefSeqiNP_776402.1. NM_173977.3.
UniGeneiBt.5210.

Genome annotation databases

EnsembliENSBTAT00000008431; ENSBTAP00000008431; ENSBTAG00000006429.
GeneIDi280976.
KEGGibta:280976.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49931 mRNA. Translation: CAA90177.1 .
BC102642 mRNA. Translation: AAI02643.1 .
PIRi S57528.
RefSeqi NP_776402.1. NM_173977.3.
UniGenei Bt.5210.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1ACO X-ray 2.05 A 29-779 [» ]
1AMI X-ray 2.00 A 28-780 [» ]
1AMJ X-ray 2.00 A 28-780 [» ]
1C96 X-ray 1.81 A 29-779 [» ]
1C97 X-ray 1.98 A 29-779 [» ]
1FGH X-ray 2.05 A 29-779 [» ]
1NIS X-ray 2.05 A 29-779 [» ]
1NIT X-ray 2.05 A 29-779 [» ]
8ACN X-ray 2.00 A 29-780 [» ]
ProteinModelPortali P20004.
SMRi P20004. Positions 29-780.
ModBasei Search...

Protein-protein interaction databases

IntActi P20004. 1 interaction.

Proteomic databases

PaxDbi P20004.
PRIDEi P20004.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000008431 ; ENSBTAP00000008431 ; ENSBTAG00000006429 .
GeneIDi 280976.
KEGGi bta:280976.

Organism-specific databases

CTDi 50.

Phylogenomic databases

eggNOGi COG1048.
GeneTreei ENSGT00730000111046.
HOGENOMi HOG000224293.
HOVERGENi HBG000248.
InParanoidi P20004.
KOi K01681.
OMAi PLKCIIK.
OrthoDBi EOG74FF06.
TreeFami TF300627.

Enzyme and pathway databases

UniPathwayi UPA00223 ; UER00718 .
Reactomei REACT_227707. Citric acid cycle (TCA cycle).

Miscellaneous databases

EvolutionaryTracei P20004.
NextBioi 20805079.

Family and domain databases

Gene3Di 3.20.19.10. 1 hit.
3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
InterProi IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR006248. Aconitase_mito-like.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view ]
PANTHERi PTHR11670. PTHR11670. 1 hit.
Pfami PF00330. Aconitase. 1 hit.
PF00694. Aconitase_C. 1 hit.
[Graphical view ]
PRINTSi PR00415. ACONITASE.
SUPFAMi SSF52016. SSF52016. 1 hit.
SSF53732. SSF53732. 1 hit.
TIGRFAMsi TIGR01340. aconitase_mito. 1 hit.
PROSITEi PS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Xu N., Liu W., Woldegiogis G., Sun X.
    Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Heart.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.
  3. "Identification of the reactive sulfhydryl and sequences of cysteinyl-tryptic peptides from beef heart aconitase."
    Plank D.W., Howard J.B.
    J. Biol. Chem. 263:8184-8189(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY PROTEIN SEQUENCE OF 28-780.
    Tissue: Heart.
  4. "Cysteine labeling studies of beef heart aconitase containing a 4Fe, a cubane 3Fe, or a linear 3Fe cluster."
    Plank D.W., Kennedy M.C., Beinert H., Howard J.B.
    J. Biol. Chem. 264:20385-20393(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 118-139 AND 439-457, CHARACTERIZATION OF IRON-SULFUR CLUSTERS.
    Tissue: Heart.
  5. "Crystal structures of aconitase with isocitrate and nitroisocitrate bound."
    Lauble H., Kennedy M.C., Beinert H., Stout C.D.
    Biochemistry 31:2735-2748(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 29-779 IN COMPLEX WITH IRON-SULFUR AND NITROISOCITRATE, REACTION MECHANISM.

Entry informationi

Entry nameiACON_BOVIN
AccessioniPrimary (citable) accession number: P20004
Secondary accession number(s): Q3SZZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: July 11, 2006
Last modified: September 3, 2014
This is version 131 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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