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P20004

- ACON_BOVIN

UniProt

P20004 - ACON_BOVIN

Protein

Aconitate hydratase, mitochondrial

Gene

ACO2

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 4 (11 Jul 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.

    Catalytic activityi

    Citrate = isocitrate.

    Cofactori

    Binds 1 4Fe-4S cluster per subunit. Binding of a 3Fe-4S cluster leads to an inactive enzyme.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei99 – 991Substrate
    Metal bindingi385 – 3851Iron-sulfur (4Fe-4S)2 Publications
    Metal bindingi448 – 4481Iron-sulfur (4Fe-4S)2 Publications
    Metal bindingi451 – 4511Iron-sulfur (4Fe-4S)2 Publications
    Binding sitei474 – 4741Substrate
    Binding sitei479 – 4791Substrate
    Binding sitei607 – 6071Substrate

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. aconitate hydratase activity Source: AgBase
    3. iron ion binding Source: AgBase

    GO - Biological processi

    1. citrate metabolic process Source: AgBase
    2. tricarboxylic acid cycle Source: AgBase

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_227707. Citric acid cycle (TCA cycle).
    UniPathwayiUPA00223; UER00718.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aconitate hydratase, mitochondrial (EC:4.2.1.3)
    Short name:
    Aconitase
    Alternative name(s):
    Citrate hydro-lyase
    Gene namesi
    Name:ACO2
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 5

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: AgBase
    2. nucleus Source: Ensembl

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2727MitochondrionAdd
    BLAST
    Chaini28 – 780753Aconitate hydratase, mitochondrialPRO_0000000540Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei28 – 281Pyrrolidone carboxylic acid
    Modified residuei31 – 311N6-succinyllysineBy similarity
    Modified residuei50 – 501N6-acetyllysine; alternateBy similarity
    Modified residuei50 – 501N6-succinyllysine; alternateBy similarity
    Modified residuei138 – 1381N6-acetyllysine; alternateBy similarity
    Modified residuei138 – 1381N6-succinyllysine; alternateBy similarity
    Modified residuei144 – 1441N6-acetyllysine; alternateBy similarity
    Modified residuei144 – 1441N6-succinyllysine; alternateBy similarity
    Cross-linki144 – 144Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
    Modified residuei233 – 2331N6-acetyllysine; alternateBy similarity
    Modified residuei233 – 2331N6-succinyllysine; alternateBy similarity
    Modified residuei411 – 4111N6-succinyllysineBy similarity
    Modified residuei517 – 5171N6-acetyllysine; alternateBy similarity
    Modified residuei517 – 5171N6-succinyllysine; alternateBy similarity
    Modified residuei523 – 5231N6-acetyllysine; alternateBy similarity
    Modified residuei523 – 5231N6-succinyllysine; alternateBy similarity
    Modified residuei549 – 5491N6-succinyllysineBy similarity
    Modified residuei559 – 5591PhosphoserineBy similarity
    Modified residuei573 – 5731N6-acetyllysine; alternateBy similarity
    Modified residuei573 – 5731N6-succinyllysine; alternateBy similarity
    Modified residuei591 – 5911N6-succinyllysineBy similarity
    Modified residuei605 – 6051N6-acetyllysine; alternateBy similarity
    Modified residuei605 – 6051N6-succinyllysine; alternateBy similarity
    Modified residuei628 – 6281N6-succinyllysineBy similarity
    Modified residuei689 – 6891N6-succinyllysineBy similarity
    Modified residuei723 – 7231N6-acetyllysine; alternateBy similarity
    Modified residuei723 – 7231N6-succinyllysine; alternateBy similarity
    Modified residuei730 – 7301N6-acetyllysine; alternateBy similarity
    Modified residuei730 – 7301N6-succinyllysine; alternateBy similarity
    Modified residuei736 – 7361N6-acetyllysineBy similarity
    Modified residuei743 – 7431N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Isopeptide bond, Phosphoprotein, Pyrrolidone carboxylic acid, Ubl conjugation

    Proteomic databases

    PaxDbiP20004.
    PRIDEiP20004.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    IntActiP20004. 1 interaction.

    Structurei

    Secondary structure

    1
    780
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi33 – 353
    Beta strandi38 – 425
    Helixi45 – 5915
    Helixi65 – 717
    Turni77 – 793
    Turni84 – 863
    Beta strandi88 – 914
    Beta strandi94 – 996
    Turni100 – 1023
    Helixi103 – 11311
    Beta strandi122 – 1254
    Beta strandi132 – 1343
    Helixi136 – 14611
    Helixi148 – 16114
    Beta strandi164 – 1663
    Helixi173 – 1808
    Beta strandi187 – 1926
    Helixi195 – 2017
    Beta strandi204 – 2074
    Helixi210 – 2189
    Beta strandi222 – 2254
    Beta strandi228 – 2369
    Helixi244 – 25512
    Turni256 – 2627
    Beta strandi263 – 2697
    Helixi270 – 2745
    Helixi277 – 28610
    Helixi287 – 2904
    Beta strandi293 – 2964
    Helixi301 – 3099
    Helixi313 – 3219
    Helixi323 – 3253
    Beta strandi335 – 3417
    Helixi342 – 3443
    Beta strandi348 – 3503
    Beta strandi352 – 3543
    Beta strandi358 – 3603
    Helixi361 – 37111
    Beta strandi376 – 3838
    Turni384 – 3863
    Helixi390 – 40415
    Turni405 – 4073
    Beta strandi411 – 4166
    Helixi421 – 4299
    Helixi432 – 4387
    Beta strandi441 – 4433
    Helixi449 – 4524
    Beta strandi466 – 4738
    Turni477 – 4815
    Beta strandi486 – 4905
    Helixi493 – 50210
    Beta strandi504 – 5063
    Turni509 – 5113
    Beta strandi513 – 5153
    Beta strandi521 – 5233
    Turni549 – 5513
    Beta strandi563 – 5653
    Beta strandi579 – 58810
    Helixi594 – 5974
    Helixi601 – 6066
    Helixi610 – 6134
    Helixi614 – 6163
    Turni618 – 6203
    Beta strandi621 – 6233
    Turni624 – 6263
    Turni635 – 6373
    Helixi643 – 65210
    Beta strandi657 – 6604
    Beta strandi663 – 6653
    Helixi673 – 6808
    Beta strandi683 – 6897
    Helixi693 – 7019
    Beta strandi705 – 7117
    Helixi712 – 7176
    Beta strandi723 – 7275
    Helixi729 – 7313
    Beta strandi738 – 7436
    Beta strandi749 – 7557
    Helixi760 – 7689
    Helixi771 – 7788

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ACOX-ray2.05A29-779[»]
    1AMIX-ray2.00A28-780[»]
    1AMJX-ray2.00A28-780[»]
    1C96X-ray1.81A29-779[»]
    1C97X-ray1.98A29-779[»]
    1FGHX-ray2.05A29-779[»]
    1NISX-ray2.05A29-779[»]
    1NITX-ray2.05A29-779[»]
    8ACNX-ray2.00A29-780[»]
    ProteinModelPortaliP20004.
    SMRiP20004. Positions 29-780.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP20004.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni192 – 1943Substrate binding
    Regioni670 – 6712Substrate binding

    Sequence similaritiesi

    Belongs to the aconitase/IPM isomerase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1048.
    GeneTreeiENSGT00730000111046.
    HOGENOMiHOG000224293.
    HOVERGENiHBG000248.
    InParanoidiP20004.
    KOiK01681.
    OMAiPLKCIIK.
    OrthoDBiEOG74FF06.
    TreeFamiTF300627.

    Family and domain databases

    Gene3Di3.20.19.10. 1 hit.
    3.30.499.10. 2 hits.
    3.40.1060.10. 1 hit.
    InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
    IPR015937. Acoase/IPM_deHydtase.
    IPR001030. Acoase/IPM_deHydtase_lsu_aba.
    IPR015928. Aconitase/3IPM_dehydase_swvl.
    IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
    IPR018136. Aconitase_4Fe-4S_BS.
    IPR006248. Aconitase_mito-like.
    IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
    [Graphical view]
    PANTHERiPTHR11670. PTHR11670. 1 hit.
    PfamiPF00330. Aconitase. 1 hit.
    PF00694. Aconitase_C. 1 hit.
    [Graphical view]
    PRINTSiPR00415. ACONITASE.
    SUPFAMiSSF52016. SSF52016. 1 hit.
    SSF53732. SSF53732. 1 hit.
    TIGRFAMsiTIGR01340. aconitase_mito. 1 hit.
    PROSITEiPS00450. ACONITASE_1. 1 hit.
    PS01244. ACONITASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P20004-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPYSLLVSR LQKALGARQY HVASVLCQRA KVAMSHFEPN EYIRYDLLEK    50
    NINIVRKRLN RPLTLSEKIV YGHLDDPANQ EIERGKTYLR LRPDRVAMQD 100
    ATAQMAMLQF ISSGLPKVAV PSTIHCDHLI EAQLGGEKDL RRAKDINQEV 150
    YNFLATAGAK YGVGFWRPGS GIIHQIILEN YAYPGVLLIG TDSHTPNGGG 200
    LGGICIGVGG ADAVDVMAGI PWELKCPKVI GVKLTGSLSG WTSPKDVILK 250
    VAGILTVKGG TGAIVEYHGP GVDSISCTGM ATICNMGAEI GATTSVFPYN 300
    HRMKKYLSKT GRADIANLAD EFKDHLVPDS GCHYDQLIEI NLSELKPHIN 350
    GPFTPDLAHP VAEVGSVAEK EGWPLDIRVG LIGSCTNSSY EDMGRSAAVA 400
    KQALAHGLQC KSQFTITPGS EQIRATIERD GYAQILRDVG GIVLANACGP 450
    CIGQWDRKDI KKGEKNTIVT SYNRNFTGRN DANPETHAFV TSPEIVTALA 500
    IAGTLKFNPE TDFLTGKDGK KFKLEAPDAD ELPRAEFDPG QDTYQHPPKD 550
    SSGQQVDVSP TSQRLQLLEP FDKWDGRDLE DLQILIKVKG KCTTDHISAA 600
    GPWLKFRGHL DNISNNLLIG AINVENGKAN SVRNAVTQEF GPVPDTARYY 650
    KKHGIRWVVI GDENYGEGSS REHAALEPRH LGGRAIITKS FARIHETNLK 700
    KQGLLPLTFA DPADYNKIHP VDKLTIKGLK DFAPGKPLTC IIKHPNGTQE 750
    TILLNHTFNE TQIEWFRAGS ALNRMKELQK 780
    Length:780
    Mass (Da):85,359
    Last modified:July 11, 2006 - v4
    Checksum:i91EB21B3923F16C5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti40 – 401N → H AA sequence (PubMed:3372519)Curated
    Sequence conflicti53 – 531N → D AA sequence (PubMed:3372519)Curated
    Sequence conflicti99 – 991Q → R in CAA90177. 1 PublicationCurated
    Sequence conflicti179 – 1791E → G in AAI02643. 1 PublicationCurated
    Sequence conflicti217 – 2171M → T in CAA90177. 1 PublicationCurated
    Sequence conflicti409 – 4091Q → K AA sequence (PubMed:3372519)Curated
    Sequence conflicti435 – 4351I → V AA sequence (PubMed:3372519)Curated
    Sequence conflicti555 – 5551Q → R AA sequence (PubMed:3372519)Curated
    Sequence conflicti577 – 5771R → K AA sequence (PubMed:3372519)Curated
    Sequence conflicti624 – 6241V → S AA sequence (PubMed:3372519)Curated
    Sequence conflicti627 – 6271G → R AA sequence (PubMed:3372519)Curated
    Sequence conflicti652 – 6521K → Q AA sequence (PubMed:3372519)Curated
    Sequence conflicti674 – 6741A → S AA sequence (PubMed:3372519)Curated
    Sequence conflicti680 – 6801H → F AA sequence (PubMed:3372519)Curated
    Sequence conflicti727 – 7271K → Q AA sequence (PubMed:3372519)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49931 mRNA. Translation: CAA90177.1.
    BC102642 mRNA. Translation: AAI02643.1.
    PIRiS57528.
    RefSeqiNP_776402.1. NM_173977.3.
    UniGeneiBt.5210.

    Genome annotation databases

    EnsembliENSBTAT00000008431; ENSBTAP00000008431; ENSBTAG00000006429.
    GeneIDi280976.
    KEGGibta:280976.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49931 mRNA. Translation: CAA90177.1 .
    BC102642 mRNA. Translation: AAI02643.1 .
    PIRi S57528.
    RefSeqi NP_776402.1. NM_173977.3.
    UniGenei Bt.5210.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ACO X-ray 2.05 A 29-779 [» ]
    1AMI X-ray 2.00 A 28-780 [» ]
    1AMJ X-ray 2.00 A 28-780 [» ]
    1C96 X-ray 1.81 A 29-779 [» ]
    1C97 X-ray 1.98 A 29-779 [» ]
    1FGH X-ray 2.05 A 29-779 [» ]
    1NIS X-ray 2.05 A 29-779 [» ]
    1NIT X-ray 2.05 A 29-779 [» ]
    8ACN X-ray 2.00 A 29-780 [» ]
    ProteinModelPortali P20004.
    SMRi P20004. Positions 29-780.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P20004. 1 interaction.

    Proteomic databases

    PaxDbi P20004.
    PRIDEi P20004.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000008431 ; ENSBTAP00000008431 ; ENSBTAG00000006429 .
    GeneIDi 280976.
    KEGGi bta:280976.

    Organism-specific databases

    CTDi 50.

    Phylogenomic databases

    eggNOGi COG1048.
    GeneTreei ENSGT00730000111046.
    HOGENOMi HOG000224293.
    HOVERGENi HBG000248.
    InParanoidi P20004.
    KOi K01681.
    OMAi PLKCIIK.
    OrthoDBi EOG74FF06.
    TreeFami TF300627.

    Enzyme and pathway databases

    UniPathwayi UPA00223 ; UER00718 .
    Reactomei REACT_227707. Citric acid cycle (TCA cycle).

    Miscellaneous databases

    EvolutionaryTracei P20004.
    NextBioi 20805079.

    Family and domain databases

    Gene3Di 3.20.19.10. 1 hit.
    3.30.499.10. 2 hits.
    3.40.1060.10. 1 hit.
    InterProi IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
    IPR015937. Acoase/IPM_deHydtase.
    IPR001030. Acoase/IPM_deHydtase_lsu_aba.
    IPR015928. Aconitase/3IPM_dehydase_swvl.
    IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
    IPR018136. Aconitase_4Fe-4S_BS.
    IPR006248. Aconitase_mito-like.
    IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
    [Graphical view ]
    PANTHERi PTHR11670. PTHR11670. 1 hit.
    Pfami PF00330. Aconitase. 1 hit.
    PF00694. Aconitase_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00415. ACONITASE.
    SUPFAMi SSF52016. SSF52016. 1 hit.
    SSF53732. SSF53732. 1 hit.
    TIGRFAMsi TIGR01340. aconitase_mito. 1 hit.
    PROSITEi PS00450. ACONITASE_1. 1 hit.
    PS01244. ACONITASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Xu N., Liu W., Woldegiogis G., Sun X.
      Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Heart.
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus.
      Tissue: Ileum.
    3. "Identification of the reactive sulfhydryl and sequences of cysteinyl-tryptic peptides from beef heart aconitase."
      Plank D.W., Howard J.B.
      J. Biol. Chem. 263:8184-8189(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY PROTEIN SEQUENCE OF 28-780.
      Tissue: Heart.
    4. "Cysteine labeling studies of beef heart aconitase containing a 4Fe, a cubane 3Fe, or a linear 3Fe cluster."
      Plank D.W., Kennedy M.C., Beinert H., Howard J.B.
      J. Biol. Chem. 264:20385-20393(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 118-139 AND 439-457, CHARACTERIZATION OF IRON-SULFUR CLUSTERS.
      Tissue: Heart.
    5. "Crystal structures of aconitase with isocitrate and nitroisocitrate bound."
      Lauble H., Kennedy M.C., Beinert H., Stout C.D.
      Biochemistry 31:2735-2748(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 29-779 IN COMPLEX WITH IRON-SULFUR AND THE SUBSTRATE ANALOG NITROISOCITRATE, COFACTOR, REACTION MECHANISM.

    Entry informationi

    Entry nameiACON_BOVIN
    AccessioniPrimary (citable) accession number: P20004
    Secondary accession number(s): Q3SZZ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: July 11, 2006
    Last modified: October 1, 2014
    This is version 132 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3