Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P20000 (ALDH2_BOVIN)

Last modified June 16, 2009. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Aldehyde dehydrogenase, mitochondrial
    EC=1.2.1.3
Alternative name(s):
    ALDH class 2
    ALDHI
    ALDH-E2
Gene names
Name: ALDH2
OrganismBos taurus (Bovine)
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Hide | Top

Protein attributes

Sequence length520 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Catalytic activity

An aldehyde + NAD+ + H2O = an acid + NADH.

Pathway

Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.

Subunit structure

Homotetramer.

Subcellular location

Mitochondrion matrix.

Sequence similarities

Belongs to the aldehyde dehydrogenase family.

Hide | Top

Ontologies

Keywords
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandNAD
   Molecular functionOxidoreductase
   PTMAcetylation
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentmitochondrial matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionaldehyde dehydrogenase (NAD) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2121Mitochondrion Ref.4
Chain22 – 520499Aldehyde dehydrogenase, mitochondrial
PRO_0000007167

Regions

Nucleotide binding265 – 2706NAD By similarity

Sites

Active site2881Proton acceptor
Active site3221Nucleophile
Site1891Transition state stabilizer

Amino acid modifications

Modified residue3711N6-acetyllysine By similarity

Experimental info

Sequence conflict2891L → I Ref.1
Sequence conflict2891L → I Ref.3
Sequence conflict3851A → L Ref.1
Sequence conflict3851A → L Ref.3
Sequence conflict4091V → L Ref.1
Sequence conflict4091V → L Ref.3

Secondary structure

1 ................................................................................. 520
Helix Strand Turn

Details...

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P20000-1 [UniParc].

Last modified July 1, 2008. Version 2.
Checksum: 713FC1EE8F82B2C4

FASTA52056,653
        10         20         30         40         50         60 
MLRAVALAAA RLGPRQGRRL LSAATQAVPT PNQQPEVLYN QIFINNEWHD AVSKKTFPTV 

        70         80         90        100        110        120 
NPSTGDVICH VAEGDKADVD RAVKAARAAF QLGSPWRRMD ASERGRLLNR LADLIERDRT 

       130        140        150        160        170        180 
YLAALETLDN GKPYIISYLV DLDMVLKCLR YYAGWADKYH GKTIPIDGDY FSYTRHEPVG 

       190        200        210        220        230        240 
VCGQIIPWNF PLLMQAWKLG PALATGNVVV MKVAEQTPLT ALYVANLIKE AGFPPGVVNV 

       250        260        270        280        290        300 
IPGFGPTAGA AIASHEDVDK VAFTGSTEVG HLIQVAAGKS NLKRVTLELG GKSPNIIMSD 

       310        320        330        340        350        360 
ADMDWAVEQA HFALFFNQGQ CCCAGSRTFV QEDIYAEFVE RSVARAKSRV VGNPFDSRTE 

       370        380        390        400        410        420 
QGPQVDETQF KKVLGYIKSG KEEGAKLLCG GGAAADRGYF IQPTVFGDVQ DGMTIAKEEI 

       430        440        450        460        470        480 
FGPVMQILKF KSMEEVVGRA NNSKYGLAAA VFTKDLDKAN YLSQALQAGT VWVNCYDVFG 

       490        500        510        520 
AQSPFGGYKL SGSGRELGEY GLQAYTEVKT VTVRVPQKNS

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"Sequence of the precursor of bovine liver mitochondrial aldehyde dehydrogenase as determined from its cDNA, its gene, and its functionality."
Guan K., Weiner H.
Arch. Biochem. Biophys. 277:351-360(1990) [PubMed: 1689984] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Ascending colon.
[3]"Primary structures of rat and bovine liver mitochondrial aldehyde dehydrogenases deduced from cDNA sequences."
Farres J., Guan K.-L., Weiner H.
Eur. J. Biochem. 180:67-74(1989) [PubMed: 2540003] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 92-520.
[4]"Purification and characterization of bovine brain gamma-aminobutyraldehyde dehydrogenase."
Lee J.E., Cho Y.D.
Biochem. Biophys. Res. Commun. 189:450-454(1992) [PubMed: 1449496] [Abstract]
Cited for: PROTEIN SEQUENCE OF 22-34.
Tissue: Brain.
[5]"Structure of mitochondrial aldehyde dehydrogenase: the genetic component of ethanol aversion."
Steinmetz C.G., Xie P., Weiner H., Hurley T.D.
Structure 5:701-711(1997) [PubMed: 9195888] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS).

Hide | Top

Cross-references

Sequence databases

BC116084 mRNA. Translation: AAI16085.1.
IPIIPI00705226.
PIRS09030.
RefSeqNP_001068835.1.
UniGeneBt.44041

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1A4ZX-ray2.75A/B/C/D22-520[»]
1AG8X-ray2.65A/B/C/D22-520[»]
ModBaseSearch...

Genome annotation databases

EnsemblENSBTAG00000008743. Bos taurus. [Contig view]
GeneID508629.
KEGGbta:508629.

Phylogenomic databases

HOVERGENP20000.
OMAP20000. PFDSQTE.

Enzyme and pathway databases

BRENDA1.2.1.3. 251.

Family and domain databases

InterProIPR016160. Ald_DH_CS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH.
[Graphical view]
Gene3DG3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHERPTHR11699. Aldehyde_dehyd. 1 hit.
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetSearch...

Hide | Top

Entry information

Entry nameALDH2_BOVIN
AccessionPrimary (citable) accession number: P20000
Secondary accession number(s): Q1LZC6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: July 1, 2008
Last modified: June 16, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Hide | Top

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents