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Reviewed, UniProtKB/Swiss-Prot P19994 (AMPM1_BACSU)

Last modified November 3, 2009. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Methionine aminopeptidase 1
      Short name=MAP 1
    EC=3.4.11.18
Alternative name(s):
    Peptidase M
Gene names
Name: map
Ordered Locus Names: BSU01380
OrganismBacillus subtilis [Complete proteome] [HAMAP]
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length248 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Removes the amino-terminal methionine from nascent proteins.

Catalytic activity

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.

Cofactor

Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions. Ref.4

Subcellular location

Cytoplasm. Ref.4

Induction

Expression increases gradually during the log phase of growth. Ref.4

Sequence similarities

Belongs to the peptidase M24A family.

Mass spectrometry

Molecular mass is 27409.17±0.92 Da from positions 1 - 248. Ref.4

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Ontologies

Keywords
   Cellular componentCytoplasm
   LigandCobalt
Metal-binding
   Molecular functionAminopeptidase
Hydrolase
Protease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcellular process

Inferred from electronic annotation. Source: InterPro

proteolysis

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionaminopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

cobalt ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

metalloexopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 248248Methionine aminopeptidase 1
PRO_0000148927

Sites

Metal binding941Cobalt 1 By similarity
Metal binding1051Cobalt 1 By similarity
Metal binding1051Cobalt 2 By similarity
Metal binding1681Cobalt 2 By similarity
Metal binding2011Cobalt 2 By similarity
Metal binding2321Cobalt 1 By similarity
Metal binding2321Cobalt 2 By similarity
Binding site771Substrate By similarity
Binding site1751Substrate By similarity

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Sequences

Sequence LengthMass (Da)Tools
P19994-1 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 6D6F7A593D84A761

FASTA24827,409
        10         20         30         40         50         60 
MIICKTPREL GIMREAGRIV ALTHEELKKH IKPGISTKEL DQIAERFIKK QGAIPSFKGY 

        70         80         90        100        110        120 
NGFRGSICVS VNEELVHGIP GSRVLKDGDI ISIDIGAKLN GYHGDSAWTY PVGNISDDDK 

       130        140        150        160        170        180 
KLLEVTEESL YKGLQEAKPG ERLSNISHAI QTYVENEQFS VVREYVGHGV GQDLHEDPQI 

       190        200        210        220        230        240 
PHYGPPNKGP RLKPGMVLAI EPMVNAGSRY VKTLADNWTV VTVDGKKCAH FEHTIAITET 


GFDILTRV

« Hide

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References

« Hide 'large scale' references
[1]"Cloning and characterization of a Bacillus subtilis gene homologous to E. coli secY."
Nakamura K., Nakamura A., Takamatsu H., Yoshikawa H., Yamane K.
J. Biochem. 107:603-607(1990) [PubMed: 2113521] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Genetic and transcriptional organization of the Bacillus subtilis spc-alpha region."
Suh J.-W., Boylan S.A., Oh S.H., Price C.W.
Gene 169:17-23(1996) [PubMed: 8635744] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / Marburg.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"The two authentic methionine aminopeptidase genes are differentially expressed in Bacillus subtilis."
You C., Lu H., Sekowska A., Fang G., Wang Y., Gilles A.-M., Danchin A.
BMC Microbiol. 5:57-57(2005) [PubMed: 16207374] [Abstract]
Cited for: CHARACTERIZATION, MASS SPECTROMETRY, COFACTOR, INDUCTION, SUBCELLULAR LOCATION.
Strain: 168.

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Cross-references

Sequence databases

D00619 Genomic DNA. Translation: BAA00497.1.
L47971 Genomic DNA. Translation: AAB06821.1.
AL009126 Genomic DNA. Translation: CAB11914.1.
PIRJS0493.
RefSeqNP_388019.1.

3D structure databases

HSSPHSSP built from PDB template 1O0X based on UniProtKB Q9X1I7.
ModBaseSearch...

Protein family/group databases

MEROPSM24.001.

Genome annotation databases

GeneID938929.
GenomeReviewsGene locus BSU01380 in contig AL009126_GR.
KEGGbsu:BSU01380.
NMPDRfig|224308.1.peg.138.

Organism-specific databases

SubtiListBG10447. map. [Micado]
CMRSearch...

Phylogenomic databases

HOGENOMP19994.
OMATATGPRI.

Enzyme and pathway databases

BioCycBSUB224308:BSU0138-MON.
BRENDA3.4.11.18. 150.

Family and domain databases

InterProIPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002467. Pept_M24A_MAP1.
[Graphical view]
Gene3DG3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
PANTHERPTHR10804:SF13. Pept_M24A_MAP1. 1 hit.
PTHR10804. Peptidase_M24_cat_core. 1 hit.
PfamPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSPR00599. MAPEPTIDASE.
TIGRFAMsTIGR00500. met_pdase_I. 1 hit.
PROSITEPS00680. MAP_1. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameAMPM1_BACSU
AccessionPrimary (citable) accession number: P19994
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 3, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents