Reviewed, UniProtKB/Swiss-Prot P19992 (HSD_STREX)

Names and origin

Protein names	Recommended name:     3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase     EC=1.1.1.53
Organism	Streptomyces exfoliatus (Streptomyces hydrogenans)
Taxonomic identifier	1905 [NCBI]
Taxonomic lineage	Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Androstan-3-alpha,17-beta-diol + NAD+ = 17-beta-hydroxyandrostan-3-one + NADH.
Pathway	Lipid metabolism; C21-steroid hormone metabolism.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Lipid metabolism Steroid metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-alpha(or 20-beta)-hydroxysteroid dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 255

255

3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase

PRO_0000054713

Regions

Nucleotide binding

10 – 34

25

NAD

Sites

Active site

152

1

Proton acceptor

Binding site

139

1

Substrate By similarity

Secondary structure

1

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255

Helix Strand Turn

Details...

Beta strand

7 – 12

6

Turn

13 – 15

3

Helix

17 – 28

12

Beta strand

32 – 38

7

Helix

40 – 49

10

Helix

51 – 53

3

Beta strand

54 – 58

5

Helix

64 – 78

15

Beta strand

83 – 86

4

Helix

96 – 98

3

Helix

101 – 111

11

Helix

113 – 128

16

Beta strand

132 – 137

6

Helix

140 – 142

3

Helix

150 – 170

21

Helix

171 – 173

3

Beta strand

175 – 182

8

Helix

188 – 191

4

Turn

192 – 194

3

Helix

214 – 225

12

Helix

227 – 229

3

Beta strand

236 – 240

5

Turn

241 – 245

5

Helix

249 – 254

6

Sequences

	Sequence		Length	Mass (Da)
	P19992-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 9CB93CB66AA628D5	FASTA	255	26,484
	10 20 30 40 50 60 MNDLSGKTVI ITGGARGLGA EAARQAVAAG ARVVLADVLD EEGAATAREL GDAARYQHLD 70 80 90 100 110 120 VTIEEDWQRV VAYAREEFGS VDGLVNNAGI STGMFLETES VERFRKVVDI NLTGVFIGMK 130 140 150 160 170 180 TVIPAMKDAG GGSIVNISSA AGLMGLALTS SYGASKWGVR GLSKLAAVEL GTDRIRVNSV 190 200 210 220 230 240 HPGMTYTPMT AETGIRQGEG NYPNTPMGRV GNEPGEIAGA VVKLLSDTSS YVTGAELAVD 250 GGWTTGPTVK YVMGQ

References

[1]	"Prokaryotic 20 beta-hydroxysteroid dehydrogenase is an enzyme of the 'short-chain, non-metalloenzyme' alcohol dehydrogenase type." Marekov L., Krook M., Joernvall H. FEBS Lett. 266:51-54(1990) [PubMed: 2194840] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Three-dimensional structure of holo 3 alpha,20 beta-hydroxysteroid dehydrogenase: a member of a short-chain dehydrogenase family." Ghosh D., Weeks C.M., Grochulski P., Duax W.L., Erman M., Rimsay R.L., Orr J.C. Proc. Natl. Acad. Sci. U.S.A. 88:10064-10068(1991) [PubMed: 1946424] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

S10707.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1HDC	X-ray	2.20	A/B/C/D	2-255	[»]
2HSD	X-ray	2.64	A/B/C/D	2-255	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

1.1.1.53. 97715.

Family and domain databases

InterPro

IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

PANTHER

PTHR19410. ADH_short_C2. 1 hit.

Pfam

PF00106. adh_short. 1 hit.
[Graphical view]

PRINTS

PR00081. GDHRDH.
PR00080. SDRFAMILY.

PROSITE

PS00061. ADH_SHORT. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

HSD_STREX

Accession

Primary (citable) accession number: P19992

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)