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Protein

3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase

Gene
N/A
Organism
Streptomyces exfoliatus (Streptomyces hydrogenans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Androstan-3-alpha,17-beta-diol + NAD+ = 17-beta-hydroxyandrostan-3-one + NADH.

Pathwayi: C21-steroid hormone metabolism

This protein is involved in the pathway C21-steroid hormone metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway C21-steroid hormone metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei139 – 1391SubstrateBy similarity
Active sitei152 – 1521Proton acceptor

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 3425NADAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid metabolism, Steroid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00229.

Names & Taxonomyi

Protein namesi
Recommended name:
3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase (EC:1.1.1.53)
OrganismiStreptomyces exfoliatus (Streptomyces hydrogenans)
Taxonomic identifieri1905 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3243917.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2552553-alpha-(or 20-beta)-hydroxysteroid dehydrogenasePRO_0000054713Add
BLAST

Interactioni

Subunit structurei

Homotetramer.

Structurei

Secondary structure

1
255
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 126Combined sources
Turni13 – 153Combined sources
Helixi17 – 2812Combined sources
Beta strandi32 – 387Combined sources
Helixi40 – 489Combined sources
Helixi49 – 535Combined sources
Beta strandi54 – 585Combined sources
Helixi64 – 7815Combined sources
Beta strandi83 – 864Combined sources
Helixi96 – 983Combined sources
Helixi101 – 11111Combined sources
Helixi113 – 12917Combined sources
Beta strandi132 – 1376Combined sources
Helixi140 – 1423Combined sources
Helixi150 – 17021Combined sources
Helixi171 – 1733Combined sources
Beta strandi175 – 1828Combined sources
Helixi188 – 1936Combined sources
Helixi214 – 22512Combined sources
Helixi227 – 2293Combined sources
Beta strandi236 – 2405Combined sources
Turni241 – 2455Combined sources
Helixi249 – 2535Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HDCX-ray2.20A/B/C/D2-255[»]
2HSDX-ray2.64A/B/C/D2-255[»]
ProteinModelPortaliP19992.
SMRiP19992. Positions 2-255.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP19992.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19992-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDLSGKTVI ITGGARGLGA EAARQAVAAG ARVVLADVLD EEGAATAREL
60 70 80 90 100
GDAARYQHLD VTIEEDWQRV VAYAREEFGS VDGLVNNAGI STGMFLETES
110 120 130 140 150
VERFRKVVDI NLTGVFIGMK TVIPAMKDAG GGSIVNISSA AGLMGLALTS
160 170 180 190 200
SYGASKWGVR GLSKLAAVEL GTDRIRVNSV HPGMTYTPMT AETGIRQGEG
210 220 230 240 250
NYPNTPMGRV GNEPGEIAGA VVKLLSDTSS YVTGAELAVD GGWTTGPTVK

YVMGQ
Length:255
Mass (Da):26,484
Last modified:February 1, 1991 - v1
Checksum:i9CB93CB66AA628D5
GO

Sequence databases

PIRiS10707.

Cross-referencesi

Sequence databases

PIRiS10707.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HDCX-ray2.20A/B/C/D2-255[»]
2HSDX-ray2.64A/B/C/D2-255[»]
ProteinModelPortaliP19992.
SMRiP19992. Positions 2-255.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

ChEMBLiCHEMBL3243917.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00229.

Miscellaneous databases

EvolutionaryTraceiP19992.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHSD_STREX
AccessioniPrimary (citable) accession number: P19992
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: May 11, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.