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P19976 (FRI1_SOYBN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ferritin-1, chloroplastic

EC=1.16.3.1
Alternative name(s):
SFerH-1
SOF-35
OrganismGlycine max (Soybean) (Glycine hispida) [Reference proteome]
Taxonomic identifier3847 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja

Protein attributes

Sequence length250 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.

Catalytic activity

4 Fe2+ + 4 H+ + O2 = 4 Fe3+ + 2 H2O.

Subunit structure

Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited.

Subcellular location

Plastidchloroplast. Plastid.

Tissue specificity

Leaf > hypocotyl.

Miscellaneous

Multiple cleavage sites may occur in the extension peptide yielding several smaller (26.5 kDa) ferritins subunits.

Sequence similarities

Belongs to the ferritin family.

Contains 1 ferritin-like diiron domain.

Sequence caution

The sequence AAA33958.1 differs from that shown. Reason: Contaminating sequence. Vector contamination.

The sequence AAA34016.1 differs from that shown. Reason: Contaminating sequence. Vector contamination.

Ontologies

Keywords
   Biological processIron storage
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandIron
Metal-binding
   Molecular functionOxidoreductase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcellular iron ion homeostasis

Inferred from electronic annotation. Source: UniProtKB-KW

iron ion transport

Inferred from electronic annotation. Source: InterPro

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionferric iron binding

Inferred from electronic annotation. Source: InterPro

ferroxidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4949Chloroplast Ref.3
Chain50 – 250201Ferritin-1, chloroplastic
PRO_0000008864

Regions

Domain83 – 236154Ferritin-like diiron
Region50 – 8233Extension peptide (EP)

Sites

Metal binding1001Iron 1 By similarity
Metal binding1351Iron 1 By similarity
Metal binding1351Iron 2 By similarity
Metal binding1381Iron 1 By similarity
Metal binding1841Iron 2 By similarity
Metal binding2181Iron 2 By similarity

Experimental info

Sequence conflict1611A → V in AAA33958. Ref.2
Sequence conflict1611A → V in AAA34016. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P19976 [UniParc].

Last modified December 1, 1992. Version 4.
Checksum: 3A48F00B33D7CBA9

FASTA25028,051
        10         20         30         40         50         60 
MALAPSKVST FSGFSPKPSV GGAQKNPTCS VSLSFLNEKL GSRNLRVCAS TVPLTGVIFE 

        70         80         90        100        110        120 
PFEEVKKSEL AVPTAPQVSL ARQNYADECE SAINEQINVE YNASYVYHSL FAYFDRDNVA 

       130        140        150        160        170        180 
LKGFAKFFKE SSEEEREHAE KLMKYQNTRG GRVVLHPIKN APSEFEHVEK GDALYAMELA 

       190        200        210        220        230        240 
LSLEKLVNEK LLNVHSVADR NNDPQMADFI ESEFLSEQVE SIKKISEYVA QLRRVGKGHG 

       250 
VWHFDQRLLD 

« Hide

References

[1]"Ferritin gene transcription is regulated by iron in soybean cell cultures."
Lescure A.-M., Proudhon D., Pesey H., Ragland M., Theil E.C., Briat J.-F.
Proc. Natl. Acad. Sci. U.S.A. 88:8222-8226(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Evidence for conservation of ferritin sequences among plants and animals and for a transit peptide in soybean."
Ragland M., Briat J.-F., Gagnon J., Laulhere J.-P., Massenet O., Theil E.C.
J. Biol. Chem. 265:18339-18344(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-189.
Tissue: Hypocotyl.
[3]"Purification and characterization of an iron-induced ferritin from soybean (Glycine max) cell suspensions."
Lescure A.-M., Massenet O., Briat J.-F.
Biochem. J. 272:147-150(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 50-63.
Strain: cv. Mandarin.
Tissue: Seed.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M58336 mRNA. Translation: AAA33958.1. Sequence problems.
M72894 mRNA. Translation: AAA34016.1. Sequence problems.
M64337 mRNA. Translation: AAA33959.1.
PIRA40992.
RefSeqNP_001238501.1. NM_001251572.1.
UniGeneGma.1089.

3D structure databases

ProteinModelPortalP19976.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID547824.
KEGGgmx:547824.

Phylogenomic databases

KOK00522.

Gene expression databases

GenevestigatorP19976.

Family and domain databases

Gene3D1.20.1260.10. 1 hit.
InterProIPR001519. Ferritin.
IPR009040. Ferritin-like_diiron.
IPR009078. Ferritin-like_SF.
IPR012347. Ferritin-rel.
IPR014034. Ferritin_CS.
IPR008331. Ferritin_DPS_dom.
[Graphical view]
PANTHERPTHR11431. PTHR11431. 1 hit.
PfamPF00210. Ferritin. 1 hit.
[Graphical view]
SUPFAMSSF47240. SSF47240. 1 hit.
PROSITEPS00540. FERRITIN_1. 1 hit.
PS00204. FERRITIN_2. 1 hit.
PS50905. FERRITIN_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFRI1_SOYBN
AccessionPrimary (citable) accession number: P19976
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: December 1, 1992
Last modified: June 11, 2014
This is version 104 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families