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P19973

- LSP1_MOUSE

UniProt

P19973 - LSP1_MOUSE

Protein

Lymphocyte-specific protein 1

Gene

Lsp1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    May play a role in mediating neutrophil activation and chemotaxis.1 Publication

    GO - Molecular functioni

    1. actin binding Source: MGI
    2. signal transducer activity Source: InterPro

    GO - Biological processi

    1. apoptotic process Source: MGI
    2. chemotaxis Source: MGI
    3. cytoskeleton organization Source: MGI
    4. defense response Source: MGI

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lymphocyte-specific protein 1
    Alternative name(s):
    52 kDa phosphoprotein
    Short name:
    pp52
    Lymphocyte-specific antigen WP34
    S37 protein
    Gene namesi
    Name:Lsp1
    Synonyms:Pp52, S37, Wp34
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:96832. Lsp1.

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi195 – 1951S → A: No effect on phosphorylation by PKC, PKA, MAPKAPK2 and CaMK2. 1 Publication
    Mutagenesisi243 – 2431S → A: Complete loss of phosphorylation by MAPKAPK2, partial loss of phosphorylation by PKA, no effect on phosphorylation by PKC and CaMK2. 1 Publication
    Mutagenesisi243 – 2431S → E: Complete loss of phosphorylation by MAPKAPK2, partial loss of phosphorylation by PKA, no effect on phosphorylation by PKC and CaMK2. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 330330Lymphocyte-specific protein 1PRO_0000084504Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei77 – 771Phosphoserine; by CK2Sequence Analysis
    Modified residuei78 – 781Phosphoserine; by CK2Sequence Analysis
    Modified residuei166 – 1661Phosphothreonine1 Publication
    Modified residuei168 – 1681Phosphoserine1 Publication
    Modified residuei180 – 1801PhosphoserineBy similarity
    Modified residuei243 – 2431Phosphoserine; by MAPKAPK21 Publication
    Modified residuei318 – 3181N6-acetyllysineBy similarity

    Post-translational modificationi

    Phosphorylated by casein kinase II, protein kinase C and MAPKAPK2. Phosphorylation by PKC induces translocation from membrane to cytoplasm. Phosphorylation by MAPKAPK2 may regulate neutrophil chemotaxis.4 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP19973.
    PaxDbiP19973.
    PRIDEiP19973.

    PTM databases

    PhosphoSiteiP19973.

    Expressioni

    Tissue specificityi

    Isoform 1 is expressed in normal mouse B and T-lymphocytes and in transformed B-cells but not (or in smaller amounts) in nine T-lymphoma lines tested. Isoform 2 is expressed in non-lymphoid cell lines (myocytes, stromal cells, fibroblasts).

    Gene expression databases

    ArrayExpressiP19973.
    BgeeiP19973.
    CleanExiMM_LSP1.
    GenevestigatoriP19973.

    Interactioni

    Protein-protein interaction databases

    BioGridi201211. 2 interactions.
    IntActiP19973. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP19973.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG149207.
    GeneTreeiENSGT00730000111324.
    HOVERGENiHBG001610.
    InParanoidiA2A6J6.
    KOiK14957.
    OMAiRLTAQWS.
    OrthoDBiEOG7FNC7W.
    PhylomeDBiP19973.
    TreeFamiTF336257.

    Family and domain databases

    InterProiIPR006018. Caldesmon_LSP.
    IPR002211. Lymphspecific.
    [Graphical view]
    PANTHERiPTHR18949. PTHR18949. 1 hit.
    PfamiPF02029. Caldesmon. 1 hit.
    [Graphical view]
    PRINTSiPR01083. LYMPHSPCIFIC.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P19973-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAEAAIDPRC EEQEELHAED SEGLTTQWRE EDEEEAAREQ RQRERERQLQ    50
    DQDKDKEDDG GHSLEQPGQQ TLISLKSSEL DEDEGFGDWS QKPEPRQQFW 100
    GNEGTAEGTE PSQSERPEEK QTEESSHQAK VHLEESNLSY REPDPEDAVG 150
    GSGEAEEHLI RHQVRTPSPL ALEDTVELSS PPLSPTTKLA DRTESLNRSI 200
    KKSNSVKKSQ PTLPISTIDE RLQQYTQATE SSGRTPKLSR QPSIELPSMA 250
    VASTKTLWET GEVQSQSASK TPSCQDIVAG DMSKKSLWEQ KGGSKISSTI 300
    KSTPSGKRYK FVATGHGKYE KVLVDEGSAP 330
    Length:330
    Mass (Da):36,714
    Last modified:February 1, 1996 - v2
    Checksum:iCCC27150F02859FB
    GO
    Isoform 2 (identifier: P19973-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-23: MAEAAIDPRCEEQEELHAEDSEG → MNGPALLRRNASKRGLEKLLR

    Show »
    Length:328
    Mass (Da):36,548
    Checksum:i43522E589AF1F6F0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti125 – 1273SSH → RQV in BAC27463. (PubMed:16141072)Curated
    Sequence conflicti155 – 1562AE → PK(PubMed:3263441)Curated
    Sequence conflicti158 – 1636Missing in AAH03796. (PubMed:15489334)Curated
    Sequence conflicti160 – 1601I → T(PubMed:3263441)Curated
    Sequence conflicti168 – 1681S → N(PubMed:3263441)Curated
    Sequence conflicti253 – 2531S → G(PubMed:3263441)Curated
    Sequence conflicti283 – 2831S → T(PubMed:7775393)Curated
    Sequence conflicti283 – 2831S → T(PubMed:8537319)Curated
    Isoform 2 (identifier: P19973-2)
    Sequence conflicti16 – 161L → Q in AAB37543. (PubMed:8838798)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2323MAEAA…EDSEG → MNGPALLRRNASKRGLEKLL R in isoform 2. 4 PublicationsVSP_004313Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M90316 mRNA. Translation: AAA65108.1.
    S74179 mRNA. Translation: AAB32257.1.
    M89956 mRNA. Translation: AAB48537.1.
    D49691 mRNA. Translation: BAA08541.1.
    AL603651 Genomic DNA. Translation: CAM23282.1.
    AL603651 Genomic DNA. Translation: CAM23283.1.
    BC003796 mRNA. Translation: AAH03796.1.
    U30942, U30939, U30941 Genomic DNA. Translation: AAB37542.1.
    U30942, U30940, U30941 Genomic DNA. Translation: AAB37543.1.
    AK031587 mRNA. Translation: BAC27463.1.
    CCDSiCCDS40193.1. [P19973-2]
    CCDS52450.1. [P19973-1]
    PIRiA30533.
    A46521.
    RefSeqiNP_001129543.1. NM_001136071.2. [P19973-1]
    NP_001258437.1. NM_001271508.1.
    NP_001258439.1. NM_001271510.1. [P19973-1]
    NP_062264.1. NM_019391.3. [P19973-2]
    UniGeneiMm.234003.

    Genome annotation databases

    EnsembliENSMUST00000018963; ENSMUSP00000018963; ENSMUSG00000018819. [P19973-1]
    ENSMUST00000038946; ENSMUSP00000040637; ENSMUSG00000018819. [P19973-2]
    ENSMUST00000105968; ENSMUSP00000101588; ENSMUSG00000018819. [P19973-1]
    GeneIDi16985.
    KEGGimmu:16985.
    UCSCiuc009knb.2. mouse. [P19973-1]
    uc009knf.1. mouse. [P19973-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M90316 mRNA. Translation: AAA65108.1 .
    S74179 mRNA. Translation: AAB32257.1 .
    M89956 mRNA. Translation: AAB48537.1 .
    D49691 mRNA. Translation: BAA08541.1 .
    AL603651 Genomic DNA. Translation: CAM23282.1 .
    AL603651 Genomic DNA. Translation: CAM23283.1 .
    BC003796 mRNA. Translation: AAH03796.1 .
    U30942 , U30939 , U30941 Genomic DNA. Translation: AAB37542.1 .
    U30942 , U30940 , U30941 Genomic DNA. Translation: AAB37543.1 .
    AK031587 mRNA. Translation: BAC27463.1 .
    CCDSi CCDS40193.1. [P19973-2 ]
    CCDS52450.1. [P19973-1 ]
    PIRi A30533.
    A46521.
    RefSeqi NP_001129543.1. NM_001136071.2. [P19973-1 ]
    NP_001258437.1. NM_001271508.1.
    NP_001258439.1. NM_001271510.1. [P19973-1 ]
    NP_062264.1. NM_019391.3. [P19973-2 ]
    UniGenei Mm.234003.

    3D structure databases

    ProteinModelPortali P19973.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201211. 2 interactions.
    IntActi P19973. 1 interaction.

    PTM databases

    PhosphoSitei P19973.

    Proteomic databases

    MaxQBi P19973.
    PaxDbi P19973.
    PRIDEi P19973.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000018963 ; ENSMUSP00000018963 ; ENSMUSG00000018819 . [P19973-1 ]
    ENSMUST00000038946 ; ENSMUSP00000040637 ; ENSMUSG00000018819 . [P19973-2 ]
    ENSMUST00000105968 ; ENSMUSP00000101588 ; ENSMUSG00000018819 . [P19973-1 ]
    GeneIDi 16985.
    KEGGi mmu:16985.
    UCSCi uc009knb.2. mouse. [P19973-1 ]
    uc009knf.1. mouse. [P19973-2 ]

    Organism-specific databases

    CTDi 4046.
    MGIi MGI:96832. Lsp1.

    Phylogenomic databases

    eggNOGi NOG149207.
    GeneTreei ENSGT00730000111324.
    HOVERGENi HBG001610.
    InParanoidi A2A6J6.
    KOi K14957.
    OMAi RLTAQWS.
    OrthoDBi EOG7FNC7W.
    PhylomeDBi P19973.
    TreeFami TF336257.

    Miscellaneous databases

    NextBioi 291068.
    PROi P19973.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P19973.
    Bgeei P19973.
    CleanExi MM_LSP1.
    Genevestigatori P19973.

    Family and domain databases

    InterProi IPR006018. Caldesmon_LSP.
    IPR002211. Lymphspecific.
    [Graphical view ]
    PANTHERi PTHR18949. PTHR18949. 1 hit.
    Pfami PF02029. Caldesmon. 1 hit.
    [Graphical view ]
    PRINTSi PR01083. LYMPHSPCIFIC.
    ProtoNeti Search...

    Publicationsi

    1. "A new lymphocyte-specific gene which encodes a putative Ca2+-binding protein is not expressed in transformed T lymphocyte lines."
      Jongstra J., Tidmarsh G.F., Jongstra-Bilen J., Davis M.M.
      J. Immunol. 141:3999-4004(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
      Strain: BALB/c.
    2. "Alternatively spliced pp52 mRNA in nonlymphoid stromal cells."
      Gimble J.M., Dorheim M.-A., Youkhana K., Hudson J., Nead M., Gilly M., Wood W.J. Jr., Hermanson G.G., Kuehl M., Wall R., Kincade P.W.
      J. Immunol. 150:115-121(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    3. "The LSP1 gene is expressed in cultured normal and transformed mouse macrophages."
      Jongstra J., Ittel M.E., Iscove N.N., Brady G.
      Mol. Immunol. 31:1125-1131(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: BALB/c.
    4. "Protein kinase C phosphorylates p50 LSP1 and induces translocation of p50 LSP1 in T lymphocytes."
      Matsumoto N., Kojima S., Osawa T., Toyoshima S.
      J. Biochem. 117:222-229(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PHOSPHORYLATION.
      Strain: ICR.
      Tissue: Thymus.
    5. "Lymphocyte isoforms of mouse p50 LSP1, which are phosphorylated in mitogen-activated T cells, are formed through alternative splicing and phosphorylation."
      Matsumoto N., Kita K., Kojima S., Yamamoto K., Irimura T., Miyagi M., Tsunasawa S., Toyoshima S.
      J. Biochem. 118:237-243(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING.
      Strain: ICR.
      Tissue: Thymus.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: FVB/N.
      Tissue: Mammary gland.
    8. "Alternatively spliced exons encode the tissue-specific 5' termini of leukocyte pp52 and stromal cell S37 mRNA isoforms."
      Thompson A.A., Omori S.A., Gilly M.J., May W., Gordon M.S., Wood W.J. Jr., Miyoshi E., Malone C.S., Gimble J., Denny C.T., Wall R.
      Genomics 32:352-357(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 1-124 (ISOFORMS 1 AND 2), ALTERNATIVE SPLICING.
      Strain: BALB/c.
      Tissue: Leukocyte and Stromal cell.
    9. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-127 (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Testis.
    10. "Characterization of the 50 kDa protein phosphorylated in concanavalin A-stimulated mouse T cells."
      Matsumoto N., Toyoshima S., Osawa T.
      J. Biochem. 113:630-636(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 55-81; 131-144; 211-229; 238-255 AND 311-330, PHOSPHORYLATION.
      Tissue: T-cell.
    11. "MAPKAPK2-mediated LSP1 phosphorylation and FMLP-induced neutrophil polarization."
      Wu Y., Zhan L., Ai Y., Hannigan M., Gaestel M., Huang C.-K., Madri J.A.
      Biochem. Biophys. Res. Commun. 358:170-175(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-243 BY MAPKAPK2, MUTAGENESIS OF SER-195 AND SER-243, FUNCTION.
    12. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-166 AND SER-168, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiLSP1_MOUSE
    AccessioniPrimary (citable) accession number: P19973
    Secondary accession number(s): A2A6J5
    , A2A6J6, P97339, Q04950, Q62022, Q62023, Q62024, Q8CD28, Q99L65
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 136 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3