P19973 (LSP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lymphocyte-specific protein 1

Alternative name(s):
52 kDa phosphoprotein
Short name=pp52
Lymphocyte-specific antigen WP34
S37 protein

Gene names

Name:	Lsp1
Synonyms:	Pp52, S37, Wp34

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in mediating neutrophil activation and chemotaxis. Ref.13
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Tissue specificity	Isoform 1 is expressed in normal mouse B and T-lymphocytes and in transformed B-cells but not (or in smaller amounts) in nine T-lymphoma lines tested. Isoform 2 is expressed in non-lymphoid cell lines (myocytes, stromal cells, fibroblasts).
Post-translational modification	Phosphorylated by casein kinase II, protein kinase C and MAPKAPK2. Phosphorylation by PKC induces translocation from membrane to cytoplasm. Phosphorylation by MAPKAPK2 may regulate neutrophil chemotaxis. Ref.4 Ref.10 Ref.13

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Traceable author statement PubMed 10961883. Source: MGI chemotaxis Inferred from mutant phenotype PubMed 10961883. Source: MGI cytoskeleton organization Traceable author statement PubMed 10961883. Source: MGI defense response Inferred from mutant phenotype PubMed 10961883. Source: MGI
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	actin binding Traceable author statement PubMed 10961883. Source: MGI signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P19973-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P19973-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: MAEAAIDPRCEEQEELHAEDSEG → MNGPALLRRNASKRGLEKLLR

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Lymphocyte-specific protein 1		PRO_0000084504
Amino acid modifications
	Modified residue	77	1	Phosphoserine; by CK2 Potential
	Modified residue	78	1	Phosphoserine; by CK2 Potential
	Modified residue	90	1	Phosphoserine Ref.11
	Modified residue	166	1	Phosphothreonine Ref.12 Ref.14 Ref.15
	Modified residue	168	1	Phosphoserine Ref.12 Ref.14 Ref.15
	Modified residue	180	1	Phosphoserine By similarity
	Modified residue	243	1	Phosphoserine; by MAPKAPK2 Ref.13 Ref.15
	Modified residue	318	1	N6-acetyllysine By similarity
Natural variations
	Alternative sequence	1 – 23	23	MAEAA…EDSEG → MNGPALLRRNASKRGLEKLL R in isoform 2.		VSP_004313
Experimental info
	Mutagenesis	195	1	S → A: No effect on phosphorylation by PKC, PKA, MAPKAPK2 and CaMK2. Ref.13
	Mutagenesis	243	1	S → A: Complete loss of phosphorylation by MAPKAPK2, partial loss of phosphorylation by PKA, no effect on phosphorylation by PKC and CaMK2. Ref.13
	Mutagenesis	243	1	S → E: Complete loss of phosphorylation by MAPKAPK2, partial loss of phosphorylation by PKA, no effect on phosphorylation by PKC and CaMK2. Ref.13
	Sequence conflict	125 – 127	3	SSH → RQV in BAC27463. Ref.9
	Sequence conflict	155 – 156	2	AE → PK Ref.1
	Sequence conflict	158 – 163	6	Missing in AAH03796. Ref.7
	Sequence conflict	160	1	I → T Ref.1
	Sequence conflict	168	1	S → N Ref.1
	Sequence conflict	253	1	S → G Ref.1
	Sequence conflict	283	1	S → T Ref.4
	Sequence conflict	283	1	S → T Ref.5
Isoform 2:
	Sequence conflict	16	1	L → Q in AAB37543. Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: CCC27150F02859FB	FASTA	330	36,714
	10 20 30 40 50 60 MAEAAIDPRC EEQEELHAED SEGLTTQWRE EDEEEAAREQ RQRERERQLQ DQDKDKEDDG 70 80 90 100 110 120 GHSLEQPGQQ TLISLKSSEL DEDEGFGDWS QKPEPRQQFW GNEGTAEGTE PSQSERPEEK 130 140 150 160 170 180 QTEESSHQAK VHLEESNLSY REPDPEDAVG GSGEAEEHLI RHQVRTPSPL ALEDTVELSS 190 200 210 220 230 240 PPLSPTTKLA DRTESLNRSI KKSNSVKKSQ PTLPISTIDE RLQQYTQATE SSGRTPKLSR 250 260 270 280 290 300 QPSIELPSMA VASTKTLWET GEVQSQSASK TPSCQDIVAG DMSKKSLWEQ KGGSKISSTI 310 320 330 KSTPSGKRYK FVATGHGKYE KVLVDEGSAP
	Isoform 2 [UniParc]. Checksum: 43522E589AF1F6F0	FASTA	328	36,548
	10 20 30 40 50 60 MNGPALLRRN ASKRGLEKLL RLTTQWREED EEEAAREQRQ RERERQLQDQ DKDKEDDGGH 70 80 90 100 110 120 SLEQPGQQTL ISLKSSELDE DEGFGDWSQK PEPRQQFWGN EGTAEGTEPS QSERPEEKQT 130 140 150 160 170 180 EESSHQAKVH LEESNLSYRE PDPEDAVGGS GEAEEHLIRH QVRTPSPLAL EDTVELSSPP 190 200 210 220 230 240 LSPTTKLADR TESLNRSIKK SNSVKKSQPT LPISTIDERL QQYTQATESS GRTPKLSRQP 250 260 270 280 290 300 SIELPSMAVA STKTLWETGE VQSQSASKTP SCQDIVAGDM SKKSLWEQKG GSKISSTIKS 310 320 TPSGKRYKFV ATGHGKYEKV LVDEGSAP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new lymphocyte-specific gene which encodes a putative Ca2+-binding protein is not expressed in transformed T lymphocyte lines." Jongstra J., Tidmarsh G.F., Jongstra-Bilen J., Davis M.M. J. Immunol. 141:3999-4004(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1). Strain: BALB/c.
[2]	"Alternatively spliced pp52 mRNA in nonlymphoid stromal cells." Gimble J.M., Dorheim M.-A., Youkhana K., Hudson J., Nead M., Gilly M., Wood W.J. Jr., Hermanson G.G., Kuehl M., Wall R., Kincade P.W. J. Immunol. 150:115-121(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]	"The LSP1 gene is expressed in cultured normal and transformed mouse macrophages." Jongstra J., Ittel M.E., Iscove N.N., Brady G. Mol. Immunol. 31:1125-1131(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: BALB/c.
[4]	"Protein kinase C phosphorylates p50 LSP1 and induces translocation of p50 LSP1 in T lymphocytes." Matsumoto N., Kojima S., Osawa T., Toyoshima S. J. Biochem. 117:222-229(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PHOSPHORYLATION. Strain: ICR. Tissue: Thymus.
[5]	"Lymphocyte isoforms of mouse p50 LSP1, which are phosphorylated in mitogen-activated T cells, are formed through alternative splicing and phosphorylation." Matsumoto N., Kita K., Kojima S., Yamamoto K., Irimura T., Miyagi M., Tsunasawa S., Toyoshima S. J. Biochem. 118:237-243(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING. Strain: ICR. Tissue: Thymus.
[6]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: FVB/N. Tissue: Mammary gland.
[8]	"Alternatively spliced exons encode the tissue-specific 5' termini of leukocyte pp52 and stromal cell S37 mRNA isoforms." Thompson A.A., Omori S.A., Gilly M.J., May W., Gordon M.S., Wood W.J. Jr., Miyoshi E., Malone C.S., Gimble J., Denny C.T., Wall R. Genomics 32:352-357(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 1-124 (ISOFORMS 1 AND 2), ALTERNATIVE SPLICING. Strain: BALB/c. Tissue: Leukocyte and Stromal cell.
[9]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-127 (ISOFORM 2). Strain: C57BL/6J. Tissue: Testis.
[10]	"Characterization of the 50 kDa protein phosphorylated in concanavalin A-stimulated mouse T cells." Matsumoto N., Toyoshima S., Osawa T. J. Biochem. 113:630-636(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 55-81; 131-144; 211-229; 238-255 AND 311-330, PHOSPHORYLATION. Tissue: T-cell.
[11]	"Identification of phosphoproteins and their phosphorylation sites in the WEHI-231 B lymphoma cell line." Shu H., Chen S., Bi Q., Mumby M., Brekken D.L. Mol. Cell. Proteomics 3:279-286(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-90, MASS SPECTROMETRY. Tissue: B-cell lymphoma.
[12]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-166 AND SER-168, MASS SPECTROMETRY. Tissue: Liver.
[13]	"MAPKAPK2-mediated LSP1 phosphorylation and FMLP-induced neutrophil polarization." Wu Y., Zhan L., Ai Y., Hannigan M., Gaestel M., Huang C.-K., Madri J.A. Biochem. Biophys. Res. Commun. 358:170-175(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-243 BY MAPKAPK2, MUTAGENESIS OF SER-195 AND SER-243, FUNCTION.
[14]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-166 AND SER-168, MASS SPECTROMETRY. Tissue: Melanoma.
[15]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-166; SER-168 AND SER-243, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M90316 mRNA. Translation: AAA65108.1. S74179 mRNA. Translation: AAB32257.1. M89956 mRNA. Translation: AAB48537.1. D49691 mRNA. Translation: BAA08541.1. AL603651 Genomic DNA. Translation: CAM23282.1. AL603651 Genomic DNA. Translation: CAM23283.1. BC003796 mRNA. Translation: AAH03796.1. U30942, U30939, U30941 Genomic DNA. Translation: AAB37542.1. U30942, U30940, U30941 Genomic DNA. Translation: AAB37543.1. AK031587 mRNA. Translation: BAC27463.1.
IPI	IPI00313672. IPI00331430.
PIR	A30533. A46521.
RefSeq	NP_001129543.1. NM_001136071.2. NP_001258437.1. NM_001271508.1. NP_001258439.1. NM_001271510.1. NP_062264.1. NM_019391.3.
UniGene	Mm.234003.
3D structure databases
ProteinModelPortal	P19973.
ModBase	Search...
Protein-protein interaction databases
IntAct	P19973. 1 interaction.
PTM databases
PhosphoSite	P19973.
Proteomic databases
PaxDb	P19973.
PRIDE	P19973.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000018963; ENSMUSP00000018963; ENSMUSG00000018819. ENSMUST00000038946; ENSMUSP00000040637; ENSMUSG00000018819. ENSMUST00000105968; ENSMUSP00000101588; ENSMUSG00000018819.
GeneID	16985.
KEGG	mmu:16985.
UCSC	uc009knb.2. mouse. uc009knf.1. mouse.
Organism-specific databases
CTD	4046.
MGI	MGI:96832. Lsp1.
Phylogenomic databases
eggNOG	NOG149207.
GeneTree	ENSGT00680000100117.
HOVERGEN	HBG001610.
InParanoid	A2A6J6.
KO	K14957.
OMA	RLTAQWS.
OrthoDB	EOG4NKBW0.
Gene expression databases
ArrayExpress	P19973.
Bgee	P19973.
CleanEx	MM_LSP1.
Genevestigator	P19973.
GermOnline	ENSMUSG00000018819. Mus musculus.
Family and domain databases
InterPro	IPR006018. Caldesmon_LSP. IPR002211. Lymphspecific. [Graphical view]
PANTHER	PTHR12060. PTHR12060. 1 hit.
Pfam	PF02029. Caldesmon. 1 hit. [Graphical view]
PRINTS	PR01083. LYMPHSPCIFIC.
ProtoNet	Search...
Other
NextBio	291068.
SOURCE	Search...

Entry information

Entry name

LSP1_MOUSE

Accession

Primary (citable) accession number: P19973
Secondary accession number(s): A2A6J5 , A2A6J6, P97339, Q04950, Q62022, Q62023, Q62024, Q8CD28, Q99L65

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 124 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program