Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Gamma-aminobutyric acid receptor subunit alpha-5

Gene

Gabra5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

GO - Molecular functioni

  • chloride channel activity Source: RGD
  • extracellular ligand-gated ion channel activity Source: InterPro
  • GABA-A receptor activity Source: RGD
  • GABA receptor binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

ReactomeiR-RNO-975298. Ligand-gated ion channel transport.
R-RNO-977441. GABA A receptor activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor subunit alpha-5
Alternative name(s):
GABA(A) receptor subunit alpha-5
Gene namesi
Name:Gabra5
Synonyms:Gabra-5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi61859. Gabra5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 259234ExtracellularSequence analysisAdd
BLAST
Transmembranei260 – 28122HelicalSequence analysisAdd
BLAST
Transmembranei286 – 30722HelicalSequence analysisAdd
BLAST
Transmembranei319 – 34123HelicalSequence analysisAdd
BLAST
Topological domaini342 – 42988CytoplasmicSequence analysisAdd
BLAST
Transmembranei430 – 45122HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • chloride channel complex Source: UniProtKB-KW
  • dendrite Source: RGD
  • GABA-A receptor complex Source: GO_Central
  • membrane Source: RGD
  • neuronal cell body membrane Source: RGD
  • postsynaptic membrane Source: UniProtKB-SubCell
  • receptor complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1907607.
GuidetoPHARMACOLOGYi408.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 464439Gamma-aminobutyric acid receptor subunit alpha-5PRO_0000000446Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi45 – 451N-linked (GlcNAc...)Sequence analysis
Glycosylationi145 – 1451N-linked (GlcNAc...)Sequence analysis
Disulfide bondi173 ↔ 187By similarity
Glycosylationi207 – 2071N-linked (GlcNAc...)Sequence analysis
Glycosylationi236 – 2361N-linked (GlcNAc...)Sequence analysis
Cross-linki355 – 355Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP19969.
PRIDEiP19969.

PTM databases

iPTMnetiP19969.
PhosphoSiteiP19969.

Expressioni

Gene expression databases

GenevisibleiP19969. RN.

Interactioni

Subunit structurei

Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.

GO - Molecular functioni

  • GABA receptor binding Source: RGD

Protein-protein interaction databases

IntActiP19969. 1 interaction.
MINTiMINT-1783807.
STRINGi10116.ENSRNOP00000014573.

Chemistry

BindingDBiP19969.

Structurei

3D structure databases

ProteinModelPortaliP19969.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3642. Eukaryota.
ENOG410XNQG. LUCA.
GeneTreeiENSGT00760000119010.
HOGENOMiHOG000231337.
HOVERGENiHBG051707.
InParanoidiP19969.
KOiK05175.
OMAiWDGKKAS.
OrthoDBiEOG7JX342.
PhylomeDBiP19969.
TreeFamiTF315453.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR001390. GABAAa_rcpt.
IPR005435. GABBAa5_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01079. GABAARALPHA.
PR01618. GABAARALPHA5.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19969-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNGMLSRFI MTKTLLVFCI SMTLSSHFGF SQMPTSSVQD ETNDNITIFT
60 70 80 90 100
RILDGLLDGY DNRLRPGLGE RITQVRTDIY VTSFGPVSDT EMEYTIDVFF
110 120 130 140 150
RQSWKDERLR FKGPMQRLPL NNLLASKIWT PDTFFHNGKK SIAHNMTTPN
160 170 180 190 200
KLLRLEDDGT LLYTMRLTIS AECPMQLEDF PMDAHACPLK FGSYAYPNSE
210 220 230 240 250
VVYVWTNGST KSVVVAEDGS RLNQYHLMGQ TVGTENISTS TGEYTIMTAH
260 270 280 290 300
FHLKRKIGYF VIQTYLPCIM TVILSQVSFW LNRESVPART VFGVTTVLTM
310 320 330 340 350
TTLSISARNS LPKVAYATAM DWFIAVCYAF VFSALIEFAT VNYFTKRGWA
360 370 380 390 400
WDGKKALEAA KIKKKERELI LNKSTNAFTT GKLTHPPNIP KEQLPGGTGN
410 420 430 440 450
AVGTASIRAS EEKTSESKKT YNSISKIDKM SRIVFPILFG TFNLVYWATY
460
LNREPVIKGA TSPK
Length:464
Mass (Da):52,337
Last modified:February 1, 1991 - v1
Checksum:i3BC657DDBCBA4298
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti126 – 1261S → R in CAA36248 (PubMed:2153588).Curated
Sequence conflicti168 – 1681T → M in AAC42033 (PubMed:2157817).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51992 mRNA. Translation: CAA36248.1.
L08494 Genomic DNA. Translation: AAC42033.1.
PIRiB34130.
RefSeqiNP_058991.1. NM_017295.1.
UniGeneiRn.10368.

Genome annotation databases

EnsembliENSRNOT00000014573; ENSRNOP00000014573; ENSRNOG00000010803.
ENSRNOT00000083894; ENSRNOP00000072389; ENSRNOG00000010803.
GeneIDi29707.
KEGGirno:29707.
UCSCiRGD:61859. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51992 mRNA. Translation: CAA36248.1.
L08494 Genomic DNA. Translation: AAC42033.1.
PIRiB34130.
RefSeqiNP_058991.1. NM_017295.1.
UniGeneiRn.10368.

3D structure databases

ProteinModelPortaliP19969.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP19969. 1 interaction.
MINTiMINT-1783807.
STRINGi10116.ENSRNOP00000014573.

Chemistry

BindingDBiP19969.
ChEMBLiCHEMBL1907607.
GuidetoPHARMACOLOGYi408.

PTM databases

iPTMnetiP19969.
PhosphoSiteiP19969.

Proteomic databases

PaxDbiP19969.
PRIDEiP19969.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000014573; ENSRNOP00000014573; ENSRNOG00000010803.
ENSRNOT00000083894; ENSRNOP00000072389; ENSRNOG00000010803.
GeneIDi29707.
KEGGirno:29707.
UCSCiRGD:61859. rat.

Organism-specific databases

CTDi2558.
RGDi61859. Gabra5.

Phylogenomic databases

eggNOGiKOG3642. Eukaryota.
ENOG410XNQG. LUCA.
GeneTreeiENSGT00760000119010.
HOGENOMiHOG000231337.
HOVERGENiHBG051707.
InParanoidiP19969.
KOiK05175.
OMAiWDGKKAS.
OrthoDBiEOG7JX342.
PhylomeDBiP19969.
TreeFamiTF315453.

Enzyme and pathway databases

ReactomeiR-RNO-975298. Ligand-gated ion channel transport.
R-RNO-977441. GABA A receptor activation.

Miscellaneous databases

PROiP19969.

Gene expression databases

GenevisibleiP19969. RN.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR001390. GABAAa_rcpt.
IPR005435. GABBAa5_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01079. GABAARALPHA.
PR01618. GABAARALPHA5.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Functional expression and sites of gene transcription of a novel alpha subunit of the GABAA receptor in rat brain."
    Malherbe P., Sigel E., Baur R., Persohn E., Richards J.G., Moehler H.
    FEBS Lett. 260:261-265(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Gamma-aminobutyric acidA receptor alpha 5-subunit creates novel type II benzodiazepine receptor pharmacology."
    Pritchett D.B., Seeburg P.H.
    J. Neurochem. 54:1802-1804(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiGBRA5_RAT
AccessioniPrimary (citable) accession number: P19969
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: July 6, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.