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Protein

NADH-ubiquinone oxidoreductase 21.3 kDa subunit

Gene

20H10.080

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

GO - Molecular functioni

  1. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 21.3 kDa subunit (EC:1.6.5.3, EC:1.6.99.3)
Gene namesi
ORF Names:20H10.080, NCU08930
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805 Componenti: Chromosome 2, Linkage Group V

Organism-specific databases

EuPathDBiFungiDB:NCU08930.

Subcellular locationi

  1. Mitochondrion inner membrane

  2. Note: Importation into mitochondrion is membrane potential dependent.

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 201201NADH-ubiquinone oxidoreductase 21.3 kDa subunitPRO_0000118737Add
BLAST

Interactioni

Subunit structurei

Complex I is composed of about 40 different subunits.

Protein-protein interaction databases

STRINGi5141.NCU08930.1.

Family & Domainsi

Phylogenomic databases

eggNOGiNOG68678.
HOGENOMiHOG000187575.
InParanoidiP19968.
OMAiYPCRTFV.
OrthoDBiEOG7ZGXFN.

Family and domain databases

InterProiIPR016813. NADH_Ub_cplx-1_21kDa_fun.
[Graphical view]
PIRSFiPIRSF022976. NADH_Oxi_21kDa. 1 hit.

Sequencei

Sequence statusi: Complete.

P19968-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASKVVTGVV KTTAGGVVPV SQKYTVQSVG VWERIRRAFA IDPNRSNGVP
60 70 80 90 100
LVPYNRNPSP GSLDPLAYDD PVTIPAGDIA DNPYWKRDAR RNYPRLSVVG
110 120 130 140 150
QAEAVALLSV GSASHPRVEL VGENGSKQLV AAQEAGKTGG LAKYFEGTGV
160 170 180 190 200
EAGKLVLAET GGLPPLPSGE KLGEGGKWDV YKYQLAEEPS YSEAYPCRSF

S
Length:201
Mass (Da):21,333
Last modified:June 7, 2005 - v2
Checksum:i2CA69243970E35FD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 221Q → QQ (PubMed:12655011).Curated
Sequence conflicti114 – 1141S → T in AAA33570 (PubMed:2137337).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32244 mRNA. Translation: AAA33570.1.
BX294024 Genomic DNA. Translation: CAD70991.1.
CM002240 Genomic DNA. Translation: EAA31561.1.
PIRiA34051.
RefSeqiXP_960797.1. XM_955704.2.
UniGeneiNcr.12948.

Genome annotation databases

EnsemblFungiiEFNCRT00000008129; EFNCRP00000008118; EFNCRG00000008116.
GeneIDi3876955.
KEGGincr:NCU08930.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32244 mRNA. Translation: AAA33570.1.
BX294024 Genomic DNA. Translation: CAD70991.1.
CM002240 Genomic DNA. Translation: EAA31561.1.
PIRiA34051.
RefSeqiXP_960797.1. XM_955704.2.
UniGeneiNcr.12948.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5141.NCU08930.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEFNCRT00000008129; EFNCRP00000008118; EFNCRG00000008116.
GeneIDi3876955.
KEGGincr:NCU08930.

Organism-specific databases

EuPathDBiFungiDB:NCU08930.

Phylogenomic databases

eggNOGiNOG68678.
HOGENOMiHOG000187575.
InParanoidiP19968.
OMAiYPCRTFV.
OrthoDBiEOG7ZGXFN.

Family and domain databases

InterProiIPR016813. NADH_Ub_cplx-1_21kDa_fun.
[Graphical view]
PIRSFiPIRSF022976. NADH_Oxi_21kDa. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure, in vitro expression and import into mitochondria of a 29/21-kDa subunit of complex I from Neurospora crassa."
    Videira A., Tropschug M., Werner S.
    Biochem. Biophys. Res. Commun. 166:280-285(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
    Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
    Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  3. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Entry informationi

Entry nameiNUZM_NEUCR
AccessioniPrimary (citable) accession number: P19968
Secondary accession number(s): Q7RVC1, Q871L0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: June 7, 2005
Last modified: April 29, 2015
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.