ID   CYB5R_DROME             Reviewed;         436 AA.
AC   P19967; Q9VJI2;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   21-JUN-2005, sequence version 2.
DT   24-JAN-2024, entry version 163.
DE   RecName: Full=Cytochrome b5-related protein;
DE   AltName: Full=Protein TU-36B;
GN   Name=Cyt-b5-r; ORFNames=CG13279;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2549511; DOI=10.1093/nar/17.15.6349;
RA   Levin R.J., Boychuk P.L., Croniger C.M., Kazzaz J.A., Rozek C.E.;
RT   "Structure and expression of a muscle specific gene which is adjacent to
RT   the Drosophila myosin heavy-chain gene and can encode a cytochrome b
RT   related protein.";
RL   Nucleic Acids Res. 17:6349-6367(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley; TISSUE=Head;
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA   Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA   Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC   -!- FUNCTION: May play a role in muscle cell metabolism.
CC   -!- TISSUE SPECIFICITY: Muscle.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA33113.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; X15008; CAA33113.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; AE014134; AAF53567.1; -; Genomic_DNA.
DR   EMBL; AY058287; AAL13516.1; -; mRNA.
DR   PIR; S05441; S05441.
DR   RefSeq; NP_001260514.1; NM_001273585.1.
DR   RefSeq; NP_477154.1; NM_057806.5.
DR   AlphaFoldDB; P19967; -.
DR   SMR; P19967; -.
DR   BioGRID; 61015; 4.
DR   IntAct; P19967; 1.
DR   STRING; 7227.FBpp0304264; -.
DR   PaxDb; 7227-FBpp0304264; -.
DR   DNASU; 35008; -.
DR   EnsemblMetazoa; FBtr0080932; FBpp0080486; FBgn0000406.
DR   EnsemblMetazoa; FBtr0331931; FBpp0304264; FBgn0000406.
DR   GeneID; 35008; -.
DR   KEGG; dme:Dmel_CG13279; -.
DR   AGR; FB:FBgn0000406; -.
DR   CTD; 35008; -.
DR   FlyBase; FBgn0000406; Cyt-b5-r.
DR   VEuPathDB; VectorBase:FBgn0000406; -.
DR   eggNOG; KOG4232; Eukaryota.
DR   GeneTree; ENSGT00940000176788; -.
DR   HOGENOM; CLU_627470_0_0_1; -.
DR   InParanoid; P19967; -.
DR   OMA; LMNFAAW; -.
DR   OrthoDB; 3715892at2759; -.
DR   PhylomeDB; P19967; -.
DR   SignaLink; P19967; -.
DR   BioGRID-ORCS; 35008; 0 hits in 1 CRISPR screen.
DR   ChiTaRS; Cyt-b5-r; fly.
DR   GenomeRNAi; 35008; -.
DR   PRO; PR:P19967; -.
DR   Proteomes; UP000000803; Chromosome 2L.
DR   Bgee; FBgn0000406; Expressed in head capsule and 34 other cell types or tissues.
DR   ExpressionAtlas; P19967; baseline and differential.
DR   GO; GO:0005739; C:mitochondrion; IDA:FlyBase.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR   Gene3D; 3.10.120.10; Cytochrome b5-like heme/steroid binding domain; 1.
DR   InterPro; IPR001199; Cyt_B5-like_heme/steroid-bd.
DR   InterPro; IPR036400; Cyt_B5-like_heme/steroid_sf.
DR   InterPro; IPR018506; Cyt_B5_heme-BS.
DR   InterPro; IPR005804; FA_desaturase_dom.
DR   InterPro; IPR012171; Fatty_acid_desaturase.
DR   PANTHER; PTHR16740; CYTOCHROME B5-RELATED PROTEIN-RELATED; 1.
DR   PANTHER; PTHR16740:SF1; CYTOCHROME B5-RELATED PROTEIN-RELATED; 1.
DR   Pfam; PF00173; Cyt-b5; 1.
DR   Pfam; PF00487; FA_desaturase; 1.
DR   PIRSF; PIRSF015921; FA_sphinglp_des; 1.
DR   SMART; SM01117; Cyt-b5; 1.
DR   SUPFAM; SSF55856; Cytochrome b5-like heme/steroid binding domain; 1.
DR   PROSITE; PS00191; CYTOCHROME_B5_1; 1.
DR   PROSITE; PS50255; CYTOCHROME_B5_2; 1.
DR   Genevisible; P19967; DM.
PE   2: Evidence at transcript level;
KW   Heme; Iron; Metal-binding; Reference proteome.
FT   CHAIN           1..436
FT                   /note="Cytochrome b5-related protein"
FT                   /id="PRO_0000166018"
FT   DOMAIN          16..100
FT                   /note="Cytochrome b5 heme-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00279"
FT   BINDING         59
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00279"
FT   BINDING         82
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00279"
FT   VARIANT         202
FT                   /note="A -> P"
FT   VARIANT         213
FT                   /note="I -> T"
FT   VARIANT         344
FT                   /note="R -> G"
FT   CONFLICT        219..220
FT                   /note="FD -> LN (in Ref. 1; CAA33113)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   436 AA;  50355 MW;  77C77E49C6218A02 CRC64;
     MVIEEWKKSG IATKFPTYRN SALITTHSWQ KGKRQDDGAE GLWRINDGIY DFTSFIDKHP
     GGPFWIRETK GTDITEAFEA HHLTTAPEKM IAKYKVRDAA EPRIYTLTLE EGGFYKTLKE
     RVREQLKTID KRPKKKSDLI HLGLVVSLYL LGIASAKYNS LLALVLASVA LCWTVIVSHN
     YFHRRDNWQM YAFNLGMMNF AAWRVSHALS HHIYPNSYFD LELSMFEPLL CWVPNPHIKS
     KLMRYVSWVT EPVAYALAFF IQMGTRIFYS LRHTNILYWH DLLPLTIPIA IYLGTGGSLG
     IWICVRQWLA MTSIASFSFC LIGLNAAHHD PEIYHEGDAN REDRDWGLFQ VDTIIDRGDL
     KWSQFLVLTH FGDHVLHHLF PTLDHGLLPA LYPVLYQTLD EFKGHLRECN HIEHMIGQHK
     QLLRIEPNPR APGAGK
//