Reviewed, UniProtKB/Swiss-Prot P19967 (CYB5R_DROME)

Names and origin

Protein names

Recommended name:
    Cytochrome b5-related protein
Alternative name(s):
    Protein TU-36B

Gene names

Name:	Cyt-b5-r
ORF Names:	CG13279

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role in muscle cell metabolism.
Tissue specificity	Muscle.
Sequence similarities	Contains 1 cytochrome b5 heme-binding domain.
Sequence caution	The sequence CAA33113.1 differs from that shown. Reason: Frameshift at position 348.

Ontologies

Keywords
Ligand	Heme Iron Metal-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from direct assay. Source: FlyBase
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CG17739	Q7KR42	1	EBI-142401,EBI-165118

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 436	436	Cytochrome b5-related protein		PRO_0000166018
Regions
	Domain	16 – 100	85	Cytochrome b5 heme-binding
Sites
	Metal binding	59	1	Iron (heme axial ligand) By similarity
	Metal binding	82	1	Iron (heme axial ligand) By similarity
Natural variations
	Natural variant	202	1	A → P
	Natural variant	213	1	I → T
	Natural variant	344	1	R → G
Experimental info
	Sequence conflict	219 – 220	2	FD → LN in CAA33113. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P19967-1 [UniParc]. Last modified June 21, 2005. Version 2. Checksum: 77C77E49C6218A02	FASTA	436	50,355
	10 20 30 40 50 60 MVIEEWKKSG IATKFPTYRN SALITTHSWQ KGKRQDDGAE GLWRINDGIY DFTSFIDKHP 70 80 90 100 110 120 GGPFWIRETK GTDITEAFEA HHLTTAPEKM IAKYKVRDAA EPRIYTLTLE EGGFYKTLKE 130 140 150 160 170 180 RVREQLKTID KRPKKKSDLI HLGLVVSLYL LGIASAKYNS LLALVLASVA LCWTVIVSHN 190 200 210 220 230 240 YFHRRDNWQM YAFNLGMMNF AAWRVSHALS HHIYPNSYFD LELSMFEPLL CWVPNPHIKS 250 260 270 280 290 300 KLMRYVSWVT EPVAYALAFF IQMGTRIFYS LRHTNILYWH DLLPLTIPIA IYLGTGGSLG 310 320 330 340 350 360 IWICVRQWLA MTSIASFSFC LIGLNAAHHD PEIYHEGDAN REDRDWGLFQ VDTIIDRGDL 370 380 390 400 410 420 KWSQFLVLTH FGDHVLHHLF PTLDHGLLPA LYPVLYQTLD EFKGHLRECN HIEHMIGQHK 430 QLLRIEPNPR APGAGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and expression of a muscle specific gene which is adjacent to the Drosophila myosin heavy-chain gene and can encode a cytochrome b related protein." Levin R.J., Boychuk P.L., Croniger C.M., Kazzaz J.A., Rozek C.E. Nucleic Acids Res. 17:6349-6367(1989) [PubMed: 2549511] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X15008 Genomic DNA. Translation: CAA33113.1. Frameshift. AE014134 Genomic DNA. Translation: AAF53567.1. AY058287 mRNA. Translation: AAL13516.1.
PIR	S05441.
RefSeq	NP_477154.1.
UniGene	Dm.4208
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P19967. 1 interaction.
Genome annotation databases
Ensembl	FBgn0000406. Drosophila melanogaster. [Contig view]
GeneID	35008.
KEGG	dme:Dmel_CG13279.
NMPDR	fig|7227.3.peg.2685.
Organism-specific databases
FlyBase	FBgn0000406. Cyt-b5-r.
Phylogenomic databases
HOGENOM	P19967.
OMA	P19967. GLNAAHH.
Gene expression databases
ArrayExpress	P19967.
GermOnline	CG13279. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. IPR012171. Fatty_acid/sphinglp_desaturase. IPR005804. Fatty_acid_desaturase-1. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. PF00487. FA_desaturase. 1 hit. [Graphical view]
PIRSF	PIRSF015921. FA_sphinglp_des. 1 hit.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	791390.

Entry information

Entry name

CYB5R_DROME

Accession

Primary (citable) accession number: P19967
Secondary accession number(s): Q9VJI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	June 21, 2005
Last modified:	June 16, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project