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P19957

- ELAF_HUMAN

UniProt

P19957 - ELAF_HUMAN

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Protein

Elafin

Gene

PI3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

GO - Molecular functioni

  1. endopeptidase inhibitor activity Source: ProtInc
  2. serine-type endopeptidase inhibitor activity Source: ProtInc

GO - Biological processi

  1. copulation Source: InterPro
  2. negative regulation of endopeptidase activity Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI17.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Elafin
Alternative name(s):
Elastase-specific inhibitor
Short name:
ESI
Peptidase inhibitor 3
Short name:
PI-3
Protease inhibitor WAP3
Skin-derived antileukoproteinase
Short name:
SKALP
WAP four-disulfide core domain protein 14
Gene namesi
Name:PI3
Synonyms:WAP3, WFDC14
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 20

Organism-specific databases

HGNCiHGNC:8947. PI3.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: UniProt
  2. proteinaceous extracellular matrix Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33283.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 22221 PublicationAdd
BLAST
Propeptidei23 – 60382 PublicationsPRO_0000041357Add
BLAST
Chaini61 – 11757ElafinPRO_0000041358Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi76 ↔ 105
Disulfide bondi83 ↔ 109
Disulfide bondi92 ↔ 104
Disulfide bondi98 ↔ 113

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP19957.
PaxDbiP19957.
PeptideAtlasiP19957.
PRIDEiP19957.

PTM databases

PhosphoSiteiP19957.

Miscellaneous databases

PMAP-CutDBP19957.

Expressioni

Gene expression databases

BgeeiP19957.
CleanExiHS_PI3.
GenevestigatoriP19957.

Organism-specific databases

HPAiCAB009449.
HPA017737.

Interactioni

Protein-protein interaction databases

BioGridi111284. 3 interactions.
MINTiMINT-1519171.
STRINGi9606.ENSP00000243924.

Structurei

Secondary structure

1
117
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi65 – 695
Beta strandi73 – 753
Beta strandi81 – 844
Helixi95 – 973
Beta strandi98 – 1014
Beta strandi103 – 1064
Beta strandi108 – 1147

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FLEX-ray1.90I61-117[»]
2RELNMR-A61-117[»]
ProteinModelPortaliP19957.
SMRiP19957. Positions 61-117.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP19957.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati29 – 5426SVP-1 clotting 1Add
BLAST
Repeati55 – 7218SVP-1 clotting 2Add
BLAST
Domaini69 – 11749WAPPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni29 – 72442 X tandem repeats of SVP-1 like motifAdd
BLAST

Domaini

Consists of two domains: the transglutaminase substrate domain (cementoin moiety) and the elastase inhibitor domain. The transglutaminase substrate domain serves as an anchor to localize elafin covalently to specific sites on extracellular matrix proteins.

Sequence similaritiesi

Contains 1 WAP domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG72286.
GeneTreeiENSGT00530000064218.
HOGENOMiHOG000115819.
HOVERGENiHBG018073.
InParanoidiP19957.
OMAiASERNIQ.
OrthoDBiEOG7G4QHK.
PhylomeDBiP19957.
TreeFamiTF340462.

Family and domain databases

Gene3Di4.10.75.10. 1 hit.
InterProiIPR002098. SVP_I.
IPR019541. Trappin_transglut-bd_rpt.
IPR008197. WAP.
[Graphical view]
PfamiPF10511. Cementoin. 2 hits.
PF00095. WAP. 1 hit.
[Graphical view]
PRINTSiPR00003. 4DISULPHCORE.
SMARTiSM00217. WAP. 1 hit.
[Graphical view]
SUPFAMiSSF57256. SSF57256. 1 hit.
PROSITEiPS00313. SVP_I. 2 hits.
PS51390. WAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19957-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRASSFLIVV VFLIAGTLVL EAAVTGVPVK GQDTVKGRVP FNGQDPVKGQ
60 70 80 90 100
VSVKGQDKVK AQEPVKGPVS TKPGSCPIIL IRCAMLNPPN RCLKDTDCPG
110
IKKCCEGSCG MACFVPQ
Length:117
Mass (Da):12,270
Last modified:December 1, 1992 - v3
Checksum:iA51D46257E5B03EF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti91 – 922RC → CP AA sequence (PubMed:2039600)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti17 – 171T → M.
Corresponds to variant rs17333103 [ dbSNP | Ensembl ].
VAR_052947
Natural varianti34 – 341T → P.
Corresponds to variant rs2664581 [ dbSNP | Ensembl ].
VAR_024695

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z18538 mRNA. Translation: CAA79223.1.
D13156 Genomic DNA. Translation: BAA02441.1.
S58717 Genomic DNA. Translation: AAB26371.1.
L10343 Genomic DNA. Translation: AAA36483.1.
CR542234 mRNA. Translation: CAG47030.1.
AL049767 Genomic DNA. Translation: CAB53524.1.
CH471077 Genomic DNA. Translation: EAW75873.1.
CH471077 Genomic DNA. Translation: EAW75874.1.
BC010952 mRNA. Translation: AAH10952.1.
CCDSiCCDS13344.1.
PIRiJH0614.
RefSeqiNP_002629.1. NM_002638.3.
UniGeneiHs.112341.

Genome annotation databases

EnsembliENST00000243924; ENSP00000243924; ENSG00000124102.
GeneIDi5266.
KEGGihsa:5266.
UCSCiuc002xng.3. human.

Polymorphism databases

DMDMi119262.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z18538 mRNA. Translation: CAA79223.1 .
D13156 Genomic DNA. Translation: BAA02441.1 .
S58717 Genomic DNA. Translation: AAB26371.1 .
L10343 Genomic DNA. Translation: AAA36483.1 .
CR542234 mRNA. Translation: CAG47030.1 .
AL049767 Genomic DNA. Translation: CAB53524.1 .
CH471077 Genomic DNA. Translation: EAW75873.1 .
CH471077 Genomic DNA. Translation: EAW75874.1 .
BC010952 mRNA. Translation: AAH10952.1 .
CCDSi CCDS13344.1.
PIRi JH0614.
RefSeqi NP_002629.1. NM_002638.3.
UniGenei Hs.112341.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1FLE X-ray 1.90 I 61-117 [» ]
2REL NMR - A 61-117 [» ]
ProteinModelPortali P19957.
SMRi P19957. Positions 61-117.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 111284. 3 interactions.
MINTi MINT-1519171.
STRINGi 9606.ENSP00000243924.

Protein family/group databases

MEROPSi I17.002.

PTM databases

PhosphoSitei P19957.

Polymorphism databases

DMDMi 119262.

Proteomic databases

MaxQBi P19957.
PaxDbi P19957.
PeptideAtlasi P19957.
PRIDEi P19957.

Protocols and materials databases

DNASUi 5266.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000243924 ; ENSP00000243924 ; ENSG00000124102 .
GeneIDi 5266.
KEGGi hsa:5266.
UCSCi uc002xng.3. human.

Organism-specific databases

CTDi 5266.
GeneCardsi GC20P043803.
HGNCi HGNC:8947. PI3.
HPAi CAB009449.
HPA017737.
MIMi 182257. gene.
neXtProti NX_P19957.
PharmGKBi PA33283.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG72286.
GeneTreei ENSGT00530000064218.
HOGENOMi HOG000115819.
HOVERGENi HBG018073.
InParanoidi P19957.
OMAi ASERNIQ.
OrthoDBi EOG7G4QHK.
PhylomeDBi P19957.
TreeFami TF340462.

Miscellaneous databases

EvolutionaryTracei P19957.
GeneWikii Elafin.
GenomeRNAii 5266.
NextBioi 20344.
PMAP-CutDB P19957.
PROi P19957.
SOURCEi Search...

Gene expression databases

Bgeei P19957.
CleanExi HS_PI3.
Genevestigatori P19957.

Family and domain databases

Gene3Di 4.10.75.10. 1 hit.
InterProi IPR002098. SVP_I.
IPR019541. Trappin_transglut-bd_rpt.
IPR008197. WAP.
[Graphical view ]
Pfami PF10511. Cementoin. 2 hits.
PF00095. WAP. 1 hit.
[Graphical view ]
PRINTSi PR00003. 4DISULPHCORE.
SMARTi SM00217. WAP. 1 hit.
[Graphical view ]
SUPFAMi SSF57256. SSF57256. 1 hit.
PROSITEi PS00313. SVP_I. 2 hits.
PS51390. WAP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure of the human elafin precursor preproelafin deduced from the nucleotide sequence of its gene and the presence of unique repetitive sequences in the prosegment."
    Saheki T., Ito F., Hagiwara H., Saito Y., Kuroki J., Tachibana S., Hirose S.
    Biochem. Biophys. Res. Commun. 185:240-245(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "SKALP/elafin: an elastase inhibitor from cultured human keratinocytes. Purification, cDNA sequence, and evidence for transglutaminase cross-linking."
    Molhuizen H.O.F., Alkemade H.A.C., Zeeuwen P.L.J.M., de Jongh G.J., Wieringa B., Schalkwijk J.
    J. Biol. Chem. 268:12028-12032(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 23-48 AND 85-100.
    Tissue: Keratinocyte.
  3. "Characterization and gene sequence of the precursor of elafin, an elastase-specific inhibitor in bronchial secretions."
    Sallenave J.-M., Silva A.
    Am. J. Respir. Cell Mol. Biol. 8:439-445(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Placenta.
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  8. "Elafin: an elastase-specific inhibitor of human skin. Purification, characterization, and complete amino acid sequence."
    Wiedow O., Schroeder J.-M., Gregory H., Young J.A., Christophers E.
    J. Biol. Chem. 265:14791-14795(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 61-117.
    Tissue: Stratum corneum.
  9. Erratum
    Wiedow O., Schroeder J.-M., Gregory H., Young J.A., Christophers E.
    J. Biol. Chem. 266:3356-3356(1991)
  10. "Purification and characterization of elastase-specific inhibitor. Sequence homology with mucus proteinase inhibitor."
    Sallenave J.-M., Ryle A.P.
    Biol. Chem. Hoppe-Seyler 372:13-21(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 70-94.
    Tissue: Sputum.
  11. "Isolation of elafin and elastase-specific inhibitor (ESI) from bronchial secretions. Evidence of sequence homology and immunological cross-reactivity."
    Sallenave J.-M., Marsden M.D., Ryle A.P.
    Biol. Chem. Hoppe-Seyler 373:27-33(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 61-77.
    Tissue: Sputum.
  12. "Skin-derived antileukoproteinase (SKALP), an elastase inhibitor from human keratinocytes. Purification and biochemical properties."
    Schalkwijk J., de Roo C., de Jongh G.J.
    Biochim. Biophys. Acta 1096:148-154(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 85-100.
    Tissue: Keratinocyte.
  13. "Crystal structure of an elastase-specific inhibitor elafin complexed with porcine pancreatic elastase determined at 1.9-A resolution."
    Tsunemi M., Matsuura Y., Sakakibara S., Katsube Y.
    Biochemistry 35:11570-11576(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
  14. "Solution structure of R-elafin, a specific inhibitor of elastase."
    Francart C., Dauchez M., Alix A.J.P., Lippens G.
    J. Mol. Biol. 268:666-677(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiELAF_HUMAN
AccessioniPrimary (citable) accession number: P19957
Secondary accession number(s): E1P618, Q6FG74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: December 1, 1992
Last modified: October 29, 2014
This is version 153 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3