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P19957

- ELAF_HUMAN

UniProt

P19957 - ELAF_HUMAN

Protein

Elafin

Gene

PI3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

    GO - Molecular functioni

    1. endopeptidase inhibitor activity Source: ProtInc
    2. serine-type endopeptidase inhibitor activity Source: ProtInc

    GO - Biological processi

    1. copulation Source: InterPro
    2. negative regulation of endopeptidase activity Source: GOC

    Keywords - Molecular functioni

    Protease inhibitor, Serine protease inhibitor

    Protein family/group databases

    MEROPSiI17.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Elafin
    Alternative name(s):
    Elastase-specific inhibitor
    Short name:
    ESI
    Peptidase inhibitor 3
    Short name:
    PI-3
    Protease inhibitor WAP3
    Skin-derived antileukoproteinase
    Short name:
    SKALP
    WAP four-disulfide core domain protein 14
    Gene namesi
    Name:PI3
    Synonyms:WAP3, WFDC14
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 20

    Organism-specific databases

    HGNCiHGNC:8947. PI3.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular vesicular exosome Source: UniProt
    2. proteinaceous extracellular matrix Source: ProtInc

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33283.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 22221 PublicationAdd
    BLAST
    Propeptidei23 – 60382 PublicationsPRO_0000041357Add
    BLAST
    Chaini61 – 11757ElafinPRO_0000041358Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi76 ↔ 105
    Disulfide bondi83 ↔ 109
    Disulfide bondi92 ↔ 104
    Disulfide bondi98 ↔ 113

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    MaxQBiP19957.
    PaxDbiP19957.
    PeptideAtlasiP19957.
    PRIDEiP19957.

    PTM databases

    PhosphoSiteiP19957.

    Miscellaneous databases

    PMAP-CutDBP19957.

    Expressioni

    Gene expression databases

    BgeeiP19957.
    CleanExiHS_PI3.
    GenevestigatoriP19957.

    Organism-specific databases

    HPAiCAB009449.
    HPA017737.

    Interactioni

    Protein-protein interaction databases

    BioGridi111284. 3 interactions.
    MINTiMINT-1519171.
    STRINGi9606.ENSP00000243924.

    Structurei

    Secondary structure

    1
    117
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi65 – 695
    Beta strandi73 – 753
    Beta strandi81 – 844
    Helixi95 – 973
    Beta strandi98 – 1014
    Beta strandi103 – 1064
    Beta strandi108 – 1147

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1FLEX-ray1.90I61-117[»]
    2RELNMR-A61-117[»]
    ProteinModelPortaliP19957.
    SMRiP19957. Positions 61-117.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP19957.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati29 – 5426SVP-1 clotting 1Add
    BLAST
    Repeati55 – 7218SVP-1 clotting 2Add
    BLAST
    Domaini69 – 11749WAPPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni29 – 72442 X tandem repeats of SVP-1 like motifAdd
    BLAST

    Domaini

    Consists of two domains: the transglutaminase substrate domain (cementoin moiety) and the elastase inhibitor domain. The transglutaminase substrate domain serves as an anchor to localize elafin covalently to specific sites on extracellular matrix proteins.

    Sequence similaritiesi

    Contains 1 WAP domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG72286.
    HOGENOMiHOG000115819.
    HOVERGENiHBG018073.
    InParanoidiP19957.
    OMAiASERNIQ.
    OrthoDBiEOG7G4QHK.
    PhylomeDBiP19957.
    TreeFamiTF340462.

    Family and domain databases

    Gene3Di4.10.75.10. 1 hit.
    InterProiIPR002098. SVP_I.
    IPR019541. Trappin_transglut-bd_rpt.
    IPR008197. WAP.
    [Graphical view]
    PfamiPF10511. Cementoin. 2 hits.
    PF00095. WAP. 1 hit.
    [Graphical view]
    PRINTSiPR00003. 4DISULPHCORE.
    SMARTiSM00217. WAP. 1 hit.
    [Graphical view]
    SUPFAMiSSF57256. SSF57256. 1 hit.
    PROSITEiPS00313. SVP_I. 2 hits.
    PS51390. WAP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P19957-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRASSFLIVV VFLIAGTLVL EAAVTGVPVK GQDTVKGRVP FNGQDPVKGQ    50
    VSVKGQDKVK AQEPVKGPVS TKPGSCPIIL IRCAMLNPPN RCLKDTDCPG 100
    IKKCCEGSCG MACFVPQ 117
    Length:117
    Mass (Da):12,270
    Last modified:December 1, 1992 - v3
    Checksum:iA51D46257E5B03EF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti91 – 922RC → CP AA sequence (PubMed:2039600)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti17 – 171T → M.
    Corresponds to variant rs17333103 [ dbSNP | Ensembl ].
    VAR_052947
    Natural varianti34 – 341T → P.
    Corresponds to variant rs2664581 [ dbSNP | Ensembl ].
    VAR_024695

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z18538 mRNA. Translation: CAA79223.1.
    D13156 Genomic DNA. Translation: BAA02441.1.
    S58717 Genomic DNA. Translation: AAB26371.1.
    L10343 Genomic DNA. Translation: AAA36483.1.
    CR542234 mRNA. Translation: CAG47030.1.
    AL049767 Genomic DNA. Translation: CAB53524.1.
    CH471077 Genomic DNA. Translation: EAW75873.1.
    CH471077 Genomic DNA. Translation: EAW75874.1.
    BC010952 mRNA. Translation: AAH10952.1.
    CCDSiCCDS13344.1.
    PIRiJH0614.
    RefSeqiNP_002629.1. NM_002638.3.
    UniGeneiHs.112341.

    Genome annotation databases

    EnsembliENST00000243924; ENSP00000243924; ENSG00000124102.
    GeneIDi5266.
    KEGGihsa:5266.
    UCSCiuc002xng.3. human.

    Polymorphism databases

    DMDMi119262.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z18538 mRNA. Translation: CAA79223.1 .
    D13156 Genomic DNA. Translation: BAA02441.1 .
    S58717 Genomic DNA. Translation: AAB26371.1 .
    L10343 Genomic DNA. Translation: AAA36483.1 .
    CR542234 mRNA. Translation: CAG47030.1 .
    AL049767 Genomic DNA. Translation: CAB53524.1 .
    CH471077 Genomic DNA. Translation: EAW75873.1 .
    CH471077 Genomic DNA. Translation: EAW75874.1 .
    BC010952 mRNA. Translation: AAH10952.1 .
    CCDSi CCDS13344.1.
    PIRi JH0614.
    RefSeqi NP_002629.1. NM_002638.3.
    UniGenei Hs.112341.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1FLE X-ray 1.90 I 61-117 [» ]
    2REL NMR - A 61-117 [» ]
    ProteinModelPortali P19957.
    SMRi P19957. Positions 61-117.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111284. 3 interactions.
    MINTi MINT-1519171.
    STRINGi 9606.ENSP00000243924.

    Protein family/group databases

    MEROPSi I17.002.

    PTM databases

    PhosphoSitei P19957.

    Polymorphism databases

    DMDMi 119262.

    Proteomic databases

    MaxQBi P19957.
    PaxDbi P19957.
    PeptideAtlasi P19957.
    PRIDEi P19957.

    Protocols and materials databases

    DNASUi 5266.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000243924 ; ENSP00000243924 ; ENSG00000124102 .
    GeneIDi 5266.
    KEGGi hsa:5266.
    UCSCi uc002xng.3. human.

    Organism-specific databases

    CTDi 5266.
    GeneCardsi GC20P043803.
    HGNCi HGNC:8947. PI3.
    HPAi CAB009449.
    HPA017737.
    MIMi 182257. gene.
    neXtProti NX_P19957.
    PharmGKBi PA33283.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG72286.
    HOGENOMi HOG000115819.
    HOVERGENi HBG018073.
    InParanoidi P19957.
    OMAi ASERNIQ.
    OrthoDBi EOG7G4QHK.
    PhylomeDBi P19957.
    TreeFami TF340462.

    Miscellaneous databases

    EvolutionaryTracei P19957.
    GeneWikii Elafin.
    GenomeRNAii 5266.
    NextBioi 20344.
    PMAP-CutDB P19957.
    PROi P19957.
    SOURCEi Search...

    Gene expression databases

    Bgeei P19957.
    CleanExi HS_PI3.
    Genevestigatori P19957.

    Family and domain databases

    Gene3Di 4.10.75.10. 1 hit.
    InterProi IPR002098. SVP_I.
    IPR019541. Trappin_transglut-bd_rpt.
    IPR008197. WAP.
    [Graphical view ]
    Pfami PF10511. Cementoin. 2 hits.
    PF00095. WAP. 1 hit.
    [Graphical view ]
    PRINTSi PR00003. 4DISULPHCORE.
    SMARTi SM00217. WAP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57256. SSF57256. 1 hit.
    PROSITEi PS00313. SVP_I. 2 hits.
    PS51390. WAP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of the human elafin precursor preproelafin deduced from the nucleotide sequence of its gene and the presence of unique repetitive sequences in the prosegment."
      Saheki T., Ito F., Hagiwara H., Saito Y., Kuroki J., Tachibana S., Hirose S.
      Biochem. Biophys. Res. Commun. 185:240-245(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "SKALP/elafin: an elastase inhibitor from cultured human keratinocytes. Purification, cDNA sequence, and evidence for transglutaminase cross-linking."
      Molhuizen H.O.F., Alkemade H.A.C., Zeeuwen P.L.J.M., de Jongh G.J., Wieringa B., Schalkwijk J.
      J. Biol. Chem. 268:12028-12032(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 23-48 AND 85-100.
      Tissue: Keratinocyte.
    3. "Characterization and gene sequence of the precursor of elafin, an elastase-specific inhibitor in bronchial secretions."
      Sallenave J.-M., Silva A.
      Am. J. Respir. Cell Mol. Biol. 8:439-445(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Placenta.
    4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "The DNA sequence and comparative analysis of human chromosome 20."
      Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
      , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
      Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    8. "Elafin: an elastase-specific inhibitor of human skin. Purification, characterization, and complete amino acid sequence."
      Wiedow O., Schroeder J.-M., Gregory H., Young J.A., Christophers E.
      J. Biol. Chem. 265:14791-14795(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 61-117.
      Tissue: Stratum corneum.
    9. Erratum
      Wiedow O., Schroeder J.-M., Gregory H., Young J.A., Christophers E.
      J. Biol. Chem. 266:3356-3356(1991)
    10. "Purification and characterization of elastase-specific inhibitor. Sequence homology with mucus proteinase inhibitor."
      Sallenave J.-M., Ryle A.P.
      Biol. Chem. Hoppe-Seyler 372:13-21(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 70-94.
      Tissue: Sputum.
    11. "Isolation of elafin and elastase-specific inhibitor (ESI) from bronchial secretions. Evidence of sequence homology and immunological cross-reactivity."
      Sallenave J.-M., Marsden M.D., Ryle A.P.
      Biol. Chem. Hoppe-Seyler 373:27-33(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 61-77.
      Tissue: Sputum.
    12. "Skin-derived antileukoproteinase (SKALP), an elastase inhibitor from human keratinocytes. Purification and biochemical properties."
      Schalkwijk J., de Roo C., de Jongh G.J.
      Biochim. Biophys. Acta 1096:148-154(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 85-100.
      Tissue: Keratinocyte.
    13. "Crystal structure of an elastase-specific inhibitor elafin complexed with porcine pancreatic elastase determined at 1.9-A resolution."
      Tsunemi M., Matsuura Y., Sakakibara S., Katsube Y.
      Biochemistry 35:11570-11576(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
    14. "Solution structure of R-elafin, a specific inhibitor of elastase."
      Francart C., Dauchez M., Alix A.J.P., Lippens G.
      J. Mol. Biol. 268:666-677(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.

    Entry informationi

    Entry nameiELAF_HUMAN
    AccessioniPrimary (citable) accession number: P19957
    Secondary accession number(s): E1P618, Q6FG74
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 152 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 20
      Human chromosome 20: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3