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P19957 (ELAF_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 147. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Elafin
Alternative name(s):
Elastase-specific inhibitor
Short name=ESI
Peptidase inhibitor 3
Short name=PI-3
Protease inhibitor WAP3
Skin-derived antileukoproteinase
Short name=SKALP
WAP four-disulfide core domain protein 14
Gene names
Name:PI3
Synonyms:WAP3, WFDC14
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length117 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

Subcellular location

Secreted.

Domain

Consists of two domains: the transglutaminase substrate domain (cementoin moiety) and the elastase inhibitor domain. The transglutaminase substrate domain serves as an anchor to localize elafin covalently to specific sites on extracellular matrix proteins.

Sequence similarities

Contains 1 WAP domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Ref.2
Propeptide23 – 6038
PRO_0000041357
Chain61 – 11757Elafin
PRO_0000041358

Regions

Repeat29 – 5426SVP-1 clotting 1
Repeat55 – 7218SVP-1 clotting 2
Domain69 – 11749WAP
Region29 – 72442 X tandem repeats of SVP-1 like motif

Amino acid modifications

Disulfide bond76 ↔ 105
Disulfide bond83 ↔ 109
Disulfide bond92 ↔ 104
Disulfide bond98 ↔ 113

Natural variations

Natural variant171T → M.
Corresponds to variant rs17333103 [ dbSNP | Ensembl ].
VAR_052947
Natural variant341T → P.
Corresponds to variant rs2664581 [ dbSNP | Ensembl ].
VAR_024695

Experimental info

Sequence conflict91 – 922RC → CP AA sequence Ref.10

Secondary structure

.............. 117
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P19957 [UniParc].

Last modified December 1, 1992. Version 3.
Checksum: A51D46257E5B03EF

FASTA11712,270
        10         20         30         40         50         60 
MRASSFLIVV VFLIAGTLVL EAAVTGVPVK GQDTVKGRVP FNGQDPVKGQ VSVKGQDKVK 

        70         80         90        100        110 
AQEPVKGPVS TKPGSCPIIL IRCAMLNPPN RCLKDTDCPG IKKCCEGSCG MACFVPQ 

« Hide

References

« Hide 'large scale' references
[1]"Primary structure of the human elafin precursor preproelafin deduced from the nucleotide sequence of its gene and the presence of unique repetitive sequences in the prosegment."
Saheki T., Ito F., Hagiwara H., Saito Y., Kuroki J., Tachibana S., Hirose S.
Biochem. Biophys. Res. Commun. 185:240-245(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"SKALP/elafin: an elastase inhibitor from cultured human keratinocytes. Purification, cDNA sequence, and evidence for transglutaminase cross-linking."
Molhuizen H.O.F., Alkemade H.A.C., Zeeuwen P.L.J.M., de Jongh G.J., Wieringa B., Schalkwijk J.
J. Biol. Chem. 268:12028-12032(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 23-48 AND 85-100.
Tissue: Keratinocyte.
[3]"Characterization and gene sequence of the precursor of elafin, an elastase-specific inhibitor in bronchial secretions."
Sallenave J.-M., Silva A.
Am. J. Respir. Cell Mol. Biol. 8:439-445(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Placenta.
[4]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. expand/collapse author list , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[8]"Elafin: an elastase-specific inhibitor of human skin. Purification, characterization, and complete amino acid sequence."
Wiedow O., Schroeder J.-M., Gregory H., Young J.A., Christophers E.
J. Biol. Chem. 265:14791-14795(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 61-117.
Tissue: Stratum corneum.
[9]Erratum
Wiedow O., Schroeder J.-M., Gregory H., Young J.A., Christophers E.
J. Biol. Chem. 266:3356-3356(1991)
[10]"Purification and characterization of elastase-specific inhibitor. Sequence homology with mucus proteinase inhibitor."
Sallenave J.-M., Ryle A.P.
Biol. Chem. Hoppe-Seyler 372:13-21(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 70-94.
Tissue: Sputum.
[11]"Isolation of elafin and elastase-specific inhibitor (ESI) from bronchial secretions. Evidence of sequence homology and immunological cross-reactivity."
Sallenave J.-M., Marsden M.D., Ryle A.P.
Biol. Chem. Hoppe-Seyler 373:27-33(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 61-77.
Tissue: Sputum.
[12]"Skin-derived antileukoproteinase (SKALP), an elastase inhibitor from human keratinocytes. Purification and biochemical properties."
Schalkwijk J., de Roo C., de Jongh G.J.
Biochim. Biophys. Acta 1096:148-154(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 85-100.
Tissue: Keratinocyte.
[13]"Crystal structure of an elastase-specific inhibitor elafin complexed with porcine pancreatic elastase determined at 1.9-A resolution."
Tsunemi M., Matsuura Y., Sakakibara S., Katsube Y.
Biochemistry 35:11570-11576(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
[14]"Solution structure of R-elafin, a specific inhibitor of elastase."
Francart C., Dauchez M., Alix A.J.P., Lippens G.
J. Mol. Biol. 268:666-677(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z18538 mRNA. Translation: CAA79223.1.
D13156 Genomic DNA. Translation: BAA02441.1.
S58717 Genomic DNA. Translation: AAB26371.1.
L10343 Genomic DNA. Translation: AAA36483.1.
CR542234 mRNA. Translation: CAG47030.1.
AL049767 Genomic DNA. Translation: CAB53524.1.
CH471077 Genomic DNA. Translation: EAW75873.1.
CH471077 Genomic DNA. Translation: EAW75874.1.
BC010952 mRNA. Translation: AAH10952.1.
PIRJH0614.
RefSeqNP_002629.1. NM_002638.3.
UniGeneHs.112341.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1FLEX-ray1.90I61-117[»]
2RELNMR-A61-117[»]
ProteinModelPortalP19957.
SMRP19957. Positions 61-117.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111284. 3 interactions.
MINTMINT-1519171.
STRING9606.ENSP00000243924.

Protein family/group databases

MEROPSI17.002.

PTM databases

PhosphoSiteP19957.

Polymorphism databases

DMDM119262.

Proteomic databases

PaxDbP19957.
PeptideAtlasP19957.
PRIDEP19957.

Protocols and materials databases

DNASU5266.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000243924; ENSP00000243924; ENSG00000124102.
GeneID5266.
KEGGhsa:5266.
UCSCuc002xng.3. human.

Organism-specific databases

CTD5266.
GeneCardsGC20P043803.
HGNCHGNC:8947. PI3.
HPACAB009449.
HPA017737.
MIM182257. gene.
neXtProtNX_P19957.
PharmGKBPA33283.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG72286.
HOGENOMHOG000115819.
HOVERGENHBG018073.
InParanoidP19957.
OMAGSCPNIL.
OrthoDBEOG7G4QHK.
PhylomeDBP19957.
TreeFamTF340462.

Gene expression databases

BgeeP19957.
CleanExHS_PI3.
GenevestigatorP19957.

Family and domain databases

Gene3D4.10.75.10. 1 hit.
InterProIPR002098. SVP_I.
IPR019541. Trappin_transglut-bd_rpt.
IPR008197. WAP-type_4-diS_core.
[Graphical view]
PfamPF10511. Cementoin. 2 hits.
PF00095. WAP. 1 hit.
[Graphical view]
PRINTSPR00003. 4DISULPHCORE.
SMARTSM00217. WAP. 1 hit.
[Graphical view]
SUPFAMSSF57256. SSF57256. 1 hit.
PROSITEPS00313. SVP_I. 2 hits.
PS51390. WAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP19957.
GeneWikiElafin.
GenomeRNAi5266.
NextBio20344.
PMAP-CutDBP19957.
PROP19957.
SOURCESearch...

Entry information

Entry nameELAF_HUMAN
AccessionPrimary (citable) accession number: P19957
Secondary accession number(s): E1P618, Q6FG74
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: December 1, 1992
Last modified: April 16, 2014
This is version 147 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 20

Human chromosome 20: entries, gene names and cross-references to MIM