Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein TolA

Gene

tolA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the TonB-independent uptake of group A colicins (colicins A, E1, E2, E3, and K). Necessary for the colicins to reach their respective targets after initial binding to the bacteria. Also involved in the translocation of bacteriophage DNA.

GO - Molecular functioni

GO - Biological processi

  • bacteriocin transport Source: EcoliWiki
  • cell division Source: EcoCyc
  • cellular response to bacteriocin Source: EcoCyc
  • protein import Source: EcoliWiki
  • viral entry into host cell Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Bacteriocin transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11007-MONOMER.
ECOL316407:JW0729-MONOMER.

Protein family/group databases

TCDBi2.C.1.2.1. the tonb-exbb-exbd/tola-tolq-tolr (tonb) family of auxiliary proteins for energization of outer membrane receptor (omr)-mediated active transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein TolA
Gene namesi
Name:tolA
Synonyms:cim, excC, lky
Ordered Locus Names:b0739, JW0729
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11007. tolA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1313CytoplasmicSequence analysisAdd
BLAST
Transmembranei14 – 3421HelicalSequence analysisAdd
BLAST
Topological domaini35 – 421387PeriplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell division site Source: EcoCyc
  • integral component of membrane Source: EcoliWiki
  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 421421Protein TolAPRO_0000072622Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi363 ↔ 388

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP19934.
PRIDEiP19934.

Interactioni

Subunit structurei

Interacts, via domain II, with porins OmpC, PhoE and LamB.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
caaP044806EBI-1120026,EBI-6559916From a different organism.
tolBP0A8555EBI-1120026,EBI-7180728

Protein-protein interaction databases

BioGridi4261827. 171 interactions.
DIPiDIP-11005N.
IntActiP19934. 8 interactions.
MINTiMINT-7888479.
STRINGi511145.b0739.

Structurei

Secondary structure

1
421
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi327 – 3293Combined sources
Beta strandi331 – 3344Combined sources
Helixi335 – 34915Combined sources
Turni350 – 3523Combined sources
Helixi355 – 3584Combined sources
Beta strandi363 – 3697Combined sources
Turni371 – 3733Combined sources
Beta strandi375 – 3839Combined sources
Helixi385 – 39713Combined sources
Helixi406 – 4127Combined sources
Beta strandi416 – 4205Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1S62NMR-A325-421[»]
1TOLX-ray1.85A295-421[»]
3QDPX-ray2.15A302-421[»]
3QDRX-ray2.65A302-421[»]
ProteinModelPortaliP19934.
SMRiP19934. Positions 333-421.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP19934.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati224 – 22961
Repeati230 – 23452
Repeati235 – 24063
Repeati241 – 24554
Repeati246 – 25055
Repeati251 – 25556
Repeati256 – 26057
Repeati261 – 26668
Repeati267 – 27159
Repeati272 – 277610
Repeati278 – 282511
Repeati283 – 287512
Repeati288 – 292513

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni48 – 310263Domain II (alpha-helical)Add
BLAST
Regioni224 – 2926913 X tandem repeats of [EDA]-K(1,2)-A(2,4)Add
BLAST
Regioni311 – 421111Domain III (functional)Add
BLAST

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108Z82. Bacteria.
COG3064. LUCA.
HOGENOMiHOG000283070.
KOiK03646.

Family and domain databases

InterProiIPR014161. Tol-Pal_TolA.
[Graphical view]
PfamiPF06519. TolA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02794. tolA_full. 2 hits.

Sequencei

Sequence statusi: Complete.

P19934-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKATEQNDK LKRAIIISAV LHVILFAALI WSSFDENIEA SAGGGGGSSI
60 70 80 90 100
DAVMVDSGAV VEQYKRMQSQ ESSAKRSDEQ RKMKEQQAAE ELREKQAAEQ
110 120 130 140 150
ERLKQLEKER LAAQEQKKQA EEAAKQAELK QKQAEEAAAK AAADAKAKAE
160 170 180 190 200
ADAKAAEEAA KKAAADAKKK AEAEAAKAAA EAQKKAEAAA AALKKKAEAA
210 220 230 240 250
EAAAAEARKK AATEAAEKAK AEAEKKAAAE KAAADKKAAA EKAAADKKAA
260 270 280 290 300
EKAAAEKAAA DKKAAAEKAA ADKKAAAAKA AAEKAAAAKA AAEADDIFGE
310 320 330 340 350
LSSGKNAPKT GGGAKGNNAS PAGSGNTKNN GASGADINNY AGQIKSAIES
360 370 380 390 400
KFYDASSYAG KTCTLRIKLA PDGMLLDIKP EGGDPALCQA ALAAAKLAKI
410 420
PKPPSQAVYE VFKNAPLDFK P
Length:421
Mass (Da):43,157
Last modified:February 1, 1991 - v1
Checksum:i8B2F52B4B97C655E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28232 Genomic DNA. Translation: AAA24683.1.
U00096 Genomic DNA. Translation: AAC73833.1.
AP009048 Genomic DNA. Translation: BAA35405.1.
PIRiJV0057.
RefSeqiNP_415267.1. NC_000913.3.
WP_000030637.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73833; AAC73833; b0739.
BAA35405; BAA35405; BAA35405.
GeneIDi946625.
KEGGiecj:JW0729.
eco:b0739.
PATRICi32116677. VBIEscCol129921_0771.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28232 Genomic DNA. Translation: AAA24683.1.
U00096 Genomic DNA. Translation: AAC73833.1.
AP009048 Genomic DNA. Translation: BAA35405.1.
PIRiJV0057.
RefSeqiNP_415267.1. NC_000913.3.
WP_000030637.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1S62NMR-A325-421[»]
1TOLX-ray1.85A295-421[»]
3QDPX-ray2.15A302-421[»]
3QDRX-ray2.65A302-421[»]
ProteinModelPortaliP19934.
SMRiP19934. Positions 333-421.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261827. 171 interactions.
DIPiDIP-11005N.
IntActiP19934. 8 interactions.
MINTiMINT-7888479.
STRINGi511145.b0739.

Protein family/group databases

TCDBi2.C.1.2.1. the tonb-exbb-exbd/tola-tolq-tolr (tonb) family of auxiliary proteins for energization of outer membrane receptor (omr)-mediated active transport.

Proteomic databases

PaxDbiP19934.
PRIDEiP19934.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73833; AAC73833; b0739.
BAA35405; BAA35405; BAA35405.
GeneIDi946625.
KEGGiecj:JW0729.
eco:b0739.
PATRICi32116677. VBIEscCol129921_0771.

Organism-specific databases

EchoBASEiEB1000.
EcoGeneiEG11007. tolA.

Phylogenomic databases

eggNOGiENOG4108Z82. Bacteria.
COG3064. LUCA.
HOGENOMiHOG000283070.
KOiK03646.

Enzyme and pathway databases

BioCyciEcoCyc:EG11007-MONOMER.
ECOL316407:JW0729-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP19934.
PROiP19934.

Family and domain databases

InterProiIPR014161. Tol-Pal_TolA.
[Graphical view]
PfamiPF06519. TolA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02794. tolA_full. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequences of the tolA and tolB genes and localization of their products, components of a multistep translocation system in Escherichia coli."
    Levengood S.K., Webster R.E.
    J. Bacteriol. 171:6600-6609(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / JM105 / ATCC 47016.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "TolA: a membrane protein involved in colicin uptake contains an extended helical region."
    Levengood S.K., Beyer W.F. Jr., Webster R.E.
    Proc. Natl. Acad. Sci. U.S.A. 88:5939-5943(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAINS.
  6. "TolA central domain interacts with Escherichia coli porins."
    Derouiche R., Gavioli M., Benedetti H., Prilipov A., Lazdunski C., Lloubes R.
    EMBO J. 15:6408-6415(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PORINS.
  7. "Filamentous phage infection: crystal structure of g3p in complex with its coreceptor, the C-terminal domain of TolA."
    Lubkowski J., Hennecke F., Plueckthun A., Wlodawer A.
    Structure 7:711-722(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 298-421.

Entry informationi

Entry nameiTOLA_ECOLI
AccessioniPrimary (citable) accession number: P19934
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: March 16, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.