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Protein

Tol-Pal system protein TolA

Gene

tolA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity (PubMed:1683466, PubMed:8416897, PubMed:11115123, PubMed:11994151, PubMed:17233825). Is also involved in the uptake of group A colicins (colicins A, E1, E2, E3, and K) and in the uptake of filamentous phage DNA (PubMed:1683466). The Tol-Pal system is also required for polar localization of chemoreceptors clusters (PubMed:24720726).1 Publication6 Publications

GO - Molecular functioni

  • disordered domain specific binding Source: CAFA
  • protein domain specific binding Source: CAFA
  • transporter activity Source: InterPro
  • virion binding Source: CAFA

GO - Biological processi

  • bacteriocin transport Source: EcoliWiki
  • cell cycle Source: UniProtKB-KW
  • cell division Source: EcoCyc
  • cellular response to bacteriocin Source: EcoCyc
  • protein import Source: EcoliWiki
  • viral entry into host cell Source: EcoCyc

Keywordsi

Biological processCell cycle, Cell division

Enzyme and pathway databases

BioCyciEcoCyc:EG11007-MONOMER

Protein family/group databases

TCDBi2.C.1.2.1 the tonb-exbb-exbd/tola-tolq-tolr outer membrane receptor energizers and stabilizers (tonb/tola) family

Names & Taxonomyi

Protein namesi
Recommended name:
Tol-Pal system protein TolACurated
Gene namesi
Name:tolA1 Publication
Synonyms:cim, excC, lky
Ordered Locus Names:b0739, JW0729
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11007 tolA

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 13Cytoplasmic1 PublicationAdd BLAST13
Transmembranei14 – 34HelicalSequence analysisAdd BLAST21
Topological domaini35 – 421Periplasmic1 PublicationAdd BLAST387

GO - Cellular componenti

  • cell division site Source: EcoCyc
  • integral component of membrane Source: EcoliWiki
  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoliWiki

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutants lacking the tol-pal cluster suffer delayed outer membrane invagination and contain large outer membrane blebs at constriction sites and cell poles.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000726221 – 421Tol-Pal system protein TolAAdd BLAST421

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi363 ↔ 388Combined sources

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP19934
PRIDEiP19934

Interactioni

Subunit structurei

The Tol-Pal system is composed of five core proteins: the inner membrane proteins TolA, TolQ and TolR, the periplasmic protein TolB and the outer membrane protein Pal. They form a network linking the inner and outer membranes and the peptidoglycan layer (PubMed:17233825). TolA interacts with TolQ and TolR via its N-terminal domain (PubMed:7744737, PubMed:10419942). Interacts with CpoB, and with the trimeric porins OmpC, OmpF, PhoE and LamB via its central domain (PubMed:8978668, PubMed:11994151). Interacts with TolB via its C-terminal domain (PubMed:11994151). Also interacts with Pal via its C-terminal domain. This interaction is proton motive force dependent and requires TolQ and TolR (PubMed:11115123, PubMed:11722743).1 Publication6 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • disordered domain specific binding Source: CAFA
  • protein domain specific binding Source: CAFA

Protein-protein interaction databases

BioGridi4261827, 179 interactors
DIPiDIP-11005N
IntActiP19934, 9 interactors
MINTiP19934
STRINGi316385.ECDH10B_0806

Structurei

Secondary structure

1421
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi327 – 329Combined sources3
Beta strandi331 – 334Combined sources4
Helixi335 – 349Combined sources15
Turni350 – 352Combined sources3
Helixi355 – 358Combined sources4
Beta strandi363 – 369Combined sources7
Turni371 – 373Combined sources3
Beta strandi375 – 383Combined sources9
Helixi385 – 397Combined sources13
Helixi406 – 412Combined sources7
Beta strandi416 – 420Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S62NMR-A325-421[»]
1TOLX-ray1.85A295-421[»]
3QDPX-ray2.15A302-421[»]
3QDRX-ray2.65A302-421[»]
ProteinModelPortaliP19934
SMRiP19934
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP19934

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati224 – 22916
Repeati230 – 23425
Repeati235 – 24036
Repeati241 – 24545
Repeati246 – 25055
Repeati251 – 25565
Repeati256 – 26075
Repeati261 – 26686
Repeati267 – 27195
Repeati272 – 277106
Repeati278 – 282115
Repeati283 – 287125
Repeati288 – 292135

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni48 – 310Domain II (alpha-helical)Add BLAST263
Regioni224 – 29213 X tandem repeats of [EDA]-K(1,2)-A(2,4)Add BLAST69
Regioni311 – 421Domain III (functional)Add BLAST111

Domaini

Contains an N-terminal domain that anchors the protein to the inner membrane, a central domain (domain II), and a C-terminal domain (domain III), which is the functional portion of the protein (PubMed:2068069). The C-terminal domain is involved in interaction with TolB and Pal, and is also involved in direct interaction with colicins and phages. It adapts its conformation upon binding to various partners (PubMed:11115123, PubMed:11994151, PubMed:15701516).4 Publications

Sequence similaritiesi

Belongs to the TolA family.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108Z82 Bacteria
COG3064 LUCA
HOGENOMiHOG000283070
KOiK03646
OMAiNLFRDIS

Family and domain databases

InterProiView protein in InterPro
IPR014161 Tol-Pal_TolA
PfamiView protein in Pfam
PF06519 TolA, 1 hit
TIGRFAMsiTIGR02794 tolA_full, 2 hits

Sequencei

Sequence statusi: Complete.

P19934-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKATEQNDK LKRAIIISAV LHVILFAALI WSSFDENIEA SAGGGGGSSI
60 70 80 90 100
DAVMVDSGAV VEQYKRMQSQ ESSAKRSDEQ RKMKEQQAAE ELREKQAAEQ
110 120 130 140 150
ERLKQLEKER LAAQEQKKQA EEAAKQAELK QKQAEEAAAK AAADAKAKAE
160 170 180 190 200
ADAKAAEEAA KKAAADAKKK AEAEAAKAAA EAQKKAEAAA AALKKKAEAA
210 220 230 240 250
EAAAAEARKK AATEAAEKAK AEAEKKAAAE KAAADKKAAA EKAAADKKAA
260 270 280 290 300
EKAAAEKAAA DKKAAAEKAA ADKKAAAAKA AAEKAAAAKA AAEADDIFGE
310 320 330 340 350
LSSGKNAPKT GGGAKGNNAS PAGSGNTKNN GASGADINNY AGQIKSAIES
360 370 380 390 400
KFYDASSYAG KTCTLRIKLA PDGMLLDIKP EGGDPALCQA ALAAAKLAKI
410 420
PKPPSQAVYE VFKNAPLDFK P
Length:421
Mass (Da):43,157
Last modified:February 1, 1991 - v1
Checksum:i8B2F52B4B97C655E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28232 Genomic DNA Translation: AAA24683.1
U00096 Genomic DNA Translation: AAC73833.1
AP009048 Genomic DNA Translation: BAA35405.1
PIRiJV0057
RefSeqiNP_415267.1, NC_000913.3
WP_000030637.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73833; AAC73833; b0739
BAA35405; BAA35405; BAA35405
GeneIDi946625
KEGGiecj:JW0729
eco:b0739
PATRICifig|511145.12.peg.771

Similar proteinsi

Entry informationi

Entry nameiTOLA_ECOLI
AccessioniPrimary (citable) accession number: P19934
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: April 25, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health