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Protein

Protein TolA

Gene

tolA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the TonB-independent uptake of group A colicins (colicins A, E1, E2, E3, and K). Necessary for the colicins to reach their respective targets after initial binding to the bacteria. Also involved in the translocation of bacteriophage DNA.

GO - Molecular functioni

  • disordered domain specific binding Source: CAFA
  • protein domain specific binding Source: CAFA
  • transporter activity Source: InterPro
  • virion binding Source: CAFA

GO - Biological processi

  • bacteriocin transport Source: EcoliWiki
  • cell division Source: EcoCyc
  • cellular response to bacteriocin Source: EcoCyc
  • protein import Source: EcoliWiki
  • viral entry into host cell Source: EcoCyc

Keywordsi

Biological processBacteriocin transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11007-MONOMER.

Protein family/group databases

TCDBi2.C.1.2.1. the tonb-exbb-exbd/tola-tolq-tolr outer membrane receptor energizers and stabilizers (tonb/tola) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein TolA
Gene namesi
Name:tolA
Synonyms:cim, excC, lky
Ordered Locus Names:b0739, JW0729
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11007. tolA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 13CytoplasmicSequence analysisAdd BLAST13
Transmembranei14 – 34HelicalSequence analysisAdd BLAST21
Topological domaini35 – 421PeriplasmicSequence analysisAdd BLAST387

GO - Cellular componenti

  • cell division site Source: EcoCyc
  • integral component of membrane Source: EcoliWiki
  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoliWiki

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000726221 – 421Protein TolAAdd BLAST421

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi363 ↔ 388

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP19934.
PRIDEiP19934.

Interactioni

Subunit structurei

Interacts, via domain II, with porins OmpC, PhoE and LamB.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • disordered domain specific binding Source: CAFA
  • protein domain specific binding Source: CAFA

Protein-protein interaction databases

BioGridi4261827. 171 interactors.
DIPiDIP-11005N.
IntActiP19934. 8 interactors.
MINTiMINT-7888479.
STRINGi511145.b0739.

Structurei

Secondary structure

1421
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi327 – 329Combined sources3
Beta strandi331 – 334Combined sources4
Helixi335 – 349Combined sources15
Turni350 – 352Combined sources3
Helixi355 – 358Combined sources4
Beta strandi363 – 369Combined sources7
Turni371 – 373Combined sources3
Beta strandi375 – 383Combined sources9
Helixi385 – 397Combined sources13
Helixi406 – 412Combined sources7
Beta strandi416 – 420Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S62NMR-A325-421[»]
1TOLX-ray1.85A295-421[»]
3QDPX-ray2.15A302-421[»]
3QDRX-ray2.65A302-421[»]
ProteinModelPortaliP19934.
SMRiP19934.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP19934.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati224 – 22916
Repeati230 – 23425
Repeati235 – 24036
Repeati241 – 24545
Repeati246 – 25055
Repeati251 – 25565
Repeati256 – 26075
Repeati261 – 26686
Repeati267 – 27195
Repeati272 – 277106
Repeati278 – 282115
Repeati283 – 287125
Repeati288 – 292135

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni48 – 310Domain II (alpha-helical)Add BLAST263
Regioni224 – 29213 X tandem repeats of [EDA]-K(1,2)-A(2,4)Add BLAST69
Regioni311 – 421Domain III (functional)Add BLAST111

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108Z82. Bacteria.
COG3064. LUCA.
HOGENOMiHOG000283070.
KOiK03646.

Family and domain databases

InterProiView protein in InterPro
IPR014161. Tol-Pal_TolA.
PfamiView protein in Pfam
PF06519. TolA. 1 hit.
TIGRFAMsiTIGR02794. tolA_full. 2 hits.

Sequencei

Sequence statusi: Complete.

P19934-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKATEQNDK LKRAIIISAV LHVILFAALI WSSFDENIEA SAGGGGGSSI
60 70 80 90 100
DAVMVDSGAV VEQYKRMQSQ ESSAKRSDEQ RKMKEQQAAE ELREKQAAEQ
110 120 130 140 150
ERLKQLEKER LAAQEQKKQA EEAAKQAELK QKQAEEAAAK AAADAKAKAE
160 170 180 190 200
ADAKAAEEAA KKAAADAKKK AEAEAAKAAA EAQKKAEAAA AALKKKAEAA
210 220 230 240 250
EAAAAEARKK AATEAAEKAK AEAEKKAAAE KAAADKKAAA EKAAADKKAA
260 270 280 290 300
EKAAAEKAAA DKKAAAEKAA ADKKAAAAKA AAEKAAAAKA AAEADDIFGE
310 320 330 340 350
LSSGKNAPKT GGGAKGNNAS PAGSGNTKNN GASGADINNY AGQIKSAIES
360 370 380 390 400
KFYDASSYAG KTCTLRIKLA PDGMLLDIKP EGGDPALCQA ALAAAKLAKI
410 420
PKPPSQAVYE VFKNAPLDFK P
Length:421
Mass (Da):43,157
Last modified:February 1, 1991 - v1
Checksum:i8B2F52B4B97C655E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28232 Genomic DNA. Translation: AAA24683.1.
U00096 Genomic DNA. Translation: AAC73833.1.
AP009048 Genomic DNA. Translation: BAA35405.1.
PIRiJV0057.
RefSeqiNP_415267.1. NC_000913.3.
WP_000030637.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73833; AAC73833; b0739.
BAA35405; BAA35405; BAA35405.
GeneIDi946625.
KEGGiecj:JW0729.
eco:b0739.
PATRICi32116677. VBIEscCol129921_0771.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28232 Genomic DNA. Translation: AAA24683.1.
U00096 Genomic DNA. Translation: AAC73833.1.
AP009048 Genomic DNA. Translation: BAA35405.1.
PIRiJV0057.
RefSeqiNP_415267.1. NC_000913.3.
WP_000030637.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S62NMR-A325-421[»]
1TOLX-ray1.85A295-421[»]
3QDPX-ray2.15A302-421[»]
3QDRX-ray2.65A302-421[»]
ProteinModelPortaliP19934.
SMRiP19934.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261827. 171 interactors.
DIPiDIP-11005N.
IntActiP19934. 8 interactors.
MINTiMINT-7888479.
STRINGi511145.b0739.

Protein family/group databases

TCDBi2.C.1.2.1. the tonb-exbb-exbd/tola-tolq-tolr outer membrane receptor energizers and stabilizers (tonb/tola) family.

Proteomic databases

PaxDbiP19934.
PRIDEiP19934.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73833; AAC73833; b0739.
BAA35405; BAA35405; BAA35405.
GeneIDi946625.
KEGGiecj:JW0729.
eco:b0739.
PATRICi32116677. VBIEscCol129921_0771.

Organism-specific databases

EchoBASEiEB1000.
EcoGeneiEG11007. tolA.

Phylogenomic databases

eggNOGiENOG4108Z82. Bacteria.
COG3064. LUCA.
HOGENOMiHOG000283070.
KOiK03646.

Enzyme and pathway databases

BioCyciEcoCyc:EG11007-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP19934.
PROiPR:P19934.

Family and domain databases

InterProiView protein in InterPro
IPR014161. Tol-Pal_TolA.
PfamiView protein in Pfam
PF06519. TolA. 1 hit.
TIGRFAMsiTIGR02794. tolA_full. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiTOLA_ECOLI
AccessioniPrimary (citable) accession number: P19934
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: May 10, 2017
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.