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Protein

Carbon monoxide dehydrogenase large chain

Gene

coxL

Organism
Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of carbon monoxide to carbon dioxide.2 Publications

Catalytic activityi

CO + a quinone + H2O = CO2 + a quinol.2 Publications

Cofactori

Protein has several cofactor binding sites:

Kineticsi

  1. KM=16.4 µM for 1,4-benzoquinone1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi388Copper1 Publication1
    Metal bindingi763Molybdenum2 Publications1

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Copper, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-19674.
    BRENDAi1.2.99.2. 4399.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carbon monoxide dehydrogenase large chain (EC:1.2.5.32 Publications)
    Short name:
    CO dehydrogenase subunit L
    Short name:
    CO-DH L
    Gene namesi
    Name:coxL
    Ordered Locus Names:OCA5_pHCG300310
    Encoded oniPlasmid megaplasmid pHCG30 Publication
    OrganismiOligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5)
    Taxonomic identifieri504832 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeOligotropha
    Proteomesi
    • UP000007730 Componenti: Plasmid pHCG3

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000798101 – 809Carbon monoxide dehydrogenase large chainAdd BLAST809

    Proteomic databases

    PRIDEiP19919.

    Interactioni

    Subunit structurei

    Dimer of heterotrimers. Each heterotrimer consists of a large, a medium and a small subunit.1 Publication

    Structurei

    Secondary structure

    1809
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi11 – 18Combined sources8
    Helixi30 – 33Combined sources4
    Turni34 – 36Combined sources3
    Helixi41 – 43Combined sources3
    Beta strandi50 – 56Combined sources7
    Beta strandi60 – 68Combined sources9
    Helixi70 – 74Combined sources5
    Beta strandi78 – 83Combined sources6
    Helixi84 – 87Combined sources4
    Helixi88 – 90Combined sources3
    Beta strandi93 – 96Combined sources4
    Beta strandi100 – 106Combined sources7
    Beta strandi108 – 111Combined sources4
    Beta strandi117 – 125Combined sources9
    Helixi126 – 135Combined sources10
    Beta strandi137 – 142Combined sources6
    Helixi149 – 151Combined sources3
    Helixi162 – 164Combined sources3
    Beta strandi180 – 187Combined sources8
    Helixi189 – 198Combined sources10
    Beta strandi200 – 209Combined sources10
    Beta strandi221 – 227Combined sources7
    Turni228 – 231Combined sources4
    Beta strandi232 – 237Combined sources6
    Helixi242 – 253Combined sources12
    Helixi257 – 259Combined sources3
    Beta strandi260 – 263Combined sources4
    Turni271 – 274Combined sources4
    Helixi279 – 291Combined sources13
    Beta strandi295 – 298Combined sources4
    Helixi301 – 307Combined sources7
    Beta strandi314 – 322Combined sources9
    Beta strandi328 – 338Combined sources11
    Turni352 – 355Combined sources4
    Helixi356 – 358Combined sources3
    Turni359 – 362Combined sources4
    Beta strandi368 – 375Combined sources8
    Beta strandi382 – 384Combined sources3
    Turni389 – 391Combined sources3
    Helixi392 – 410Combined sources19
    Helixi414 – 421Combined sources8
    Helixi425 – 427Combined sources3
    Beta strandi429 – 431Combined sources3
    Helixi443 – 454Combined sources12
    Helixi456 – 471Combined sources16
    Beta strandi476 – 487Combined sources12
    Turni495 – 497Combined sources3
    Beta strandi507 – 513Combined sources7
    Beta strandi519 – 524Combined sources6
    Beta strandi528 – 530Combined sources3
    Helixi532 – 544Combined sources13
    Helixi548 – 550Combined sources3
    Beta strandi551 – 554Combined sources4
    Turni558 – 560Combined sources3
    Turni572 – 575Combined sources4
    Helixi576 – 599Combined sources24
    Helixi603 – 605Combined sources3
    Beta strandi606 – 608Combined sources3
    Beta strandi610 – 615Combined sources6
    Beta strandi618 – 624Combined sources7
    Helixi625 – 634Combined sources10
    Beta strandi642 – 650Combined sources9
    Beta strandi659 – 669Combined sources11
    Turni670 – 672Combined sources3
    Beta strandi675 – 685Combined sources11
    Helixi692 – 711Combined sources20
    Beta strandi723 – 725Combined sources3
    Turni728 – 730Combined sources3
    Turni736 – 738Combined sources3
    Beta strandi743 – 746Combined sources4
    Helixi764 – 782Combined sources19
    Turni783 – 785Combined sources3
    Helixi795 – 804Combined sources10
    Turni805 – 808Combined sources4

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1N5WX-ray1.50B/E1-809[»]
    1N60X-ray1.19B/E1-809[»]
    1N61X-ray1.30B/E1-809[»]
    1N62X-ray1.09B/E1-809[»]
    1N63X-ray1.21B/E1-809[»]
    1ZXIX-ray1.70B/E1-809[»]
    ProteinModelPortaliP19919.
    SMRiP19919.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP19919.

    Family & Domainsi

    Phylogenomic databases

    KOiK03520.
    OMAiPYPEDYI.

    Family and domain databases

    Gene3Di3.30.365.10. 6 hits.
    3.90.1170.50. 1 hit.
    InterProiIPR000674. Ald_Oxase/Xan_DH_a/b.
    IPR008274. AldOxase/xan_DH_Mopterin-bd.
    IPR012780. CO_Mo_DH_lsu.
    [Graphical view]
    PfamiPF01315. Ald_Xan_dh_C. 1 hit.
    PF02738. Ald_Xan_dh_C2. 1 hit.
    [Graphical view]
    SMARTiSM01008. Ald_Xan_dh_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF54665. SSF54665. 1 hit.
    SSF56003. SSF56003. 1 hit.
    TIGRFAMsiTIGR02416. CO_dehy_Mo_lg. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P19919-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MNIQTTVEPT SAERAEKLQG MGCKRKRVED IRFTQGKGNY VDDVKLPGML
    60 70 80 90 100
    FGDFVRSSHA HARIKSIDTS KAKALPGVFA VLTAADLKPL NLHYMPTLAG
    110 120 130 140 150
    DVQAVLADEK VLFQNQEVAF VVAKDRYVAA DAIELVEVDY EPLPVLVDPF
    160 170 180 190 200
    KAMEPDAPLL REDIKDKMTG AHGARKHHNH IFRWEIGDKE GTDATFAKAE
    210 220 230 240 250
    VVSKDMFTYH RVHPSPLETC QCVASMDKIK GELTLWGTFQ APHVIRTVVS
    260 270 280 290 300
    LISGLPEHKI HVIAPDIGGG FGNKVGAYSG YVCAVVASIV LGVPVKWVED
    310 320 330 340 350
    RMENLSTTSF ARDYHMTTEL AATKDGKILA MRCHVLADHG AFDACADPSK
    360 370 380 390 400
    WPAGFMNICT GSYDMPVAHL AVDGVYTNKA SGGVAYRCSF RVTEAVYAIE
    410 420 430 440 450
    RAIETLAQRL EMDSADLRIK NFIQPEQFPY MAPLGWEYDS GNYPLAMKKA
    460 470 480 490 500
    MDTVGYHQLR AEQKAKQEAF KRGETREIMG IGISFFTEIV GAGPSKNCDI
    510 520 530 540 550
    LGVSMFDSAE IRIHPTGSVI ARMGTKSQGQ GHETTYAQII ATELGIPADD
    560 570 580 590 600
    IMIEEGNTDT APYGLGTYGS RSTPTAGAAT AVAARKIKAK AQMIAAHMLE
    610 620 630 640 650
    VHEGDLEWDV DRFRVKGLPE KFKTMKELAW ASYNSPPPNL EPGLEAVNYY
    660 670 680 690 700
    DPPNMTYPFG AYFCIMDIDV DTGVAKTRRF YALDDCGTRI NPMIIEGQVH
    710 720 730 740 750
    GGLTEAFAVA MGQEIRYDEQ GNVLGASFMD FFLPTAVETP KWETDYTVTP
    760 770 780 790 800
    SPHHPIGAKG VGESPHVGGV PCFSNAVNDA YAFLNAGHIQ MPHDAWRLWK

    VGEQLGLHV
    Length:809
    Mass (Da):88,739
    Last modified:July 11, 2002 - v2
    Checksum:i7F2F3D0EC996BDBD
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti6Missing AA sequence (PubMed:2818128).Curated1
    Sequence conflicti11 – 12SA → AG AA sequence (PubMed:2818128).Curated2

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP002827 Genomic DNA. Translation: AEI08106.1.
    PIRiC56279.
    RefSeqiWP_013913730.1. NC_015689.1.

    Genome annotation databases

    EnsemblBacteriaiAEI08106; AEI08106; OCA5_pHCG300310.
    KEGGiocg:OCA5_pHCG300310.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP002827 Genomic DNA. Translation: AEI08106.1.
    PIRiC56279.
    RefSeqiWP_013913730.1. NC_015689.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1N5WX-ray1.50B/E1-809[»]
    1N60X-ray1.19B/E1-809[»]
    1N61X-ray1.30B/E1-809[»]
    1N62X-ray1.09B/E1-809[»]
    1N63X-ray1.21B/E1-809[»]
    1ZXIX-ray1.70B/E1-809[»]
    ProteinModelPortaliP19919.
    SMRiP19919.
    ModBaseiSearch...
    MobiDBiSearch...

    Proteomic databases

    PRIDEiP19919.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAEI08106; AEI08106; OCA5_pHCG300310.
    KEGGiocg:OCA5_pHCG300310.

    Phylogenomic databases

    KOiK03520.
    OMAiPYPEDYI.

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-19674.
    BRENDAi1.2.99.2. 4399.

    Miscellaneous databases

    EvolutionaryTraceiP19919.

    Family and domain databases

    Gene3Di3.30.365.10. 6 hits.
    3.90.1170.50. 1 hit.
    InterProiIPR000674. Ald_Oxase/Xan_DH_a/b.
    IPR008274. AldOxase/xan_DH_Mopterin-bd.
    IPR012780. CO_Mo_DH_lsu.
    [Graphical view]
    PfamiPF01315. Ald_Xan_dh_C. 1 hit.
    PF02738. Ald_Xan_dh_C2. 1 hit.
    [Graphical view]
    SMARTiSM01008. Ald_Xan_dh_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF54665. SSF54665. 1 hit.
    SSF56003. SSF56003. 1 hit.
    TIGRFAMsiTIGR02416. CO_dehy_Mo_lg. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiDCML_OLICO
    AccessioniPrimary (citable) accession number: P19919
    Secondary accession number(s): F8C0Z6, Q51325
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: July 11, 2002
    Last modified: November 2, 2016
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.