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Protein

Carbon monoxide dehydrogenase medium chain

Gene

cutM

Organism
Hydrogenophaga pseudoflava (Pseudomonas carboxydoflava)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of carbon monoxide to carbon dioxide.

Catalytic activityi

CO + H2O + A = CO2 + AH2.

Cofactori

FADNote: Binds 1 FAD per subunit.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi32 – 365FAD
Nucleotide bindingi111 – 1155FAD

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Carbon monoxide dehydrogenase medium chain (EC:1.2.99.2)
Short name:
CO dehydrogenase subunit M
Short name:
CO-DH M
Gene namesi
Name:cutM
OrganismiHydrogenophaga pseudoflava (Pseudomonas carboxydoflava)
Taxonomic identifieri47421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeHydrogenophaga

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 287287Carbon monoxide dehydrogenase medium chainPRO_0000079812Add
BLAST

Interactioni

Subunit structurei

Dimer of heterotrimers. Each heterotrimer consists of a large, a medium and a small subunit.

Structurei

Secondary structure

1
287
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 93Combined sources
Helixi14 – 2411Combined sources
Helixi25 – 273Combined sources
Beta strandi28 – 336Combined sources
Helixi37 – 426Combined sources
Beta strandi49 – 535Combined sources
Helixi58 – 603Combined sources
Beta strandi63 – 664Combined sources
Beta strandi69 – 735Combined sources
Helixi78 – 836Combined sources
Helixi85 – 906Combined sources
Helixi92 – 976Combined sources
Helixi98 – 1003Combined sources
Helixi106 – 1083Combined sources
Helixi112 – 1176Combined sources
Helixi125 – 1317Combined sources
Beta strandi135 – 1406Combined sources
Beta strandi143 – 1497Combined sources
Beta strandi153 – 1553Combined sources
Beta strandi158 – 1603Combined sources
Beta strandi166 – 1738Combined sources
Beta strandi180 – 1867Combined sources
Beta strandi195 – 20511Combined sources
Beta strandi208 – 22215Combined sources
Helixi227 – 2315Combined sources
Helixi240 – 25213Combined sources
Helixi264 – 28522Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FFUX-ray2.35C/F1-287[»]
1FFVX-ray2.25C/F1-287[»]
ProteinModelPortaliP19914.
SMRiP19914. Positions 1-287.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP19914.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 177177FAD-binding PCMH-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FAD-binding PCMH-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
InterProiIPR005107. CO_DH_flav_C.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR002346. Mopterin_DH_FAD-bd.
[Graphical view]
PfamiPF03450. CO_deh_flav_C. 1 hit.
PF00941. FAD_binding_5. 1 hit.
[Graphical view]
SMARTiSM01092. CO_deh_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF55447. SSF55447. 1 hit.
SSF56176. SSF56176. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19914-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPPRFEYHA PKSVGEAVAL LGQLGSDAKL LAGGHSLLPM MKLRFAQPEH
60 70 80 90 100
LIDINRIPEL RGIREEGSTV VIGAMTVEND LISSPIVQAR LPLLAEAAKL
110 120 130 140 150
IADPQVRNRG TIGGDIAHGH PGNDHPALSI AVEAHFVLEG PNGRRTVPAD
160 170 180 190 200
GFFLGTYMTL LEENEVMVEI RVPAFAQGTG WAYEKLKRKT GDWATAGCAV
210 220 230 240 250
VMRKSGNTVS HIRIALTNVA PTALRREGGR SRLLGKAFTK EAVQAAADAA
260 270 280
IAICEPAEDL RGDADYKTAM AGQMVKRALN AAWARCA
Length:287
Mass (Da):30,722
Last modified:July 11, 2002 - v2
Checksum:iF3E9848BDCF4138E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 32IP → MI AA sequence (PubMed:2818128).Curated
Sequence conflicti13 – 131S → H AA sequence (PubMed:2818128).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U80806 Genomic DNA. Translation: AAD00361.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U80806 Genomic DNA. Translation: AAD00361.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FFUX-ray2.35C/F1-287[»]
1FFVX-ray2.25C/F1-287[»]
ProteinModelPortaliP19914.
SMRiP19914. Positions 1-287.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP19914.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
InterProiIPR005107. CO_DH_flav_C.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR002346. Mopterin_DH_FAD-bd.
[Graphical view]
PfamiPF03450. CO_deh_flav_C. 1 hit.
PF00941. FAD_binding_5. 1 hit.
[Graphical view]
SMARTiSM01092. CO_deh_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF55447. SSF55447. 1 hit.
SSF56176. SSF56176. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and molecular characterization of the genes for carbon monoxide dehydrogenase and localization of molybdopterin, flavin adenine dinucleotide, and iron-sulfur centers in the enzyme of Hydrogenophaga pseudoflava."
    Kang B.S., Kim Y.M.
    J. Bacteriol. 181:5581-5590(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Homology and distribution of CO dehydrogenase structural genes in carboxydotrophic bacteria."
    Kraut M., Hugendieck I., Herwig S., Meyer O.
    Arch. Microbiol. 152:335-341(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-14.
  3. "The effect of intracellular molybdenum in Hydrogenophaga pseudoflava on the crystallographic structure of the seleno-molybdo-iron-sulfur flavoenzyme carbon monoxide dehydrogenase."
    Haenzelmann P., Dobbek H., Gremer L., Huber R., Meyer O.
    J. Mol. Biol. 301:1221-1235(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS).
  4. "The role of Se, Mo and Fe in the structure and function of carbon monoxide dehydrogenase."
    Meyer O., Gremer L., Ferner R., Ferner M., Dobbek H., Gnida M., Meyer-Klaucke W., Huber R.
    Biol. Chem. 381:865-876(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiDCMM_HYDPS
AccessioniPrimary (citable) accession number: P19914
Secondary accession number(s): Q9ZAR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: July 11, 2002
Last modified: November 11, 2015
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.