P19911 (F16A2_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fructose-1,6-bisphosphatase class 1 2
Short name=FBPase class 1 2
EC=3.1.3.11

Alternative name(s):
D-fructose-1,6-bisphosphate 1-phosphohydrolase class 1 2

Gene names

Name:	fbp2
Synonyms:	cbbF2, cbbFC, cfxF
Ordered Locus Names:	H16_B1390

Organism

Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate. HAMAP MF_01855
Cofactor	Binds 2 magnesium ions per subunit By similarity. HAMAP MF_01855
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_01855
Subunit structure	Homotetramer By similarity. HAMAP MF_01855
Subcellular location	Cytoplasm Potential HAMAP MF_01855.
Sequence similarities	Belongs to the FBPase class 1 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fructose 1,6-bisphosphate 1-phosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Fructose-1,6-bisphosphatase class 1 2 HAMAP MF_01855		PRO_0000200479
Regions
	Region	126 – 129	4	Substrate binding By similarity
Sites
	Metal binding	101	1	Magnesium 1 By similarity
	Metal binding	123	1	Magnesium 1 By similarity
	Metal binding	123	1	Magnesium 2 By similarity
	Metal binding	125	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	126	1	Magnesium 2 By similarity
	Metal binding	290	1	Magnesium 2 By similarity
	Binding site	218	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P19911 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 0BD30D7CBBCDCC23	FASTA	364	39,607
	10 20 30 40 50 60 MPEVQRMTLT QFLIEERRRY PDASGGFNGL ILNVAMACKE IARAVAFGAL GGLHGKASNQ 70 80 90 100 110 120 AGEAGAVNVQ GEIQQKLDVL SNTTFLRVNE WGGYLAGMAS EEMEAPYQIP DHYPRGKYLL 130 140 150 160 170 180 VFDPLDGSSN IDVNVSVGSI FSVLRAPEGA SAVTEQDFLQ PGSAQVAAGY ALYGPTTMLV 190 200 210 220 230 240 LTVGNGVNGF TLDPNLGEFF LTHPNLQVPA DTQEFAINAS NSRFWEAPVQ RYIAECMAGK 250 260 270 280 290 300 SGPRGKDFNM RWIASMVAEA HRILMRGGVF MYPRDSKDPA KPGRLRLLYE ANPIAFLMEQ 310 320 330 340 350 360 AGGRASTGRQ TLMSVAPGAL HQRIGVIFGS RNEVERIEGY HTDQTDPDLP SPLFNERSLF RASA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the cbbF genes from Alcaligenes eutrophus that encode fructose-1,6-/sedoheptulose-1,7-bisphosphatase." Yoo J.-G., Bowien B. Curr. Microbiol. 31:55-61(1995) [PubMed: 7767230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16." Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B. Nat. Biotechnol. 24:1257-1262(2006) [PubMed: 16964242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.
[3]	"Sequence analysis of the chromosomal and plasmid genes encoding phosphoribulokinase from Alcaligenes eutrophus." Kossmann J., Klintworth R., Bowien B. Gene 85:247-252(1989) [PubMed: 2559876] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 206-364.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U16791 Genomic DNA. Translation: AAA69975.1. AM260480 Genomic DNA. Translation: CAJ96179.1. M33563 Genomic DNA. Translation: AAA21958.1.
PIR	I39556.
RefSeq	YP_840909.1. NC_008314.1.
3D structure databases
ProteinModelPortal	P19911.
ModBase	Search...
Protein-protein interaction databases
STRING	P19911.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4456349.
GenomeReviews	Gene locus H16_B1390 in contig AM260480_GR.
KEGG	reh:H16_B1390.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0158.
HOGENOM	HBG731261.
OMA	RYVEECI.
PhylomeDB	P19911.
ProtClustDB	CLSK923881.
Enzyme and pathway databases
BioCyc	REUT381666:H16_B1390-MONOMER.
Family and domain databases
HAMAP	MF_01855. FBPase_class1. [Tree]
InterPro	IPR000146. FBPase_class-1/SBPase. IPR020548. Fructose_bisphosphatase_AS. [Graphical view]
KO	K03841.
PANTHER	PTHR11556. In_FB_phphtase. 1 hit.
Pfam	PF00316. FBPase. 1 hit. [Graphical view]
PIRSF	PIRSF000904. FBPtase_SBPase. 1 hit.
PRINTS	PR00115. F16BPHPHTASE.
PROSITE	PS00124. FBPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F16A2_CUPNH

Accession

Primary (citable) accession number: P19911
Secondary accession number(s): Q0K1E5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	October 1, 1996
Last modified:	November 16, 2011
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program