Reviewed, UniProtKB/Swiss-Prot P19908 (LUXB_VIBFI)

Names and origin

Protein names

Recommended name:
    Alkanal monooxygenase beta chain
    EC=1.14.14.3
Alternative name(s):
    Bacterial luciferase beta chain

Gene names

Name:

luxB

Organism

Vibrio fischeri

Taxonomic identifier

668 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Light-emitting reaction in luminous bacteria. The specific role of the beta subunit is unknown, but it is absolutely required for bioluminescence activity.
Catalytic activity	RCHO + reduced FMN + O2 = RCOOH + FMN + H2O + light.
Subunit structure	Heterodimer of an alpha and a beta chain.
Sequence similarities	Belongs to the bacterial luciferase oxidoreductase family.

Ontologies

Keywords
Biological process	Luminescence
Ligand	FMN Flavoprotein
Molecular function	Monooxygenase Oxidoreductase Photoprotein
Gene Ontology (GO)
Biological process	bioluminescence Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alkanal monooxygenase (FMN-linked) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Alkanal monooxygenase beta chain		PRO_0000220180

Sequences

	Sequence		Length	Mass (Da)
	P19908-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 228C0FB433F79F75	FASTA	326	37,357
	10 20 30 40 50 60 MKFGLFFLNF QKDGITSEET LDNMVKTVTL IDSTKYHFNT AFVNEHHFSK NGIVGAPITA 70 80 90 100 110 120 AGFLLGLTNK LHIGSLNQVI TTHHPVRVAE EASLLDQMSE GRFILGFSDC ESDFEMEFFR 130 140 150 160 170 180 RHISSRQQQF EACYEIINDA LTTGYCHPQN DFYDFPKVSI NPHCYSENGP KQYVSATSKE 190 200 210 220 230 240 VVMWAAKKAL PLTFKWEDNL ETKERYAILY NKTAQQYGID ISDVDHQLTV IANLNADRST 250 260 270 280 290 300 AQEEVREYLK DYITETYPQM DRDEKINCII EENAVGSHDD YYESTKLAVE KTGSKNILLS 310 320 FESMSDIKDV KDIIDMLNQK IEMNLP

References

[1]	"Nucleotide sequence of the LuxA and LuxB genes of the bioluminescent marine bacterium Vibrio fischeri." Foran D.R., Brown W.M. Nucleic Acids Res. 16:777-777(1988) [PubMed: 3340562] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MJ-1.
[2]	"Vibrio fischeri Lux operon SalI digest." Knight T., Papadakis N. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MJ-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X06758 Genomic DNA. Translation: CAA29932.1. Sequence problems. U55385 Genomic DNA. Translation: AAB00443.1. U55819 Genomic DNA. Translation: AAB00450.1. AF170104 Genomic DNA. Translation: AAD48478.1.
PIR	S00575.
3D structure databases
HSSP	HSSP built from PDB template 1LUC based on UniProtKB P07739.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.14.3. 350.
Family and domain databases
InterPro	IPR018235. Bacterial_luciferase_CS. IPR011251. Luciferase-like. IPR016048. Luciferase-like_sub. IPR002103. Luciferase_bac. [Graphical view]
Gene3D	G3DSA:3.20.20.30. Luciferase_like. 1 hit.
Pfam	PF00296. Bac_luciferase. 1 hit. [Graphical view]
PRINTS	PR00089. LUCIFERASE.
PROSITE	PS00494. BACTERIAL_LUCIFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LUXB_VIBFI

Accession

Primary (citable) accession number: P19908

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)