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Protein

Glutamine synthetase

Gene

glnA

Organism
Vibrio alginolyticus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Enzyme regulationi

The activity of this enzyme could be controlled by adenylation under conditions of abundant glutamine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi130Magnesium 1By similarity1
Metal bindingi132Magnesium 2By similarity1
Binding sitei208ATPBy similarity1
Metal bindingi213Magnesium 2By similarity1
Metal bindingi220Magnesium 2By similarity1
Binding sitei265L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi269Magnesium 1; via pros nitrogenBy similarity1
Binding sitei273ATPBy similarity1
Binding sitei321L-glutamateBy similarity1
Binding sitei327L-glutamateBy similarity1
Binding sitei339ATPBy similarity1
Binding sitei339L-glutamateBy similarity1
Binding sitei344ATPBy similarity1
Binding sitei352ATPBy similarity1
Metal bindingi357Magnesium 1By similarity1
Binding sitei359L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi271 – 273ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseCurated
Glutamine synthetase I betaBy similarity
Short name:
GSI betaBy similarity
Gene namesi
Name:glnABy similarity
OrganismiVibrio alginolyticus
Taxonomic identifieri663 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532751 – 468Glutamine synthetaseAdd BLAST468

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei397O-AMP-tyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP19904.

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexameric ring.By similarity

Protein-protein interaction databases

STRINGi674977.VMC_02600.

Structurei

3D structure databases

ProteinModelPortaliP19904.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni264 – 265L-glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4105C5F. Bacteria.
COG0174. LUCA.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

P19904-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVEKVLSLI QENEVKFVDL RFTDTKGKEQ HISIPAHQID ADFFEEGKMF
60 70 80 90 100
DGSSVAGWKG INESDMVMMP DASSAVLDPF TEDATLNIRC DILEPATMQG
110 120 130 140 150
YDRDPRSIAK RAEDFMRSTG VADTVLIGPE PEFFLFDDVK FATDMSGSFF
160 170 180 190 200
KIDDVEAAWN TGSDYEEGNK GHRPGVKGGY FPVAPVDSSQ DIRSAMCLVM
210 220 230 240 250
EEMGLVVEAH HHEATAGQNE IATRFNTLTT KADEIQIYKY VVHNVAHAFG
260 270 280 290 300
KTATFMPKPL VGDNGSGMHV HQSLAKDGVN LFAGDKYGGL SEMALYYIGG
310 320 330 340 350
IIKHARAINA FANPSTNSYK RLVPGFEAPV MLAYSARNRS ASIRIPVVPS
360 370 380 390 400
PKARRIEVRF GDPAANPYLC FASMLMAGLD GIKNKIHPGE AMDKDLYDLP
410 420 430 440 450
AEESAEIPTV AYSLKDALAE LDADREFLTA GGVFSDDFID SYIELKSQDV
460
ERVNMTTHPV EFELYYSV
Length:468
Mass (Da):51,556
Last modified:February 1, 1991 - v1
Checksum:iABB66AA9C072D856
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08499 Genomic DNA. Translation: AAA27523.1.
PIRiJL0113. AJVCQA.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08499 Genomic DNA. Translation: AAA27523.1.
PIRiJL0113. AJVCQA.

3D structure databases

ProteinModelPortaliP19904.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi674977.VMC_02600.

Proteomic databases

PRIDEiP19904.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105C5F. Bacteria.
COG0174. LUCA.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLN1B_VIBAL
AccessioniPrimary (citable) accession number: P19904
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: March 15, 2017
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.