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P19899

- CAPSD_MNSV

UniProt

P19899 - CAPSD_MNSV

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Protein

Capsid protein

Gene

ORF4

Organism
Melon necrotic spot virus (MNSV)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Capsid protein self-assembles to form an icosahedral capsid with a T=3 symmetry, about 32-35 nm in diameter, and consisting of 180 capsid proteins. Also acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs (By similarity).By similarity

Cofactori

Binds 4 calcium ions per icosahedral asymmetric unit, itself composed of three capsid protein subunits. Calcium ions probably promote virus assembly and stabilize the virus particle.

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. structural molecule activity Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Calcium, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Capsid protein
Alternative name(s):
Coat protein
p42
Gene namesi
ORF Names:ORF4
OrganismiMelon necrotic spot virus (MNSV)
Taxonomic identifieri11987 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageTombusviridaeCarmovirus
Virus hostiCucumis melo (Muskmelon) [TaxID: 3656]
Cucumis sativus (Cucumber) [TaxID: 3659]

Subcellular locationi

Virion By similarity

GO - Cellular componenti

  1. T=3 icosahedral viral capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, T=3 icosahedral capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 390390Capsid proteinPRO_0000222864Add
BLAST

Interactioni

Subunit structurei

Homodimer. Homomultimer.By similarity

Structurei

Secondary structure

1
390
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi96 – 10712
Helixi116 – 1183
Turni122 – 1243
Beta strandi125 – 1273
Turni132 – 1343
Turni136 – 1383
Helixi139 – 1424
Beta strandi145 – 15915
Beta strandi168 – 1758
Helixi185 – 1884
Beta strandi192 – 1976
Beta strandi203 – 2075
Helixi224 – 2263
Beta strandi231 – 2388
Beta strandi241 – 2433
Beta strandi246 – 25611
Beta strandi265 – 2717
Turni273 – 2753
Beta strandi281 – 2855
Helixi290 – 2923
Beta strandi293 – 2964
Beta strandi299 – 3024
Beta strandi308 – 32013
Beta strandi328 – 34114
Beta strandi344 – 35310
Beta strandi359 – 3613
Beta strandi363 – 3664
Beta strandi374 – 3829
Helixi384 – 3863

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZAHX-ray2.81A/B/C60-390[»]
ProteinModelPortaliP19899.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP19899.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9494R domain, disordered, interaction with RNAAdd
BLAST
Regioni95 – 256162S domain, virion shellAdd
BLAST
Regioni257 – 390134P domain, projectingAdd
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.120.20. 1 hit.
InterProiIPR000937. Capsid_prot_S-dom_vir.
IPR029053. Viral_coat.
[Graphical view]
PfamiPF00729. Viral_coat. 1 hit.
[Graphical view]
PRINTSiPR00233. ICOSAHEDRAL.
PROSITEiPS00555. ICOSAH_VIR_COAT_S. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19899-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAMVKRINNL PTVKLAKQAL PLLANPKLVN KAIDVVPLVV QGGRKLSKAA
60 70 80 90 100
KRLLGAYGGN ISYTEGAKPG AISAPVAISR RVAGMKPRFV RSEGSVKIVH
110 120 130 140 150
REFIASVLPS SDLTVNNGDV NIGKYRVNPS NNALFTWLQG QAQLYDMYRF
160 170 180 190 200
TRLRITYIPT TGSTSTGRVS LLWDRDSQDP LPIDRAAISS YAHSADSAPW
210 220 230 240 250
AENVLVVPCD NTWRYMNDTN AVDRKLVDFG QFLFATYSGA GSTAHGDLYV
260 270 280 290 300
EYAVEFKDPQ PIAGMVCMFD RLVSLSEVGS TIKGVNYIAD RDVITTGGNI
310 320 330 340 350
GVNINIPGTY LVTIVLNATS IGPLTFTGNS KLVGNSLNLT SSGASALTFT
360 370 380 390
LNSTGVPNSS DSSFSVGTVV ALTRVRMTIT RCSPETAYLA
Length:390
Mass (Da):41,853
Last modified:February 1, 1991 - v1
Checksum:i62D6AF207BC7EA03
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M29671 Genomic RNA. Translation: AAB02435.1.
D00562 Genomic RNA. Translation: BAA00436.1.
D12536 Genomic RNA. Translation: BAA02104.1.
PIRiJQ0169. VCVEMN.
RefSeqiNP_041231.1. NC_001504.1.

Genome annotation databases

GeneIDi1491981.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M29671 Genomic RNA. Translation: AAB02435.1 .
D00562 Genomic RNA. Translation: BAA00436.1 .
D12536 Genomic RNA. Translation: BAA02104.1 .
PIRi JQ0169. VCVEMN.
RefSeqi NP_041231.1. NC_001504.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2ZAH X-ray 2.81 A/B/C 60-390 [» ]
ProteinModelPortali P19899.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1491981.

Miscellaneous databases

EvolutionaryTracei P19899.

Family and domain databases

Gene3Di 2.60.120.20. 1 hit.
InterProi IPR000937. Capsid_prot_S-dom_vir.
IPR029053. Viral_coat.
[Graphical view ]
Pfami PF00729. Viral_coat. 1 hit.
[Graphical view ]
PRINTSi PR00233. ICOSAHEDRAL.
PROSITEi PS00555. ICOSAH_VIR_COAT_S. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Coat protein of melon necrotic spot carmovirus is more similar to those of tombusviruses than those of carmoviruses."
    Riviere C.J., Pot J., Tremaine J.H., Rochon D.M.
    J. Gen. Virol. 70:3033-3042(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Nucleotide sequence and genomic organization of melon necrotic spot virus."
    Riviere C.J., Rochon D.M.
    J. Gen. Virol. 71:1887-1896(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "The structure of melon necrotic spot virus determined at 2.8 A resolution."
    Wada Y., Tanaka H., Yamashita E., Kubo C., Ichiki-Uehara T., Nakazono-Nagaoka E., Omura T., Tsukihara T.
    Acta Crystallogr. F 64:8-13(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.81 ANGSTROMS) OF 60-390.
    Strain: KS.

Entry informationi

Entry nameiCAPSD_MNSV
AccessioniPrimary (citable) accession number: P19899
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: October 1, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3