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P19899 (CAPSD_MNSV) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Capsid protein
Alternative name(s):
Coat protein
p42
Gene names
ORF Names:ORF4
OrganismMelon necrotic spot virus (MNSV)
Taxonomic identifier11987 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stageTombusviridaeCarmovirus
Virus hostCucumis melo (Muskmelon) [TaxID: 3656]
Cucumis sativus (Cucumber) [TaxID: 3659]

Protein attributes

Sequence length390 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Capsid protein self-assembles to form an icosahedral capsid with a T=3 symmetry, about 32-35 nm in diameter, and consisting of 180 capsid proteins. Also acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs By similarity.

Cofactor

Binds 4 calcium ions per icosahedral asymmetric unit, itself composed of three capsid protein subunits. Calcium ions probably promote virus assembly and stabilize the virus particle.

Subunit structure

Homodimer By similarity. Homomultimer By similarity.

Subcellular location

Virion By similarity.

Sequence similarities

Belongs to the icosahedral plant coat protein family.

Ontologies

Keywords
   Cellular componentCapsid protein
T=3 icosahedral capsid protein
Virion
   LigandCalcium
RNA-binding
   Technical term3D-structure
Gene Ontology (GO)
   Cellular_componentT=3 icosahedral viral capsid

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

structural molecule activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 390390Capsid protein
PRO_0000222864

Regions

Region1 – 9494R domain, disordered, interaction with RNA
Region95 – 256162S domain, virion shell
Region257 – 390134P domain, projecting

Secondary structure

........................................................ 390
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P19899 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 62D6AF207BC7EA03

FASTA39041,853
        10         20         30         40         50         60 
MAMVKRINNL PTVKLAKQAL PLLANPKLVN KAIDVVPLVV QGGRKLSKAA KRLLGAYGGN 

        70         80         90        100        110        120 
ISYTEGAKPG AISAPVAISR RVAGMKPRFV RSEGSVKIVH REFIASVLPS SDLTVNNGDV 

       130        140        150        160        170        180 
NIGKYRVNPS NNALFTWLQG QAQLYDMYRF TRLRITYIPT TGSTSTGRVS LLWDRDSQDP 

       190        200        210        220        230        240 
LPIDRAAISS YAHSADSAPW AENVLVVPCD NTWRYMNDTN AVDRKLVDFG QFLFATYSGA 

       250        260        270        280        290        300 
GSTAHGDLYV EYAVEFKDPQ PIAGMVCMFD RLVSLSEVGS TIKGVNYIAD RDVITTGGNI 

       310        320        330        340        350        360 
GVNINIPGTY LVTIVLNATS IGPLTFTGNS KLVGNSLNLT SSGASALTFT LNSTGVPNSS 

       370        380        390 
DSSFSVGTVV ALTRVRMTIT RCSPETAYLA 

« Hide

References

[1]"Coat protein of melon necrotic spot carmovirus is more similar to those of tombusviruses than those of carmoviruses."
Riviere C.J., Pot J., Tremaine J.H., Rochon D.M.
J. Gen. Virol. 70:3033-3042(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Nucleotide sequence and genomic organization of melon necrotic spot virus."
Riviere C.J., Rochon D.M.
J. Gen. Virol. 71:1887-1896(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]"The structure of melon necrotic spot virus determined at 2.8 A resolution."
Wada Y., Tanaka H., Yamashita E., Kubo C., Ichiki-Uehara T., Nakazono-Nagaoka E., Omura T., Tsukihara T.
Acta Crystallogr. F 64:8-13(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.81 ANGSTROMS) OF 60-390.
Strain: KS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M29671 Genomic RNA. Translation: AAB02435.1.
D00562 Genomic RNA. Translation: BAA00436.1.
D12536 Genomic RNA. Translation: BAA02104.1.
PIRVCVEMN. JQ0169.
RefSeqNP_041231.1. NC_001504.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZAHX-ray2.81A/B/C60-390[»]
ProteinModelPortalP19899.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1491981.

Family and domain databases

Gene3D2.60.120.20. 1 hit.
InterProIPR000937. Capsid_prot_S-dom_vir.
IPR029053. Viral_coat.
[Graphical view]
PfamPF00729. Viral_coat. 1 hit.
[Graphical view]
PRINTSPR00233. ICOSAHEDRAL.
PROSITEPS00555. ICOSAH_VIR_COAT_S. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP19899.

Entry information

Entry nameCAPSD_MNSV
AccessionPrimary (citable) accession number: P19899
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: July 9, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references