Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P19899

- CAPSD_MNSV

UniProt

P19899 - CAPSD_MNSV

Protein

Capsid protein

Gene

ORF4

Organism
Melon necrotic spot virus (MNSV)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Capsid protein self-assembles to form an icosahedral capsid with a T=3 symmetry, about 32-35 nm in diameter, and consisting of 180 capsid proteins. Also acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs By similarity.By similarity

    Cofactori

    Binds 4 calcium ions per icosahedral asymmetric unit, itself composed of three capsid protein subunits. Calcium ions probably promote virus assembly and stabilize the virus particle.

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW
    2. structural molecule activity Source: InterPro

    Keywords - Ligandi

    Calcium, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Capsid protein
    Alternative name(s):
    Coat protein
    p42
    Gene namesi
    ORF Names:ORF4
    OrganismiMelon necrotic spot virus (MNSV)
    Taxonomic identifieri11987 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageTombusviridaeCarmovirus
    Virus hostiCucumis melo (Muskmelon) [TaxID: 3656]
    Cucumis sativus (Cucumber) [TaxID: 3659]

    Subcellular locationi

    Virion By similarity

    GO - Cellular componenti

    1. T=3 icosahedral viral capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, T=3 icosahedral capsid protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 390390Capsid proteinPRO_0000222864Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer. Homomultimer.By similarity

    Structurei

    Secondary structure

    1
    390
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi96 – 10712
    Helixi116 – 1183
    Turni122 – 1243
    Beta strandi125 – 1273
    Turni132 – 1343
    Turni136 – 1383
    Helixi139 – 1424
    Beta strandi145 – 15915
    Beta strandi168 – 1758
    Helixi185 – 1884
    Beta strandi192 – 1976
    Beta strandi203 – 2075
    Helixi224 – 2263
    Beta strandi231 – 2388
    Beta strandi241 – 2433
    Beta strandi246 – 25611
    Beta strandi265 – 2717
    Turni273 – 2753
    Beta strandi281 – 2855
    Helixi290 – 2923
    Beta strandi293 – 2964
    Beta strandi299 – 3024
    Beta strandi308 – 32013
    Beta strandi328 – 34114
    Beta strandi344 – 35310
    Beta strandi359 – 3613
    Beta strandi363 – 3664
    Beta strandi374 – 3829
    Helixi384 – 3863

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2ZAHX-ray2.81A/B/C60-390[»]
    ProteinModelPortaliP19899.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP19899.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 9494R domain, disordered, interaction with RNAAdd
    BLAST
    Regioni95 – 256162S domain, virion shellAdd
    BLAST
    Regioni257 – 390134P domain, projectingAdd
    BLAST

    Sequence similaritiesi

    Family and domain databases

    Gene3Di2.60.120.20. 1 hit.
    InterProiIPR000937. Capsid_prot_S-dom_vir.
    IPR029053. Viral_coat.
    [Graphical view]
    PfamiPF00729. Viral_coat. 1 hit.
    [Graphical view]
    PRINTSiPR00233. ICOSAHEDRAL.
    PROSITEiPS00555. ICOSAH_VIR_COAT_S. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P19899-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAMVKRINNL PTVKLAKQAL PLLANPKLVN KAIDVVPLVV QGGRKLSKAA    50
    KRLLGAYGGN ISYTEGAKPG AISAPVAISR RVAGMKPRFV RSEGSVKIVH 100
    REFIASVLPS SDLTVNNGDV NIGKYRVNPS NNALFTWLQG QAQLYDMYRF 150
    TRLRITYIPT TGSTSTGRVS LLWDRDSQDP LPIDRAAISS YAHSADSAPW 200
    AENVLVVPCD NTWRYMNDTN AVDRKLVDFG QFLFATYSGA GSTAHGDLYV 250
    EYAVEFKDPQ PIAGMVCMFD RLVSLSEVGS TIKGVNYIAD RDVITTGGNI 300
    GVNINIPGTY LVTIVLNATS IGPLTFTGNS KLVGNSLNLT SSGASALTFT 350
    LNSTGVPNSS DSSFSVGTVV ALTRVRMTIT RCSPETAYLA 390
    Length:390
    Mass (Da):41,853
    Last modified:February 1, 1991 - v1
    Checksum:i62D6AF207BC7EA03
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M29671 Genomic RNA. Translation: AAB02435.1.
    D00562 Genomic RNA. Translation: BAA00436.1.
    D12536 Genomic RNA. Translation: BAA02104.1.
    PIRiJQ0169. VCVEMN.
    RefSeqiNP_041231.1. NC_001504.1.

    Genome annotation databases

    GeneIDi1491981.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M29671 Genomic RNA. Translation: AAB02435.1 .
    D00562 Genomic RNA. Translation: BAA00436.1 .
    D12536 Genomic RNA. Translation: BAA02104.1 .
    PIRi JQ0169. VCVEMN.
    RefSeqi NP_041231.1. NC_001504.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2ZAH X-ray 2.81 A/B/C 60-390 [» ]
    ProteinModelPortali P19899.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1491981.

    Miscellaneous databases

    EvolutionaryTracei P19899.

    Family and domain databases

    Gene3Di 2.60.120.20. 1 hit.
    InterProi IPR000937. Capsid_prot_S-dom_vir.
    IPR029053. Viral_coat.
    [Graphical view ]
    Pfami PF00729. Viral_coat. 1 hit.
    [Graphical view ]
    PRINTSi PR00233. ICOSAHEDRAL.
    PROSITEi PS00555. ICOSAH_VIR_COAT_S. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Coat protein of melon necrotic spot carmovirus is more similar to those of tombusviruses than those of carmoviruses."
      Riviere C.J., Pot J., Tremaine J.H., Rochon D.M.
      J. Gen. Virol. 70:3033-3042(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Nucleotide sequence and genomic organization of melon necrotic spot virus."
      Riviere C.J., Rochon D.M.
      J. Gen. Virol. 71:1887-1896(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    3. "The structure of melon necrotic spot virus determined at 2.8 A resolution."
      Wada Y., Tanaka H., Yamashita E., Kubo C., Ichiki-Uehara T., Nakazono-Nagaoka E., Omura T., Tsukihara T.
      Acta Crystallogr. F 64:8-13(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.81 ANGSTROMS) OF 60-390.
      Strain: KS.

    Entry informationi

    Entry nameiCAPSD_MNSV
    AccessioniPrimary (citable) accession number: P19899
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3