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P19888 (MTBA_ANEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase BanI

Short name=M.BanI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase BanI
Gene names
Name:banIM
OrganismAneurinibacillus aneurinilyticus (Bacillus aneurinolyticus)
Taxonomic identifier1391 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeAneurinibacillus groupAneurinibacillus

Protein attributes

Sequence length428 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GGYRCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the BanI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 428428Modification methylase BanI
PRO_0000087860

Regions

Domain3 – 417415SAM-dependent MTase C5-type

Sites

Active site761 By similarity

Sequences

Sequence LengthMass (Da)Tools
P19888 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: ADF3E932A681E43E

FASTA42848,618
        10         20         30         40         50         60 
MKIKFVDLFA GIGGIRIGFE RAAKRFELET ECVLSSEIDK KACETYALNF KEEPQGDIHE 

        70         80         90        100        110        120 
ITSFPEFDFL LAGFPCQPFS YAGKQQGFGD TRGTLFFEVE RVLRDNRPKA FLLENVRGLV 

       130        140        150        160        170        180 
THDKGRTLKT IISKLEELGY GVSYLLLNSS TFGVPQNRVR IYILGILGSK PKLTLTSNVG 

       190        200        210        220        230        240 
AADSHKYKNE QISLFDESYA TVKDILEDSP SEKYRCSDEF IGQLSKVVGN NFELLHGYRL 

       250        260        270        280        290        300 
IDYRGGNSIH SWELGIKGDC TKEEIEFLNQ LIANRRKKIY GTHQDGKALT LEQIRTFYNH 

       310        320        330        340        350        360 
DQLEVIIKSL LQKGYLREEE NKFNPVCGNM SFEVFKFLDP DSISITLTSS DAHKLGVVQN 

       370        380        390        400        410        420 
NVPRRITPRE CARLQGFPDD FILHSNDNFA YKQLGNSVTV KVVEKVIEDL FQNNVNELFG 


QMKLANVV 

« Hide

References

[1]"Cloning and nucleotide sequences of the BanI restriction-modification genes in Bacillus aneurinolyticus."
Maekawa Y., Yasukawa H., Kawakami B.
J. Biochem. 107:645-649(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-15.
Strain: ATCC 12856 / DSM 5562 / JCM 9024 / NBRC 15521 / IAM 1077.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D00704 Genomic DNA. Translation: BAA00613.1.
PIRCTBSBA. JS0489.

3D structure databases

ProteinModelPortalP19888.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3294. M.BanI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTBA_ANEAE
AccessionPrimary (citable) accession number: P19888
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: April 16, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries