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P19888

- MTBA_ANEAE

UniProt

P19888 - MTBA_ANEAE

Protein

Modification methylase BanI

Gene

banIM

Organism
Aneurinibacillus aneurinilyticus (Bacillus aneurinolyticus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GGYRCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the BanI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei76 – 761PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3294. M.BanI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase BanI (EC:2.1.1.37)
    Short name:
    M.BanI
    Alternative name(s):
    Cytosine-specific methyltransferase BanI
    Gene namesi
    Name:banIM
    OrganismiAneurinibacillus aneurinilyticus (Bacillus aneurinolyticus)
    Taxonomic identifieri1391 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeAneurinibacillus groupAneurinibacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 428428Modification methylase BanIPRO_0000087860Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP19888.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 417415SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 2 hits.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P19888-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKIKFVDLFA GIGGIRIGFE RAAKRFELET ECVLSSEIDK KACETYALNF    50
    KEEPQGDIHE ITSFPEFDFL LAGFPCQPFS YAGKQQGFGD TRGTLFFEVE 100
    RVLRDNRPKA FLLENVRGLV THDKGRTLKT IISKLEELGY GVSYLLLNSS 150
    TFGVPQNRVR IYILGILGSK PKLTLTSNVG AADSHKYKNE QISLFDESYA 200
    TVKDILEDSP SEKYRCSDEF IGQLSKVVGN NFELLHGYRL IDYRGGNSIH 250
    SWELGIKGDC TKEEIEFLNQ LIANRRKKIY GTHQDGKALT LEQIRTFYNH 300
    DQLEVIIKSL LQKGYLREEE NKFNPVCGNM SFEVFKFLDP DSISITLTSS 350
    DAHKLGVVQN NVPRRITPRE CARLQGFPDD FILHSNDNFA YKQLGNSVTV 400
    KVVEKVIEDL FQNNVNELFG QMKLANVV 428
    Length:428
    Mass (Da):48,618
    Last modified:February 1, 1991 - v1
    Checksum:iADF3E932A681E43E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00704 Genomic DNA. Translation: BAA00613.1.
    PIRiJS0489. CTBSBA.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00704 Genomic DNA. Translation: BAA00613.1 .
    PIRi JS0489. CTBSBA.

    3D structure databases

    ProteinModelPortali P19888.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3294. M.BanI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 2 hits.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and nucleotide sequences of the BanI restriction-modification genes in Bacillus aneurinolyticus."
      Maekawa Y., Yasukawa H., Kawakami B.
      J. Biochem. 107:645-649(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-15.
      Strain: ATCC 12856 / DSM 5562 / JCM 9024 / NBRC 15521 / IAM 1077.

    Entry informationi

    Entry nameiMTBA_ANEAE
    AccessioniPrimary (citable) accession number: P19888
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3