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P19888

- MTBA_ANEAE

UniProt

P19888 - MTBA_ANEAE

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Protein

Modification methylase BanI

Gene

banIM

Organism
Aneurinibacillus aneurinilyticus (Bacillus aneurinolyticus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

This methylase recognizes the double-stranded sequence GGYRCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the BanI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei76 – 761PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3294. M.BanI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase BanI (EC:2.1.1.37)
Short name:
M.BanI
Alternative name(s):
Cytosine-specific methyltransferase BanI
Gene namesi
Name:banIM
OrganismiAneurinibacillus aneurinilyticus (Bacillus aneurinolyticus)
Taxonomic identifieri1391 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeAneurinibacillus groupAneurinibacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428Modification methylase BanIPRO_0000087860Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP19888.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 417415SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19888-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKIKFVDLFA GIGGIRIGFE RAAKRFELET ECVLSSEIDK KACETYALNF
60 70 80 90 100
KEEPQGDIHE ITSFPEFDFL LAGFPCQPFS YAGKQQGFGD TRGTLFFEVE
110 120 130 140 150
RVLRDNRPKA FLLENVRGLV THDKGRTLKT IISKLEELGY GVSYLLLNSS
160 170 180 190 200
TFGVPQNRVR IYILGILGSK PKLTLTSNVG AADSHKYKNE QISLFDESYA
210 220 230 240 250
TVKDILEDSP SEKYRCSDEF IGQLSKVVGN NFELLHGYRL IDYRGGNSIH
260 270 280 290 300
SWELGIKGDC TKEEIEFLNQ LIANRRKKIY GTHQDGKALT LEQIRTFYNH
310 320 330 340 350
DQLEVIIKSL LQKGYLREEE NKFNPVCGNM SFEVFKFLDP DSISITLTSS
360 370 380 390 400
DAHKLGVVQN NVPRRITPRE CARLQGFPDD FILHSNDNFA YKQLGNSVTV
410 420
KVVEKVIEDL FQNNVNELFG QMKLANVV
Length:428
Mass (Da):48,618
Last modified:February 1, 1991 - v1
Checksum:iADF3E932A681E43E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00704 Genomic DNA. Translation: BAA00613.1.
PIRiJS0489. CTBSBA.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00704 Genomic DNA. Translation: BAA00613.1 .
PIRi JS0489. CTBSBA.

3D structure databases

ProteinModelPortali P19888.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3294. M.BanI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 2 hits.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and nucleotide sequences of the BanI restriction-modification genes in Bacillus aneurinolyticus."
    Maekawa Y., Yasukawa H., Kawakami B.
    J. Biochem. 107:645-649(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-15.
    Strain: ATCC 12856 / DSM 5562 / JCM 9024 / NBRC 15521 / IAM 1077.

Entry informationi

Entry nameiMTBA_ANEAE
AccessioniPrimary (citable) accession number: P19888
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: October 1, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3