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Reviewed, UniProtKB/Swiss-Prot P19887 (T2BA_BACAR)

Last modified July 7, 2009. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Type-2 restriction enzyme BanI
      Short name=R.BanI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme BanI
    Endonuclease BanI
Gene names
Name: banIR
OrganismBacillus aneurinolyticus
Taxonomic identifier1391 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacillalesPaenibacillaceaeAneurinibacillus groupAneurinibacillus

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Recognizes the double-stranded sequence GGYRCC and cleaves after G-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 354353Type-2 restriction enzyme BanI
PRO_0000077282

Sequences

Sequence LengthMass (Da)Tools
P19887-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 8AC3DD73BDAB4B2F

FASTA35439,861
        10         20         30         40         50         60 
MAQLKYNKDI DELERNAAKW WPDFLAKKES STSIIPKLVE SQDAFISLLN LSKNNPFDIF 

        70         80         90        100        110        120 
QLIDASKFPP NLFLKHLVVL TDFGGEPLNR LNQNFDSLFP MIPYGIHYIT KVLGKFEFFW 

       130        140        150        160        170        180 
NEKKYEYVFQ ELPVTSLTNS KLKIDGASIS KTVPLSDLYK DVIVLLMFGA NAVNSEVSEV 

       190        200        210        220        230        240 
LMKCEVGNLI GKTDELKKFI KERYIFVSRI TGGAEANTLG QVAQTHVIDF LRTRFGSKGH 

       250        260        270        280        290        300 
DIKSNGHIEG VTHNDGQTLT TFDVVIKKGS KSVAIEISFQ VTTNSTIERK AGQAKARYDM 

       310        320        330        340        350 
VSDTGNYIAY IIDGAGNFQR KNAITTICNN SHCTVAYTEE ELNVLLKFIL EKLE 

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References

[1]"Cloning and nucleotide sequences of the BanI restriction-modification genes in Bacillus aneurinolyticus."
Maekawa Y., Yasukawa H., Kawakami B.
J. Biochem. 107:645-649(1990) [PubMed: 2358438] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-16.
Strain: IAM 1077.

Cross-references

Sequence databases

D00704 Genomic DNA. Translation: BAA00612.1.
PIRJX0116.

3D structure databases

ModBaseSearch...

Enzyme and pathway databases

BRENDA3.1.21.4. 142380.

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameT2BA_BACAR
AccessionPrimary (citable) accession number: P19887
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: July 7, 2009
This is version 48 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents