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P19887 (T2BA_ANEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme BanI

Short name=R.BanI
EC=3.1.21.4
Alternative name(s):
Endonuclease BanI
Type II restriction enzyme BanI
Gene names
Name:banIR
OrganismAneurinibacillus aneurinilyticus (Bacillus aneurinolyticus)
Taxonomic identifier1391 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeAneurinibacillus groupAneurinibacillus

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded sequence GGYRCC and cleaves after G-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 354353Type-2 restriction enzyme BanI
PRO_0000077282

Sequences

Sequence LengthMass (Da)Tools
P19887 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 8AC3DD73BDAB4B2F

FASTA35439,861
        10         20         30         40         50         60 
MAQLKYNKDI DELERNAAKW WPDFLAKKES STSIIPKLVE SQDAFISLLN LSKNNPFDIF 

        70         80         90        100        110        120 
QLIDASKFPP NLFLKHLVVL TDFGGEPLNR LNQNFDSLFP MIPYGIHYIT KVLGKFEFFW 

       130        140        150        160        170        180 
NEKKYEYVFQ ELPVTSLTNS KLKIDGASIS KTVPLSDLYK DVIVLLMFGA NAVNSEVSEV 

       190        200        210        220        230        240 
LMKCEVGNLI GKTDELKKFI KERYIFVSRI TGGAEANTLG QVAQTHVIDF LRTRFGSKGH 

       250        260        270        280        290        300 
DIKSNGHIEG VTHNDGQTLT TFDVVIKKGS KSVAIEISFQ VTTNSTIERK AGQAKARYDM 

       310        320        330        340        350 
VSDTGNYIAY IIDGAGNFQR KNAITTICNN SHCTVAYTEE ELNVLLKFIL EKLE 

« Hide

References

[1]"Cloning and nucleotide sequences of the BanI restriction-modification genes in Bacillus aneurinolyticus."
Maekawa Y., Yasukawa H., Kawakami B.
J. Biochem. 107:645-649(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-16.
Strain: ATCC 12856 / DSM 5562 / JCM 9024 / NBRC 15521 / IAM 1077.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D00704 Genomic DNA. Translation: BAA00612.1.
PIRJX0116.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE190. BanI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameT2BA_ANEAE
AccessionPrimary (citable) accession number: P19887
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries