P19887 (T2BA_BACAR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme BanI
Short name=R.BanI
EC=3.1.21.4

Alternative name(s):
Endonuclease BanI
Type II restriction enzyme BanI

Gene names

Name:

banIR

Organism

Bacillus aneurinolyticus

Taxonomic identifier

1391 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Paenibacillaceae › Aneurinibacillus group › Aneurinibacillus

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Recognizes the double-stranded sequence GGYRCC and cleaves after G-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 354	353	Type-2 restriction enzyme BanI		PRO_0000077282

Sequences

	Sequence		Length	Mass (Da)
	P19887 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 8AC3DD73BDAB4B2F	FASTA	354	39,861
	10 20 30 40 50 60 MAQLKYNKDI DELERNAAKW WPDFLAKKES STSIIPKLVE SQDAFISLLN LSKNNPFDIF 70 80 90 100 110 120 QLIDASKFPP NLFLKHLVVL TDFGGEPLNR LNQNFDSLFP MIPYGIHYIT KVLGKFEFFW 130 140 150 160 170 180 NEKKYEYVFQ ELPVTSLTNS KLKIDGASIS KTVPLSDLYK DVIVLLMFGA NAVNSEVSEV 190 200 210 220 230 240 LMKCEVGNLI GKTDELKKFI KERYIFVSRI TGGAEANTLG QVAQTHVIDF LRTRFGSKGH 250 260 270 280 290 300 DIKSNGHIEG VTHNDGQTLT TFDVVIKKGS KSVAIEISFQ VTTNSTIERK AGQAKARYDM 310 320 330 340 350 VSDTGNYIAY IIDGAGNFQR KNAITTICNN SHCTVAYTEE ELNVLLKFIL EKLE

References

[1]	"Cloning and nucleotide sequences of the BanI restriction-modification genes in Bacillus aneurinolyticus." Maekawa Y., Yasukawa H., Kawakami B. J. Biochem. 107:645-649(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-16. Strain: IAM 1077.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D00704 Genomic DNA. Translation: BAA00612.1.
PIR	JX0116.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	190. BanI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

T2BA_BACAR

Accession

Primary (citable) accession number: P19887

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program