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Protein

Heat shock protein 60, mitochondrial

Gene

HSP60

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May participate in assembly and/or disassembly of proteins imported into the mitochondrion. HSP60 are ATPases and have affinity for unfolded proteins.

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • chaperone binding Source: SGD
  • DNA replication origin binding Source: SGD
  • single-stranded DNA binding Source: SGD
  • unfolded protein binding Source: SGD

GO - Biological processi

  • 'de novo' protein folding Source: SGD
  • chaperone-mediated protein complex assembly Source: SGD
  • protein import into mitochondrial intermembrane space Source: SGD
  • protein maturation Source: SGD
  • protein refolding Source: SGD
  • protein stabilization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32361-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock protein 60, mitochondrial
Alternative name(s):
CPN60
P66
Stimulator factor I 66 kDa component
Gene namesi
Name:HSP60
Synonyms:MIF4
Ordered Locus Names:YLR259C
ORF Names:L8479.10
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR259C.
SGDiS000004249. HSP60.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • mitochondrial inner membrane Source: Reactome
  • mitochondrial intermembrane space Source: Reactome
  • mitochondrial matrix Source: Reactome
  • mitochondrial nucleoid Source: SGD
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2525MitochondrionSequence analysisAdd
BLAST
Chaini26 – 572547Heat shock protein 60, mitochondrialPRO_0000005044Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei102 – 1021PhosphothreonineCombined sources
Modified residuei485 – 4851PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP19882.
PeptideAtlasiP19882.
PRIDEiP19882.

2D gel databases

SWISS-2DPAGEP19882.
UCD-2DPAGEP19882.

PTM databases

iPTMnetiP19882.

Interactioni

GO - Molecular functioni

  • chaperone binding Source: SGD
  • unfolded protein binding Source: SGD

Protein-protein interaction databases

BioGridi31527. 140 interactions.
DIPiDIP-7648N.
IntActiP19882. 87 interactions.
MINTiMINT-2783335.

Structurei

3D structure databases

ProteinModelPortaliP19882.
SMRiP19882. Positions 23-550.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chaperonin (HSP60) family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000005727.
HOGENOMiHOG000076290.
InParanoidiP19882.
KOiK04077.
OMAiSNAMKKV.
OrthoDBiEOG7KH9TM.

Family and domain databases

Gene3Di1.10.560.10. 2 hits.
3.50.7.10. 1 hit.
HAMAPiMF_00600. CH60.
InterProiIPR018370. Chaperonin_Cpn60_CS.
IPR001844. Chaprnin_Cpn60.
IPR002423. Cpn60/TCP-1.
IPR027409. GroEL-like_apical_dom.
IPR027413. GROEL-like_equatorial.
[Graphical view]
PfamiPF00118. Cpn60_TCP1. 1 hit.
[Graphical view]
PRINTSiPR00298. CHAPERONIN60.
SUPFAMiSSF52029. SSF52029. 1 hit.
TIGRFAMsiTIGR02348. GroEL. 1 hit.
PROSITEiPS00296. CHAPERONINS_CPN60. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19882-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRSSVVRSR ATLRPLLRRA YSSHKELKFG VEGRASLLKG VETLAEAVAA
60 70 80 90 100
TLGPKGRNVL IEQPFGPPKI TKDGVTVAKS IVLKDKFENM GAKLLQEVAS
110 120 130 140 150
KTNEAAGDGT TSATVLGRAI FTESVKNVAA GCNPMDLRRG SQVAVEKVIE
160 170 180 190 200
FLSANKKEIT TSEEIAQVAT ISANGDSHVG KLLASAMEKV GKEGVITIRE
210 220 230 240 250
GRTLEDELEV TEGMRFDRGF ISPYFITDPK SSKVEFEKPL LLLSEKKISS
260 270 280 290 300
IQDILPALEI SNQSRRPLLI IAEDVDGEAL AACILNKLRG QVKVCAVKAP
310 320 330 340 350
GFGDNRKNTI GDIAVLTGGT VFTEELDLKP EQCTIENLGS CDSITVTKED
360 370 380 390 400
TVILNGSGPK EAIQERIEQI KGSIDITTTN SYEKEKLQER LAKLSGGVAV
410 420 430 440 450
IRVGGASEVE VGEKKDRYDD ALNATRAAVE EGILPGGGTA LVKASRVLDE
460 470 480 490 500
VVVDNFDQKL GVDIIRKAIT RPAKQIIENA GEEGSVIIGK LIDEYGDDFA
510 520 530 540 550
KGYDASKSEY TDMLATGIID PFKVVRSGLV DASGVASLLA TTEVAIVDAP
560 570
EPPAAAGAGG MPGGMPGMPG MM
Length:572
Mass (Da):60,752
Last modified:February 1, 1991 - v1
Checksum:i279DC3AF5A2B0A3E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33301 Genomic DNA. Translation: AAA34690.1.
U17244 Genomic DNA. Translation: AAB67380.1.
BK006945 Genomic DNA. Translation: DAA09572.1.
PIRiJQ0157.
RefSeqiNP_013360.1. NM_001182146.1.

Genome annotation databases

EnsemblFungiiYLR259C; YLR259C; YLR259C.
GeneIDi850963.
KEGGisce:YLR259C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33301 Genomic DNA. Translation: AAA34690.1.
U17244 Genomic DNA. Translation: AAB67380.1.
BK006945 Genomic DNA. Translation: DAA09572.1.
PIRiJQ0157.
RefSeqiNP_013360.1. NM_001182146.1.

3D structure databases

ProteinModelPortaliP19882.
SMRiP19882. Positions 23-550.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31527. 140 interactions.
DIPiDIP-7648N.
IntActiP19882. 87 interactions.
MINTiMINT-2783335.

PTM databases

iPTMnetiP19882.

2D gel databases

SWISS-2DPAGEP19882.
UCD-2DPAGEP19882.

Proteomic databases

MaxQBiP19882.
PeptideAtlasiP19882.
PRIDEiP19882.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR259C; YLR259C; YLR259C.
GeneIDi850963.
KEGGisce:YLR259C.

Organism-specific databases

EuPathDBiFungiDB:YLR259C.
SGDiS000004249. HSP60.

Phylogenomic databases

GeneTreeiENSGT00390000005727.
HOGENOMiHOG000076290.
InParanoidiP19882.
KOiK04077.
OMAiSNAMKKV.
OrthoDBiEOG7KH9TM.

Enzyme and pathway databases

BioCyciYEAST:G3O-32361-MONOMER.

Miscellaneous databases

NextBioi967448.
PROiP19882.

Family and domain databases

Gene3Di1.10.560.10. 2 hits.
3.50.7.10. 1 hit.
HAMAPiMF_00600. CH60.
InterProiIPR018370. Chaperonin_Cpn60_CS.
IPR001844. Chaprnin_Cpn60.
IPR002423. Cpn60/TCP-1.
IPR027409. GroEL-like_apical_dom.
IPR027413. GROEL-like_equatorial.
[Graphical view]
PfamiPF00118. Cpn60_TCP1. 1 hit.
[Graphical view]
PRINTSiPR00298. CHAPERONIN60.
SUPFAMiSSF52029. SSF52029. 1 hit.
TIGRFAMsiTIGR02348. GroEL. 1 hit.
PROSITEiPS00296. CHAPERONINS_CPN60. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the yeast chaperonin HSP60 gene."
    Johnson R.B., Fearon K., Mason T., Jindal S.
    Gene 84:295-302(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Characterization of the yeast HSP60 gene coding for a mitochondrial assembly factor."
    Reading D.S., Hallberg R.L., Myers A.M.
    Nature 337:655-659(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 200060 / W303.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The 66 kDa component of yeast SFI, stimulatory factor I, is hsp60."
    Smiley J.K., Brown W.C., Campbell J.L.
    Nucleic Acids Res. 20:4913-4918(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL NUCLEOTIDE SEQUENCE.
  6. "Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
    Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
    Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-34; 101-116; 148-153; 443-450 AND 507-514, SUBCELLULAR LOCATION.
    Strain: ATCC 201238 / W303-1B.
  7. "Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase."
    Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.
    Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-102, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-485, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHSP60_YEAST
AccessioniPrimary (citable) accession number: P19882
Secondary accession number(s): D6VYQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: May 11, 2016
This is version 163 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.