Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

4-nitrophenylphosphatase

Gene

PHO13

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

PHO13 is dispensable for vegetative growth and sporulation.

Catalytic activityi

4-nitrophenyl phosphate + H2O = 4-nitrophenol + phosphate.

Enzyme regulationi

Activity enhanced by Mg2+ ion but inhibited by Ca2+, Zn2+ and Be2+ ions. Inorganic phosphate and ATP are competitive inhibitors, whereas pyrophosphate and AMP have no effect.

GO - Molecular functioni

  • alkaline phosphatase activity Source: SGD
  • phosphoprotein phosphatase activity Source: SGD

GO - Biological processi

  • protein dephosphorylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciYEAST:YDL236W-MONOMER.
BRENDAi3.1.3.18. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
4-nitrophenylphosphatase (EC:3.1.3.41)
Short name:
PNPPase
Gene namesi
Name:PHO13
Ordered Locus Names:YDL236W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL236W.
SGDiS000002395. PHO13.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3123124-nitrophenylphosphatasePRO_0000058481Add
BLAST

Proteomic databases

MaxQBiP19881.
PeptideAtlasiP19881.

PTM databases

iPTMnetiP19881.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi31875. 59 interactions.
DIPiDIP-1665N.
IntActiP19881. 3 interactions.
MINTiMINT-386372.

Structurei

3D structure databases

ProteinModelPortaliP19881.
SMRiP19881. Positions 14-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

GeneTreeiENSGT00510000047020.
HOGENOMiHOG000068104.
InParanoidiP19881.
KOiK01101.
OMAiGMGKSIY.
OrthoDBiEOG7B05Q3.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
3.40.50.10410. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006357. HAD-SF_hydro_IIA.
IPR023215. NPhePase-like_dom.
IPR006349. PGP_euk.
[Graphical view]
PfamiPF13344. Hydrolase_6. 1 hit.
[Graphical view]
PIRSFiPIRSF000915. PGP-type_phosphatase. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01460. HAD-SF-IIA. 1 hit.
TIGR01452. PGP_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

P19881-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAQQGVPIK ITNKEIAQEF LDKYDTFLFD CDGVLWLGSQ ALPYTLEILN
60 70 80 90 100
LLKQLGKQLI FVTNNSTKSR LAYTKKFASF GIDVKEEQIF TSGYASAVYI
110 120 130 140 150
RDFLKLQPGK DKVWVFGESG IGEELKLMGY ESLGGADSRL DTPFDAAKSP
160 170 180 190 200
FLVNGLDKDV SCVIAGLDTK VNYHRLAVTL QYLQKDSVHF VGTNVDSTFP
210 220 230 240 250
QKGYTFPGAG SMIESLAFSS NRRPSYCGKP NQNMLNSIIS AFNLDRSKCC
260 270 280 290 300
MVGDRLNTDM KFGVEGGLGG TLLVLSGIET EERALKISHD YPRPKFYIDK
310
LGDIYTLTNN EL
Length:312
Mass (Da):34,625
Last modified:December 21, 2004 - v2
Checksum:iBBF36D5547018CD5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501N → D in CAB56540 (PubMed:2558283).Curated
Sequence conflicti96 – 961S → P in CAB56540 (PubMed:2558283).Curated
Sequence conflicti284 – 2841A → T in CAB56540 (PubMed:2558283).Curated
Sequence conflicti306 – 3061T → A in CAB56540 (PubMed:2558283).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51611 Genomic DNA. Translation: CAB56540.1.
Z74284 Genomic DNA. Translation: CAA98816.1.
BK006938 Genomic DNA. Translation: DAA11630.1.
PIRiS67800.
RefSeqiNP_010045.1. NM_001180296.1.

Genome annotation databases

EnsemblFungiiYDL236W; YDL236W; YDL236W.
GeneIDi851362.
KEGGisce:YDL236W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51611 Genomic DNA. Translation: CAB56540.1.
Z74284 Genomic DNA. Translation: CAA98816.1.
BK006938 Genomic DNA. Translation: DAA11630.1.
PIRiS67800.
RefSeqiNP_010045.1. NM_001180296.1.

3D structure databases

ProteinModelPortaliP19881.
SMRiP19881. Positions 14-311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31875. 59 interactions.
DIPiDIP-1665N.
IntActiP19881. 3 interactions.
MINTiMINT-386372.

PTM databases

iPTMnetiP19881.

Proteomic databases

MaxQBiP19881.
PeptideAtlasiP19881.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL236W; YDL236W; YDL236W.
GeneIDi851362.
KEGGisce:YDL236W.

Organism-specific databases

EuPathDBiFungiDB:YDL236W.
SGDiS000002395. PHO13.

Phylogenomic databases

GeneTreeiENSGT00510000047020.
HOGENOMiHOG000068104.
InParanoidiP19881.
KOiK01101.
OMAiGMGKSIY.
OrthoDBiEOG7B05Q3.

Enzyme and pathway databases

BioCyciYEAST:YDL236W-MONOMER.
BRENDAi3.1.3.18. 984.

Miscellaneous databases

NextBioi968468.
PROiP19881.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
3.40.50.10410. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006357. HAD-SF_hydro_IIA.
IPR023215. NPhePase-like_dom.
IPR006349. PGP_euk.
[Graphical view]
PfamiPF13344. Hydrolase_6. 1 hit.
[Graphical view]
PIRSFiPIRSF000915. PGP-type_phosphatase. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01460. HAD-SF-IIA. 1 hit.
TIGR01452. PGP_euk. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of a specific p-nitrophenylphosphatase gene, PHO13, and its mapping by chromosome fragmentation in Saccharomyces cerevisiae."
    Kaneko Y., Toh-e A., Banno I., Oshima Y.
    Mol. Gen. Genet. 220:133-139(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPNPP_YEAST
AccessioniPrimary (citable) accession number: P19881
Secondary accession number(s): D6VRC0, Q07689
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: December 21, 2004
Last modified: May 11, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2980 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.