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Protein

4-nitrophenylphosphatase

Gene

PHO13

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

PHO13 is dispensable for vegetative growth and sporulation.

Miscellaneous

Present with 2980 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

4-nitrophenyl phosphate + H2O = 4-nitrophenol + phosphate.

Enzyme regulationi

Activity enhanced by Mg2+ ion but inhibited by Ca2+, Zn2+ and Be2+ ions. Inorganic phosphate and ATP are competitive inhibitors, whereas pyrophosphate and AMP have no effect.

GO - Molecular functioni

  • alkaline phosphatase activity Source: SGD
  • phosphatase activity Source: SGD
  • phosphoglycolate phosphatase activity Source: SGD
  • phosphoprotein phosphatase activity Source: SGD

GO - Biological processi

  • 4-nitrophenol catabolic process Source: GO_Central
  • phosphorylated carbohydrate dephosphorylation Source: SGD
  • protein dephosphorylation Source: SGD

Keywordsi

Molecular functionHydrolase
LigandMagnesium

Enzyme and pathway databases

BioCyciYEAST:YDL236W-MONOMER
BRENDAi3.1.3.18 984

Names & Taxonomyi

Protein namesi
Recommended name:
4-nitrophenylphosphatase (EC:3.1.3.41)
Short name:
PNPPase
Gene namesi
Name:PHO13
Ordered Locus Names:YDL236W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL236W
SGDiS000002395 PHO13

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000584811 – 3124-nitrophenylphosphataseAdd BLAST312

Proteomic databases

MaxQBiP19881
PaxDbiP19881
PRIDEiP19881

PTM databases

iPTMnetiP19881

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi31875, 88 interactors
DIPiDIP-1665N
IntActiP19881, 8 interactors
MINTiP19881
STRINGi4932.YDL236W

Structurei

3D structure databases

ProteinModelPortaliP19881
SMRiP19881
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

GeneTreeiENSGT00510000047020
HOGENOMiHOG000068104
InParanoidiP19881
KOiK01101
OMAiVFGTAYC
OrthoDBiEOG092C2TLF

Family and domain databases

Gene3Di3.40.50.1000, 3 hits
InterProiView protein in InterPro
IPR036412 HAD-like_sf
IPR006357 HAD-SF_hydro_IIA
IPR023214 HAD_sf
IPR006349 PGP_euk
PfamiView protein in Pfam
PF13344 Hydrolase_6, 1 hit
PIRSFiPIRSF000915 PGP-type_phosphatase, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR01460 HAD-SF-IIA, 1 hit
TIGR01452 PGP_euk, 1 hit

Sequencei

Sequence statusi: Complete.

P19881-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAQQGVPIK ITNKEIAQEF LDKYDTFLFD CDGVLWLGSQ ALPYTLEILN
60 70 80 90 100
LLKQLGKQLI FVTNNSTKSR LAYTKKFASF GIDVKEEQIF TSGYASAVYI
110 120 130 140 150
RDFLKLQPGK DKVWVFGESG IGEELKLMGY ESLGGADSRL DTPFDAAKSP
160 170 180 190 200
FLVNGLDKDV SCVIAGLDTK VNYHRLAVTL QYLQKDSVHF VGTNVDSTFP
210 220 230 240 250
QKGYTFPGAG SMIESLAFSS NRRPSYCGKP NQNMLNSIIS AFNLDRSKCC
260 270 280 290 300
MVGDRLNTDM KFGVEGGLGG TLLVLSGIET EERALKISHD YPRPKFYIDK
310
LGDIYTLTNN EL
Length:312
Mass (Da):34,625
Last modified:December 21, 2004 - v2
Checksum:iBBF36D5547018CD5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti50N → D in CAB56540 (PubMed:2558283).Curated1
Sequence conflicti96S → P in CAB56540 (PubMed:2558283).Curated1
Sequence conflicti284A → T in CAB56540 (PubMed:2558283).Curated1
Sequence conflicti306T → A in CAB56540 (PubMed:2558283).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51611 Genomic DNA Translation: CAB56540.1
Z74284 Genomic DNA Translation: CAA98816.1
BK006938 Genomic DNA Translation: DAA11630.1
PIRiS67800
RefSeqiNP_010045.1, NM_001180296.1

Genome annotation databases

EnsemblFungiiYDL236W; YDL236W; YDL236W
GeneIDi851362
KEGGisce:YDL236W

Similar proteinsi

Entry informationi

Entry nameiPNPP_YEAST
AccessioniPrimary (citable) accession number: P19881
Secondary accession number(s): D6VRC0, Q07689
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: December 21, 2004
Last modified: March 28, 2018
This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health