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Reviewed, UniProtKB/Swiss-Prot P19881 (PNPP_YEAST)

Last modified June 16, 2009. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    4-nitrophenylphosphatase
      Short name=PNPPase
    EC=3.1.3.41
Gene names
Name: PHO13
Ordered Locus Names: YDL236W
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length312 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

PHO13 is dispensable for vegetative growth and sporulation.

Catalytic activity

4-nitrophenyl phosphate + H2O = 4-nitrophenol + phosphate.

Enzyme regulation

Activity enhanced by Mg2+ ion but inhibited by Ca2+, Zn2+ and Be2+ ions. Inorganic phosphate and ATP are competitive inhibitors, whereas pyrophosphate and AMP have no effect.

Subunit structure

Homodimer.

Miscellaneous

Present with 2980 molecules/cell in log phase SD medium. Ref.3

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3123124-nitrophenylphosphatase
PRO_0000058481

Amino acid modifications

Modified residue1971Phosphoserine Ref.5
Modified residue1981Phosphothreonine Ref.4

Experimental info

Sequence conflict501N → D in CAB56540. Ref.1
Sequence conflict961S → P in CAB56540. Ref.1
Sequence conflict2841A → T in CAB56540. Ref.1
Sequence conflict3061T → A in CAB56540. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P19881-1 [UniParc].

Last modified December 21, 2004. Version 2.
Checksum: BBF36D5547018CD5

FASTA31234,625
        10         20         30         40         50         60 
MTAQQGVPIK ITNKEIAQEF LDKYDTFLFD CDGVLWLGSQ ALPYTLEILN LLKQLGKQLI 

        70         80         90        100        110        120 
FVTNNSTKSR LAYTKKFASF GIDVKEEQIF TSGYASAVYI RDFLKLQPGK DKVWVFGESG 

       130        140        150        160        170        180 
IGEELKLMGY ESLGGADSRL DTPFDAAKSP FLVNGLDKDV SCVIAGLDTK VNYHRLAVTL 

       190        200        210        220        230        240 
QYLQKDSVHF VGTNVDSTFP QKGYTFPGAG SMIESLAFSS NRRPSYCGKP NQNMLNSIIS 

       250        260        270        280        290        300 
AFNLDRSKCC MVGDRLNTDM KFGVEGGLGG TLLVLSGIET EERALKISHD YPRPKFYIDK 

       310 
LGDIYTLTNN EL

« Hide

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References

« Hide 'large scale' references
[1]"Molecular characterization of a specific p-nitrophenylphosphatase gene, PHO13, and its mapping by chromosome fragmentation in Saccharomyces cerevisiae."
Kaneko Y., Toh-e A., Banno I., Oshima Y.
Mol. Gen. Genet. 220:133-139(1989) [PubMed: 2558283] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed: 9169867] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-198, MASS SPECTROMETRY.
[5]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-197, MASS SPECTROMETRY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

X51611 Genomic DNA. Translation: CAB56540.1.
Z74284 Genomic DNA. Translation: CAA98816.1.
PIRS67800.
RefSeqNP_010045.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:1665N.
IntActP19881. 10 interactions.

Proteomic databases

PeptideAtlasP19881.

Genome annotation databases

EnsemblYDL236W. Saccharomyces cerevisiae. [Contig view]
GeneID851362.
GenomeReviewsGene locus YDL236W in contig Z71256_GR.
KEGGsce:YDL236W.
NMPDRfig|4932.3.peg.778.

Organism-specific databases

CYGDYDL236w.
SGDS000002395. PHO13.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP19881.
OMAP19881. WNGERAV.

Enzyme and pathway databases

BRENDA3.1.3.18. 250.
3.1.3.41. 250.

Gene expression databases

GermOnlineYDL236W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR005834. Dehalogen-like_hydro.
IPR006357. HAD-SF_hydro_IIA.
IPR006349. PGP_euk.
[Graphical view]
PfamPF00702. Hydrolase. 1 hit.
[Graphical view]
TIGRFAMsTIGR01460. HAD-SF-IIA. 1 hit.
TIGR01452. PGP_euk. 1 hit.
ProtoNetSearch...

Other Resources

NextBio968468.

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Entry information

Entry namePNPP_YEAST
AccessionPrimary (citable) accession number: P19881
Secondary accession number(s): Q07689
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: December 21, 2004
Last modified: June 16, 2009
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents