Reviewed, UniProtKB/Swiss-Prot P19871 (3BHD_COMTE)

Names and origin

Protein names	Recommended name:     3-beta-hydroxysteroid dehydrogenase     EC=1.1.1.51
Organism	Comamonas testosteroni (Pseudomonas testosteroni)
Taxonomic identifier	285 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Comamonas

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Testosterone + NAD(P)+ = androst-4-ene-3,17-dione + NAD(P)H.
Subunit structure	Homotetramer. Ref.4
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Lipid metabolism Steroid metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3(or 17)beta-hydroxysteroid dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.3

Chain

2 – 254

253

3-beta-hydroxysteroid dehydrogenase

PRO_0000054446

Regions

Nucleotide binding

12 – 40

29

NAD By similarity

Sites

Active site

152

1

Proton acceptor By similarity

Binding site

61

1

NAD By similarity

Binding site

139

1

Substrate By similarity

Binding site

156

1

NAD By similarity

Experimental info

Sequence conflict

14 – 15

2

GG → VV in CAA44977. Ref.1

Sequence conflict

41

1

Missing in CAA44977. Ref.1

Sequence conflict

178

1

R → RR in CAA44977. Ref.1

Sequence conflict

241

1

S → G AA sequence Ref.3

Secondary structure

1

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254

Helix Strand Turn

Details...

Turn

4 – 7

4

Beta strand

9 – 12

4

Turn

13 – 16

4

Helix

18 – 29

12

Beta strand

33 – 37

5

Helix

41 – 51

11

Beta strand

55 – 58

4

Helix

65 – 79

15

Beta strand

84 – 87

4

Turn

97 – 99

3

Helix

102 – 112

11

Helix

114 – 127

14

Turn

128 – 130

3

Beta strand

132 – 137

6

Helix

140 – 142

3

Helix

150 – 173

24

Beta strand

177 – 187

11

Helix

190 – 195

6

Helix

202 – 205

4

Turn

209 – 211

3

Helix

220 – 231

12

Helix

233 – 235

3

Beta strand

242 – 248

7

Turn

250 – 253

4

Sequences

	Sequence		Length	Mass (Da)
	P19871-1 [UniParc]. Last modified January 23, 2007. Version 5. Checksum: FB6EC90B151975DB	FASTA	254	26,952
	10 20 30 40 50 60 MTNRLQGKVA LVTGGASGVG LEVVKLLLGE GAKVAFSDIN EAAGQQLAAE LGERSMFVRH 70 80 90 100 110 120 DVSSEADWTL VMAAVQRRLG TLNVLVNNAG ILLPGDMETG RLEDFSRLLK INTESVFIGC 130 140 150 160 170 180 QQGIAAMKET GGSIINMASV SSWLPIEQYA GYSASKAAVS ALTRAAALSC RKQGYAIRVN 190 200 210 220 230 240 SIHPDGIYTP MMQASLPKGV SKEMVLHDPK LNRAGRAYMP ERIAQLVLFL ASDESSVMSG 250 SELHADNSIL GMGL

References

[1]	"Cloning, DNA sequencing and expression of (3-17)beta hydroxysteroid dehydrogenase from Pseudomonas testosteroni." Abalain J.H., di Stefano S., Amet Y., Quemener E., Abalain-Colloc M.L., Floch H.H. J. Steroid Biochem. Mol. Biol. 44:133-139(1993) [PubMed: 8382516] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 11996 / DSM 50244 / JCM 5832 / NCIB 8955 / NCTC 10698.
[2]	"Identification of a novel steroid inducible gene associated with the beta hsd locus of Comamonas testosteroni." Pruneda-Paz J.L., Linares M., Cabrera J.E., Genti-Raimondi S. J. Steroid Biochem. Mol. Biol. 88:91-100(2004) [PubMed: 15026087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 11996 / DSM 50244 / JCM 5832 / NCIB 8955 / NCTC 10698.
[3]	"Pseudomonas 3 beta-hydroxysteroid dehydrogenase. Primary structure and relationships to other steroid dehydrogenases." Yin S.-J., Vagelopoulos N., Lundquist G., Joernvall H. Eur. J. Biochem. 197:359-365(1991) [PubMed: 2026158] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-254.
[4]	"Crystallization and crystal packing of recombinant 3 (or 17) beta-hydroxysteroid dehydrogenase from Comamonas testosteroni ATTC 11996." Benach J., Knapp S., Oppermann U.C.T., Haegllund O., Joernvall H., Ladenstein R. Eur. J. Biochem. 236:144-148(1996) [PubMed: 8617258] [Abstract] Cited for: CRYSTALLIZATION, SUBUNIT. Strain: ATCC 11996 / DSM 50244 / JCM 5832 / NCIB 8955 / NCTC 10698.
[5]	"Structure of bacterial 3beta/17beta-hydroxysteroid dehydrogenase at 1.2 A resolution: a model for multiple steroid recognition." Benach J., Filling C., Oppermann U.C.T., Roversi P., Bricogne G., Berndt K.D., Joernvall H., Ladenstein R. Biochemistry 41:14659-14668(2002) [PubMed: 12475215] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.22 ANGSTROMS).

Web resources

Worthington enzyme manual

Cross-references

Sequence databases

EMBLGenBankDDBJ

X63379 Genomic DNA. Translation: CAA44977.1.
U41265 Genomic DNA. Translation: AAA25742.1.

PIR

S48129.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1HXH	X-ray	1.22	A/B/C/D	2-254	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

1.1.1.51. 4566.

Family and domain databases

InterPro

IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

PANTHER

PTHR19410. ADH_short_C2. 1 hit.

Pfam

PF00106. adh_short. 1 hit.
[Graphical view]

PRINTS

PR00081. GDHRDH.
PR00080. SDRFAMILY.

PROSITE

PS00061. ADH_SHORT. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

3BHD_COMTE

Accession

Primary (citable) accession number: P19871
Secondary accession number(s): Q52587

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 71 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)