Reviewed, UniProtKB/Swiss-Prot P19870 (INU2_ARTGO)

Names and origin

Protein names	Recommended name:     Inulin fructotransferase [DFA-I-forming]     EC=4.2.2.17 Alternative name(s):     Inulin fructotransferase [depolymerizing, difructofuranose-1,2':2',1-dianhydride-forming]
Organism	Arthrobacter globiformis
Taxonomic identifier	1665 [NCBI]
Taxonomic lineage	Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity

Produces alpha-D-fructofuranose beta-D-fructofuranose 1,2':2,1'-dianhydride (DFA I) by successively eliminating the diminishing (2->1)-beta-D-fructan (inulin) chain from the terminal D-fructosyl-D-fructosyl disaccharide.

Ontologies

Keywords
Molecular function	Glycosyltransferase Lyase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	inulin fructotransferase (DFA-I-forming) activity Inferred from electronic annotation. Source: EC transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1 Ref.2
	Chain	2 – 393	392	Inulin fructotransferase [DFA-I-forming]		PRO_0000084209

Sequences

	Sequence		Length	Mass (Da)
	P19870-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 2F1F15373734BEA9	FASTA	393	41,683
	10 20 30 40 50 60 MANTVYDVTT WSGATISPYV DIGAVINQII ADIKANQTSQ AARPGAVIYI PPGHYDLLTR 70 80 90 100 110 120 VVVDVSFLQI KGSGHGFLSE AIRDESSTGS WVETQPGASH IRVKNTDGNR EAFLVSRSGD 130 140 150 160 170 180 PNVVGRLNSI EFKGFCLDGV TDSKPYSPGN SKIGISVQSD NDSFHVEGMG FVYLEHAIIV 190 200 210 220 230 240 KGADAPNITN NFIAECGSCI ELTGASQVAK ITNNFLISAW AGYSIYAENA EGPLITGNSL 250 260 270 280 290 300 LWAANITLSD CNRVSISSNK LLSNFPSMVA LLGNCSENLI AANHFRRVSG DGTSTRFDDL 310 320 330 340 350 360 FGLVHIEGNN NTVTGNMFSF NVPASSISPS GATPTIILVK SGDSNYLATN NIVSNVSAMV 370 380 390 VLDGSTTATR IIYSAKNSQL NAYTTSYTLV PTP

References

[1]	"Cloning and nucleotide sequence of the inulin fructotransferase (DFA I-producing) gene of Arthrobacter globiformis S14-3." Haraguchi K., Seki K., Kishimoto M., Nagata T., Kasumi T., Kainuma K., Kobayashi S. Biosci. Biotechnol. Biochem. 59:1809-1812(1995) [PubMed: 8534968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21 AND 341-265. Strain: S14-3.
[2]	"Purification and properties of a novel inulin fructotransferase (DFA I-producing) from Arthrobacter globiformis S14-3." Seki K., Haraguchi K., Kishimoto M., Kobayashi S., Kainuma K. Agric. Biol. Chem. 53:2089-2094(1989) Cited for: PROTEIN SEQUENCE OF 2-21. Strain: S14-3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D38528 Genomic DNA. Translation: BAA07533.1.
PIR	JC4318.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	PL19. Polysaccharide Lyase Family 19.
Enzyme and pathway databases
BRENDA	4.2.2.17. 1012.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

INU2_ARTGO

Accession

Primary (citable) accession number: P19870

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	January 23, 2007
Last modified:	April 14, 2009
This is version 39 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)