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Protein

Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic

Gene

GAPA

Organism
Spinacia oleracea (Spinach)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NADP+ = 3-phospho-D-glyceroyl phosphate + NADPH.

Pathwayi: Calvin cycle

This protein is involved in the pathway Calvin cycle, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway Calvin cycle and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100NADP4 Publications1
Binding sitei145NADP; via carbonyl oxygen4 Publications1
Active sitei218Nucleophile1
Sitei245Activates thiol group during catalysisBy similarity1
Binding sitei248Glyceraldehyde 3-phosphateBy similarity1
Binding sitei263Glyceraldehyde 3-phosphateBy similarity1
Binding sitei299Glyceraldehyde 3-phosphateBy similarity1
Binding sitei381NADP4 Publications1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi76 – 77NADP4 Publications2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Calvin cycle

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.2.1.13. 5812.
SABIO-RKP19866.
UniPathwayiUPA00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic (EC:1.2.1.13)
Alternative name(s):
NADP-dependent glyceraldehydephosphate dehydrogenase subunit A
Gene namesi
Name:GAPA
Synonyms:GPA1
OrganismiSpinacia oleracea (Spinach)
Taxonomic identifieri3562 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesChenopodiaceaeChenopodioideaeAnserineaeSpinacia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi101T → A: Reduced affinity for NADP, and slight increase of the affinity for NAD. 1 Publication1
Mutagenesisi257S → A: Reduced affinity for NADP, and slight increase of the affinity for NAD. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 65Chloroplast1 PublicationAdd BLAST65
ChainiPRO_000001042566 – 401Glyceraldehyde-3-phosphate dehydrogenase A, chloroplasticAdd BLAST336

Proteomic databases

PRIDEiP19866.

Interactioni

Subunit structurei

Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1).4 Publications

Protein-protein interaction databases

DIPiDIP-60959N.

Structurei

Secondary structure

1401
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi67 – 72Combined sources6
Helixi76 – 86Combined sources11
Beta strandi88 – 90Combined sources3
Beta strandi92 – 99Combined sources8
Helixi104 – 112Combined sources9
Turni115 – 117Combined sources3
Beta strandi124 – 126Combined sources3
Beta strandi130 – 134Combined sources5
Beta strandi137 – 142Combined sources6
Helixi147 – 149Combined sources3
Helixi152 – 155Combined sources4
Beta strandi159 – 162Combined sources4
Beta strandi164 – 166Combined sources3
Helixi170 – 178Combined sources9
Beta strandi182 – 188Combined sources7
Beta strandi191 – 193Combined sources3
Turni199 – 201Combined sources3
Helixi203 – 205Combined sources3
Beta strandi211 – 214Combined sources4
Helixi218 – 234Combined sources17
Beta strandi236 – 246Combined sources11
Beta strandi253 – 255Combined sources3
Turni261 – 264Combined sources4
Turni267 – 269Combined sources3
Beta strandi272 – 274Combined sources3
Helixi278 – 285Combined sources8
Helixi287 – 289Combined sources3
Turni290 – 292Combined sources3
Beta strandi293 – 301Combined sources9
Beta strandi306 – 316Combined sources11
Helixi320 – 331Combined sources12
Turni332 – 338Combined sources7
Beta strandi339 – 342Combined sources4
Helixi348 – 351Combined sources4
Beta strandi356 – 361Combined sources6
Helixi362 – 364Combined sources3
Beta strandi366 – 368Combined sources3
Turni369 – 371Combined sources3
Beta strandi372 – 379Combined sources8
Helixi383 – 398Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JN0X-ray3.00A/B/O66-401[»]
1NBOX-ray2.60A/B/O66-401[»]
1RM3X-ray2.20A/B/O66-401[»]
1RM4X-ray2.00A/B/O66-401[»]
1RM5X-ray2.10A/B/O66-401[»]
2HKIX-ray3.00A66-401[»]
2PKQX-ray3.60P/R/S66-401[»]
2PKRX-ray2.40A/B/C/D/H/I/L/M/O/P/Q/R66-401[»]
ProteinModelPortaliP19866.
SMRiP19866.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP19866.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni217 – 219Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni276 – 277Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19866-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASNMLSIAN PSLRVYNKGF SEFSGLHTSS LPFGRKGSDD LMAFVSFQTN
60 70 80 90 100
AVGGKRSSQN GVVEAKLKVA INGFGRIGRN FLRCWHGRKD SPLDVVVIND
110 120 130 140 150
TGGVKQASHL LKYDSILGTF DADVKTAGDS AISVDGKVIK VVSDRNPVNL
160 170 180 190 200
PWGDMGIDLV IEGTGVFVDR DGAGKHLQAG AKKVLITAPG KGDIPTYVVG
210 220 230 240 250
VNEEGYTHAD TIISNASCTT NCLAPFVKVL DQKFGIIKGT MTTTHSYTGD
260 270 280 290 300
QRLLDASHRD LRRARAACLN IVPTSTGAAK AVALVLPNLK GKLNGIALRV
310 320 330 340 350
PTPNVSVVDL VVQVSKKTFA EEVNAAFRES ADNELKGILS VCDEPLVSID
360 370 380 390 400
FRCTDVSSTI DSSLTMVMGD DMVKVIAWYD NEWGYSQRVV DLADIVANKW

Q
Length:401
Mass (Da):43,023
Last modified:May 2, 2002 - v2
Checksum:i2A815842EA095A84
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti288N → Q AA sequence (PubMed:2223845).Curated1
Sequence conflicti401Q → QA AA sequence (PubMed:2223845).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L76552 mRNA. Translation: AAD10217.1.
PIRiT09012.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L76552 mRNA. Translation: AAD10217.1.
PIRiT09012.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JN0X-ray3.00A/B/O66-401[»]
1NBOX-ray2.60A/B/O66-401[»]
1RM3X-ray2.20A/B/O66-401[»]
1RM4X-ray2.00A/B/O66-401[»]
1RM5X-ray2.10A/B/O66-401[»]
2HKIX-ray3.00A66-401[»]
2PKQX-ray3.60P/R/S66-401[»]
2PKRX-ray2.40A/B/C/D/H/I/L/M/O/P/Q/R66-401[»]
ProteinModelPortaliP19866.
SMRiP19866.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60959N.

Proteomic databases

PRIDEiP19866.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00116.
BRENDAi1.2.1.13. 5812.
SABIO-RKP19866.

Miscellaneous databases

EvolutionaryTraceiP19866.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3PA_SPIOL
AccessioniPrimary (citable) accession number: P19866
Secondary accession number(s): O20249
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: May 2, 2002
Last modified: November 2, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants contain two types of GAPDH: cytosolic forms which participate in glycolysis and chloroplast forms which participate in photosynthesis. All the forms are encoded by distinct genes.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.