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Protein

L-lactate dehydrogenase A chain

Gene

LDHA

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase A-like 6B (LDHAL6B), L-lactate dehydrogenase A chain (LDHA), L-lactate dehydrogenase B chain (LDHB), L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase (LDHB), L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase (LDHC)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei106 – 1061SubstrateBy similarity
Binding sitei138 – 1381NAD or substrateBy similarity
Binding sitei169 – 1691SubstrateBy similarity
Active sitei193 – 1931Proton acceptorBy similarity
Binding sitei248 – 2481SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 5729NADBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-BTA-70268. Pyruvate metabolism.
SABIO-RKP19858.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase A chain (EC:1.1.1.27)
Short name:
LDH-A
Alternative name(s):
LDH muscle subunit
Short name:
LDH-M
Gene namesi
Name:LDHA
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 29

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Protein family/group databases

Allergomei11906. Bos d LD.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 332331L-lactate dehydrogenase A chainPRO_0000168410Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei5 – 51N6-acetyllysine; alternateBy similarity
Modified residuei5 – 51N6-succinyllysine; alternateBy similarity
Modified residuei14 – 141N6-acetyllysineBy similarity
Modified residuei57 – 571N6-acetyllysineBy similarity
Modified residuei81 – 811N6-acetyllysineBy similarity
Modified residuei118 – 1181N6-acetyllysine; alternateBy similarity
Modified residuei118 – 1181N6-succinyllysine; alternateBy similarity
Modified residuei126 – 1261N6-acetyllysineBy similarity
Modified residuei224 – 2241N6-acetyllysineBy similarity
Modified residuei232 – 2321N6-acetyllysineBy similarity
Modified residuei239 – 2391PhosphotyrosineBy similarity
Modified residuei243 – 2431N6-acetyllysineBy similarity
Modified residuei309 – 3091PhosphothreonineBy similarity
Modified residuei318 – 3181N6-acetyllysine; alternateBy similarity
Modified residuei318 – 3181N6-succinyllysine; alternateBy similarity
Modified residuei322 – 3221PhosphothreonineBy similarity

Post-translational modificationi

ISGylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP19858.
PRIDEiP19858.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

IntActiP19858. 1 interaction.
STRINGi9913.ENSBTAP00000011447.

Structurei

3D structure databases

ProteinModelPortaliP19858.
SMRiP19858. Positions 2-332.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.
GeneTreeiENSGT00550000074541.
HOGENOMiHOG000213793.
HOVERGENiHBG000462.
InParanoidiP19858.
KOiK00016.
OMAiAGDYEDC.
OrthoDBiEOG7X0VH3.
TreeFamiTF314963.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19858-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLKDQLIQ NLLKEEHVPQ NKITIVGVGA VGMACAISIL MKDLADEVAL
60 70 80 90 100
VDVMEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSR LVIITAGARQ
110 120 130 140 150
QEGESRLNLV QRNVNIFKFI IPNIVKYSPN CKLLVVSNPV DILTYVAWKI
160 170 180 190 200
SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWIL GEHGDSSVPV
210 220 230 240 250
WSGVNVAGVS LKNLHPELGT DADKEQWKAV HKQVVDSAYE VIKLKGYTSW
260 270 280 290 300
AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGIKEDVFLS VPCILGQNGI
310 320 330
SDVVKVTLTH EEEACLKKSA DTLWGIQKEL QF
Length:332
Mass (Da):36,598
Last modified:January 23, 2007 - v2
Checksum:iDAC59F02BBA7D16C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90141 mRNA. Translation: BAA14169.1.
D90142 mRNA. Translation: BAA14170.1.
D90143 mRNA. Translation: BAA14171.1.
BC146210 mRNA. Translation: AAI46211.1.
PIRiJQ2222.
RefSeqiNP_776524.1. NM_174099.2.
XP_005226796.1. XM_005226739.2.
UniGeneiBt.3809.

Genome annotation databases

EnsembliENSBTAT00000011447; ENSBTAP00000011447; ENSBTAG00000008683.
ENSBTAT00000056276; ENSBTAP00000047729; ENSBTAG00000008683.
GeneIDi281274.
KEGGibta:281274.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90141 mRNA. Translation: BAA14169.1.
D90142 mRNA. Translation: BAA14170.1.
D90143 mRNA. Translation: BAA14171.1.
BC146210 mRNA. Translation: AAI46211.1.
PIRiJQ2222.
RefSeqiNP_776524.1. NM_174099.2.
XP_005226796.1. XM_005226739.2.
UniGeneiBt.3809.

3D structure databases

ProteinModelPortaliP19858.
SMRiP19858. Positions 2-332.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP19858. 1 interaction.
STRINGi9913.ENSBTAP00000011447.

Protein family/group databases

Allergomei11906. Bos d LD.

Proteomic databases

PaxDbiP19858.
PRIDEiP19858.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000011447; ENSBTAP00000011447; ENSBTAG00000008683.
ENSBTAT00000056276; ENSBTAP00000047729; ENSBTAG00000008683.
GeneIDi281274.
KEGGibta:281274.

Organism-specific databases

CTDi3939.

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.
GeneTreeiENSGT00550000074541.
HOGENOMiHOG000213793.
HOVERGENiHBG000462.
InParanoidiP19858.
KOiK00016.
OMAiAGDYEDC.
OrthoDBiEOG7X0VH3.
TreeFamiTF314963.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.
ReactomeiR-BTA-70268. Pyruvate metabolism.
SABIO-RKP19858.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure of bovine lactate dehydrogenase-A isozyme and its synthesis in Escherichia coli."
    Ishiguro N., Osame S., Kagiya R., Ichijo S., Shinagawa M.
    Gene 91:281-285(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal skin.

Entry informationi

Entry nameiLDHA_BOVIN
AccessioniPrimary (citable) accession number: P19858
Secondary accession number(s): A6H7E0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.