P19840 (LUXB1_PHOLU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alkanal monooxygenase beta chain
EC=1.14.14.3

Alternative name(s):
Bacterial luciferase beta chain

Gene names

Name:

luxB

Organism

Photorhabdus luminescens (Xenorhabdus luminescens)

Taxonomic identifier

29488 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Light-emitting reaction in luminous bacteria. The specific role of the beta subunit is unknown, but it is absolutely required for bioluminescence activity.
Catalytic activity	RCHO + reduced FMN + O2 = RCOOH + FMN + H2O + light.
Subunit structure	Heterodimer of an alpha and a beta chain.
Sequence similarities	Belongs to the bacterial luciferase oxidoreductase family.

Ontologies

Keywords
Biological process	Luminescence
Ligand	FMN Flavoprotein
Molecular function	Monooxygenase Oxidoreductase Photoprotein
Gene Ontology (GO)
Biological_process	bioluminescence Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	alkanal monooxygenase (FMN-linked) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Alkanal monooxygenase beta chain		PRO_0000220177
Natural variations
	Natural variant	85	1	A → R in strain: Hm.

Sequences

	Sequence		Length	Mass (Da)
	P19840 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 1905AF01658BE56E	FASTA	327	37,595
	10 20 30 40 50 60 MKFGLFFLNF INSTTVQEQS IVRMQEITEY VDKLNFEQIL VYENHFSDNG VVGAPLTVSG 70 80 90 100 110 120 FLLGLTEKIK IGSLNHIITT HHPVAIAEEA CLLDQLSEGR FILGFSDCEK KDEMHFFNRP 130 140 150 160 170 180 VEYQQQLFEE CYEIINDALT TGYCNPDNDF YSFPKISVNP HAYTPGGPRK YVTATSHHIV 190 200 210 220 230 240 EWAAKKGIPL IFKWDDSNDV RYEYAERYKA VADKYDVDLS EIDHQLMILV NYNEDSNKAK 250 260 270 280 290 300 QETRAFISDY VLEMHPNENF ENKLEEIIAE NAVGNYTECI TAAKLAIEKC GAKSVLLSFE 310 320 PMNDLMSQKN VINIVDDNIK KYHMEYT

References

[1]	"Nucleotide sequence, expression, and properties of luciferase coded by lux genes from a terrestrial bacterium." Szittner R., Meighen E. J. Biol. Chem. 265:16581-16587(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29999 / Hb / DSM 3368 / NCIB 12670.
[2]	"Multiple repetitive elements and organization of the lux operons of luminescent terrestrial bacteria." Meighen E.A., Szittner R.B. J. Bacteriol. 174:5371-5381(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29999 / Hb / DSM 3368 / NCIB 12670.
[3]	"The nucleotide sequence of the luxA and luxB genes of Xenorhabdus luminescens HM and a comparison of the amino acid sequences of luciferases from four species of bioluminescent bacteria." Johnston T.C., Rucker E.B., Cochrum L., Hruska K.S., Vandegrift V. Biochem. Biophys. Res. Commun. 170:407-415(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Hm.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M57416 Genomic DNA. Translation: AAA27624.1. M90093 Genomic DNA. Translation: AAA27620.1. M55977 Genomic DNA. Translation: AAA27627.1.
PIR	C35411.
3D structure databases
ProteinModelPortal	P19840.
SMR	P19840. Positions 1-319.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.30. 1 hit.
InterPro	IPR018235. Bacterial_luciferase_CS. IPR011251. Luciferase-like_dom. IPR002103. Luciferase_bac. [Graphical view]
Pfam	PF00296. Bac_luciferase. 1 hit. [Graphical view]
PRINTS	PR00089. LUCIFERASE.
SUPFAM	SSF51679. Luciferase_like. 1 hit.
PROSITE	PS00494. BACTERIAL_LUCIFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LUXB1_PHOLU

Accession

Primary (citable) accession number: P19840

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	April 3, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program