Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P19840 (LUXB1_PHOLU) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alkanal monooxygenase beta chain

EC=1.14.14.3
Alternative name(s):
Bacterial luciferase beta chain
Gene names
Name:luxB
OrganismPhotorhabdus luminescens (Xenorhabdus luminescens)
Taxonomic identifier29488 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePhotorhabdus

Protein attributes

Sequence length327 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Light-emitting reaction in luminous bacteria. The specific role of the beta subunit is unknown, but it is absolutely required for bioluminescence activity.

Catalytic activity

RCHO + reduced FMN + O2 = RCOOH + FMN + H2O + light.

Subunit structure

Heterodimer of an alpha and a beta chain.

Sequence similarities

Belongs to the bacterial luciferase oxidoreductase family.

Ontologies

Keywords
   Biological processLuminescence
   LigandFlavoprotein
FMN
   Molecular functionMonooxygenase
Oxidoreductase
Photoprotein
Gene Ontology (GO)
   Biological_processbioluminescence

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionalkanal monooxygenase (FMN-linked) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 327327Alkanal monooxygenase beta chain
PRO_0000220177

Natural variations

Natural variant851A → R in strain: Hm.

Sequences

Sequence LengthMass (Da)Tools
P19840 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 1905AF01658BE56E

FASTA32737,595
        10         20         30         40         50         60 
MKFGLFFLNF INSTTVQEQS IVRMQEITEY VDKLNFEQIL VYENHFSDNG VVGAPLTVSG 

        70         80         90        100        110        120 
FLLGLTEKIK IGSLNHIITT HHPVAIAEEA CLLDQLSEGR FILGFSDCEK KDEMHFFNRP 

       130        140        150        160        170        180 
VEYQQQLFEE CYEIINDALT TGYCNPDNDF YSFPKISVNP HAYTPGGPRK YVTATSHHIV 

       190        200        210        220        230        240 
EWAAKKGIPL IFKWDDSNDV RYEYAERYKA VADKYDVDLS EIDHQLMILV NYNEDSNKAK 

       250        260        270        280        290        300 
QETRAFISDY VLEMHPNENF ENKLEEIIAE NAVGNYTECI TAAKLAIEKC GAKSVLLSFE 

       310        320 
PMNDLMSQKN VINIVDDNIK KYHMEYT 

« Hide

References

[1]"Nucleotide sequence, expression, and properties of luciferase coded by lux genes from a terrestrial bacterium."
Szittner R., Meighen E.
J. Biol. Chem. 265:16581-16587(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 29999 / Hb / DSM 3368 / NCIB 12670.
[2]"Multiple repetitive elements and organization of the lux operons of luminescent terrestrial bacteria."
Meighen E.A., Szittner R.B.
J. Bacteriol. 174:5371-5381(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 29999 / Hb / DSM 3368 / NCIB 12670.
[3]"The nucleotide sequence of the luxA and luxB genes of Xenorhabdus luminescens HM and a comparison of the amino acid sequences of luciferases from four species of bioluminescent bacteria."
Johnston T.C., Rucker E.B., Cochrum L., Hruska K.S., Vandegrift V.
Biochem. Biophys. Res. Commun. 170:407-415(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Hm.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M57416 Genomic DNA. Translation: AAA27624.1.
M90093 Genomic DNA. Translation: AAA27620.1.
M55977 Genomic DNA. Translation: AAA27627.1.
PIRC35411.

3D structure databases

ProteinModelPortalP19840.
SMRP19840. Positions 1-319.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP19840.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.30. 1 hit.
InterProIPR018235. Bacterial_luciferase_CS.
IPR011251. Luciferase-like_dom.
IPR002103. Luciferase_bac.
[Graphical view]
PfamPF00296. Bac_luciferase. 1 hit.
[Graphical view]
PRINTSPR00089. LUCIFERASE.
SUPFAMSSF51679. SSF51679. 1 hit.
PROSITEPS00494. BACTERIAL_LUCIFERASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLUXB1_PHOLU
AccessionPrimary (citable) accession number: P19840
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: October 16, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families