Reviewed, UniProtKB/Swiss-Prot P19839 (LUXA1_PHOLU)

Names and origin

Protein names

Recommended name:
    Alkanal monooxygenase alpha chain
    EC=1.14.14.3
Alternative name(s):
    Bacterial luciferase alpha chain

Gene names

Name:

luxA

Organism

Photorhabdus luminescens (Xenorhabdus luminescens)

Taxonomic identifier

29488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Light-emitting reaction in luminous bacteria.
Catalytic activity	RCHO + reduced FMN + O2 = RCOOH + FMN + H2O + light.
Subunit structure	Heterodimer of an alpha and a beta chain.
Miscellaneous	The synthesis of this protein is regulated by a complex control mechanism that has been termed autoinduction.
Sequence similarities	Belongs to the bacterial luciferase oxidoreductase family.

Ontologies

Keywords
Biological process	Luminescence
Ligand	FMN Flavoprotein
Molecular function	Monooxygenase Oxidoreductase Photoprotein
Gene Ontology (GO)
Biological process	bioluminescence Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alkanal monooxygenase (FMN-linked) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Alkanal monooxygenase alpha chain		PRO_0000220167

Sequences

	Sequence		Length	Mass (Da)
	P19839-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 9410729A95DFB93E	FASTA	360	41,001
	10 20 30 40 50 60 MKFGNFLLTY QPPQFSQTEV MKRLVKLGRI SEECGFDTVW LLEHHFTEFG LLGNPYVAAA 70 80 90 100 110 120 YLLGATKKLN VGTAAIVLPT AHPVRQLEDV NLLDQMSKGR FRFGICRGLY NKDFRVFGTD 130 140 150 160 170 180 MNNSRALAEC WYGLIKNGMT EGYMEADNEH IKFHKVKVNP AAYSRGGAPV YVVAESASTT 190 200 210 220 230 240 EWAAQFGLPM ILSWIINTNE KKAQLELYNE VAQEYGHDIH NIDHCLSYIT SVDHDSIKAK 250 260 270 280 290 300 EICRKFLGHW YDSYVNATTI FDDSDQTRGY DFNKGQWRDF VLKGHKDTNR RIDYSYEINP 310 320 330 340 350 360 VGTPQECIDI IQKDIDATGI SNICCGFEAN GTVDEIIASM KLFQSDVMPF LKEKQRSLLY

References

[1]	"Nucleotide sequence, expression, and properties of luciferase coded by lux genes from a terrestrial bacterium." Szittner R., Meighen E. J. Biol. Chem. 265:16581-16587(1990) [PubMed: 2204626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29999 / Hb / DSM 3368 / NCIB 12670.
[2]	"Multiple repetitive elements and organization of the lux operons of luminescent terrestrial bacteria." Meighen E.A., Szittner R.B. J. Bacteriol. 174:5371-5381(1992) [PubMed: 1644764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29999 / Hb / DSM 3368 / NCIB 12670.
[3]	"The nucleotide sequence of the luxA and luxB genes of Xenorhabdus luminescens HM and a comparison of the amino acid sequences of luciferases from four species of bioluminescent bacteria." Johnston T.C., Rucker E.B., Cochrum L., Hruska K.S., Vandegrift V. Biochem. Biophys. Res. Commun. 170:407-415(1990) [PubMed: 2383248] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Hm.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M57416 Genomic DNA. Translation: AAA27623.1. M90093 Genomic DNA. Translation: AAA27619.1. M55977 Genomic DNA. Translation: AAA27626.1.
PIR	B35411.
3D structure databases
SMR	P19839. Positions 1-355.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.14.3. 257143.
Family and domain databases
InterPro	IPR018235. Bacterial_luciferase_CS. IPR011251. Luciferase-like. IPR002103. Luciferase_bac. [Graphical view]
Gene3D	G3DSA:3.20.20.30. Luciferase_like. 1 hit.
PRINTS	PR00089. LUCIFERASE.
PROSITE	PS00494. BACTERIAL_LUCIFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LUXA1_PHOLU

Accession

Primary (citable) accession number: P19839

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	February 9, 2010
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)