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P19837

- SPD1_NEPCL

UniProt

P19837 - SPD1_NEPCL

Protein

Spidroin-1

Gene
N/A
Organism
Nephila clavipes (Golden silk orbweaver)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 3 (05 Oct 2010)
      Previous versions | rss
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    Functioni

    Spiders' major ampullate silk possesses unique characteristics of strength and elasticity. Fibroin consists of pseudocrystalline regions of antiparallel beta-sheet interspersed with elastic amorphous segments.

    Keywords - Molecular functioni

    Silk protein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Spidroin-1
    Alternative name(s):
    Dragline silk fibroin 1
    OrganismiNephila clavipes (Golden silk orbweaver)
    Taxonomic identifieri6915 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeAraneomorphaeEntelegynaeAraneoideaNephilidaeNephila

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – 748›748Spidroin-1PRO_0000221446Add
    BLAST

    Proteomic databases

    ProMEXiP19837.

    Interactioni

    Subunit structurei

    Major subunit, with spidroin 2, of the dragline silk.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati1 – 25251Add
    BLAST
    Repeati26 – 38132Add
    BLAST
    Repeati39 – 66283Add
    BLAST
    Repeati67 – 96304Add
    BLAST
    Repeati97 – 130345Add
    BLAST
    Repeati131 – 158286Add
    BLAST
    Repeati159 – 191337Add
    BLAST
    Repeati192 – 204138Add
    BLAST
    Repeati205 – 235319Add
    BLAST
    Repeati236 – 2622710Add
    BLAST
    Repeati263 – 2923011Add
    BLAST
    Repeati293 – 3051312Add
    BLAST
    Repeati306 – 3332813Add
    BLAST
    Repeati334 – 3602714Add
    BLAST
    Repeati361 – 3943415Add
    BLAST
    Repeati395 – 4243016Add
    BLAST
    Repeati425 – 4583417Add
    BLAST
    Repeati459 – 4852718Add
    BLAST
    Repeati486 – 5122719Add
    BLAST
    Repeati513 – 5251320Add
    BLAST
    Repeati526 – 5553021Add
    BLAST
    Repeati556 – 5822722Add
    BLAST
    Repeati583 – 6123023Add
    BLAST
    Repeati613 – 6423024Add
    BLAST
    Repeati643 – 6551325Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 65565525 X approximate tandem repeatsAdd
    BLAST

    Domaini

    Highly repetitive protein characterized by regions of polyalanine and glycine-rich repeating units.

    Sequence similaritiesi

    Belongs to the silk fibroin family.Curated

    Keywords - Domaini

    Repeat

    Family and domain databases

    InterProiIPR021001. Spidroin_C.
    [Graphical view]
    PfamiPF11260. Spidroin_MaSp. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    P19837-1 [UniParc]FASTAAdd to Basket

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    QGAGAAAAAA GGAGQGGYGG LGGQGAGQGG YGGLGGQGAG QGAGAAAAAA    50
    AGGAGQGGYG GLGSQGAGRG GQGAGAAAAA AGGAGQGGYG GLGSQGAGRG 100
    GLGGQGAGAA AAAAAGGAGQ GGYGGLGNQG AGRGGQGAAA AAAGGAGQGG 150
    YGGLGSQGAG RGGLGGQGAG AAAAAAGGAG QGGYGGLGGQ GAGQGGYGGL 200
    GSQGAGRGGL GGQGAGAAAA AAAGGAGQGG LGGQGAGQGA GASAAAAGGA 250
    GQGGYGGLGS QGAGRGGEGA GAAAAAAGGA GQGGYGGLGG QGAGQGGYGG 300
    LGSQGAGRGG LGGQGAGAAA AGGAGQGGLG GQGAGQGAGA AAAAAGGAGQ 350
    GGYGGLGSQG AGRGGLGGQG AGAVAAAAAG GAGQGGYGGL GSQGAGRGGQ 400
    GAGAAAAAAG GAGQRGYGGL GNQGAGRGGL GGQGAGAAAA AAAGGAGQGG 450
    YGGLGNQGAG RGGQGAAAAA GGAGQGGYGG LGSQGAGRGG QGAGAAAAAA 500
    VGAGQEGIRG QGAGQGGYGG LGSQGSGRGG LGGQGAGAAA AAAGGAGQGG 550
    LGGQGAGQGA GAAAAAAGGV RQGGYGGLGS QGAGRGGQGA GAAAAAAGGA 600
    GQGGYGGLGG QGVGRGGLGG QGAGAAAAGG AGQGGYGGVG SGASAASAAA 650
    SRLSSPQASS RVSSAVSNLV ASGPTNSAAL SSTISNVVSQ IGASNPGLSG 700
    CDVLIQALLE VVSALIQILG SSSIGQVNYG SAGQATQIVG QSVYQALG 748
    Length:748
    Mass (Da):60,585
    Last modified:October 5, 2010 - v3
    Checksum:i70F50E44B0D649E0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11
    Sequence conflicti662 – 6621V → L in AAA29380. (PubMed:2402494)Curated
    Sequence conflicti672 – 6721S → T in AAA29380. (PubMed:2402494)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M37137 mRNA. Translation: AAA29380.2.
    U03848 Unassigned DNA. Translation: AAB60212.1.
    PIRiA36068.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    The tiptoe of an airbus - Issue 24 of July 2002

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M37137 mRNA. Translation: AAA29380.2 .
    U03848 Unassigned DNA. Translation: AAB60212.1 .
    PIRi A36068.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    ProMEXi P19837.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR021001. Spidroin_C.
    [Graphical view ]
    Pfami PF11260. Spidroin_MaSp. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure of a protein superfiber: spider dragline silk."
      Xu M., Lewis R.V.
      Proc. Natl. Acad. Sci. U.S.A. 87:7120-7124(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    2. Xu M., Lewis R.V.
      Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO C-TERMINUS.
    3. "Sequence conservation in the C-terminal region of spider silk proteins (Spidroin) from Nephila clavipes (Tetragnathidae) and Araneus bicentenarius (Araneidae)."
      Beckwitt R., Arcidiacono S.
      J. Biol. Chem. 269:6661-6663(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 653-747.

    Entry informationi

    Entry nameiSPD1_NEPCL
    AccessioniPrimary (citable) accession number: P19837
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: October 5, 2010
    Last modified: October 1, 2014
    This is version 65 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3