P19837 (SPD1_NEPCL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Spidroin-1 Alternative name(s): Dragline silk fibroin 1
Organism	Nephila clavipes (Golden silk orbweaver)
Taxonomic identifier	6915 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Araneae › Araneomorphae › Entelegynae › Orbiculariae › Araneoidea › Nephilidae › Nephila

Protein attributes

Sequence length	748 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Spiders' major ampullate silk possesses unique characteristics of strength and elasticity. Fibroin consists of pseudocrystalline regions of antiparallel beta-sheet interspersed with elastic amorphous segments.
Subunit structure	Major subunit, with spidroin 2, of the dragline silk.
Subcellular location	Secreted › extracellular space.
Domain	Highly repetitive protein characterized by regions of polyalanine and glycine-rich repeating units.
Sequence similarities	Belongs to the silk fibroin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Repeat
Molecular function	Silk protein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 748	›748	Spidroin-1		PRO_0000221446
Regions
	Repeat	1 – 25	25	1
	Repeat	26 – 38	13	2
	Repeat	39 – 66	28	3
	Repeat	67 – 96	30	4
	Repeat	97 – 130	34	5
	Repeat	131 – 158	28	6
	Repeat	159 – 191	33	7
	Repeat	192 – 204	13	8
	Repeat	205 – 235	31	9
	Repeat	236 – 262	27	10
	Repeat	263 – 292	30	11
	Repeat	293 – 305	13	12
	Repeat	306 – 333	28	13
	Repeat	334 – 360	27	14
	Repeat	361 – 394	34	15
	Repeat	395 – 424	30	16
	Repeat	425 – 458	34	17
	Repeat	459 – 485	27	18
	Repeat	486 – 512	27	19
	Repeat	513 – 525	13	20
	Repeat	526 – 555	30	21
	Repeat	556 – 582	27	22
	Repeat	583 – 612	30	23
	Repeat	613 – 642	30	24
	Repeat	643 – 655	13	25
	Region	1 – 655	655	25 X approximate tandem repeats
Experimental info
	Sequence conflict	662	1	V → L in AAA29380. Ref.1
	Sequence conflict	672	1	S → T in AAA29380. Ref.1
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P19837 [UniParc]. Last modified October 5, 2010. Version 3. Checksum: 70F50E44B0D649E0	FASTA	748	60,585
	10 20 30 40 50 60 QGAGAAAAAA GGAGQGGYGG LGGQGAGQGG YGGLGGQGAG QGAGAAAAAA AGGAGQGGYG 70 80 90 100 110 120 GLGSQGAGRG GQGAGAAAAA AGGAGQGGYG GLGSQGAGRG GLGGQGAGAA AAAAAGGAGQ 130 140 150 160 170 180 GGYGGLGNQG AGRGGQGAAA AAAGGAGQGG YGGLGSQGAG RGGLGGQGAG AAAAAAGGAG 190 200 210 220 230 240 QGGYGGLGGQ GAGQGGYGGL GSQGAGRGGL GGQGAGAAAA AAAGGAGQGG LGGQGAGQGA 250 260 270 280 290 300 GASAAAAGGA GQGGYGGLGS QGAGRGGEGA GAAAAAAGGA GQGGYGGLGG QGAGQGGYGG 310 320 330 340 350 360 LGSQGAGRGG LGGQGAGAAA AGGAGQGGLG GQGAGQGAGA AAAAAGGAGQ GGYGGLGSQG 370 380 390 400 410 420 AGRGGLGGQG AGAVAAAAAG GAGQGGYGGL GSQGAGRGGQ GAGAAAAAAG GAGQRGYGGL 430 440 450 460 470 480 GNQGAGRGGL GGQGAGAAAA AAAGGAGQGG YGGLGNQGAG RGGQGAAAAA GGAGQGGYGG 490 500 510 520 530 540 LGSQGAGRGG QGAGAAAAAA VGAGQEGIRG QGAGQGGYGG LGSQGSGRGG LGGQGAGAAA 550 560 570 580 590 600 AAAGGAGQGG LGGQGAGQGA GAAAAAAGGV RQGGYGGLGS QGAGRGGQGA GAAAAAAGGA 610 620 630 640 650 660 GQGGYGGLGG QGVGRGGLGG QGAGAAAAGG AGQGGYGGVG SGASAASAAA SRLSSPQASS 670 680 690 700 710 720 RVSSAVSNLV ASGPTNSAAL SSTISNVVSQ IGASNPGLSG CDVLIQALLE VVSALIQILG 730 740 SSSIGQVNYG SAGQATQIVG QSVYQALG

References

[1]	"Structure of a protein superfiber: spider dragline silk." Xu M., Lewis R.V. Proc. Natl. Acad. Sci. U.S.A. 87:7120-7124(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	Xu M., Lewis R.V. Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO C-TERMINUS.
[3]	"Sequence conservation in the C-terminal region of spider silk proteins (Spidroin) from Nephila clavipes (Tetragnathidae) and Araneus bicentenarius (Araneidae)." Beckwitt R., Arcidiacono S. J. Biol. Chem. 269:6661-6663(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 653-747.

Web resources

Protein Spotlight

The tiptoe of an airbus - Issue 24 of July 2002

Cross-references

Sequence databases
EMBL GenBank DDBJ	M37137 mRNA. Translation: AAA29380.2. U03848 Unassigned DNA. Translation: AAB60212.1.
PIR	A36068.
3D structure databases
ModBase	Search...
Proteomic databases
ProMEX	P19837.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR021001. Spidroin_C. [Graphical view]
Pfam	PF11260. Spidroin_MaSp. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPD1_NEPCL

Accession

Primary (citable) accession number: P19837

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	October 5, 2010
Last modified:	April 3, 2013
This is version 63 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)