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Protein

Spidroin-1

Gene
N/A
Organism
Nephila clavipes (Golden silk orbweaver)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Spiders' major ampullate silk possesses unique characteristics of strength and elasticity. Fibroin consists of pseudocrystalline regions of antiparallel beta-sheet interspersed with elastic amorphous segments.

Keywords - Molecular functioni

Silk protein

Names & Taxonomyi

Protein namesi
Recommended name:
Spidroin-1
Alternative name(s):
Dragline silk fibroin 1
OrganismiNephila clavipes (Golden silk orbweaver)
Taxonomic identifieri6915 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeAraneomorphaeEntelegynaeAraneoideaNephilidaeNephila

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000221446‹1 – 748Spidroin-1Add BLAST›748

Interactioni

Subunit structurei

Major subunit, with spidroin 2, of the dragline silk.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati1 – 251Add BLAST25
Repeati26 – 382Add BLAST13
Repeati39 – 663Add BLAST28
Repeati67 – 964Add BLAST30
Repeati97 – 1305Add BLAST34
Repeati131 – 1586Add BLAST28
Repeati159 – 1917Add BLAST33
Repeati192 – 2048Add BLAST13
Repeati205 – 2359Add BLAST31
Repeati236 – 26210Add BLAST27
Repeati263 – 29211Add BLAST30
Repeati293 – 30512Add BLAST13
Repeati306 – 33313Add BLAST28
Repeati334 – 36014Add BLAST27
Repeati361 – 39415Add BLAST34
Repeati395 – 42416Add BLAST30
Repeati425 – 45817Add BLAST34
Repeati459 – 48518Add BLAST27
Repeati486 – 51219Add BLAST27
Repeati513 – 52520Add BLAST13
Repeati526 – 55521Add BLAST30
Repeati556 – 58222Add BLAST27
Repeati583 – 61223Add BLAST30
Repeati613 – 64224Add BLAST30
Repeati643 – 65525Add BLAST13

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 65525 X approximate tandem repeatsAdd BLAST655

Domaini

Highly repetitive protein characterized by regions of polyalanine and glycine-rich repeating units.

Sequence similaritiesi

Belongs to the silk fibroin family.Curated

Keywords - Domaini

Repeat

Family and domain databases

InterProiIPR021001. Spidroin_C.
[Graphical view]
PfamiPF11260. Spidroin_MaSp. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

P19837-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
QGAGAAAAAA GGAGQGGYGG LGGQGAGQGG YGGLGGQGAG QGAGAAAAAA
60 70 80 90 100
AGGAGQGGYG GLGSQGAGRG GQGAGAAAAA AGGAGQGGYG GLGSQGAGRG
110 120 130 140 150
GLGGQGAGAA AAAAAGGAGQ GGYGGLGNQG AGRGGQGAAA AAAGGAGQGG
160 170 180 190 200
YGGLGSQGAG RGGLGGQGAG AAAAAAGGAG QGGYGGLGGQ GAGQGGYGGL
210 220 230 240 250
GSQGAGRGGL GGQGAGAAAA AAAGGAGQGG LGGQGAGQGA GASAAAAGGA
260 270 280 290 300
GQGGYGGLGS QGAGRGGEGA GAAAAAAGGA GQGGYGGLGG QGAGQGGYGG
310 320 330 340 350
LGSQGAGRGG LGGQGAGAAA AGGAGQGGLG GQGAGQGAGA AAAAAGGAGQ
360 370 380 390 400
GGYGGLGSQG AGRGGLGGQG AGAVAAAAAG GAGQGGYGGL GSQGAGRGGQ
410 420 430 440 450
GAGAAAAAAG GAGQRGYGGL GNQGAGRGGL GGQGAGAAAA AAAGGAGQGG
460 470 480 490 500
YGGLGNQGAG RGGQGAAAAA GGAGQGGYGG LGSQGAGRGG QGAGAAAAAA
510 520 530 540 550
VGAGQEGIRG QGAGQGGYGG LGSQGSGRGG LGGQGAGAAA AAAGGAGQGG
560 570 580 590 600
LGGQGAGQGA GAAAAAAGGV RQGGYGGLGS QGAGRGGQGA GAAAAAAGGA
610 620 630 640 650
GQGGYGGLGG QGVGRGGLGG QGAGAAAAGG AGQGGYGGVG SGASAASAAA
660 670 680 690 700
SRLSSPQASS RVSSAVSNLV ASGPTNSAAL SSTISNVVSQ IGASNPGLSG
710 720 730 740
CDVLIQALLE VVSALIQILG SSSIGQVNYG SAGQATQIVG QSVYQALG
Length:748
Mass (Da):60,585
Last modified:October 5, 2010 - v3
Checksum:i70F50E44B0D649E0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11
Sequence conflicti662V → L in AAA29380 (PubMed:2402494).Curated1
Sequence conflicti672S → T in AAA29380 (PubMed:2402494).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37137 mRNA. Translation: AAA29380.2.
U03848 Unassigned DNA. Translation: AAB60212.1.
PIRiA36068.

Cross-referencesi

Web resourcesi

Protein Spotlight

The tiptoe of an airbus - Issue 24 of July 2002

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37137 mRNA. Translation: AAA29380.2.
U03848 Unassigned DNA. Translation: AAB60212.1.
PIRiA36068.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR021001. Spidroin_C.
[Graphical view]
PfamiPF11260. Spidroin_MaSp. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPD1_NEPCL
AccessioniPrimary (citable) accession number: P19837
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: October 5, 2010
Last modified: January 7, 2015
This is version 68 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.