Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P19837 (SPD1_NEPCL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Spidroin-1
Alternative name(s):
Dragline silk fibroin 1
OrganismNephila clavipes (Golden silk orbweaver)
Taxonomic identifier6915 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeAraneomorphaeEntelegynaeAraneoideaNephilidaeNephila

Protein attributes

Sequence length748 AA.
Sequence statusFragment.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Spiders' major ampullate silk possesses unique characteristics of strength and elasticity. Fibroin consists of pseudocrystalline regions of antiparallel beta-sheet interspersed with elastic amorphous segments.

Subunit structure

Major subunit, with spidroin 2, of the dragline silk.

Subcellular location

Secretedextracellular space.

Domain

Highly repetitive protein characterized by regions of polyalanine and glycine-rich repeating units.

Sequence similarities

Belongs to the silk fibroin family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainRepeat
   Molecular functionSilk protein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Cellular_componentextracellular space

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 748›748Spidroin-1
PRO_0000221446

Regions

Repeat1 – 25251
Repeat26 – 38132
Repeat39 – 66283
Repeat67 – 96304
Repeat97 – 130345
Repeat131 – 158286
Repeat159 – 191337
Repeat192 – 204138
Repeat205 – 235319
Repeat236 – 2622710
Repeat263 – 2923011
Repeat293 – 3051312
Repeat306 – 3332813
Repeat334 – 3602714
Repeat361 – 3943415
Repeat395 – 4243016
Repeat425 – 4583417
Repeat459 – 4852718
Repeat486 – 5122719
Repeat513 – 5251320
Repeat526 – 5553021
Repeat556 – 5822722
Repeat583 – 6123023
Repeat613 – 6423024
Repeat643 – 6551325
Region1 – 65565525 X approximate tandem repeats

Experimental info

Sequence conflict6621V → L in AAA29380. Ref.1
Sequence conflict6721S → T in AAA29380. Ref.1
Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
P19837 [UniParc].

Last modified October 5, 2010. Version 3.
Checksum: 70F50E44B0D649E0

FASTA74860,585
        10         20         30         40         50         60 
QGAGAAAAAA GGAGQGGYGG LGGQGAGQGG YGGLGGQGAG QGAGAAAAAA AGGAGQGGYG 

        70         80         90        100        110        120 
GLGSQGAGRG GQGAGAAAAA AGGAGQGGYG GLGSQGAGRG GLGGQGAGAA AAAAAGGAGQ 

       130        140        150        160        170        180 
GGYGGLGNQG AGRGGQGAAA AAAGGAGQGG YGGLGSQGAG RGGLGGQGAG AAAAAAGGAG 

       190        200        210        220        230        240 
QGGYGGLGGQ GAGQGGYGGL GSQGAGRGGL GGQGAGAAAA AAAGGAGQGG LGGQGAGQGA 

       250        260        270        280        290        300 
GASAAAAGGA GQGGYGGLGS QGAGRGGEGA GAAAAAAGGA GQGGYGGLGG QGAGQGGYGG 

       310        320        330        340        350        360 
LGSQGAGRGG LGGQGAGAAA AGGAGQGGLG GQGAGQGAGA AAAAAGGAGQ GGYGGLGSQG 

       370        380        390        400        410        420 
AGRGGLGGQG AGAVAAAAAG GAGQGGYGGL GSQGAGRGGQ GAGAAAAAAG GAGQRGYGGL 

       430        440        450        460        470        480 
GNQGAGRGGL GGQGAGAAAA AAAGGAGQGG YGGLGNQGAG RGGQGAAAAA GGAGQGGYGG 

       490        500        510        520        530        540 
LGSQGAGRGG QGAGAAAAAA VGAGQEGIRG QGAGQGGYGG LGSQGSGRGG LGGQGAGAAA 

       550        560        570        580        590        600 
AAAGGAGQGG LGGQGAGQGA GAAAAAAGGV RQGGYGGLGS QGAGRGGQGA GAAAAAAGGA 

       610        620        630        640        650        660 
GQGGYGGLGG QGVGRGGLGG QGAGAAAAGG AGQGGYGGVG SGASAASAAA SRLSSPQASS 

       670        680        690        700        710        720 
RVSSAVSNLV ASGPTNSAAL SSTISNVVSQ IGASNPGLSG CDVLIQALLE VVSALIQILG 

       730        740 
SSSIGQVNYG SAGQATQIVG QSVYQALG 

« Hide

References

[1]"Structure of a protein superfiber: spider dragline silk."
Xu M., Lewis R.V.
Proc. Natl. Acad. Sci. U.S.A. 87:7120-7124(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]Xu M., Lewis R.V.
Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO C-TERMINUS.
[3]"Sequence conservation in the C-terminal region of spider silk proteins (Spidroin) from Nephila clavipes (Tetragnathidae) and Araneus bicentenarius (Araneidae)."
Beckwitt R., Arcidiacono S.
J. Biol. Chem. 269:6661-6663(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 653-747.

Web resources

Protein Spotlight

The tiptoe of an airbus - Issue 24 of July 2002

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M37137 mRNA. Translation: AAA29380.2.
U03848 Unassigned DNA. Translation: AAB60212.1.
PIRA36068.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Proteomic databases

ProMEXP19837.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR021001. Spidroin_C.
[Graphical view]
PfamPF11260. Spidroin_MaSp. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSPD1_NEPCL
AccessionPrimary (citable) accession number: P19837
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: October 5, 2010
Last modified: February 19, 2014
This is version 64 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries