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Protein

DNA polymerase I, thermostable

Gene

polA

Organism
Thermus aquaticus
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BRENDAi2.7.7.7. 6334.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase I, thermostable (EC:2.7.7.7)
Alternative name(s):
Taq polymerase 1
Gene namesi
Name:polA
Synonyms:pol1
OrganismiThermus aquaticus
Taxonomic identifieri271 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Pathology & Biotechi

Biotechnological usei

Used in the PCR method because of its high thermostability. Has a relatively high error rate because it lacks exonuclease proofreading functionality.

Chemistry databases

ChEMBLiCHEMBL3564.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001012561 – 832DNA polymerase I, thermostableAdd BLAST832

Interactioni

Protein-protein interaction databases

STRINGi498848.TaqDRAFT_3881.

Chemistry databases

BindingDBiP19821.

Structurei

Secondary structure

1832
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi12 – 17Combined sources6
Helixi22 – 25Combined sources4
Helixi26 – 28Combined sources3
Beta strandi36 – 38Combined sources3
Beta strandi42 – 44Combined sources3
Helixi47 – 53Combined sources7
Helixi54 – 56Combined sources3
Beta strandi60 – 65Combined sources6
Beta strandi69 – 71Combined sources3
Beta strandi73 – 75Combined sources3
Helixi76 – 79Combined sources4
Turni80 – 82Combined sources3
Helixi95 – 97Combined sources3
Helixi99 – 105Combined sources7
Beta strandi109 – 111Combined sources3
Beta strandi114 – 116Combined sources3
Helixi118 – 132Combined sources15
Beta strandi134 – 139Combined sources6
Helixi143 – 148Combined sources6
Beta strandi150 – 156Combined sources7
Beta strandi158 – 160Combined sources3
Helixi168 – 171Combined sources4
Helixi176 – 178Combined sources3
Turni179 – 184Combined sources6
Beta strandi189 – 191Combined sources3
Beta strandi199 – 201Combined sources3
Turni203 – 207Combined sources5
Helixi208 – 210Combined sources3
Beta strandi212 – 214Combined sources3
Beta strandi216 – 220Combined sources5
Turni226 – 228Combined sources3
Helixi230 – 233Combined sources4
Beta strandi236 – 239Combined sources4
Helixi242 – 244Combined sources3
Helixi266 – 273Combined sources8
Turni274 – 277Combined sources4
Helixi279 – 288Combined sources10
Beta strandi295 – 297Combined sources3
Beta strandi298 – 300Combined sources3
Beta strandi306 – 314Combined sources9
Turni316 – 318Combined sources3
Beta strandi321 – 328Combined sources8
Beta strandi331 – 334Combined sources4
Helixi338 – 342Combined sources5
Beta strandi346 – 348Combined sources3
Helixi353 – 362Combined sources10
Helixi373 – 380Combined sources8
Helixi387 – 394Combined sources8
Helixi402 – 419Combined sources18
Turni420 – 422Combined sources3
Helixi424 – 432Combined sources9
Helixi434 – 447Combined sources14
Beta strandi449 – 451Combined sources3
Helixi453 – 478Combined sources26
Helixi487 – 495Combined sources9
Turni507 – 509Combined sources3
Helixi513 – 515Combined sources3
Helixi516 – 520Combined sources5
Turni521 – 525Combined sources5
Helixi528 – 544Combined sources17
Turni545 – 548Combined sources4
Helixi549 – 552Combined sources4
Turni555 – 557Combined sources3
Beta strandi558 – 560Combined sources3
Beta strandi563 – 567Combined sources5
Beta strandi570 – 572Combined sources3
Beta strandi575 – 579Combined sources5
Helixi581 – 583Combined sources3
Beta strandi586 – 588Combined sources3
Helixi589 – 595Combined sources7
Beta strandi604 – 611Combined sources8
Helixi614 – 623Combined sources10
Helixi626 – 633Combined sources8
Helixi638 – 647Combined sources10
Helixi651 – 653Combined sources3
Helixi656 – 670Combined sources15
Helixi675 – 680Combined sources6
Turni681 – 683Combined sources3
Helixi686 – 699Combined sources14
Helixi701 – 717Combined sources17
Beta strandi718 – 721Combined sources4
Beta strandi723 – 725Combined sources3
Beta strandi727 – 729Combined sources3
Helixi731 – 734Combined sources4
Helixi738 – 775Combined sources38
Beta strandi778 – 782Combined sources5
Beta strandi784 – 792Combined sources9
Helixi793 – 795Combined sources3
Helixi796 – 809Combined sources14
Beta strandi819 – 826Combined sources8
Helixi829 – 831Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BGXX-ray2.30T1-832[»]
1JXEX-ray2.85A293-832[»]
1KTQX-ray2.50A290-832[»]
1QSSX-ray2.30A293-831[»]
1QSYX-ray2.30A293-831[»]
1QTMX-ray2.30A293-831[»]
1TAQX-ray2.40A1-832[»]
1TAUX-ray3.00A1-832[»]
2KTQX-ray2.30A295-832[»]
3KTQX-ray2.30A293-832[»]
3LWLX-ray2.25A293-832[»]
3LWMX-ray2.19A293-832[»]
3M8RX-ray2.00A293-832[»]
3M8SX-ray2.20A293-832[»]
3OJSX-ray1.90A293-832[»]
3OJUX-ray2.00A293-832[»]
3PO4X-ray1.80A293-832[»]
3PO5X-ray2.39A293-832[»]
3PY8X-ray1.74A293-832[»]
3RR7X-ray1.95A293-832[»]
3RR8X-ray2.40A293-832[»]
3RRGX-ray2.30A293-832[»]
3RRHX-ray1.80A293-832[»]
3RTVX-ray1.90A293-832[»]
3SV3X-ray2.10A293-832[»]
3SV4X-ray1.99A293-832[»]
3SYZX-ray1.95A293-832[»]
3SZ2X-ray2.15A293-832[»]
3T3FX-ray1.90A293-832[»]
4BWJX-ray1.55A293-832[»]
4BWMX-ray1.75A293-832[»]
4C8KX-ray2.17A293-832[»]
4C8LX-ray1.70A293-832[»]
4C8MX-ray1.57A293-832[»]
4C8NX-ray1.88A293-832[»]
4C8OX-ray1.75A293-832[»]
4CCHX-ray2.55A293-832[»]
4DF4X-ray2.20A293-832[»]
4DF8X-ray2.00A293-832[»]
4DFJX-ray1.90A293-832[»]
4DFKX-ray1.65A293-832[»]
4DFMX-ray1.89A293-832[»]
4DFPX-ray2.00A293-832[»]
4DLEX-ray2.44A293-832[»]
4DLGX-ray1.89A293-832[»]
4ELTX-ray2.20A293-832[»]
4ELUX-ray1.80A293-832[»]
4ELVX-ray1.90A293-832[»]
4KTQX-ray2.50A294-832[»]
4N56X-ray2.20A281-832[»]
4N5SX-ray1.67A281-832[»]
4XIUX-ray2.50A294-832[»]
5KTQX-ray2.50A290-832[»]
ProteinModelPortaliP19821.
SMRiP19821.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP19821.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni410 – 832PolymeraseBy similarityAdd BLAST423

Sequence similaritiesi

Belongs to the DNA polymerase type-A family.Curated
Contains 1 5'-3' exonuclease domain.Curated

Phylogenomic databases

eggNOGiENOG4105C2M. Bacteria.
COG0258. LUCA.
COG0749. LUCA.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.40.50.1010. 1 hit.
InterProiIPR020046. 5-3_exonucl_a-hlix_arch_N.
IPR020045. 5-3_exonuclease_C.
IPR002421. 5-3_exonuclease_N.
IPR019760. DNA-dir_DNA_pol_A_CS.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR018320. DNA_polymerase_1.
IPR002298. DNA_polymerase_A.
IPR008918. HhH2.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR029060. PIN_domain-like.
IPR012337. RNaseH-like_dom.
IPR015361. Taq_pol_thermo_exonuc.
[Graphical view]
PfamiPF01367. 5_3_exonuc. 1 hit.
PF02739. 5_3_exonuc_N. 1 hit.
PF00476. DNA_pol_A. 1 hit.
PF09281. Taq-exonuc. 1 hit.
[Graphical view]
PRINTSiPR00868. DNAPOLI.
SMARTiSM00475. 53EXOc. 1 hit.
SM00278. HhH1. 2 hits.
SM00279. HhH2. 1 hit.
SM00482. POLAc. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF53098. SSF53098. 1 hit.
SSF88723. SSF88723. 1 hit.
TIGRFAMsiTIGR00593. pola. 1 hit.
PROSITEiPS00447. DNA_POLYMERASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19821-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGMLPLFEP KGRVLLVDGH HLAYRTFHAL KGLTTSRGEP VQAVYGFAKS
60 70 80 90 100
LLKALKEDGD AVIVVFDAKA PSFRHEAYGG YKAGRAPTPE DFPRQLALIK
110 120 130 140 150
ELVDLLGLAR LEVPGYEADD VLASLAKKAE KEGYEVRILT ADKDLYQLLS
160 170 180 190 200
DRIHVLHPEG YLITPAWLWE KYGLRPDQWA DYRALTGDES DNLPGVKGIG
210 220 230 240 250
EKTARKLLEE WGSLEALLKN LDRLKPAIRE KILAHMDDLK LSWDLAKVRT
260 270 280 290 300
DLPLEVDFAK RREPDRERLR AFLERLEFGS LLHEFGLLES PKALEEAPWP
310 320 330 340 350
PPEGAFVGFV LSRKEPMWAD LLALAAARGG RVHRAPEPYK ALRDLKEARG
360 370 380 390 400
LLAKDLSVLA LREGLGLPPG DDPMLLAYLL DPSNTTPEGV ARRYGGEWTE
410 420 430 440 450
EAGERAALSE RLFANLWGRL EGEERLLWLY REVERPLSAV LAHMEATGVR
460 470 480 490 500
LDVAYLRALS LEVAEEIARL EAEVFRLAGH PFNLNSRDQL ERVLFDELGL
510 520 530 540 550
PAIGKTEKTG KRSTSAAVLE ALREAHPIVE KILQYRELTK LKSTYIDPLP
560 570 580 590 600
DLIHPRTGRL HTRFNQTATA TGRLSSSDPN LQNIPVRTPL GQRIRRAFIA
610 620 630 640 650
EEGWLLVALD YSQIELRVLA HLSGDENLIR VFQEGRDIHT ETASWMFGVP
660 670 680 690 700
REAVDPLMRR AAKTINFGVL YGMSAHRLSQ ELAIPYEEAQ AFIERYFQSF
710 720 730 740 750
PKVRAWIEKT LEEGRRRGYV ETLFGRRRYV PDLEARVKSV REAAERMAFN
760 770 780 790 800
MPVQGTAADL MKLAMVKLFP RLEEMGARML LQVHDELVLE APKERAEAVA
810 820 830
RLAKEVMEGV YPLAVPLEVE VGIGEDWLSA KE
Length:832
Mass (Da):93,910
Last modified:February 1, 1991 - v1
Checksum:iF1731055B5246F03
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti155V → A in BAA06775 (PubMed:7896728).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04639 Genomic DNA. Translation: AAA27507.1.
D32013 Genomic DNA. Translation: BAA06775.1.
PIRiA33530.
JX0359.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04639 Genomic DNA. Translation: AAA27507.1.
D32013 Genomic DNA. Translation: BAA06775.1.
PIRiA33530.
JX0359.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BGXX-ray2.30T1-832[»]
1JXEX-ray2.85A293-832[»]
1KTQX-ray2.50A290-832[»]
1QSSX-ray2.30A293-831[»]
1QSYX-ray2.30A293-831[»]
1QTMX-ray2.30A293-831[»]
1TAQX-ray2.40A1-832[»]
1TAUX-ray3.00A1-832[»]
2KTQX-ray2.30A295-832[»]
3KTQX-ray2.30A293-832[»]
3LWLX-ray2.25A293-832[»]
3LWMX-ray2.19A293-832[»]
3M8RX-ray2.00A293-832[»]
3M8SX-ray2.20A293-832[»]
3OJSX-ray1.90A293-832[»]
3OJUX-ray2.00A293-832[»]
3PO4X-ray1.80A293-832[»]
3PO5X-ray2.39A293-832[»]
3PY8X-ray1.74A293-832[»]
3RR7X-ray1.95A293-832[»]
3RR8X-ray2.40A293-832[»]
3RRGX-ray2.30A293-832[»]
3RRHX-ray1.80A293-832[»]
3RTVX-ray1.90A293-832[»]
3SV3X-ray2.10A293-832[»]
3SV4X-ray1.99A293-832[»]
3SYZX-ray1.95A293-832[»]
3SZ2X-ray2.15A293-832[»]
3T3FX-ray1.90A293-832[»]
4BWJX-ray1.55A293-832[»]
4BWMX-ray1.75A293-832[»]
4C8KX-ray2.17A293-832[»]
4C8LX-ray1.70A293-832[»]
4C8MX-ray1.57A293-832[»]
4C8NX-ray1.88A293-832[»]
4C8OX-ray1.75A293-832[»]
4CCHX-ray2.55A293-832[»]
4DF4X-ray2.20A293-832[»]
4DF8X-ray2.00A293-832[»]
4DFJX-ray1.90A293-832[»]
4DFKX-ray1.65A293-832[»]
4DFMX-ray1.89A293-832[»]
4DFPX-ray2.00A293-832[»]
4DLEX-ray2.44A293-832[»]
4DLGX-ray1.89A293-832[»]
4ELTX-ray2.20A293-832[»]
4ELUX-ray1.80A293-832[»]
4ELVX-ray1.90A293-832[»]
4KTQX-ray2.50A294-832[»]
4N56X-ray2.20A281-832[»]
4N5SX-ray1.67A281-832[»]
4XIUX-ray2.50A294-832[»]
5KTQX-ray2.50A290-832[»]
ProteinModelPortaliP19821.
SMRiP19821.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi498848.TaqDRAFT_3881.

Chemistry databases

BindingDBiP19821.
ChEMBLiCHEMBL3564.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105C2M. Bacteria.
COG0258. LUCA.
COG0749. LUCA.

Enzyme and pathway databases

BRENDAi2.7.7.7. 6334.

Miscellaneous databases

EvolutionaryTraceiP19821.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.40.50.1010. 1 hit.
InterProiIPR020046. 5-3_exonucl_a-hlix_arch_N.
IPR020045. 5-3_exonuclease_C.
IPR002421. 5-3_exonuclease_N.
IPR019760. DNA-dir_DNA_pol_A_CS.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR018320. DNA_polymerase_1.
IPR002298. DNA_polymerase_A.
IPR008918. HhH2.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR029060. PIN_domain-like.
IPR012337. RNaseH-like_dom.
IPR015361. Taq_pol_thermo_exonuc.
[Graphical view]
PfamiPF01367. 5_3_exonuc. 1 hit.
PF02739. 5_3_exonuc_N. 1 hit.
PF00476. DNA_pol_A. 1 hit.
PF09281. Taq-exonuc. 1 hit.
[Graphical view]
PRINTSiPR00868. DNAPOLI.
SMARTiSM00475. 53EXOc. 1 hit.
SM00278. HhH1. 2 hits.
SM00279. HhH2. 1 hit.
SM00482. POLAc. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF53098. SSF53098. 1 hit.
SSF88723. SSF88723. 1 hit.
TIGRFAMsiTIGR00593. pola. 1 hit.
PROSITEiPS00447. DNA_POLYMERASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPO1_THEAQ
AccessioniPrimary (citable) accession number: P19821
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 2, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.