Reviewed, UniProtKB/Swiss-Prot P19817 (RFAJ_SALTY)

Names and origin

Protein names

Recommended name:
    Lipopolysaccharide 1,2-glucosyltransferase
    EC=2.4.1.58

Gene names

Name:	rfaJ
Synonyms:	waaJ
Ordered Locus Names:	STM3717

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Adds the glucose(II) group on the galactose(I) group of LPS.
Catalytic activity	UDP-glucose + lipopolysaccharide = UDP + D-glucosyl-lipopolysaccharide.
Pathway	Bacterial outer membrane biogenesis; LPS core biosynthesis.
Sequence similarities	Belongs to the glycosyltransferase 8 family.
Sequence caution	The sequence CAA37842.1 differs from that shown. Reason: Frameshift at positions 42, 138, 235 and 264.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	lipopolysaccharide 3-alpha-galactosyltransferase activity Inferred from electronic annotation. Source: InterPro lipopolysaccharide glucosyltransferase I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Lipopolysaccharide 1,2-glucosyltransferase		PRO_0000206067

Sequences

	Sequence		Length	Mass (Da)
	P19817-1 [UniParc]. Last modified December 15, 1998. Version 4. Checksum: AAB23E412429E00B	FASTA	336	38,763
	10 20 30 40 50 60 MDSFPEIEIA EYKVFDESNN NDDNVLNISY GVDENYLDGV GVSIASVVLN NNIPLAFHII 70 80 90 100 110 120 CDSYSPCFVK YIERLAVQHH IKISLYLIKV ESLEVLPQTK VWSRAMYFRL FAFDYLSKKV 130 140 150 160 170 180 NTLLYLDADV VCKGSLQDLL QLDLTEKIAA VVKDVDSIQN KVNERLSAFN LQGGYFNSGV 190 200 210 220 230 240 VFVNLKLWKE NALTKKAFLL LAGKEADSFK YPDQDVLNIL LQDKVIFLPR PYNTIYTIKS 250 260 270 280 290 300 ELKDKSHKKY SNIINDNTIL IHYTGATKPW HAWANYPSVI YYKNARLNSP WKDFPAKDAR 310 320 330 TIVEFKKRYK HLLVQGHYFK GLLAGSAYLY RKLFHK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of rfaI and rfaJ genes encoding lipopolysaccharide glycosyl transferases from Salmonella typhimurium." Carstenius P., Flock J.-I., Lindberg A. Nucleic Acids Res. 18:6128-6128(1990) [PubMed: 2235496] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"The assembly system for the lipopolysaccharide R2 core-type of Escherichia coli is a hybrid of those found in Escherichia coli K-12 and Salmonella enterica. Structure and function of the R2 WaaK and WaaL homologs." Heinrichs D.E., Monteiro M.A., Perry M.B., Whitfield C. J. Biol. Chem. 273:8849-8859(1998) [PubMed: 9535865] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[3]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X53847 Genomic DNA. Translation: CAA37842.1. Frameshift. AF026386 Genomic DNA. Translation: AAC16411.1. AE006468 Genomic DNA. Translation: AAL22576.1.
PIR	S12098.
RefSeq	NP_462617.1.
3D structure databases
SMR	P19817. Positions 26-279.
ModBase	Search...
Protein family/group databases
CAZy	GT8. Glycosyltransferase Family 8.
Proteomic databases
PRIDE	P19817.
Genome annotation databases
GeneID	1255241.
GenomeReviews	Gene locus STM3717 in contig AE006468_GR.
KEGG	stm:STM3717.
NMPDR	fig|99287.1.peg.3593.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG417202.
OMA	NVPELNV.
Enzyme and pathway databases
BioCyc	STYP99287:STM3717-MONOMER.
BRENDA	2.4.1.58. 2.
Family and domain databases
InterPro	IPR002495. Glyco_trans_8. IPR013645. Glyco_transf_8N. [Graphical view]
Pfam	PF01501. Glyco_transf_8. 1 hit. PF08437. Glyco_transf_8N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RFAJ_SALTY

Accession

Primary (citable) accession number: P19817
Secondary accession number(s): O68266

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	December 15, 1998
Last modified:	January 19, 2010
This is version 75 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)