Reviewed, UniProtKB/Swiss-Prot P19816 (RFAI_SALTY)

Names and originHide

Protein names

Recommended name:
Lipopolysaccharide 1,3-galactosyltransferase
EC=2.4.1.44

Alternative name(s):
Lipopolysaccharide 3-alpha-galactosyltransferase

Gene names

Name:	rfaI
Synonyms:	waaI
Ordered Locus Names:	STM3718

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributesHide

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)Hide

Function	Adds the galactose(I) group on the glucose(I) group of LPS.
Catalytic activity	UDP-galactose + lipopolysaccharide = UDP + 3-alpha-D-galactosyl-[lipopolysaccharide glucose].
Pathway	Bacterial outer membrane biogenesis; LPS core biosynthesis.
Sequence similarities	Belongs to the glycosyltransferase 8 family.
Sequence caution	The sequence CAA37841.1 differs from that shown. Reason: Frameshift at positions 117, 125, 140 and 166.

OntologiesHide

Keywords
Biological process	Lipopolysaccharide biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	lipopolysaccharide 3-alpha-galactosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)Hide

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Lipopolysaccharide 1,3-galactosyltransferase		PRO_0000206065
Experimental info
	Sequence conflict	28 – 33	6	FNIAYG → LYSLW in CAA37841. Ref.1
	Sequence conflict	59	1	A → R in CAA37841. Ref.1
	Sequence conflict	210	1	E → Q in AAC16412. Ref.2

SequencesHide

	Sequence		Length	Mass (Da)
	P19816-1 [UniParc]. Last modified January 23, 2002. Version 4. Checksum: 6DB1FA1531AE25F7	FASTA	337	38,905
	10 20 30 40 50 60 MSRKYFEEEV IQQTLDYNYA QHSDADKFNI AYGIDKNFLF GCGVSIASVL LANPEKALAF 70 80 90 100 110 120 HVFTDFFDSE DQQRFEALAK QYATQIVVYL IDCERLKSLP STKNWTYATY FRFIIADYFS 130 140 150 160 170 180 DKTDRVLYLD ADIACKGSIQ ELIDLNFAEN EIAAVVAEGE LEWWTKRSVS LATPGLVSGY 190 200 210 220 230 240 FNAGFILINI PLWTAENISK KAIEMLKDPE VVQRITHLDQ DVLNIFLVNK ARFVDKKFNT 250 260 270 280 290 300 QFSLNYELKD SVINPVDAET VFVHYIGPTK PWHSWGAYPV SQYFLQAKSN SPWSHCALLN 310 320 330 PVTSHQLRYA AKHMFNQKHY TSGINYYIAY FKRKLLE

ReferencesHide

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of rfaI and rfaJ genes encoding lipopolysaccharide glycosyl transferases from Salmonella typhimurium." Carstenius P., Flock J.-I., Lindberg A. Nucleic Acids Res. 18:6128-6128(1990) [PubMed: 2235496] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"The assembly system for the lipopolysaccharide R2 core-type of Escherichia coli is a hybrid of those found in Escherichia coli K-12 and Salmonella enterica. Structure and function of the R2 WaaK and WaaL homologs." Heinrichs D.E., Monteiro M.A., Perry M.B., Whitfield C. J. Biol. Chem. 273:8849-8859(1998) [PubMed: 9535865] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[3]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-referencesHide

Sequence databases
EMBL GenBank DDBJ	X53847 Genomic DNA. Translation: CAA37841.1. Frameshift. AF026386 Genomic DNA. Translation: AAC16412.1. AE006468 Genomic DNA. Translation: AAL22577.1.
PIR	S12097.
RefSeq	NP_462618.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT8. Glycosyltransferase Family 8.
Proteomic databases
PRIDE	P19816.
Genome annotation databases
GeneID	1255242.
GenomeReviews	Gene locus STM3718 in contig AE006468_GR.
KEGG	stm:STM3718.
NMPDR	fig|99287.1.peg.3594.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG417202.
OMA	MLADKAV.
ProtClustDB	CLSK880743.
Enzyme and pathway databases
BioCyc	STYP99287:STM3718-MONOMER.
BRENDA	2.4.1.44. 2.
Family and domain databases
InterPro	IPR002495. Glyco_trans_8. IPR013645. Glyco_transf_8N. [Graphical view]
Pfam	PF01501. Glyco_transf_8. 1 hit. PF08437. Glyco_transf_8N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry informationHide

Entry name

RFAI_SALTY

Accession

Primary (citable) accession number: P19816
Secondary accession number(s): O68267

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	January 23, 2002
Last modified:	March 2, 2010
This is version 80 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)