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P19811

- RPOA_EAVBU

UniProt

P19811 - RPOA_EAVBU

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Protein
Replicase polyprotein 1ab
Gene
rep, 1a-1b
Organism
Equine arteritis virus (strain Bucyrus) (EAV)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

The replicase polyprotein 1ab is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.1 Publication
Nsp1 is essential for viral subgenomic mRNA synthesis.1 Publication
Nsp2 cysteine proteinase which cleaves the nsp2/nsp3 site in the polyprotein. Also displays deubiquitinating and deISGylase activities. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent host innate immunity.1 Publication
The 3C-like serine proteinase chain is responsible for the majority of cleavages as it cleaves the C-terminus of the polyprotein.1 Publication
The helicase chain, which contains a zinc finger structure, displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity.1 Publication

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
ATP + H2O = ADP + phosphate.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei164 – 1641For Nsp1 papain-like cysteine proteinase activity
Active sitei230 – 2301For Nsp1 papain-like cysteine proteinase activity
Sitei260 – 2612Cleavage; by PCP
Active sitei270 – 2701For Nsp2 cysteine proteinase activity
Active sitei332 – 3321For Nsp2 cysteine proteinase activity
Sitei831 – 8322Cleavage; by Nsp2 cysteine proteinase
Sitei1064 – 10652Cleavage; by 3CLSP
Active sitei1103 – 11031Charge relay system; for 3C-like serine proteinase activity By similarity
Active sitei1129 – 11291Charge relay system; for 3C-like serine proteinase activity By similarity
Active sitei1184 – 11841Charge relay system; for 3C-like serine proteinase activity By similarity
Sitei1268 – 12692Cleavage; by 3CLSP; in major pathway
Sitei1430 – 14312Cleavage; by 3CLSP; in minor pathway
Sitei1452 – 14532Cleavage; by 3CLSP; in minor pathway
Sitei1575 – 15762Cleavage; by 3CLSP
Sitei1677 – 16782Cleavage; by 3CLSP
Sitei2370 – 23712Cleavage; by 3CLSP
Sitei2429 – 24291Involved in mRNA transcription process
Sitei2837 – 28382Cleavage; by 3CLSP
Sitei3056 – 30572Cleavage; by 3CLSP

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri25 – 4420C4-type; atypical
Add
BLAST
Nucleotide bindingi2528 – 25358ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: InterPro
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  4. cysteine-type endopeptidase activity Source: InterPro
  5. helicase activity Source: UniProtKB-KW
  6. metal ion binding Source: UniProtKB-KW
  7. serine-type endopeptidase activity Source: InterPro
  8. serine-type exopeptidase activity Source: InterPro

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
  2. suppression by virus of host ISG15 activity Source: UniProtKB-KW
  3. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
  4. transcription, DNA-templated Source: InterPro
  5. viral RNA genome replication Source: InterPro
  6. viral protein processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Serine protease, Thiol protease, Transferase

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host ISG15 by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Viral immunoevasion, Viral RNA replication

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.21.114. 6985.

Protein family/group databases

MEROPSiC33.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein 1ab
Alternative name(s):
ORF1ab polyprotein
Cleaved into the following 14 chains:
Alternative name(s):
CP2
Short name:
CP
Non-structural protein 3
Short name:
Nsp3
3C-like serine proteinase (EC:3.4.21.-)
Short name:
3CLSP
Alternative name(s):
Nsp4
Non-structural protein 5-6-7
Short name:
Nsp5-6-7
Non-structural protein 5
Short name:
Nsp5
Non-structural protein 6
Short name:
Nsp6
Non-structural protein 7-alpha
Short name:
Nsp7-alpha
Non-structural protein 7-beta
Short name:
Nsp7-beta
Non-structural protein 8
Short name:
Nsp8
RNA-directed RNA polymerase (EC:2.7.7.48)
Short name:
Pol
Short name:
RdRp
Alternative name(s):
Nsp9
Helicase (EC:3.6.4.12, EC:3.6.4.13)
Short name:
Hel
Alternative name(s):
Nsp10
Non-structural protein 11
Short name:
Nsp11
Non-structural protein 12
Short name:
Nsp12
Gene namesi
Name:rep
ORF Names:1a-1b
OrganismiEquine arteritis virus (strain Bucyrus) (EAV)
Taxonomic identifieri299386 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesArteriviridaeArterivirus
Virus hostiEquidae (horses) [TaxID: 9788]
ProteomesiUP000000353: Genome

Subcellular locationi

Chain Nsp1 papain-like cysteine proteinase : Host nucleus. Host cytoplasm 2 Publications
Chain Nsp2 cysteine proteinase : Host membrane; Multi-pass membrane protein Reviewed prediction 2 Publications
Chain Non-structural protein 3 : Host membrane; Multi-pass membrane protein Reviewed prediction 2 Publications
Chain Non-structural protein 5-6-7 : Host membrane; Multi-pass membrane protein Reviewed prediction 2 Publications
Chain 3C-like serine proteinase : Host cytoplasm Reviewed prediction 2 Publications
Chain RNA-directed RNA polymerase : Host cytoplasmhost perinuclear region Reviewed prediction 2 Publications
Chain Helicase : Host cytoplasmhost perinuclear region Reviewed prediction 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei530 – 55021Helical; Reviewed prediction
Add
BLAST
Transmembranei551 – 57121Helical; Reviewed prediction
Add
BLAST
Transmembranei625 – 64521Helical; Reviewed prediction
Add
BLAST
Transmembranei829 – 84921Helical; Reviewed prediction
Add
BLAST
Transmembranei903 – 92321Helical; Reviewed prediction
Add
BLAST
Transmembranei935 – 95521Helical; Reviewed prediction
Add
BLAST
Transmembranei977 – 99721Helical; Reviewed prediction
Add
BLAST
Transmembranei1291 – 131121Helical; Reviewed prediction
Add
BLAST
Transmembranei1333 – 135321Helical; Reviewed prediction
Add
BLAST
Transmembranei1355 – 137521Helical; Reviewed prediction
Add
BLAST
Transmembranei1385 – 140521Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. host cell membrane Source: UniProtKB-SubCell
  2. host cell nucleus Source: UniProtKB-SubCell
  3. host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
  4. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Host nucleus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi25 – 251C → A: Complete loss of transcription. 1 Publication
Mutagenesisi44 – 441C → A: Complete loss of transcription. 1 Publication
Mutagenesisi164 – 1641C → G or S: Complete loss of PCP proteinase activity. 2 Publications
Mutagenesisi219 – 2191H → A, G or V: No effect. 1 Publication
Mutagenesisi230 – 2301H → A, G or V: Complete loss of PCP proteinase activity. 1 Publication
Mutagenesisi260 – 2601G → V: Complete loss of nsp1-nsp2 cleavage. 1 Publication
Mutagenesisi270 – 2701C → A, H, R or S: Complete loss of CP2 activity. 1 Publication
Mutagenesisi271 – 2711G → W: Complete loss of CP2 activity. 1 Publication
Mutagenesisi291 – 2911D → E or N: No effect. 1 Publication
Mutagenesisi295 – 2951D → E or N: No effect. 1 Publication
Mutagenesisi296 – 2961D → E or N: No effect. 1 Publication
Mutagenesisi297 – 2971E → D or Q: No effect. 1 Publication
Mutagenesisi319 – 3191C → A, H or P: Complete loss of CP2 activity. 1 Publication
Mutagenesisi332 – 3321H → C, I, N or Y: Complete loss of CP2 activity. 1 Publication
Mutagenesisi344 – 3441C → A: No effect. 1 Publication
Mutagenesisi344 – 3441C → H: Almost complete loss of CP2 activity. 1 Publication
Mutagenesisi349 – 3491C → A, H or S: Complete loss of CP2 activity. 1 Publication
Mutagenesisi354 – 3541C → A, H or P: Complete loss of CP2 activity. 1 Publication
Mutagenesisi356 – 3561C → A: Complete loss of CP2 activity. 1 Publication
Mutagenesisi356 – 3561C → H: No effect. 1 Publication
Mutagenesisi831 – 8311G → P: Complete loss of nsp2-nsp3 cleavage. 1 Publication
Mutagenesisi1064 – 10641E → P: Complete loss of nsp3-nsp4 cleavage. 1 Publication
Mutagenesisi1103 – 11031H → G or R: Complete loss of nsp3-nsp4, nsp4-nsp5 and nsp5-nsp6 cleavages. 1 Publication
Mutagenesisi1117 – 11171D → N or T: No effect. 1 Publication
Mutagenesisi1129 – 11291D → E: Complete loss of nsp3-nsp4 and nsp5-nsp6 cleavages. 1 Publication
Mutagenesisi1129 – 11291D → K or V: Complete loss of nsp3-nsp4, nsp4-nsp5 and nsp5-nsp6 cleavages. 1 Publication
Mutagenesisi1179 – 11791T → G or S: Partial loss of nsp4-nsp5 cleavage. 1 Publication
Mutagenesisi1179 – 11791T → N: Increased nsp5-nsp6 cleavage. 1 Publication
Mutagenesisi1184 – 11841S → C, F, I or T: Complete loss of nsp3-nsp4, nsp4-nsp5 and nsp5-nsp6 cleavages. 1 Publication
Mutagenesisi1198 – 11981H → L, R or Y: Complete loss of nsp4-nsp5 and nsp5-nsp6 cleavages. 1 Publication
Mutagenesisi1268 – 12681E → P: Complete loss of nsp3-nsp4 and nsp4-nsp5 cleavages. 2 Publications
Mutagenesisi1430 – 14301E → P: No effect. 1 Publication
Mutagenesisi1677 – 16771E → P: Complete loss of nsp5-nsp6 cleavage. 1 Publication
Mutagenesisi2351 – 23511D → P: No effect. 1 Publication
Mutagenesisi2370 – 23701E → P: Complete loss of nsp9-nsp10 cleavage. 1 Publication
Mutagenesisi2429 – 24291S → P: RNA can replicate efficiently but does not produce the subgenomic mRNAs required for structural protein expression. 1 Publication
Mutagenesisi2800 – 28001E → P: No effect. 1 Publication
Mutagenesisi2819 – 28191D → P: No effect. 1 Publication
Mutagenesisi2835 – 28351E → D, P or Q: Almost complete loss of nsp9-nsp10 cleavage. 1 Publication
Mutagenesisi2837 – 28371Q → D or N: No effect. 1 Publication
Mutagenesisi2837 – 28371Q → E: Increased nsp9-nsp10 cleavage. 1 Publication
Mutagenesisi2837 – 28371Q → P: Almost complete loss of nsp9-nsp10 cleavage. 1 Publication
Mutagenesisi3056 – 30561E → P: Complete loss of nsp10-nsp11 cleavage. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 31753175Replicase polyprotein 1ab
PRO_0000036619Add
BLAST
Chaini1 – 260260Nsp1 papain-like cysteine proteinase
PRO_0000036621Add
BLAST
Chaini261 – 831571Nsp2 cysteine proteinase
PRO_0000036622Add
BLAST
Chaini832 – 1064233Non-structural protein 3
PRO_0000036623Add
BLAST
Chaini1065 – 12682043C-like serine proteinase
PRO_0000036624Add
BLAST
Chaini1269 – 1677409Non-structural protein 5-6-7
PRO_0000036625Add
BLAST
Chaini1269 – 1430162Non-structural protein 5
PRO_0000423106Add
BLAST
Chaini1431 – 145222Non-structural protein 6
PRO_0000423107Add
BLAST
Chaini1453 – 1575123Non-structural protein 7-alpha
PRO_0000423108Add
BLAST
Chaini1576 – 1677102Non-structural protein 7-beta
PRO_0000423109Add
BLAST
Chaini1678 – 2370693RNA-directed RNA polymerase
PRO_0000036626Add
BLAST
Chaini1678 – 172750Non-structural protein 8
PRO_0000036627Add
BLAST
Chaini2371 – 2837467Helicase
PRO_0000036628Add
BLAST
Chaini2838 – 3056219Non-structural protein 11
PRO_0000036629Add
BLAST
Chaini3057 – 3175119Non-structural protein 12
PRO_0000036630Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi1501 – 15011N-linked (GlcNAc...); by host Inferred

Post-translational modificationi

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. There are two alternative pathways for processing. Either nsp4-5 is cleaved, which represents the major pathway or the nsp5-6 and nsp6-7 are processed, which represents the minor pathway. The major pathway occurs when nsp2 acts as cofactor for nsp4.5 Publications

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Nsp1 interacts with cellular transcription cofactor SND1/p100.1 Publication

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi270 – 2778
Turni278 – 2803
Beta strandi283 – 2864
Helixi290 – 2923
Helixi296 – 30510
Beta strandi309 – 3124
Beta strandi323 – 3297
Beta strandi332 – 3376
Helixi347 – 3515
Helixi369 – 37911
Turni380 – 3823
Beta strandi383 – 3864
Beta strandi1076 – 109217
Beta strandi1095 – 11017
Helixi1102 – 11054
Beta strandi1110 – 11156
Beta strandi1118 – 11236
Beta strandi1125 – 11273
Beta strandi1130 – 11356
Turni1137 – 11393
Beta strandi1154 – 11618
Beta strandi1164 – 11707
Helixi1181 – 11833
Beta strandi1187 – 11904
Beta strandi1193 – 120210
Helixi1203 – 12053
Beta strandi1206 – 12105
Beta strandi1216 – 12205
Helixi1224 – 12285
Beta strandi1233 – 12375
Beta strandi1250 – 12545
Helixi1255 – 12628
Turni1454 – 14596
Helixi1463 – 147412
Beta strandi1476 – 14805
Beta strandi1483 – 14864
Helixi1490 – 150617
Helixi1510 – 152011
Turni1521 – 15244
Beta strandi1533 – 15397
Beta strandi1545 – 15484
Beta strandi1551 – 156010
Beta strandi1565 – 15739
Turni2375 – 23773
Beta strandi2382 – 23854
Turni2393 – 23953
Helixi2396 – 23994
Beta strandi2400 – 24034
Beta strandi2405 – 24095
Helixi2416 – 24183
Beta strandi2419 – 24235
Turni2424 – 24285
Helixi2440 – 245112
Beta strandi2457 – 24626
Beta strandi2465 – 24684
Beta strandi2470 – 24756
Beta strandi2478 – 24836
Beta strandi2488 – 24947
Beta strandi2496 – 25038
Helixi2513 – 25219
Beta strandi2524 – 25274
Helixi2534 – 254411
Beta strandi2549 – 25524
Helixi2556 – 256813
Beta strandi2591 – 25966
Beta strandi2605 – 26095
Turni2610 – 26145
Helixi2617 – 262610
Beta strandi2630 – 26334
Turni2648 – 26514
Helixi2652 – 26543
Beta strandi2655 – 26573
Beta strandi2665 – 26673
Helixi2669 – 26746
Turni2675 – 26784
Beta strandi2690 – 26934
Beta strandi2702 – 27087
Helixi2709 – 27113
Beta strandi2717 – 27193
Helixi2720 – 27223
Beta strandi2727 – 27337
Beta strandi2736 – 27383
Helixi2742 – 27487
Beta strandi2750 – 275910
Helixi2765 – 27684

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MBMX-ray2.00A/B/C/D1071-1268[»]
2L8KNMR-A1454-1575[»]
4IUMX-ray1.45A261-392[»]
4N0NX-ray2.00A2371-2772[»]
4N0OX-ray2.65A/C/E/G2371-2772[»]
ProteinModelPortaliP19811.
SMRiP19811. Positions 1071-1268.

Miscellaneous databases

EvolutionaryTraceiP19811.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini66 – 15691Peptidase C31
Add
BLAST
Domaini157 – 260104Peptidase C32
Add
BLAST
Domaini261 – 360100Peptidase C33
Add
BLAST
Domaini1065 – 1268204Peptidase S32
Add
BLAST
Domaini2116 – 2251136RdRp catalytic
Add
BLAST
Domaini2371 – 243868AV MBD
Add
BLAST
Domaini2496 – 2661166(+)RNA virus helicase ATP-binding
Add
BLAST
Domaini2662 – 2793132(+)RNA virus helicase C-terminal
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni261 – 33979OTU-like
Add
BLAST
Regioni530 – 645116HD1
Add
BLAST
Regioni829 – 997169HD2
Add
BLAST
Regioni1291 – 1405115HD3
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi389 – 44052Pro-rich
Add
BLAST

Domaini

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.
The OTU-like region is responsible for the deubiquitinating and deISGylation activities of Nsp2 Inferred.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

Gene3Di3.30.40.20. 1 hit.
InterProiIPR027351. (+)RNA_virus_helicase_core_dom.
IPR008743. Arterivirus_Nsp2_C33.
IPR023338. Arterivirus_NSP4_peptidase.
IPR027355. AV_MBD_dom.
IPR008741. AV_PCPalpha.
IPR025773. AV_PCPbeta.
IPR023183. Chymotrypsin-like_domain3.
IPR022230. DUF3756.
IPR029323. EAV_nsp1.
IPR008760. EAV_peptidase_S32.
IPR027417. P-loop_NTPase.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF12581. DUF3756. 1 hit.
PF14754. IFR3_antag. 1 hit.
PF05412. Peptidase_C33. 1 hit.
PF05579. Peptidase_S32. 1 hit.
PF00680. RdRP_1. 1 hit.
PF01443. Viral_helicase1. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51538. AV_CP. 1 hit.
PS51652. AV_MBD. 1 hit.
PS51493. AV_NSP4_PRO. 1 hit.
PS51539. AV_PCP_ALPHA. 1 hit.
PS51540. AV_PCP_BETA. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

Isoform Replicase polyprotein 1ab (identifier: P19811-1) [UniParc]FASTAAdd to Basket

Also known as: pp1ab

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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MATFSATGFG GSFVRDWSLD LPDACEHGAG LCCEVDGSTL CAECFRGCEG     50
MEQCPGLFMG LLKLASPVPV GHKFLIGWYR AAKVTGRYNF LELLQHPAFA 100
QLRVVDARLA IEEASVFIST DHASAKRFPG ARFALTPVYA NAWVVSPAAN 150
SLIVTTDQEQ DGFCWLKLLP PDRREAGLRL YYNHYREQRT GWLSKTGLRL 200
WLGDLGLGIN ASSGGLKFHI MRGSPQRAWH ITTRSCKLKS YYVCDISEAD 250
WSCLPAGNYG GYNPPGDGAC GYRCLAFMNG ATVVSAGCSS DLWCDDELAY 300
RVFQLSPTFT VTIPGGRVCP NAKYAMICDK QHWRVKRAKG VGLCLDESCF 350
RGICNCQRMS GPPPAPVSAA VLDHILEAAT FGNVRVVTPE GQPRPVPAPR 400
VRPSANSSGD VKDPAPVPPV PKPRTKLATP NPTQAPIPAP RTRLQGASTQ 450
EPLASAGVAS DSAPKWRVAK TVYSSAERFR TELVQRARSV GDVLVQALPL 500
KTPAVQRYTM TLKMMRSRFS WHCDVWYPLA VIACLLPIWP SLALLLSFAI 550
GLIPSVGNNV VLTALLVSSA NYVASMDHQC EGAACLALLE EEHYYRAVRW 600
RPITGALSLV LNLLGQVGYV ARSTFDAAYV PCTVFDLCSF AILYLCRNRC 650
WRCFGRCVRV GPATHVLGST GQRVSKLALI DLCDHFSKPT IDVVGMATGW 700
SGCYTGTAAM ERQCASTVDP HSFDQKKAGA TVYLTPPVNS GSALQCLNVM 750
WKRPIGSTVL GEQTGAVVTA VKSISFSPPC CVSTTLPTRP GVTVVDHALY 800
NRLTASGVDP ALLRVGQGDF LKLNPGFRLI GGWIYGICYF VLVVVSTFTC 850
LPIKCGIGTR DPFCRRVFSV PVTKTQEHCH AGMCASAEGI SLDSLGLTQL 900
QSYWIAAVTS GLVILLVCHR LAISALDLLT LASPLVLLVF PWASVGLLLA 950
CSLAGAAVKI QLLATLFVNL FFPQATLVTM GYWACVAALA VYSLMGLRVK 1000
VNVPMCVTPA HFLLLARSAG QSREQMLRVS AAAPTNSLLG VARDCYVTGT 1050
TRLYIPKEGG MVFEGLFRSP KARGNVGFVA GSSYGTGSVW TRNNEVVVLT 1100
ASHVVGRANM ATLKIGDAML TLTFKKNGDF AEAVTTQSEL PGNWPQLHFA 1150
QPTTGPASWC TATGDEEGLL SGEVCLAWTT SGDSGSAVVQ GDAVVGVHTG 1200
SNTSGVAYVT TPSGKLLGAD TVTLSSLSKH FTGPLTSIPK DIPDNIIADV 1250
DAVPRSLAML IDGLSNRESS LSGPQLLLIA CFMWSYLNQP AYLPYVLGFF 1300
AANFFLPKSV GRPVVTGLLW LCCLFTPLSM RLCLFHLVCA TVTGNVISLW 1350
FYITAAGTSY LSEMWFGGYP TMLFVPRFLV YQFPGWAIGT VLAVCSITML 1400
AAALGHTLLL DVFSASGRFD RTFMMKYFLE GGVKESVTAS VTRAYGKPIT 1450
QESLTATLAA LTDDDFQFLS DVLDCRAVRS AMNLRAALTS FQVAQYRNIL 1500
NASLQVDRDA ARSRRLMAKL ADFAVEQEVT AGDRVVVIDG LDRMAHFKDD 1550
LVLVPLTTKV VGGSRCTICD VVKEEANDTP VKPMPSRRRR KGLPKGAQLE 1600
WDRHQEEKRN AGDDDFAVSN DYVKRVPKYW DPSDTRGTTV KIAGTTYQKV 1650
VDYSGNVHYV EHQEDLLDYV LGKGSYEGLD QDKVLDLTNM LKVDPTELSS 1700
KDKAKARQLA HLLLDLANPV EAVNQLNLRA PHIFPGDVGR RTFADSKDKG 1750
FVALHSRTMF LAARDFLFNI KFVCDEEFTK TPKDTLLGYV RACPGYWFIF 1800
RRTHRSLIDA YWDSMECVYA LPTISDFDVS PGDVAVTGER WDFESPGGGR 1850
AKRLTADLVH AFQGFHGASY SYDDKVAAAV SGDPYRSDGV LYNTRWGNIP 1900
YSVPTNALEA TACYRAGCEA VTDGTNVIAT IGPFPEQQPI PDIPKSVLDN 1950
CADISCDAFI APAAETALCG DLEKYNLSTQ GFVLPSVFSM VRAYLKEEIG 2000
DAPPLYLPST VPSKNSQAGI NGAEFPTKSL QSYCLIDDMV SQSMKSNLQT 2050
ATMATCKRQY CSKYKIRSIL GTNNYIGLGL RACLSGVTAA FQKAGKDGSP 2100
IYLGKSKFDP IPAPDKYCLE TDLESCDRST PALVRWFATN LIFELAGQPE 2150
LVHSYVLNCC HDLVVAGSVA FTKRGGLSSG DPITSISNTI YSLVLYTQHM 2200
LLCGLEGYFP EIAEKYLDGS LELRDMFKYV RVYIYSDDVV LTTPNQHYAA 2250
SFDRWVPHLQ ALLGFKVDPK KTVNTSSPSF LGCRFKQVDG KCYLASLQDR 2300
VTRSLLYHIG AKNPSEYYEA AVSIFKDSII CCDEDWWTDL HRRISGAART 2350
DGVEFPTIEM LTSFRTKQYE SAVCTVCGAA PVAKSACGGW FCGNCVPYHA 2400
GHCHTTSLFA NCGHDIMYRS TYCTMCEGSP KQMVPKVPHP ILDHLLCHID 2450
YGSKEELTLV VADGRTTSPP GRYKVGHKVV AVVADVGGNI VFGCGPGSHI 2500
AVPLQDTLKG VVVNKALKNA AASEYVEGPP GSGKTFHLVK DVLAVVGSAT 2550
LVVPTHASML DCINKLKQAG ADPYFVVPKY TVLDFPRPGS GNITVRLPQV 2600
GTSEGETFVD EVAYFSPVDL ARILTQGRVK GYGDLNQLGC VGPASVPRNL 2650
WLRHFVSLEP LRVCHRFGAA VCDLIKGIYP YYEPAPHTTK VVFVPNPDFE 2700
KGVVITAYHK DRGLGHRTID SIQGCTFPVV TLRLPTPQSL TRPRAVVAVT 2750
RASQELYIYD PFDQLSGLLK FTKEAEAQDL IHGPPTACHL GQEIDLWSNE 2800
GLEYYKEVNL LYTHVPIKDG VIHSYPNCGP ACGWEKQSNK ISCLPRVAQN 2850
LGYHYSPDLP GFCPIPKELA EHWPVVSNDR YPNCLQITLQ QVCELSKPCS 2900
AGYMVGQSVF VQTPGVTSYW LTEWVDGKAR ALPDSLFSSG RFETNSRAFL 2950
DEAEEKFAAA HPHACLGEIN KSTVGGSHFI FSQYLPPLLP ADAVALVGAS 3000
LAGKAAKAAC SVVDVYAPSF EPYLHPETLS RVYKIMIDFK PCRLMVWRNA 3050
TFYVQEGVDA VTSALAAVSK LIKVPANEPV SFHVASGYRT NALVAPQAKI 3100
SIGAYAAEWA LSTEPPPAGY AIVRRYIVKR LLSSTEVFLC RRGVVSSTSV 3150
QTICALEGCK PLFNFLQIGS VIGPV 3175

Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

Length:3,175
Mass (Da):345,384
Last modified:April 13, 2004 - v3
Checksum:iCB536C1F5091045C
GO
Isoform Replicase polyprotein 1a (identifier: P19811-2) [UniParc]FASTAAdd to Basket

Also known as: pp1a, ORF1a polyprotein

The sequence of this isoform differs from the canonical sequence as follows:
     1728-3175: Missing.

Note: Produced by conventional translation.

Show »
Length:1,727
Mass (Da):186,992
Checksum:iFB0DB046FA0C74F6
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1728 – 31751448Missing in isoform Replicase polyprotein 1a.
VSP_032887Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53459 Genomic RNA. Translation: CAC42774.2.
X53459 Genomic RNA. Translation: CAC42775.2.
X52277 Genomic RNA. Translation: CAA36520.1.
PIRiA39925. RRWVEV.
RefSeqiNP_127506.1. NC_002532.2.
NP_127507.1. NC_002532.2.

Genome annotation databases

GeneIDi921339.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53459 Genomic RNA. Translation: CAC42774.2 .
X53459 Genomic RNA. Translation: CAC42775.2 .
X52277 Genomic RNA. Translation: CAA36520.1 .
PIRi A39925. RRWVEV.
RefSeqi NP_127506.1. NC_002532.2.
NP_127507.1. NC_002532.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1MBM X-ray 2.00 A/B/C/D 1071-1268 [» ]
2L8K NMR - A 1454-1575 [» ]
4IUM X-ray 1.45 A 261-392 [» ]
4N0N X-ray 2.00 A 2371-2772 [» ]
4N0O X-ray 2.65 A/C/E/G 2371-2772 [» ]
ProteinModelPortali P19811.
SMRi P19811. Positions 1071-1268.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C33.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 921339.

Enzyme and pathway databases

BRENDAi 3.4.21.114. 6985.

Miscellaneous databases

EvolutionaryTracei P19811.

Family and domain databases

Gene3Di 3.30.40.20. 1 hit.
InterProi IPR027351. (+)RNA_virus_helicase_core_dom.
IPR008743. Arterivirus_Nsp2_C33.
IPR023338. Arterivirus_NSP4_peptidase.
IPR027355. AV_MBD_dom.
IPR008741. AV_PCPalpha.
IPR025773. AV_PCPbeta.
IPR023183. Chymotrypsin-like_domain3.
IPR022230. DUF3756.
IPR029323. EAV_nsp1.
IPR008760. EAV_peptidase_S32.
IPR027417. P-loop_NTPase.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF12581. DUF3756. 1 hit.
PF14754. IFR3_antag. 1 hit.
PF05412. Peptidase_C33. 1 hit.
PF05579. Peptidase_S32. 1 hit.
PF00680. RdRP_1. 1 hit.
PF01443. Viral_helicase1. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEi PS51538. AV_CP. 1 hit.
PS51652. AV_MBD. 1 hit.
PS51493. AV_NSP4_PRO. 1 hit.
PS51539. AV_PCP_ALPHA. 1 hit.
PS51540. AV_PCP_BETA. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Equine arteritis virus is not a togavirus but belongs to the coronaviruslike superfamily."
    den Boon J.A., Snijder E.J., Chirnside E.D., de Vries A.A.F., Horzinek M.C., Spaan W.J.M.
    J. Virol. 65:2910-2920(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. Snijder E.J.
    Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "All subgenomic mRNAs of equine arteritis virus contain a common leader sequence."
    de Vries A.A.F., Chirnside E.D., Bredenbeek P.J., Gravestein L.A., Horzinek M.C., Spaan W.J.M.
    Nucleic Acids Res. 18:3241-3247(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-17.
  4. "The 5' end of the equine arteritis virus replicase gene encodes a papainlike cysteine protease."
    Snijder E.J., Wassenaar A.L.M., Spaan W.J.M.
    J. Virol. 66:7040-7048(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN 1A, CHARACTERIZATION OF PCP, MUTAGENESIS OF CYS-164; HIS-219; HIS-230 AND GLY-260.
  5. "The arterivirus Nsp2 protease. An unusual cysteine protease with primary structure similarities to both papain-like and chymotrypsin-like proteases."
    Snijder E.J., Wassenaar A.L.M., Spaan W.J.M., Gorbalenya A.E.
    J. Biol. Chem. 270:16671-16676(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF NSP2, MUTAGENESIS OF CYS-270; GLY-271; ASP-291; ASP-295; ASP-296; GLU-297; CYS-319; HIS-332; CYS-344; CYS-349; CYS-354 AND CYS-356.
  6. "The arterivirus nsp4 protease is the prototype of a novel group of chymotrypsin-like enzymes, the 3C-like serine proteases."
    Snijder E.J., Wassenaar A.L.M., van Dinten L.C., Spaan W.J.M., Gorbalenya A.E.
    J. Biol. Chem. 271:4864-4871(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN 1A, CHARACTERIZATION OF 3CLSP, MUTAGENESIS OF CYS-164; GLY-831; GLU-1064; HIS-1103; ASP-1117; ASP-1129; THR-1179; SER-1184; HIS-1198; GLU-1268; GLU-1430 AND GLU-1677.
  7. "An infectious arterivirus cDNA clone: identification of a replicase point mutation that abolishes discontinuous mRNA transcription."
    van Dinten L.C., den Boon J.A., Wassenaar A.L.M., Spaan W.J.M., Snijder E.J.
    Proc. Natl. Acad. Sci. U.S.A. 94:991-996(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF SER-2429.
  8. "Alternative proteolytic processing of the arterivirus replicase ORF1a polyprotein: evidence that NSP2 acts as a cofactor for the NSP4 serine protease."
    Wassenaar A.L.M., Spaan W.J.M., Gorbalenya A.E., Snijder E.J.
    J. Virol. 71:9313-9322(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN 1A, MUTAGENESIS OF GLU-1268.
  9. "ORF1a-encoded replicase subunits are involved in the membrane association of the arterivirus replication complex."
    van der Meer Y., van Tol H., Locker J.K., Snijder E.J.
    J. Virol. 72:6689-6698(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  10. "Proteolytic processing of the open reading frame 1b-encoded part of arterivirus replicase is mediated by nsp4 serine protease and is essential for virus replication."
    van Dinten L.C., Rensen S., Gorbalenya A.E., Snijder E.J.
    J. Virol. 73:2027-2037(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN 1B, MUTAGENESIS OF ASP-2351; GLU-2370; GLU-2800; ASP-2819; GLU-2835; GLN-2837 AND GLU-3056.
  11. "Biochemical characterization of the equine arteritis virus helicase suggests a close functional relationship between arterivirus and coronavirus helicases."
    Seybert A., van Dinten L.C., Snijder E.J., Ziebuhr J.
    J. Virol. 74:9586-9593(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF HELICASE.
  12. "A zinc finger-containing papain-like protease couples subgenomic mRNA synthesis to genome translation in a positive-stranded RNA virus."
    Tijms M.A., van Dinten L.C., Gorbalenya A.E., Snijder E.J.
    Proc. Natl. Acad. Sci. U.S.A. 98:1889-1894(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: C4-TYPE ZINC-FINGER OF NSP1, MUTAGENESIS OF CYS-25 AND CYS-44.
  13. "Nuclear localization of non-structural protein 1 and nucleocapsid protein of equine arteritis virus."
    Tijms M.A., van der Meer Y., Snijder E.J.
    J. Gen. Virol. 83:795-800(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION OF NSP1 PAPAIN-LIKE CYSTEINE PROTEINASE.
  14. "Equine arteritis virus non-structural protein 1, an essential factor for viral subgenomic mRNA synthesis, interacts with the cellular transcription co-factor p100."
    Tijms M.A., Snijder E.J.
    J. Gen. Virol. 84:2317-2322(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HUMAN SND1/P100.
  15. Cited for: FUNCTION OF NSP2.
  16. "Identification of porcine reproductive and respiratory syndrome virus ORF1a-encoded non-structural proteins in virus-infected cells."
    Li Y., Tas A., Snijder E.J., Fang Y.
    J. Gen. Virol. 93:829-839(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN 1A, PROTEOLYTIC PROCESSING OF POLYPROTEIN 1B.
  17. "Structure of arterivirus nsp4. The smallest chymotrypsin-like proteinase with an alpha/beta C-terminal extension and alternate conformations of the oxyanion hole."
    Barrette-Ng I.H., Ng K.K.-S., Mark B.L., Van Aken D., Cherney M.M., Garen C., Kolodenko Y., Gorbalenya A.E., Snijder E.J., James M.N.G.
    J. Biol. Chem. 277:39960-39966(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1071-1268 (NSP4).

Entry informationi

Entry nameiRPOA_EAVBU
AccessioniPrimary (citable) accession number: P19811
Secondary accession number(s): Q88625, Q8QZQ5, Q91DM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: April 13, 2004
Last modified: June 11, 2014
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Nsp1 contains an inactivated papain-like cysteine proteinase domain due to a Lys instead of a Cys in position 73.

Keywords - Technical termi

3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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