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P19809 (EAE_ECO27) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Intimin
Alternative name(s):
Attaching and effacing protein
Short name=Eae protein
Gene names
Name:eae
Synonyms:eaeA
Ordered Locus Names:E2348C_3939
OrganismEscherichia coli O127:H6 (strain E2348/69 / EPEC) [Complete proteome] [HAMAP]
Taxonomic identifier574521 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length939 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Necessary for the production of attaching and effacing lesions on tissue culture cells. Believed to mediate adherence.

Subcellular location

Cell outer membrane; Single-pass membrane protein.

Sequence similarities

Belongs to the intimin/invasin family.

Contains 2 Big-1 (bacterial Ig-like domain 1) domains.

Contains 1 LysM repeat.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

tirQ9KWH91EBI-1029391,EBI-1029383From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 939939Intimin
PRO_0000211827

Regions

Transmembrane20 – 3920Helical; Potential
Repeat65 – 11349LysM
Domain560 – 65394Big-1 1
Domain660 – 75192Big-1 2

Experimental info

Sequence conflict1051A → E in AAA62775. Ref.1
Sequence conflict1051A → E in AAC38392. Ref.2

Secondary structure

1 ................................................................. 939
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P19809 [UniParc].

Last modified May 5, 2009. Version 2.
Checksum: C42BC5C0A965B0BD

FASTA939102,353
        10         20         30         40         50         60 
MITHGFYART RHKHKLKKTF IMLSAGLGLF FYVNQNSFAN GENYFKLGSD SKLLTHNSYQ 

        70         80         90        100        110        120 
NRLFYTLKTG ETVADLSKSQ DINLSTIWSL NKHLYSSESE MMKAAPGQQI ILPLKKLPFE 

       130        140        150        160        170        180 
YSALPLLGSA PLVAAGGVAG HTNKLTKMSP DVTKSNMTDD KALNYAAQQA ASLGSQLQSR 

       190        200        210        220        230        240 
SLNGDYAKDT ALGIAGNQAS SQLQAWLQHY GTAEVNLQSG NNFDGSSLDF LLPFYDSEKM 

       250        260        270        280        290        300 
LAFGQVGARY IDSRFTANLG AGQRFFLPEN MLGYNVFIDQ DFSGDNTRLG IGGEYWRDYF 

       310        320        330        340        350        360 
KSSVNGYFRM SGWHESYNKK DYDERPANGF DIRFNGYLPS YPALGAKLMY EQYYGDNVAL 

       370        380        390        400        410        420 
FNSDKLQSNP GAATVGVNYT PIPLVTMGID YRHGTGNEND LLYSMQFRYQ FDKPWSQQIE 

       430        440        450        460        470        480 
PQYVNELRTL SGSRYDLVQR NNNIILEYKK QDILSLNIPH DINGTERSTQ KIQLIVKSKY 

       490        500        510        520        530        540 
GLDRIVWDDS ALRSQGGQIQ HSGSQSAQDY QAILPAYVQG GSNVYKVTAR AYDRNGNSSN 

       550        560        570        580        590        600 
NVLLTITVLS NGQVVDQVGV TDFTADKTSA KADGTEAITY TATVKKNGVA QANVPVSFNI 

       610        620        630        640        650        660 
VSGTAVLSAN SANTNGSGKA TVTLKSDKPG QVVVSAKTAE MTSALNANAV IFVDQTKASI 

       670        680        690        700        710        720 
TEIKADKTTA VANGQDAITY TVKVMKGDKP VSNQEVTFTT TLGKLSNSTE KTDTNGYAKV 

       730        740        750        760        770        780 
TLTSTTPGKS LVSARVSDVA VDVKAPEVEF FTTLTIDDGN IEIVGTGVKG KLPTVWLQYG 

       790        800        810        820        830        840 
QVNLKASGGN GKYTWRSANP AIASVDASSG QVTLKEKGTT TISVISSDNQ TATYTIATPN 

       850        860        870        880        890        900 
SLIVPNMSKR VTYNDAVNTC KNFGGKLPSS QNELENVFKA WGAANKYEYY KSSQTIISWV 

       910        920        930 
QQTAQDAKSG VASTYDLVKQ NPLNNIKASE SNAYATCVK

« Hide

References

« Hide 'large scale' references
[1]"A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells."
Jerse A.E., Yu J., Tall B.D., Kaper J.B.
Proc. Natl. Acad. Sci. U.S.A. 87:7839-7843(1990) [PubMed: 2172966] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: E2348/69 / EPEC.
[2]"The complete sequence of the locus of enterocyte effacement (LEE) from enteropathogenic Escherichia coli E2348/69."
Elliott S.J., Wainwright L.A., McDaniel T.K., Jarvis K.G., Deng Y.K., Lai L.C., McNamara B.P., Donnenberg M.S., Kaper J.B.
Mol. Microbiol. 28:1-4(1998) [PubMed: 9593291] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: E2348/69 / EPEC.
[3]"Complete genome sequence and comparative genome analysis of enteropathogenic Escherichia coli O127:H6 strain E2348/69."
Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T., Henderson I.R., Harris D., Asadulghani M., Kurokawa K., Dean P., Kenny B., Quail M.A., Thurston S., Dougan G., Hayashi T., Parkhill J., Frankel G.
J. Bacteriol. 191:347-354(2009) [PubMed: 18952797] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: E2348/69 / EPEC.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M58154 Genomic DNA. Translation: AAA62775.1.
AF022236 Genomic DNA. Translation: AAC38392.1.
FM180568 Genomic DNA. Translation: CAS11487.1.
PIRI41197.
RefSeqYP_002331401.1. NC_011601.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1E5UNMR-I753-939[»]
1F00X-ray1.90I658-939[»]
1F02X-ray2.90I658-939[»]
ProteinModelPortalP19809.
SMRP19809. Positions 566-939.
ModBaseSearch...

Protein-protein interaction databases

IntActP19809. 1 interaction.
STRINGP19809.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000112066; EBESCP00000106277; EBESCG00000115379.
GeneID7063868.
GenomeReviewsGene locus E2348_C_3939 in contig FM180568_GR.
KEGGecg:E2348C_3939.
PATRIC18347094. VBIEscCol90278_4030.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000008359.
HOGENOMHBG468012.
OMAFFLPENM.
ProtClustDBCLSK334542.

Family and domain databases

InterProIPR003344. Big_1.
IPR003343. Big_2.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
IPR024519. DUF3442_intimin/invasin.
IPR003535. Intimin/invasin_bac.
IPR013117. Intimin_C.
IPR008964. Invasin/intimin_cell_adhesion.
IPR018392. Peptidoglycan-bd_lysin.
IPR002482. Peptidoglycan-bd_Lysin_subgr.
[Graphical view]
Gene3DG3DSA:2.60.40.920. Big_1. 2 hits.
G3DSA:3.10.100.10. C-type_lectin-like. 1 hit.
KOK12790.
PfamPF02369. Big_1. 2 hits.
PF02368. Big_2. 1 hit.
PF11924. DUF3442. 1 hit.
PF07979. Intimin_C. 1 hit.
PF01476. LysM. 1 hit.
[Graphical view]
PRINTSPR01369. INTIMIN.
SMARTSM00634. BID_1. 2 hits.
SM00635. BID_2. 1 hit.
SM00257. LysM. 1 hit.
[Graphical view]
SUPFAMSSF56436. C-type_lectin_fold. 1 hit.
SSF49373. Invasin_intimin. 3 hits.
PROSITEPS51127. BIG1. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameEAE_ECO27
AccessionPrimary (citable) accession number: P19809
Secondary accession number(s): B7UM97
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: May 5, 2009
Last modified: January 25, 2012
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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