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Protein

Intimin

Gene

eae

Organism
Escherichia coli O127:H6 (strain E2348/69 / EPEC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for the production of attaching and effacing lesions on tissue culture cells. Believed to mediate adherence.

GO - Biological processi

  • cell adhesion Source: InterPro
  • pathogenesis Source: CACAO
Complete GO annotation...

Keywords - Biological processi

Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Intimin
Alternative name(s):
Attaching and effacing protein
Short name:
Eae protein
Gene namesi
Name:eae
Synonyms:eaeA
Ordered Locus Names:E2348C_3939
OrganismiEscherichia coli O127:H6 (strain E2348/69 / EPEC)
Taxonomic identifieri574521 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000008205 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei20 – 39HelicalSequence analysisAdd BLAST20

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002118271 – 939IntiminAdd BLAST939

Interactioni

Protein-protein interaction databases

IntActiP19809. 1 interactor.

Structurei

Secondary structure

1939
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi41 – 43Combined sources3
Beta strandi50 – 52Combined sources3
Helixi54 – 61Combined sources8
Beta strandi62 – 66Combined sources5
Helixi73 – 80Combined sources8
Helixi84 – 91Combined sources8
Turni92 – 94Combined sources3
Beta strandi95 – 97Combined sources3
Helixi98 – 102Combined sources5
Beta strandi109 – 113Combined sources5
Beta strandi118 – 121Combined sources4
Beta strandi141 – 143Combined sources3
Beta strandi659 – 666Combined sources8
Beta strandi668 – 674Combined sources7
Beta strandi678 – 686Combined sources9
Beta strandi695 – 706Combined sources12
Beta strandi708 – 711Combined sources4
Beta strandi716 – 723Combined sources8
Beta strandi728 – 730Combined sources3
Beta strandi732 – 736Combined sources5
Beta strandi748 – 751Combined sources4
Beta strandi761 – 763Combined sources3
Turni764 – 766Combined sources3
Beta strandi769 – 771Combined sources3
Beta strandi774 – 777Combined sources4
Beta strandi781 – 783Combined sources3
Beta strandi786 – 788Combined sources3
Beta strandi790 – 792Combined sources3
Beta strandi793 – 798Combined sources6
Turni800 – 802Combined sources3
Beta strandi803 – 805Combined sources3
Turni807 – 809Combined sources3
Beta strandi811 – 814Combined sources4
Beta strandi816 – 818Combined sources3
Beta strandi820 – 826Combined sources7
Turni827 – 829Combined sources3
Beta strandi830 – 836Combined sources7
Beta strandi840 – 851Combined sources12
Helixi853 – 862Combined sources10
Beta strandi864 – 866Combined sources3
Helixi871 – 881Combined sources11
Helixi884 – 886Combined sources3
Helixi888 – 890Combined sources3
Beta strandi897 – 899Combined sources3
Helixi904 – 909Combined sources6
Beta strandi911 – 916Combined sources6
Turni917 – 919Combined sources3
Beta strandi920 – 927Combined sources8
Beta strandi928 – 930Combined sources3
Beta strandi933 – 938Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E5UNMR-I753-939[»]
1F00X-ray1.90I658-939[»]
1F02X-ray2.90I658-939[»]
2MPWNMR-A39-143[»]
ProteinModelPortaliP19809.
SMRiP19809.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP19809.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 112LysMPROSITE-ProRule annotationAdd BLAST50
Domaini560 – 653Big-1 1PROSITE-ProRule annotationAdd BLAST94
Domaini660 – 751Big-1 2PROSITE-ProRule annotationAdd BLAST92

Sequence similaritiesi

Belongs to the intimin/invasin family.Curated
Contains 1 LysM domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK12790.
OMAiPENMLGY.

Family and domain databases

Gene3Di2.60.40.920. 2 hits.
3.10.100.10. 1 hit.
InterProiIPR003344. Big_1_dom.
IPR003343. Big_2.
IPR016186. C-type_lectin-like/link.
IPR016187. CTDL_fold.
IPR024519. IAT_beta.
IPR003535. Intimin/invasin_bac.
IPR013117. Intimin_C.
IPR008964. Invasin/intimin_cell_adhesion.
IPR018392. LysM_dom.
[Graphical view]
PfamiPF02369. Big_1. 1 hit.
PF11924. IAT_beta. 1 hit.
PF07979. Intimin_C. 1 hit.
PF01476. LysM. 1 hit.
[Graphical view]
PRINTSiPR01369. INTIMIN.
SMARTiSM00634. BID_1. 2 hits.
SM00635. BID_2. 1 hit.
SM00257. LysM. 1 hit.
[Graphical view]
SUPFAMiSSF49373. SSF49373. 3 hits.
SSF56436. SSF56436. 1 hit.
PROSITEiPS51127. BIG1. 2 hits.
PS51782. LYSM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19809-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITHGFYART RHKHKLKKTF IMLSAGLGLF FYVNQNSFAN GENYFKLGSD
60 70 80 90 100
SKLLTHNSYQ NRLFYTLKTG ETVADLSKSQ DINLSTIWSL NKHLYSSESE
110 120 130 140 150
MMKAAPGQQI ILPLKKLPFE YSALPLLGSA PLVAAGGVAG HTNKLTKMSP
160 170 180 190 200
DVTKSNMTDD KALNYAAQQA ASLGSQLQSR SLNGDYAKDT ALGIAGNQAS
210 220 230 240 250
SQLQAWLQHY GTAEVNLQSG NNFDGSSLDF LLPFYDSEKM LAFGQVGARY
260 270 280 290 300
IDSRFTANLG AGQRFFLPEN MLGYNVFIDQ DFSGDNTRLG IGGEYWRDYF
310 320 330 340 350
KSSVNGYFRM SGWHESYNKK DYDERPANGF DIRFNGYLPS YPALGAKLMY
360 370 380 390 400
EQYYGDNVAL FNSDKLQSNP GAATVGVNYT PIPLVTMGID YRHGTGNEND
410 420 430 440 450
LLYSMQFRYQ FDKPWSQQIE PQYVNELRTL SGSRYDLVQR NNNIILEYKK
460 470 480 490 500
QDILSLNIPH DINGTERSTQ KIQLIVKSKY GLDRIVWDDS ALRSQGGQIQ
510 520 530 540 550
HSGSQSAQDY QAILPAYVQG GSNVYKVTAR AYDRNGNSSN NVLLTITVLS
560 570 580 590 600
NGQVVDQVGV TDFTADKTSA KADGTEAITY TATVKKNGVA QANVPVSFNI
610 620 630 640 650
VSGTAVLSAN SANTNGSGKA TVTLKSDKPG QVVVSAKTAE MTSALNANAV
660 670 680 690 700
IFVDQTKASI TEIKADKTTA VANGQDAITY TVKVMKGDKP VSNQEVTFTT
710 720 730 740 750
TLGKLSNSTE KTDTNGYAKV TLTSTTPGKS LVSARVSDVA VDVKAPEVEF
760 770 780 790 800
FTTLTIDDGN IEIVGTGVKG KLPTVWLQYG QVNLKASGGN GKYTWRSANP
810 820 830 840 850
AIASVDASSG QVTLKEKGTT TISVISSDNQ TATYTIATPN SLIVPNMSKR
860 870 880 890 900
VTYNDAVNTC KNFGGKLPSS QNELENVFKA WGAANKYEYY KSSQTIISWV
910 920 930
QQTAQDAKSG VASTYDLVKQ NPLNNIKASE SNAYATCVK
Length:939
Mass (Da):102,353
Last modified:May 5, 2009 - v2
Checksum:iC42BC5C0A965B0BD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti105A → E in AAA62775 (PubMed:2172966).Curated1
Sequence conflicti105A → E in AAC38392 (PubMed:9593291).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58154 Genomic DNA. Translation: AAA62775.1.
AF022236 Genomic DNA. Translation: AAC38392.1.
FM180568 Genomic DNA. Translation: CAS11487.1.
PIRiI41197.
RefSeqiWP_000627890.1. NC_011601.1.

Genome annotation databases

EnsemblBacteriaiCAS11487; CAS11487; E2348C_3939.
KEGGiecg:E2348C_3939.
PATRICi18347094. VBIEscCol90278_4030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58154 Genomic DNA. Translation: AAA62775.1.
AF022236 Genomic DNA. Translation: AAC38392.1.
FM180568 Genomic DNA. Translation: CAS11487.1.
PIRiI41197.
RefSeqiWP_000627890.1. NC_011601.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E5UNMR-I753-939[»]
1F00X-ray1.90I658-939[»]
1F02X-ray2.90I658-939[»]
2MPWNMR-A39-143[»]
ProteinModelPortaliP19809.
SMRiP19809.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP19809. 1 interactor.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAS11487; CAS11487; E2348C_3939.
KEGGiecg:E2348C_3939.
PATRICi18347094. VBIEscCol90278_4030.

Phylogenomic databases

KOiK12790.
OMAiPENMLGY.

Miscellaneous databases

EvolutionaryTraceiP19809.

Family and domain databases

Gene3Di2.60.40.920. 2 hits.
3.10.100.10. 1 hit.
InterProiIPR003344. Big_1_dom.
IPR003343. Big_2.
IPR016186. C-type_lectin-like/link.
IPR016187. CTDL_fold.
IPR024519. IAT_beta.
IPR003535. Intimin/invasin_bac.
IPR013117. Intimin_C.
IPR008964. Invasin/intimin_cell_adhesion.
IPR018392. LysM_dom.
[Graphical view]
PfamiPF02369. Big_1. 1 hit.
PF11924. IAT_beta. 1 hit.
PF07979. Intimin_C. 1 hit.
PF01476. LysM. 1 hit.
[Graphical view]
PRINTSiPR01369. INTIMIN.
SMARTiSM00634. BID_1. 2 hits.
SM00635. BID_2. 1 hit.
SM00257. LysM. 1 hit.
[Graphical view]
SUPFAMiSSF49373. SSF49373. 3 hits.
SSF56436. SSF56436. 1 hit.
PROSITEiPS51127. BIG1. 2 hits.
PS51782. LYSM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEAE_ECO27
AccessioniPrimary (citable) accession number: P19809
Secondary accession number(s): B7UM97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: May 5, 2009
Last modified: November 30, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.