Reviewed, UniProtKB/Swiss-Prot P19805 (PGLR2_ASPTU)

Names and origin

Protein names

Recommended name:
    Polygalacturonase-2
    EC=3.2.1.15
Alternative name(s):
    Polygalacturonase II
      Short name=PG-II
    Pectinase-2

Gene names

Name:

pgaII

Organism

Aspergillus tubingensis

Taxonomic identifier

5068 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall.
Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 5 PbH1 repeats.
Caution	Strain RH 5344 was previously said to be from A.niger.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Propeptide	22 – 27	6	Potential		PRO_0000024778
	Chain	28 – 362	335	Polygalacturonase-2		PRO_0000024779
Sites
	Active site	201	1	Proton donor By similarity
	Active site	223	1	By similarity
Amino acid modifications
	Glycosylation	240	1	N-linked (GlcNAc...) Probable
	Disulfide bond	30 ↔ 45		By similarity
	Disulfide bond	203 ↔ 219		By similarity
	Disulfide bond	329 ↔ 334		By similarity
	Disulfide bond	353 ↔ 362		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P19805-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: AA4AC029DD1E6DEA	FASTA	362	37,513
	10 20 30 40 50 60 MHSFASLLAY GLAASATLAS ASPIEARGSC TFKTAAAAKA GKAGCSTITL DNIEVPAGTT 70 80 90 100 110 120 LDLTGLTSGT KVIFEGTTTF DYEEWAGPLI SMSGKDITVT GASGHLINCD GARWWDGKGT 130 140 150 160 170 180 SGKKKPKFFY AHGLDSSSIT GLNIKNTPLM AFSVQADDIT LTDITINNAD GDTLGGHNTD 190 200 210 220 230 240 AFDVGNSVGV NIIKPWVHNQ DDCLAINSGE NIWFTSGTCI GGHGLSIGSV GGRSNNVVKN 250 260 270 280 290 300 VTIEHSTVSN SENAVRIKTV SGATGSVSEI TYSNIVMSGI SDYGVVIQQD YEDGKPTGKP 310 320 330 340 350 360 TNGVTITDVK LESVTGTVDS KATDIYLLCG SGSCSDWTWD DVKVTGGKKS TACKNYPSVA SC

References

[1]	"Expression and sequence comparison of the Aspergillus niger and Aspergillus tubigensis genes encoding polygalacturonase II." Bussink H.J.D., Buxton F.P., Visser J. Curr. Genet. 19:467-474(1991) [PubMed: 1878999] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: NW756.
[2]	"Cloning and DNA sequence analysis of a polygalacturonase cDNA from Aspergillus niger RH5344." Ruttowski E., Labitzke R., Khanh N.Q., Loeffler F., Gottschalk M., Jany K.-D. Biochim. Biophys. Acta 1087:104-106(1990) [PubMed: 2400785] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 28-57. Strain: RH 5344.
[3]	"Characterization of a polygalacturonase gene of Aspergillus niger RH5344." Ruttkowski E., Khanh N.Q., Wientjes F.J., Gottschalk M. Mol. Microbiol. 5:1353-1361(1991) [PubMed: 1787790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RH 5344.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X58894 Genomic DNA. Translation: CAA41695.1. X52903 mRNA. Translation: CAA37085.1. X54146 Genomic DNA. Translation: CAA38085.1.
3D structure databases
HSSP	HSSP built from PDB template 1CZF based on UniProtKB P26214.
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Enzyme and pathway databases
BRENDA	3.2.1.15. 81712.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 4 hits. [Graphical view]
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR2_ASPTU

Accession

Primary (citable) accession number: P19805

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)