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Protein

Nucleoside diphosphate kinase B

Gene

Nme2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Negatively regulates Rho activity by interacting with AKAP13/LBC. Exhibits histidine protein kinase activity (By similarity).By similarity

Catalytic activityi

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12ATPBy similarity1
Binding sitei60ATPBy similarity1
Binding sitei88ATPBy similarity1
Binding sitei94ATPBy similarity1
Binding sitei105ATPBy similarity1
Binding sitei115ATPBy similarity1
Active sitei118Pros-phosphohistidine intermediateBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • drug binding Source: RGD
  • fatty acid binding Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • nucleoside diphosphate kinase activity Source: RGD
  • protein histidine kinase activity Source: UniProtKB-EC
  • protein serine/threonine kinase activity Source: RGD

GO - Biological processi

  • adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: RGD
  • cellular response to fatty acid Source: RGD
  • cellular response to glucose stimulus Source: RGD
  • cellular response to oxidative stress Source: RGD
  • CTP biosynthetic process Source: RGD
  • GTP biosynthetic process Source: RGD
  • integrin-mediated signaling pathway Source: UniProtKB
  • negative regulation of apoptotic process Source: HGNC
  • negative regulation of myeloid leukocyte differentiation Source: RGD
  • nucleoside diphosphate phosphorylation Source: RGD
  • positive regulation of epithelial cell proliferation Source: HGNC
  • positive regulation of keratinocyte differentiation Source: HGNC
  • positive regulation of neuron projection development Source: RGD
  • positive regulation of transcription by RNA polymerase II Source: UniProtKB
  • protein autophosphorylation Source: RGD
  • protein complex oligomerization Source: RGD
  • regulation of apoptotic process Source: GO_Central
  • regulation of epidermis development Source: HGNC
  • response to growth hormone Source: RGD
  • UTP biosynthetic process Source: RGD

Keywordsi

Molecular functionKinase, Transferase
Biological processNucleotide metabolism
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-499943 Interconversion of nucleotide di- and triphosphates
R-RNO-6798695 Neutrophil degranulation
SABIO-RKiP19804

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside diphosphate kinase B (EC:2.7.4.6)
Short name:
NDK B
Short name:
NDP kinase B
Alternative name(s):
Histidine protein kinase NDKB (EC:2.7.13.3)
P18
Gene namesi
Name:Nme2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi619877 Nme2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001371191 – 152Nucleoside diphosphate kinase BAdd BLAST152

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PaxDbiP19804
PRIDEiP19804

2D gel databases

World-2DPAGEi0004:P19804

PTM databases

iPTMnetiP19804
PhosphoSitePlusiP19804
SwissPalmiP19804

Expressioni

Gene expression databases

BgeeiENSRNOG00000002671
GenevisibleiP19804 RN

Interactioni

Subunit structurei

Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with AKAP13 and CAPN8. Interacts ITGB1BP1 (via C-terminal domain region) (By similarity).By similarity

Protein-protein interaction databases

BioGridi249827, 4 interactors
IntActiP19804, 1 interactor
MINTiP19804
STRINGi10116.ENSRNOP00000003611

Structurei

3D structure databases

ProteinModelPortaliP19804
SMRiP19804
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 66Interaction with AKAP13By similarityAdd BLAST66

Sequence similaritiesi

Belongs to the NDK family.Curated

Phylogenomic databases

eggNOGiKOG0888 Eukaryota
COG0105 LUCA
GeneTreeiENSGT00760000119146
HOGENOMiHOG000224564
HOVERGENiHBG000423
InParanoidiP19804
KOiK00940
OMAiELLAYMT
OrthoDBiEOG091G0PLD
PhylomeDBiP19804
TreeFamiTF106373

Family and domain databases

Gene3Di3.30.70.141, 1 hit
HAMAPiMF_00451 NDP_kinase, 1 hit
InterProiView protein in InterPro
IPR034907 NDK-like_dom
IPR036850 NDK-like_dom_sf
IPR001564 Nucleoside_diP_kinase
IPR023005 Nucleoside_diP_kinase_AS
PfamiView protein in Pfam
PF00334 NDK, 1 hit
PRINTSiPR01243 NUCDPKINASE
SMARTiView protein in SMART
SM00562 NDK, 1 hit
SUPFAMiSSF54919 SSF54919, 1 hit
PROSITEiView protein in PROSITE
PS00469 NDP_KINASES, 1 hit

Sequencei

Sequence statusi: Complete.

P19804-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ
60 70 80 90 100
HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK
110 120 130 140 150
PGTIRGDFCI QVGRNIIHGS DSVESAEKEI GLWFKPEELI DYKSCAHDWV

YE
Length:152
Mass (Da):17,283
Last modified:February 1, 1991 - v1
Checksum:i1A5C3F84C1F413EC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti89V → W in AAA42017 (PubMed:1316151).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55331 mRNA Translation: AAA41684.1
M91597 mRNA Translation: AAA42017.1
BC086599 mRNA Translation: AAH86599.1
PIRiA41849 A38369
RefSeqiNP_114021.2, NM_031833.2
UniGeneiRn.927

Genome annotation databases

EnsembliENSRNOT00000003611; ENSRNOP00000003611; ENSRNOG00000002671
GeneIDi83782
KEGGirno:83782

Similar proteinsi

Entry informationi

Entry nameiNDKB_RAT
AccessioniPrimary (citable) accession number: P19804
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: May 23, 2018
This is version 167 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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