P19802 (FMRF_LYMST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: FMRFamide neuropeptides Cleaved into the following 14 chains: FLRF-amide 1 QFYRI-amide FLRF-amide 2 PN Alternative name(s): SEEPLY FMRF-amide 1 FMRF-amide 2 FMRF-amide 3 FMRF-amide 4 FMRF-amide 5 FMRF-amide 6 FMRF-amide 7 FMRF-amide 8 FMRF-amide 9 EFLRI-amide
Organism	Lymnaea stagnalis (Great pond snail)
Taxonomic identifier	6523 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Mollusca › Gastropoda › Heterobranchia › Euthyneura › Panpulmonata › Hygrophila › Lymnaeoidea › Lymnaeidae › Lymnaea

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	FMRFamide induces contractions in visceral and somatic musculature as well as in the heart. May play a role as cotransmitters or modulators in a number of significant neuronal systems.
Subcellular location	Secreted.
Tissue specificity	Expressed in 280 cells of the CNS including the EGP heart excitatory motoneurons.
Sequence similarities	Belongs to the FARP (FMRFamide related peptide) family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal
Molecular function	Neuropeptide
PTM	Amidation Cleavage on pair of basic residues
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	neuropeptide signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Isoform 1 and isoform 2 only share the N-terminal signal sequence.

Isoform 1 (identifier: P19802-1)

Also known as: FMRFamide;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P42565-1)

Also known as: FMRFamide-related;

The sequence of this isoform can be found in the external entry P42565.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform 3 (identifier: P19802-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: MKTWSHVALLACLSIKWLTCVMADSIYCDDPDMCSM → MYSPTLIVCLSFFHSAVTKRFLRFGRALDTT

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 35	35	Potential
	Propeptide	36 – 37	2			PRO_0000009668
	Peptide	40 – 43	4	FLRF-amide 1		PRO_0000009669
	Propeptide	46 – 56	11			PRO_0000009670
	Peptide	59 – 63	5	QFYRI-amide		PRO_0000009671
	Propeptide	66 – 73	8			PRO_0000009672
	Peptide	76 – 79	4	FLRF-amide 2		PRO_0000009673
	Peptide	82 – 103	22	PN Ref.4		PRO_0000009674
	Propeptide	108 – 149	42			PRO_0000009675
	Peptide	152 – 155	4	FMRF-amide 1		PRO_0000009676
	Propeptide	158 – 163	6			PRO_0000009677
	Peptide	166 – 169	4	FMRF-amide 2		PRO_0000009678
	Propeptide	171 – 172	2			PRO_0000009679
	Peptide	173 – 176	4	FMRF-amide 3		PRO_0000009680
	Propeptide	179 – 184	6			PRO_0000009681
	Peptide	187 – 190	4	FMRF-amide 4		PRO_0000009682
	Peptide	194 – 197	4	FMRF-amide 5		PRO_0000009683
	Propeptide	200 – 204	5			PRO_0000009684
	Peptide	207 – 210	4	FMRF-amide 6		PRO_0000009685
	Propeptide	213 – 217	5			PRO_0000009686
	Peptide	220 – 223	4	FMRF-amide 7		PRO_0000009687
	Propeptide	226 – 251	26			PRO_0000009688
	Peptide	254 – 257	4	FMRF-amide 8		PRO_0000009689
	Propeptide	260 – 263	4			PRO_0000009690
	Peptide	266 – 269	4	FMRF-amide 9		PRO_0000009691
	Propeptide	272 – 278	7			PRO_0000009692
	Peptide	281 – 285	5	EFLRI-amide		PRO_0000009693
	Propeptide	289 – 306	18			PRO_0000009694
Amino acid modifications
	Modified residue	43	1	Phenylalanine amide
	Modified residue	63	1	Isoleucine amide
	Modified residue	79	1	Phenylalanine amide
	Modified residue	155	1	Phenylalanine amide
	Modified residue	169	1	Phenylalanine amide
	Modified residue	176	1	Phenylalanine amide
	Modified residue	190	1	Phenylalanine amide
	Modified residue	197	1	Phenylalanine amide
	Modified residue	210	1	Phenylalanine amide
	Modified residue	223	1	Phenylalanine amide
	Modified residue	257	1	Phenylalanine amide
	Modified residue	269	1	Phenylalanine amide
	Modified residue	285	1	Isoleucine amide
Natural variations
	Alternative sequence	1 – 36	36	MKTWS…DMCSM → MYSPTLIVCLSFFHSAVTKR FLRFGRALDTT in isoform 3.		VSP_001564
Experimental info
	Sequence conflict	91	1	L → P in AAA63280. Ref.1
	Sequence conflict	273	1	E → Q in AAA63280. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (FMRFamide) [UniParc]. Last modified November 1, 1995. Version 2. Checksum: AB2361EFF2C4EF18	FASTA	306	36,351
	10 20 30 40 50 60 MKTWSHVALL ACLSIKWLTC VMADSIYCDD PDMCSMTKRF LRFGRALDTT DPFIRLRRQF 70 80 90 100 110 120 YRIGRGGYQP YQDKRFLRFG RSEQPDVDDY LRDVVLQSEE PLYRKRRSTE AGGQSEEMTH 130 140 150 160 170 180 RTARSAPEPA AENREIMKRE TGAEDLDEEK RFMRFGRGDE EAEKRFMRFG KSFMRFGRDM 190 200 210 220 230 240 SDVDKRFMRF GKRFMRFGRE PGTDKRFMRF GREPGADKRF MRFGKSFDGE EENDDDLYYN 250 260 270 280 290 300 ESDADSNDDV DKRFMRFGKS AEEKRFMRFG KSEDASRDKK EFLRIGKRES RSAEVENNIQ IAAKQS
	Isoform 2 (FMRFamide-related) [UniParc]. See P42565.		–	–
	Isoform 3 [UniParc]. Checksum: 43038865D26D9B20	FASTA	301	35,847
	10 20 30 40 50 60 MYSPTLIVCL SFFHSAVTKR FLRFGRALDT TTKRFLRFGR ALDTTDPFIR LRRQFYRIGR 70 80 90 100 110 120 GGYQPYQDKR FLRFGRSEQP DVDDYLRDVV LQSEEPLYRK RRSTEAGGQS EEMTHRTARS 130 140 150 160 170 180 APEPAAENRE IMKRETGAED LDEEKRFMRF GRGDEEAEKR FMRFGKSFMR FGRDMSDVDK 190 200 210 220 230 240 RFMRFGKRFM RFGREPGTDK RFMRFGREPG ADKRFMRFGK SFDGEEENDD DLYYNESDAD 250 260 270 280 290 300 SNDDVDKRFM RFGKSAEEKR FMRFGKSEDA SRDKKEFLRI GKRESRSAEV ENNIQIAAKQ S

References

[1]	"Cardioactive neuropeptide Phe-Met-Arg-Phe-NH2 (FMRFamide) and novel related peptides are encoded in multiple copies by a single gene in the snail Lymnaea stagnalis." Linacre A., Kellett E., Saunders S., Bright K., Benjamin P.R., Burke J.F. J. Neurosci. 10:412-419(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Genomic organization of the FMRFamide gene in Lymnaea: multiple exons encoding novel neuropeptides." Kellett E., Saunders S.E., Li K.W., Staddon J.W., Benjamin P.R., Burke J.F. J. Neurosci. 14:6564-6570(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[3]	"Cell-specific alternative RNA splicing of an FMRFamide gene transcript in the brain." Saunders S.E., Kellett E., Bright K., Benjamin P.R., Burke J.F. J. Neurosci. 12:1033-1039(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: CNS.
[4]	"Processing of the FMRFamide precursor protein in the snail Lymnaea stagnalis: characterization and neuronal localization of a novel peptide, 'SEEPLY'." Santama N., Li K.W., Bright K.E., Yeoman M., Geraerts W.P.M., Benjamin P.R., Burke J.F. Eur. J. Neurosci. 5:1003-1016(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 82-103 (PN). Tissue: CNS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M37629 mRNA. Translation: AAA63280.1. M87479 Genomic DNA. No translation available. S38686 mRNA. Translation: AAB21767.1. S94982 Genomic DNA. Translation: AAB21764.1.
PIR	A37016. F44840.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002544. FMRFamid-related_peptide-like. [Graphical view]
Pfam	PF01581. FARP. 13 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FMRF_LYMST

Accession

Primary (citable) accession number: P19802

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)