Reviewed, UniProtKB/Swiss-Prot P19799 (TRY1_XENLA)

Names and origin

Protein names	Recommended name:     Trypsin     EC=3.4.21.4
Organism	Xenopus laevis (African clawed frog)
Taxonomic identifier	8355 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	By similarity
	Propeptide	16 – 20	5	Activation peptide		PRO_0000028233
	Chain	21 – 243	223	Trypsin		PRO_0000028234
Regions
	Domain	21 – 241	221	Peptidase S1
Sites
	Active site	60	1	Charge relay system By similarity
	Active site	104	1	Charge relay system By similarity
	Active site	197	1	Charge relay system By similarity
	Metal binding	72	1	Calcium By similarity
	Metal binding	74	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	82	1	Calcium By similarity
	Site	191	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	27 ↔ 157		By similarity
	Disulfide bond	45 ↔ 61		By similarity
	Disulfide bond	129 ↔ 230		By similarity
	Disulfide bond	136 ↔ 203		By similarity
	Disulfide bond	168 ↔ 182		By similarity
	Disulfide bond	193 ↔ 217		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P19799-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: C5B8345A8B3F8031	FASTA	243	25,492
	10 20 30 40 50 60 MKFLLLCVLL GAAAAFDDDK IIGGATCAKS SVPYIVSLNS GYHFCGGSLI TNQWVVSAAH 70 80 90 100 110 120 CYKASIQVRL GEHNIALSEG TEQFISSSKV IRHSGYNSYT LDNDIMLIKL SSPASLNAAV 130 140 150 160 170 180 NTVPLPSGCS AAGTSCLISG WGNTLSNGSN YPDLLQCLNA PILTNAQCNS AYPGEITANM 190 200 210 220 230 240 ICVGYMEGGK DSCQGDSGGP VVCNGQLQGV VSWGYGCAMR NYPGVYTKVC NYNAWIQNTI AAN

References

[1]	"Developmental and thyroid hormone-dependent regulation of pancreatic genes in Xenopus laevis." Shi Y.B., Brown D.D. Genes Dev. 4:1107-1113(1990) [PubMed: 2210372] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pancreas.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X53458 mRNA. Translation: CAA37538.1.
PIR	A35871.
UniGene	Xl.119
3D structure databases
SMR	P19799. Positions 21-243.
ModBase	Search...
Protein family/group databases
MEROPS	S01.126.
Phylogenomic databases
HOVERGEN	P19799.
Enzyme and pathway databases
BRENDA	3.4.21.4. 648.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. IPR009003. Ser/Cys_Pept_Trypsin-like. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRY1_XENLA

Accession

Primary (citable) accession number: P19799

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	November 24, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project