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Protein

3-phosphoshikimate 1-carboxyvinyltransferase

Gene

aroA

Organism
Salmonella typhi
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.UniRule annotation

Catalytic activityi

Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive (aroF), Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase (aroG), Phospho-2-dehydro-3-deoxyheptonate aldolase (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase (aroF)
  2. 3-dehydroquinate synthase (aroB), 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD), 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase 2 (aroL), Shikimate kinase 1 (aroK), Shikimate kinase 2 (aroL), Shikimate kinase 1 (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA), 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC), Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei27 – 271Shikimate-3-phosphateUniRule annotation
Binding sitei124 – 1241PhosphoenolpyruvateUniRule annotation
Binding sitei197 – 1971Shikimate-3-phosphateUniRule annotation
Active sitei313 – 3131Proton acceptorUniRule annotation
Binding sitei336 – 3361Shikimate-3-phosphateUniRule annotation
Binding sitei340 – 3401Shikimate-3-phosphateUniRule annotation
Active sitei341 – 3411Proton donorUniRule annotation
Binding sitei344 – 3441PhosphoenolpyruvateUniRule annotation
Binding sitei386 – 3861PhosphoenolpyruvateUniRule annotation
Binding sitei411 – 4111PhosphoenolpyruvateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00053; UER00089.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferaseUniRule annotation (EC:2.5.1.19UniRule annotation)
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthaseUniRule annotation
Short name:
EPSP synthaseUniRule annotation
Short name:
EPSPSUniRule annotation
Gene namesi
Name:aroAUniRule annotation
Ordered Locus Names:STY0978, t1956
OrganismiSalmonella typhi
Taxonomic identifieri90370 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000002670 Componenti: Chromosome
  • UP000000541 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4274273-phosphoshikimate 1-carboxyvinyltransferasePRO_0000088285Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi220341.STY0978.

Structurei

3D structure databases

ProteinModelPortaliP19786.
SMRiP19786. Positions 1-427.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 232Shikimate-3-phosphate bindingUniRule annotation
Regioni94 – 974PhosphoenolpyruvateUniRule annotation
Regioni169 – 1713Shikimate-3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the EPSP synthase family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105CMY. Bacteria.
COG0128. LUCA.
HOGENOMiHOG000247372.
KOiK00800.
OMAiIGRNSVQ.
OrthoDBiEOG6Z6FZ4.

Family and domain databases

Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00210. EPSP_synth.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR006264. EPSP_synthase.
IPR023193. EPSP_synthase_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000505. EPSPS. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01356. aroA. 1 hit.
PROSITEiPS00104. EPSP_SYNTHASE_1. 1 hit.
PS00885. EPSP_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19786-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESLTLQPIA RVDGAINLPG SKSVSNRALL LAALACGKTV LTNLLDSDDV
60 70 80 90 100
RHMLNALSAL GINYTLSADR TRCDITGNGG PLRASGTLEL FLGNAGTAMR
110 120 130 140 150
PLAAALCLGQ NEIVLTGEPR MKERPIGHLV DSLRQGGANI DYLEQENYPP
160 170 180 190 200
LRLRGGFIGG DIEVDGSVSS QFLTALLMTA PLAPEDTIIR VKGELVSKPY
210 220 230 240 250
IDITLNLMKT FGVEIANHHY QQFVVKGGQQ YHSPGRYLVE GDASSASYFL
260 270 280 290 300
AAGAIKGGTV KVTGIGRKSM QGDIRFADVL EKMGATITWG DDFIACTRGE
310 320 330 340 350
LHAIDMDMNH IPDAAMTIAT TALFAKGTTT LRNIYNWRVK ETDRLFAMAT
360 370 380 390 400
ELRKVGAEVE EGHDYIRITP PAKLQHADIG TYNDHRMAMC FSLVALSDTP
410 420
VTILDPKCTA KTFPDYFEQL ARMSTPA
Length:427
Mass (Da):46,219
Last modified:December 5, 2001 - v2
Checksum:i99F0A7096303B30A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti254 – 2541A → G in CAA38417 (PubMed:2235501).Curated
Sequence conflicti267 – 2671R → G in CAA38417 (PubMed:2235501).Curated
Sequence conflicti281 – 2811E → H in CAA38417 (PubMed:2235501).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54545 Genomic DNA. Translation: CAA38417.1.
AL513382 Genomic DNA. Translation: CAD05378.1.
AE014613 Genomic DNA. Translation: AAO69570.1.
PIRiS12096.
RefSeqiNP_455465.1. NC_003198.1.
WP_000445205.1. NZ_FFOG01000007.1.

Genome annotation databases

EnsemblBacteriaiAAO69570; AAO69570; t1956.
CAD05378; CAD05378; CAD05378.
GeneIDi1247410.
KEGGistt:t1956.
sty:STY0978.
PATRICi18539837. VBISalEnt120419_0987.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54545 Genomic DNA. Translation: CAA38417.1.
AL513382 Genomic DNA. Translation: CAD05378.1.
AE014613 Genomic DNA. Translation: AAO69570.1.
PIRiS12096.
RefSeqiNP_455465.1. NC_003198.1.
WP_000445205.1. NZ_FFOG01000007.1.

3D structure databases

ProteinModelPortaliP19786.
SMRiP19786. Positions 1-427.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi220341.STY0978.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO69570; AAO69570; t1956.
CAD05378; CAD05378; CAD05378.
GeneIDi1247410.
KEGGistt:t1956.
sty:STY0978.
PATRICi18539837. VBISalEnt120419_0987.

Phylogenomic databases

eggNOGiENOG4105CMY. Bacteria.
COG0128. LUCA.
HOGENOMiHOG000247372.
KOiK00800.
OMAiIGRNSVQ.
OrthoDBiEOG6Z6FZ4.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00089.

Family and domain databases

Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00210. EPSP_synth.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR006264. EPSP_synthase.
IPR023193. EPSP_synthase_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000505. EPSPS. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01356. aroA. 1 hit.
PROSITEiPS00104. EPSP_SYNTHASE_1. 1 hit.
PS00885. EPSP_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of the aroA gene from Salmonella typhi encoding 5-enolpyruvylshikimate 3-phosphate synthase."
    Chatfield S., Dougan G., Charles I.G.
    Nucleic Acids Res. 18:6133-6133(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 700931 / Ty2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CT18.
  3. "Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18."
    Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R.
    J. Bacteriol. 185:2330-2337(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700931 / Ty2.

Entry informationi

Entry nameiAROA_SALTI
AccessioniPrimary (citable) accession number: P19786
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: December 5, 2001
Last modified: May 11, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.