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Protein

3-phosphoshikimate 1-carboxyvinyltransferase

Gene

aroA

Organism
Salmonella typhi
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.UniRule annotation

Catalytic activityi

Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive (aroF), Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase (aroG)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase 2 (aroL), Shikimate kinase 1 (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei27Shikimate-3-phosphateUniRule annotation1
Binding sitei124PhosphoenolpyruvateUniRule annotation1
Binding sitei197Shikimate-3-phosphateUniRule annotation1
Active sitei313Proton acceptorUniRule annotation1
Binding sitei336Shikimate-3-phosphateUniRule annotation1
Binding sitei340Shikimate-3-phosphateUniRule annotation1
Active sitei341Proton donorUniRule annotation1
Binding sitei344PhosphoenolpyruvateUniRule annotation1
Binding sitei386PhosphoenolpyruvateUniRule annotation1
Binding sitei411PhosphoenolpyruvateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00053; UER00089

Names & Taxonomyi

Protein namesi
Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferaseUniRule annotation (EC:2.5.1.19UniRule annotation)
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthaseUniRule annotation
Short name:
EPSP synthaseUniRule annotation
Short name:
EPSPSUniRule annotation
Gene namesi
Name:aroAUniRule annotation
Ordered Locus Names:STY0978, t1956
OrganismiSalmonella typhi
Taxonomic identifieri90370 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000002670 Componenti: Chromosome
  • UP000000541 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000882851 – 4273-phosphoshikimate 1-carboxyvinyltransferaseAdd BLAST427

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi220341.STY0978

Structurei

3D structure databases

ProteinModelPortaliP19786
SMRiP19786
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 23Shikimate-3-phosphate bindingUniRule annotation2
Regioni94 – 97PhosphoenolpyruvateUniRule annotation4
Regioni169 – 171Shikimate-3-phosphate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the EPSP synthase family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105CMY Bacteria
COG0128 LUCA
HOGENOMiHOG000247372
KOiK00800
OMAiPSSAFFF

Family and domain databases

Gene3Di3.65.10.10, 2 hits
HAMAPiMF_00210 EPSP_synth, 1 hit
InterProiView protein in InterPro
IPR001986 Enolpyruvate_Tfrase_dom
IPR036968 Enolpyruvate_Tfrase_sf
IPR006264 EPSP_synthase
IPR023193 EPSP_synthase_CS
IPR013792 RNA3'P_cycl/enolpyr_Trfase_a/b
PfamiView protein in Pfam
PF00275 EPSP_synthase, 1 hit
PIRSFiPIRSF000505 EPSPS, 1 hit
SUPFAMiSSF55205 SSF55205, 1 hit
TIGRFAMsiTIGR01356 aroA, 1 hit
PROSITEiView protein in PROSITE
PS00104 EPSP_SYNTHASE_1, 1 hit
PS00885 EPSP_SYNTHASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P19786-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESLTLQPIA RVDGAINLPG SKSVSNRALL LAALACGKTV LTNLLDSDDV
60 70 80 90 100
RHMLNALSAL GINYTLSADR TRCDITGNGG PLRASGTLEL FLGNAGTAMR
110 120 130 140 150
PLAAALCLGQ NEIVLTGEPR MKERPIGHLV DSLRQGGANI DYLEQENYPP
160 170 180 190 200
LRLRGGFIGG DIEVDGSVSS QFLTALLMTA PLAPEDTIIR VKGELVSKPY
210 220 230 240 250
IDITLNLMKT FGVEIANHHY QQFVVKGGQQ YHSPGRYLVE GDASSASYFL
260 270 280 290 300
AAGAIKGGTV KVTGIGRKSM QGDIRFADVL EKMGATITWG DDFIACTRGE
310 320 330 340 350
LHAIDMDMNH IPDAAMTIAT TALFAKGTTT LRNIYNWRVK ETDRLFAMAT
360 370 380 390 400
ELRKVGAEVE EGHDYIRITP PAKLQHADIG TYNDHRMAMC FSLVALSDTP
410 420
VTILDPKCTA KTFPDYFEQL ARMSTPA
Length:427
Mass (Da):46,219
Last modified:December 5, 2001 - v2
Checksum:i99F0A7096303B30A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti254A → G in CAA38417 (PubMed:2235501).Curated1
Sequence conflicti267R → G in CAA38417 (PubMed:2235501).Curated1
Sequence conflicti281E → H in CAA38417 (PubMed:2235501).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54545 Genomic DNA Translation: CAA38417.1
AL513382 Genomic DNA Translation: CAD05378.1
AE014613 Genomic DNA Translation: AAO69570.1
PIRiS12096
RefSeqiNP_455465.1, NC_003198.1
WP_000445205.1, NZ_PHKG01000006.1

Genome annotation databases

EnsemblBacteriaiAAO69570; AAO69570; t1956
CAD05378; CAD05378; CAD05378
GeneIDi1247410
KEGGistt:t1956
sty:STY0978
PATRICifig|220341.7.peg.987

Similar proteinsi

Entry informationi

Entry nameiAROA_SALTI
AccessioniPrimary (citable) accession number: P19786
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: December 5, 2001
Last modified: March 28, 2018
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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