Reviewed, UniProtKB/Swiss-Prot P19762 (HN_MUMP1)

Names and origin

Protein names

Recommended name:
    Hemagglutinin-neuraminidase
    EC=3.2.1.18

Gene names

Name:

HN

Organism

Mumps virus (strain SBL-1) (MuV)

Taxonomic identifier

11173 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Rubulavirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	582 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion By similarity. Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins By similarity.
Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Subcellular location	Virion membrane; Single-pass type II membrane protein Potential. Host cell membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the paramyxoviruses hemagglutinin-neuraminidase family.

Ontologies

Keywords
Cellular component	Envelope protein Host cell membrane Host membrane Membrane Virion
Domain	Signal-anchor Transmembrane
Molecular function	Hemagglutinin Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	viral infectious cycle Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell viral envelope Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	exo-alpha-sialidase activity Inferred from electronic annotation. Source: EC host cell surface receptor binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 582	582	Hemagglutinin-neuraminidase		PRO_0000142605
Regions
	Topological domain	1 – 34	34	Cytoplasmic Potential
	Transmembrane	35 – 55	21	Membrane anchor Potential
	Topological domain	56 – 582	527	Extracellular Potential
Amino acid modifications
	Glycosylation	284	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	329	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	400	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	448	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	507	1	N-linked (GlcNAc...); by host Potential

Sequences

	Sequence		Length	Mass (Da)
	P19762-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 18F95300AA33DC7F	FASTA	582	63,885
	10 20 30 40 50 60 MEPSKLFIMS DNATVAPGPV VNAAGKKTFR TCFRILVLSV QAVTLILVIV TLGELIRMIN 70 80 90 100 110 120 DQGLSNQLSS ITDKIRESAA VIASAVGVMN QVIHGVTVSL PLQIEGNQNQ LLSTLATICT 130 140 150 160 170 180 NRNQVSNCST NIPLVNDLRF INGINKFIIE DYATHDFSIG NPLNMPSFIP TATSPNGCTR 190 200 210 220 230 240 IPSFSLGKTH WCYTHNVINA NCKDHTSSNQ YVSMGILVQT ASGYPMFKTL KIQYLSDGLN 250 260 270 280 290 300 RKSCSIATVP DGCAMYCYVS TQLEANDYAG SSPPTQKLTL LFYNDTITER TISPSGLEGN 310 320 330 340 350 360 WATLVPGVGS GIYFENKLIF PAYGGVLPNS TLGVKSAREF FRPVNPYNPC SGPPQELDQR 370 380 390 400 410 420 ALRSYFPRYF SSRRVQSAFL VCAWNQILVT NCELVVPSNN QTLMGAEGRV LLINNRLLYY 430 440 450 460 470 480 QRSTSWWPYE LLYEISFTFT NSGQSSVNMS WIPIYSFTPP GSGNCSGKNV CPTVCVSGVY 490 500 510 520 530 540 LDPWPLTPYS HQSGINRNFY FTGALLNSST TRVNPTLYVS ALNNLKVLAP YGTQGLFASY 550 560 570 580 TTTTCFQDTG DASVYCVYIM ELASNIVGEF QILPVLARLT IT

References

[1]	"Complete nucleotide sequence of the hemagglutinin-neuraminidase (HN) mRNA of mumps virus and comparison of paramyxovirus HN proteins." Koevamees J., Norrby E., Elango N. Virus Res. 12:87-96(1989) [PubMed: 2718627] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Strain-variable editing during transcription of the P gene of mumps virus may lead to the generation of non-structural proteins NS1 (V) and NS2." Elliott G.D., Yeo R.P., Afzal M.A., Simpson E.J.B., Curran J.A., Rima B.K. J. Gen. Virol. 71:1555-1560(1990) [PubMed: 2165137] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-153.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M55065 Genomic RNA. Translation: AAA74751.1. D00663 Genomic RNA. Translation: BAA00563.1.
PIR	HNNZSB. A42758.
3D structure databases
SMR	P19762. Positions 135-582.
ModBase	Search...
Protein family/group databases
CAZy	GH83. Glycoside Hydrolase Family 83.
Family and domain databases
InterPro	IPR000665. Hemagglutn-neuramid_glycoprot. IPR016285. Hemagglutn-neuramid_paramyxo. IPR011040. Neuraminidase. [Graphical view]
Pfam	PF00423. HN. 1 hit. [Graphical view]
PIRSF	PIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HN_MUMP1

Accession

Primary (citable) accession number: P19762
Secondary accession number(s): P33480

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	January 19, 2010
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)