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Protein

Hemagglutinin-neuraminidase

Gene

HN

Organism
Sendai virus (strain Fushimi) (SeV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion (By similarity).By similarity
Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins.By similarity

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

GO - Molecular functioni

  1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
  2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
  3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC

GO - Biological processi

  1. viral entry into host cell Source: UniProtKB-KW
  2. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Keywords - Biological processi

Host-virus interaction, Viral attachment to host cell, Virus entry into host cell

Protein family/group databases

CAZyiGH83. Glycoside Hydrolase Family 83.

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-neuraminidase (EC:3.2.1.18)
Short name:
HN protein
Gene namesi
Name:HN
OrganismiSendai virus (strain Fushimi) (SeV)
Taxonomic identifieri11195 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesParamyxoviridaeParamyxovirinaeRespirovirus
Virus hostiCavia cutleri (Guinea pig) [TaxID: 10144]
Cricetidae sp. (Hamster) [TaxID: 36483]
Mus musculus (Mouse) [TaxID: 10090]
Rattus norvegicus (Rat) [TaxID: 10116]
ProteomesiUP000006825 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3737IntravirionBy similarityAdd
BLAST
Transmembranei38 – 5821HelicalBy similarityAdd
BLAST
Topological domaini59 – 575517Virion surfaceBy similarityAdd
BLAST

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 575575Hemagglutinin-neuraminidasePRO_0000142637Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi77 – 771N-linked (GlcNAc...); by hostBy similarity
Disulfide bondi129 – 129InterchainSequence Analysis
Glycosylationi499 – 4991N-linked (GlcNAc...); by hostBy similarity
Glycosylationi511 – 5111N-linked (GlcNAc...); by hostBy similarity

Post-translational modificationi

N-glycosylated; glycans consist of a mixture of high mannose-type oligosaccharides and of complex-type oligosaccharides.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homotetramer; composed of disulfide-linked homodimers. Interacts with F protein trimer (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP19758.
SMRiP19758. Positions 144-572.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni10 – 145Incorporation in virionBy similarity
Regioni59 – 14082Involved in interaction with F proteinBy similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR000665. Hemagglutn-neuramid.
IPR016285. Hemagglutn-neuramid_paramyxo.
IPR011040. Sialidases.
[Graphical view]
PfamiPF00423. HN. 1 hit.
[Graphical view]
PIRSFiPIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
SUPFAMiSSF50939. SSF50939. 1 hit.

Sequencei

Sequence statusi: Complete.

P19758-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGDRGKRDS YWSTSPSGST TKLASGWERS SKVDTWLLIL SFTQWALSIA
60 70 80 90 100
TVIICIIISA RQGYSMKEYS MTVEALNMSS REVKESLTSL IRQEVIARAV
110 120 130 140 150
NIQSSVQTGI PVLLNKNSRD VIQMIDKSCS RQELTQLCES TIAVHHAEGI
160 170 180 190 200
APLEPHSFWR CPVGEPYLSS DPKISLLPGP SLLSGSTTIS GCVRLPSLSI
210 220 230 240 250
GEAIYAYSSN LITQGCADIG KSYQVLQLGY ISLNSDMFPD LNPVVSHTYD
260 270 280 290 300
INDNRKSCSV VATGTRGYQL CSMPTVDERT DYSSDGIEDL VLDVLDLKGS
310 320 330 340 350
TKSHRYRNSE VDLDHPFSAL YPSVGNGIAT EGSLIFLGYG GLTTPLQGDT
360 370 380 390 400
KCRTQGCQQV SQDTCNEALK ITWLGGKQVV SVIIQVNDYL SERPKIRVTT
410 420 430 440 450
IPITQNYLGA EGRLLKLGDR VYIYTRSSGW HSQLQIGVLD VSHPLTINWT
460 470 480 490 500
PHEALSRPGN EECNWYNTCP KECISGVYTD AYPLSPDAAN VATVTLYANT
510 520 530 540 550
SRVNPTIMYS NTTNIINMLR IKDVQLEAAY TTTSCITHFG KGYCFHIIEI
560 570
NQKSLNTLQP MLFKTSIPKL CKAES
Length:575
Mass (Da):63,348
Last modified:February 1, 1991 - v1
Checksum:i93FD0532F6147BF6
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti86 – 861S → T.
Natural varianti525 – 5251Q → K.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56131 Genomic RNA. Translation: CAA39596.1.
U06433 Genomic RNA. Translation: AAC54272.1.
PIRiS12135.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56131 Genomic RNA. Translation: CAA39596.1.
U06433 Genomic RNA. Translation: AAC54272.1.
PIRiS12135.

3D structure databases

ProteinModelPortaliP19758.
SMRiP19758. Positions 144-572.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH83. Glycoside Hydrolase Family 83.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR000665. Hemagglutn-neuramid.
IPR016285. Hemagglutn-neuramid_paramyxo.
IPR011040. Sialidases.
[Graphical view]
PfamiPF00423. HN. 1 hit.
[Graphical view]
PIRSFiPIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
SUPFAMiSSF50939. SSF50939. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequencing of the HN gene of Sendai virus (strain Fushimi)."
    Neubert W.J., Willenbrink W.
    Nucleic Acids Res. 18:6427-6427(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Role of basic residues in the proteolytic activation of Sendai virus fusion glycoprotein."
    Heminaway B.R., Yang Y., Tanaka Y., Panin M., Huang Y.T., Galinski M.S.
    Virus Res. 36:15-35(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Kinetics of interactions of sendai virus envelope glycoproteins, F and HN, with endoplasmic reticulum-resident molecular chaperones, BiP, calnexin, and calreticulin."
    Tomita Y., Yamashita T., Sato H., Taira H.
    J. Biochem. 126:1090-1100(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CHAPERONES.

Entry informationi

Entry nameiHN_SENDF
AccessioniPrimary (citable) accession number: P19758
Secondary accession number(s): Q88413
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: January 7, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.