ID   PRP6_YEAST              Reviewed;         899 AA.
AC   P19735; D6VQ54;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   27-MAR-2024, entry version 193.
DE   RecName: Full=Pre-mRNA-splicing factor 6;
GN   Name=PRP6; Synonyms=RNA6; OrderedLocusNames=YBR055C; ORFNames=YBR0508;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=S288c / GRF88;
RX   PubMed=2118103; DOI=10.1002/j.1460-2075.1990.tb07465.x;
RA   Legrain P., Choulika A.;
RT   "The molecular characterization of PRP6 and PRP9 yeast genes reveals a new
RT   cysteine/histidine motif common to several splicing factors.";
RL   EMBO J. 9:2775-2781(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7597852; DOI=10.1002/yea.320110511;
RA   Aljinovic G., Pohl T.M.;
RT   "Sequence and analysis of 24 kb on chromosome II of Saccharomyces
RT   cerevisiae.";
RL   Yeast 11:475-479(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA   Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA   Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA   Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA   Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA   Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA   Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA   Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA   Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA   Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA   Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA   Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA   Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA   Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA   Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA   Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA   Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA   Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA   Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA   Mewes H.-W., Kleine K.;
RT   "Complete DNA sequence of yeast chromosome II.";
RL   EMBO J. 13:5795-5809(1994).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [5]
RP   SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=10449419; DOI=10.1093/emboj/18.16.4535;
RA   Gottschalk A., Neubauer G., Banroques J., Mann M., Luehrmann R.,
RA   Fabrizio P.;
RT   "Identification by mass spectrometry and functional analysis of novel
RT   proteins of the yeast [U4/U6.U5] tri-snRNP.";
RL   EMBO J. 18:4535-4548(1999).
RN   [6]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [7]
RP   SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, AND ELECTRON
RP   MICROSCOPY.
RX   PubMed=18953335; DOI=10.1038/nsmb.1506;
RA   Hacker I., Sander B., Golas M.M., Wolf E., Karagoz E., Kastner B.,
RA   Stark H., Fabrizio P., Luhrmann R.;
RT   "Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-
RT   snRNP by electron microscopy.";
RL   Nat. Struct. Mol. Biol. 15:1206-1212(2008).
CC   -!- FUNCTION: Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-
CC       snRNP complex, one of the building blocks of the spliceosome.
CC   -!- SUBUNIT: Component of the U4/U6-U5 tri-snRNP complex composed of the
CC       U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31,
CC       PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3,
CC       SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1.
CC       {ECO:0000269|PubMed:10449419, ECO:0000269|PubMed:18953335}.
CC   -!- INTERACTION:
CC       P19735; Q06819: DIB1; NbExp=3; IntAct=EBI-227, EBI-166;
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- MISCELLANEOUS: Present with 2250 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; X53465; CAA37559.1; -; Genomic_DNA.
DR   EMBL; Z35924; CAA84998.1; -; Genomic_DNA.
DR   EMBL; Z46260; CAA86398.1; -; Genomic_DNA.
DR   EMBL; BK006936; DAA07174.1; -; Genomic_DNA.
DR   PIR; S12319; S12319.
DR   RefSeq; NP_009611.1; NM_001178403.1.
DR   PDB; 3JCM; EM; 3.80 A; G=1-899.
DR   PDB; 5GAN; EM; 3.60 A; J=1-899.
DR   PDB; 5GAP; EM; 3.60 A; J=1-899.
DR   PDB; 5NRL; EM; 7.20 A; J=1-899.
DR   PDB; 5ZWM; EM; 3.40 A; N=1-899.
DR   PDB; 5ZWO; EM; 3.90 A; N=1-899.
DR   PDBsum; 3JCM; -.
DR   PDBsum; 5GAN; -.
DR   PDBsum; 5GAP; -.
DR   PDBsum; 5NRL; -.
DR   PDBsum; 5ZWM; -.
DR   PDBsum; 5ZWO; -.
DR   AlphaFoldDB; P19735; -.
DR   EMDB; EMD-3683; -.
DR   EMDB; EMD-6972; -.
DR   EMDB; EMD-6974; -.
DR   EMDB; EMD-8012; -.
DR   EMDB; EMD-8014; -.
DR   SMR; P19735; -.
DR   BioGRID; 32756; 393.
DR   ComplexPortal; CPX-25; U4/U6.U5 tri-small nuclear ribonucleoprotein complex.
DR   ComplexPortal; CPX-29; U5 small nuclear ribonucleoprotein complex.
DR   DIP; DIP-1290N; -.
DR   IntAct; P19735; 111.
DR   MINT; P19735; -.
DR   STRING; 4932.YBR055C; -.
DR   MaxQB; P19735; -.
DR   PaxDb; 4932-YBR055C; -.
DR   PeptideAtlas; P19735; -.
DR   EnsemblFungi; YBR055C_mRNA; YBR055C; YBR055C.
DR   GeneID; 852344; -.
DR   KEGG; sce:YBR055C; -.
DR   AGR; SGD:S000000259; -.
DR   SGD; S000000259; PRP6.
DR   VEuPathDB; FungiDB:YBR055C; -.
DR   eggNOG; KOG0495; Eukaryota.
DR   GeneTree; ENSGT00940000172817; -.
DR   HOGENOM; CLU_007010_0_0_1; -.
DR   InParanoid; P19735; -.
DR   OMA; DGWAWYY; -.
DR   OrthoDB; 655233at2759; -.
DR   BioCyc; YEAST:G3O-29026-MONOMER; -.
DR   BioGRID-ORCS; 852344; 4 hits in 10 CRISPR screens.
DR   PRO; PR:P19735; -.
DR   Proteomes; UP000002311; Chromosome II.
DR   RNAct; P19735; Protein.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; NAS:ComplexPortal.
DR   GO; GO:0005681; C:spliceosomal complex; NAS:ComplexPortal.
DR   GO; GO:0071001; C:U4/U6 snRNP; IMP:GO_Central.
DR   GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; IDA:SGD.
DR   GO; GO:0005682; C:U5 snRNP; NAS:ComplexPortal.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IMP:GO_Central.
DR   GO; GO:0000244; P:spliceosomal tri-snRNP complex assembly; IBA:GO_Central.
DR   Gene3D; 1.25.40.10; Tetratricopeptide repeat domain; 3.
DR   InterPro; IPR003107; HAT.
DR   InterPro; IPR010491; PRP1_N.
DR   InterPro; IPR045075; Syf1-like.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   InterPro; IPR019734; TPR_repeat.
DR   PANTHER; PTHR11246; PRE-MRNA SPLICING FACTOR; 1.
DR   PANTHER; PTHR11246:SF1; PRE-MRNA-PROCESSING FACTOR 6; 1.
DR   Pfam; PF06424; PRP1_N; 1.
DR   SMART; SM00386; HAT; 7.
DR   SMART; SM00028; TPR; 3.
DR   SUPFAM; SSF48452; TPR-like; 3.
PE   1: Evidence at protein level;
KW   3D-structure; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW   Repeat; Spliceosome.
FT   CHAIN           1..899
FT                   /note="Pre-mRNA-splicing factor 6"
FT                   /id="PRO_0000205756"
FT   REPEAT          221..253
FT                   /note="HAT 1"
FT   REPEAT          255..287
FT                   /note="HAT 2"
FT   REPEAT          289..318
FT                   /note="HAT 3"
FT   REPEAT          319..350
FT                   /note="HAT 4"
FT   REPEAT          352..381
FT                   /note="HAT 5"
FT   REPEAT          383..414
FT                   /note="HAT 6"
FT   REPEAT          493..525
FT                   /note="HAT 7"
FT   REPEAT          545..578
FT                   /note="HAT 8"
FT   REPEAT          608..645
FT                   /note="HAT 9"
FT   REPEAT          648..680
FT                   /note="HAT 10"
FT   REPEAT          682..714
FT                   /note="HAT 11"
FT   REPEAT          751..783
FT                   /note="HAT 12"
FT   REPEAT          819..851
FT                   /note="HAT 13"
FT   REGION          1..65
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        32..65
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   899 AA;  104229 MW;  3E87F8AF63EAAC41 CRC64;
     MERPSFLDQE PPAGYVPGIG RGATGFSTKE KQVVSNDDKG RRIPKRYREN LNNHLQSQPK
     DDEDDEAANV FKTLELKLAQ KKKKRANEKD DDNSVDSSNV KRQFADLKES LAAVTESEWM
     DIPDATDFTR RNKRNRIQEQ LNRKTYAAPD SLIPGNVDLN KLTEEREKLL QSQIDENLAQ
     LTKNASNPIQ VNKPNAATDA LSYLKDLEND RVNSLSDATL EDLQKMRTIL KSYRKADPTN
     PQGWIASARL EEKARKFSVA KKIIENGCQE CPRSSDIWLE NIRLHESDVH YCKTLVATAI
     NFNPTSPLLW FKAIDLESTT VNKYRVVRKA LQEIPRDEGL WKLAVSFEAD KAQVIKMLEK
     ATQFIPQSMD LLTAYTNLQS YHNAKMTLNS FRKILPQEPE IWIISTLLEE RNNPDIPVDK
     LVSLLKEGLL ELSKNGYKAT LSAWLKRAEA LNDAPNSNLT CQAIVYAILE WLRESGEYES
     ELNNVDQILE KMPHSKVQIA VLKKLIQWDP CDTVLWSRLK MATESYHKIE ELLAFFQELL
     FQTKNSDDIR ANMREKSPGL LMMYVSEYWK AQKGDTRQTL VLIDQIIDFA PHNLDLRFFK
     IKLLGRSLQL DELRDFFQQT FSSLEDFKIS GTERLYYKYV NFLRYQDLNE EAIKFLNERC
     LKSFPICHKF FLQLGQIYHS MGNIEMSRET YLSGTRLVPN CPLLWVSLSK IDEIDLKNPV
     RARSILDRGL LKNPDDVLFY IAKIQMEIRL GNLDQAELLV TQALQKFPSN ALLWVEQIKL
     FKHGNKSSLK KTIFQDALRR TQNDHRVLLE IGVSFYAEAQ YETSLKWLER ALKKCSRYGD
     TWVWLFRTYA RLGKDTVDLY NMFDQCEPTY GPEWIAASKN VKMQYCTPRE ILLRLMNDK
//