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P19735 (PRP6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pre-mRNA-splicing factor 6
Gene names
Name:PRP6
Synonyms:RNA6
Ordered Locus Names:YBR055C
ORF Names:YBR0508
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length899 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.

Subunit structure

Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1. Ref.5 Ref.7

Subcellular location

Nucleus.

Miscellaneous

Present with 2250 molecules/cell in log phase SD medium.

Sequence similarities

Contains 13 HAT repeats.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

DIB1Q068193EBI-227,EBI-166

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 899899Pre-mRNA-splicing factor 6
PRO_0000205756

Regions

Repeat221 – 25333HAT 1
Repeat255 – 28733HAT 2
Repeat289 – 31830HAT 3
Repeat319 – 35032HAT 4
Repeat352 – 38130HAT 5
Repeat383 – 41432HAT 6
Repeat493 – 52533HAT 7
Repeat545 – 57834HAT 8
Repeat608 – 64538HAT 9
Repeat648 – 68033HAT 10
Repeat682 – 71433HAT 11
Repeat751 – 78333HAT 12
Repeat819 – 85133HAT 13

Sequences

Sequence LengthMass (Da)Tools
P19735 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 3E87F8AF63EAAC41

FASTA899104,229
        10         20         30         40         50         60 
MERPSFLDQE PPAGYVPGIG RGATGFSTKE KQVVSNDDKG RRIPKRYREN LNNHLQSQPK 

        70         80         90        100        110        120 
DDEDDEAANV FKTLELKLAQ KKKKRANEKD DDNSVDSSNV KRQFADLKES LAAVTESEWM 

       130        140        150        160        170        180 
DIPDATDFTR RNKRNRIQEQ LNRKTYAAPD SLIPGNVDLN KLTEEREKLL QSQIDENLAQ 

       190        200        210        220        230        240 
LTKNASNPIQ VNKPNAATDA LSYLKDLEND RVNSLSDATL EDLQKMRTIL KSYRKADPTN 

       250        260        270        280        290        300 
PQGWIASARL EEKARKFSVA KKIIENGCQE CPRSSDIWLE NIRLHESDVH YCKTLVATAI 

       310        320        330        340        350        360 
NFNPTSPLLW FKAIDLESTT VNKYRVVRKA LQEIPRDEGL WKLAVSFEAD KAQVIKMLEK 

       370        380        390        400        410        420 
ATQFIPQSMD LLTAYTNLQS YHNAKMTLNS FRKILPQEPE IWIISTLLEE RNNPDIPVDK 

       430        440        450        460        470        480 
LVSLLKEGLL ELSKNGYKAT LSAWLKRAEA LNDAPNSNLT CQAIVYAILE WLRESGEYES 

       490        500        510        520        530        540 
ELNNVDQILE KMPHSKVQIA VLKKLIQWDP CDTVLWSRLK MATESYHKIE ELLAFFQELL 

       550        560        570        580        590        600 
FQTKNSDDIR ANMREKSPGL LMMYVSEYWK AQKGDTRQTL VLIDQIIDFA PHNLDLRFFK 

       610        620        630        640        650        660 
IKLLGRSLQL DELRDFFQQT FSSLEDFKIS GTERLYYKYV NFLRYQDLNE EAIKFLNERC 

       670        680        690        700        710        720 
LKSFPICHKF FLQLGQIYHS MGNIEMSRET YLSGTRLVPN CPLLWVSLSK IDEIDLKNPV 

       730        740        750        760        770        780 
RARSILDRGL LKNPDDVLFY IAKIQMEIRL GNLDQAELLV TQALQKFPSN ALLWVEQIKL 

       790        800        810        820        830        840 
FKHGNKSSLK KTIFQDALRR TQNDHRVLLE IGVSFYAEAQ YETSLKWLER ALKKCSRYGD 

       850        860        870        880        890 
TWVWLFRTYA RLGKDTVDLY NMFDQCEPTY GPEWIAASKN VKMQYCTPRE ILLRLMNDK 

« Hide

References

« Hide 'large scale' references
[1]"The molecular characterization of PRP6 and PRP9 yeast genes reveals a new cysteine/histidine motif common to several splicing factors."
Legrain P., Choulika A.
EMBO J. 9:2775-2781(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / GRF88.
[2]"Sequence and analysis of 24 kb on chromosome II of Saccharomyces cerevisiae."
Aljinovic G., Pohl T.M.
Yeast 11:475-479(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP."
Gottschalk A., Neubauer G., Banroques J., Mann M., Luehrmann R., Fabrizio P.
EMBO J. 18:4535-4548(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy."
Hacker I., Sander B., Golas M.M., Wolf E., Karagoz E., Kastner B., Stark H., Fabrizio P., Luhrmann R.
Nat. Struct. Mol. Biol. 15:1206-1212(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, ELECTRON MICROSCOPY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X53465 Genomic DNA. Translation: CAA37559.1.
Z35924 Genomic DNA. Translation: CAA84998.1.
Z46260 Genomic DNA. Translation: CAA86398.1.
BK006936 Genomic DNA. Translation: DAA07174.1.
PIRS12319.
RefSeqNP_009611.1. NM_001178403.1.

3D structure databases

ProteinModelPortalP19735.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32756. 130 interactions.
DIPDIP-1290N.
IntActP19735. 24 interactions.
MINTMINT-392841.
STRING4932.YBR055C.

Proteomic databases

MaxQBP19735.
PaxDbP19735.
PeptideAtlasP19735.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR055C; YBR055C; YBR055C.
GeneID852344.
KEGGsce:YBR055C.

Organism-specific databases

CYGDYBR055c.
SGDS000000259. PRP6.

Phylogenomic databases

eggNOGCOG0457.
GeneTreeENSGT00550000075016.
HOGENOMHOG000246836.
KOK12855.
OMARQRFYAV.
OrthoDBEOG7GXPM5.

Enzyme and pathway databases

BioCycYEAST:G3O-29026-MONOMER.

Gene expression databases

GenevestigatorP19735.

Family and domain databases

Gene3D1.25.40.10. 2 hits.
InterProIPR003107. HAT.
IPR010491. PRP1_N.
IPR027108. Prp6/Prp1.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERPTHR11246:SF1. PTHR11246:SF1. 1 hit.
PfamPF06424. PRP1_N. 1 hit.
[Graphical view]
SMARTSM00386. HAT. 7 hits.
SM00028. TPR. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio971082.
PROP19735.

Entry information

Entry namePRP6_YEAST
AccessionPrimary (citable) accession number: P19735
Secondary accession number(s): D6VQ54
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: June 11, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families