P19735 (PRP6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre-mRNA-splicing factor 6

Gene names

Name:	PRP6
Synonyms:	RNA6
Ordered Locus Names:	YBR055C
ORF Names:	YBR0508

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	899 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in pre-mRNA splicing.
Subcellular location	Nucleus.
Miscellaneous	Present with 2250 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Contains 13 HAT repeats.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus
Domain	Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nuclear mRNA splicing, via spliceosome Inferred from physical interaction. Source: SGD
Cellular component	U4/U6 x U5 tri-snRNP complex Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DIB1	Q06819	3	EBI-227,EBI-166

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 899	899	Pre-mRNA-splicing factor 6		PRO_0000205756
Regions
	Repeat	221 – 253	33	HAT 1
	Repeat	255 – 287	33	HAT 2
	Repeat	289 – 318	30	HAT 3
	Repeat	319 – 350	32	HAT 4
	Repeat	352 – 381	30	HAT 5
	Repeat	383 – 414	32	HAT 6
	Repeat	493 – 525	33	HAT 7
	Repeat	545 – 578	34	HAT 8
	Repeat	608 – 645	38	HAT 9
	Repeat	648 – 680	33	HAT 10
	Repeat	682 – 714	33	HAT 11
	Repeat	751 – 783	33	HAT 12
	Repeat	819 – 851	33	HAT 13

Sequences

	Sequence		Length	Mass (Da)
	P19735 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 3E87F8AF63EAAC41	FASTA	899	104,229
	10 20 30 40 50 60 MERPSFLDQE PPAGYVPGIG RGATGFSTKE KQVVSNDDKG RRIPKRYREN LNNHLQSQPK 70 80 90 100 110 120 DDEDDEAANV FKTLELKLAQ KKKKRANEKD DDNSVDSSNV KRQFADLKES LAAVTESEWM 130 140 150 160 170 180 DIPDATDFTR RNKRNRIQEQ LNRKTYAAPD SLIPGNVDLN KLTEEREKLL QSQIDENLAQ 190 200 210 220 230 240 LTKNASNPIQ VNKPNAATDA LSYLKDLEND RVNSLSDATL EDLQKMRTIL KSYRKADPTN 250 260 270 280 290 300 PQGWIASARL EEKARKFSVA KKIIENGCQE CPRSSDIWLE NIRLHESDVH YCKTLVATAI 310 320 330 340 350 360 NFNPTSPLLW FKAIDLESTT VNKYRVVRKA LQEIPRDEGL WKLAVSFEAD KAQVIKMLEK 370 380 390 400 410 420 ATQFIPQSMD LLTAYTNLQS YHNAKMTLNS FRKILPQEPE IWIISTLLEE RNNPDIPVDK 430 440 450 460 470 480 LVSLLKEGLL ELSKNGYKAT LSAWLKRAEA LNDAPNSNLT CQAIVYAILE WLRESGEYES 490 500 510 520 530 540 ELNNVDQILE KMPHSKVQIA VLKKLIQWDP CDTVLWSRLK MATESYHKIE ELLAFFQELL 550 560 570 580 590 600 FQTKNSDDIR ANMREKSPGL LMMYVSEYWK AQKGDTRQTL VLIDQIIDFA PHNLDLRFFK 610 620 630 640 650 660 IKLLGRSLQL DELRDFFQQT FSSLEDFKIS GTERLYYKYV NFLRYQDLNE EAIKFLNERC 670 680 690 700 710 720 LKSFPICHKF FLQLGQIYHS MGNIEMSRET YLSGTRLVPN CPLLWVSLSK IDEIDLKNPV 730 740 750 760 770 780 RARSILDRGL LKNPDDVLFY IAKIQMEIRL GNLDQAELLV TQALQKFPSN ALLWVEQIKL 790 800 810 820 830 840 FKHGNKSSLK KTIFQDALRR TQNDHRVLLE IGVSFYAEAQ YETSLKWLER ALKKCSRYGD 850 860 870 880 890 TWVWLFRTYA RLGKDTVDLY NMFDQCEPTY GPEWIAASKN VKMQYCTPRE ILLRLMNDK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The molecular characterization of PRP6 and PRP9 yeast genes reveals a new cysteine/histidine motif common to several splicing factors." Legrain P., Choulika A. EMBO J. 9:2775-2781(1990) [PubMed: 2118103] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / GRF88.
[2]	"Sequence and analysis of 24 kb on chromosome II of Saccharomyces cerevisiae." Aljinovic G., Pohl T.M. Yeast 11:475-479(1995) [PubMed: 7597852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X53465 Genomic DNA. Translation: CAA37559.1. Z35924 Genomic DNA. Translation: CAA84998.1. Z46260 Genomic DNA. Translation: CAA86398.1. BK006936 Genomic DNA. Translation: DAA07174.1.
PIR	S12319.
RefSeq	NP_009611.1. NM_001178403.1.
3D structure databases
ProteinModelPortal	P19735.
SMR	P19735. Positions 240-279, 636-770.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1290N.
IntAct	P19735. 66 interactions.
MINT	MINT-392841.
STRING	P19735.
Proteomic databases
PeptideAtlas	P19735.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR055C; YBR055C; YBR055C.
GeneID	852344.
KEGG	sce:YBR055C.
NMPDR	fig|4932.3.peg.306.
Organism-specific databases
CYGD	YBR055c.
SGD	S000000259. PRP6.
Phylogenomic databases
eggNOG	fuNOG04421.
GeneTree	EFGT00050000002823.
HOGENOM	HBG204135.
OMA	PQGWIAS.
OrthoDB	EOG4D82F8.
Gene expression databases
ArrayExpress	P19735.
Genevestigator	P19735.
GermOnline	YBR055C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003107. HAT. IPR010491. PRP1_N. IPR013026. TPR-contain. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. [Graphical view]
Gene3D	G3DSA:1.25.40.10. TPR-like_helical. 3 hits.
KO	K12855.
Pfam	PF06424. PRP1_N. 1 hit. [Graphical view]
SMART	SM00386. HAT. 7 hits. SM00028. TPR. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	971082.

Entry information

Entry name

PRP6_YEAST

Accession

Primary (citable) accession number: P19735
Secondary accession number(s): D6VQ54

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	December 14, 2011
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program