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P19735

- PRP6_YEAST

UniProt

P19735 - PRP6_YEAST

Protein

Pre-mRNA-splicing factor 6

Gene

PRP6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. mRNA splicing, via spliceosome Source: SGD

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29026-MONOMER.
    ReactomeiREACT_191540. mRNA Splicing - Minor Pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pre-mRNA-splicing factor 6
    Gene namesi
    Name:PRP6
    Synonyms:RNA6
    Ordered Locus Names:YBR055C
    ORF Names:YBR0508
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR055c.
    SGDiS000000259. PRP6.

    Subcellular locationi

    GO - Cellular componenti

    1. spliceosomal complex Source: UniProtKB-KW
    2. U4/U6 x U5 tri-snRNP complex Source: SGD

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 899899Pre-mRNA-splicing factor 6PRO_0000205756Add
    BLAST

    Proteomic databases

    MaxQBiP19735.
    PaxDbiP19735.
    PeptideAtlasiP19735.

    Expressioni

    Gene expression databases

    GenevestigatoriP19735.

    Interactioni

    Subunit structurei

    Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    DIB1Q068193EBI-227,EBI-166

    Protein-protein interaction databases

    BioGridi32756. 130 interactions.
    DIPiDIP-1290N.
    IntActiP19735. 24 interactions.
    MINTiMINT-392841.
    STRINGi4932.YBR055C.

    Structurei

    3D structure databases

    ProteinModelPortaliP19735.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati221 – 25333HAT 1Add
    BLAST
    Repeati255 – 28733HAT 2Add
    BLAST
    Repeati289 – 31830HAT 3Add
    BLAST
    Repeati319 – 35032HAT 4Add
    BLAST
    Repeati352 – 38130HAT 5Add
    BLAST
    Repeati383 – 41432HAT 6Add
    BLAST
    Repeati493 – 52533HAT 7Add
    BLAST
    Repeati545 – 57834HAT 8Add
    BLAST
    Repeati608 – 64538HAT 9Add
    BLAST
    Repeati648 – 68033HAT 10Add
    BLAST
    Repeati682 – 71433HAT 11Add
    BLAST
    Repeati751 – 78333HAT 12Add
    BLAST
    Repeati819 – 85133HAT 13Add
    BLAST

    Sequence similaritiesi

    Contains 13 HAT repeats.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0457.
    GeneTreeiENSGT00550000075016.
    HOGENOMiHOG000246836.
    KOiK12855.
    OMAiRQRFYAV.
    OrthoDBiEOG7GXPM5.

    Family and domain databases

    Gene3Di1.25.40.10. 2 hits.
    InterProiIPR003107. HAT.
    IPR010491. PRP1_N.
    IPR027108. Prp6/Prp1.
    IPR013026. TPR-contain_dom.
    IPR011990. TPR-like_helical.
    IPR019734. TPR_repeat.
    [Graphical view]
    PANTHERiPTHR11246:SF1. PTHR11246:SF1. 1 hit.
    PfamiPF06424. PRP1_N. 1 hit.
    [Graphical view]
    SMARTiSM00386. HAT. 7 hits.
    SM00028. TPR. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P19735-1 [UniParc]FASTAAdd to Basket

    « Hide

    MERPSFLDQE PPAGYVPGIG RGATGFSTKE KQVVSNDDKG RRIPKRYREN    50
    LNNHLQSQPK DDEDDEAANV FKTLELKLAQ KKKKRANEKD DDNSVDSSNV 100
    KRQFADLKES LAAVTESEWM DIPDATDFTR RNKRNRIQEQ LNRKTYAAPD 150
    SLIPGNVDLN KLTEEREKLL QSQIDENLAQ LTKNASNPIQ VNKPNAATDA 200
    LSYLKDLEND RVNSLSDATL EDLQKMRTIL KSYRKADPTN PQGWIASARL 250
    EEKARKFSVA KKIIENGCQE CPRSSDIWLE NIRLHESDVH YCKTLVATAI 300
    NFNPTSPLLW FKAIDLESTT VNKYRVVRKA LQEIPRDEGL WKLAVSFEAD 350
    KAQVIKMLEK ATQFIPQSMD LLTAYTNLQS YHNAKMTLNS FRKILPQEPE 400
    IWIISTLLEE RNNPDIPVDK LVSLLKEGLL ELSKNGYKAT LSAWLKRAEA 450
    LNDAPNSNLT CQAIVYAILE WLRESGEYES ELNNVDQILE KMPHSKVQIA 500
    VLKKLIQWDP CDTVLWSRLK MATESYHKIE ELLAFFQELL FQTKNSDDIR 550
    ANMREKSPGL LMMYVSEYWK AQKGDTRQTL VLIDQIIDFA PHNLDLRFFK 600
    IKLLGRSLQL DELRDFFQQT FSSLEDFKIS GTERLYYKYV NFLRYQDLNE 650
    EAIKFLNERC LKSFPICHKF FLQLGQIYHS MGNIEMSRET YLSGTRLVPN 700
    CPLLWVSLSK IDEIDLKNPV RARSILDRGL LKNPDDVLFY IAKIQMEIRL 750
    GNLDQAELLV TQALQKFPSN ALLWVEQIKL FKHGNKSSLK KTIFQDALRR 800
    TQNDHRVLLE IGVSFYAEAQ YETSLKWLER ALKKCSRYGD TWVWLFRTYA 850
    RLGKDTVDLY NMFDQCEPTY GPEWIAASKN VKMQYCTPRE ILLRLMNDK 899
    Length:899
    Mass (Da):104,229
    Last modified:February 1, 1991 - v1
    Checksum:i3E87F8AF63EAAC41
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53465 Genomic DNA. Translation: CAA37559.1.
    Z35924 Genomic DNA. Translation: CAA84998.1.
    Z46260 Genomic DNA. Translation: CAA86398.1.
    BK006936 Genomic DNA. Translation: DAA07174.1.
    PIRiS12319.
    RefSeqiNP_009611.1. NM_001178403.1.

    Genome annotation databases

    EnsemblFungiiYBR055C; YBR055C; YBR055C.
    GeneIDi852344.
    KEGGisce:YBR055C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53465 Genomic DNA. Translation: CAA37559.1 .
    Z35924 Genomic DNA. Translation: CAA84998.1 .
    Z46260 Genomic DNA. Translation: CAA86398.1 .
    BK006936 Genomic DNA. Translation: DAA07174.1 .
    PIRi S12319.
    RefSeqi NP_009611.1. NM_001178403.1.

    3D structure databases

    ProteinModelPortali P19735.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32756. 130 interactions.
    DIPi DIP-1290N.
    IntActi P19735. 24 interactions.
    MINTi MINT-392841.
    STRINGi 4932.YBR055C.

    Proteomic databases

    MaxQBi P19735.
    PaxDbi P19735.
    PeptideAtlasi P19735.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR055C ; YBR055C ; YBR055C .
    GeneIDi 852344.
    KEGGi sce:YBR055C.

    Organism-specific databases

    CYGDi YBR055c.
    SGDi S000000259. PRP6.

    Phylogenomic databases

    eggNOGi COG0457.
    GeneTreei ENSGT00550000075016.
    HOGENOMi HOG000246836.
    KOi K12855.
    OMAi RQRFYAV.
    OrthoDBi EOG7GXPM5.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29026-MONOMER.
    Reactomei REACT_191540. mRNA Splicing - Minor Pathway.

    Miscellaneous databases

    NextBioi 971082.
    PROi P19735.

    Gene expression databases

    Genevestigatori P19735.

    Family and domain databases

    Gene3Di 1.25.40.10. 2 hits.
    InterProi IPR003107. HAT.
    IPR010491. PRP1_N.
    IPR027108. Prp6/Prp1.
    IPR013026. TPR-contain_dom.
    IPR011990. TPR-like_helical.
    IPR019734. TPR_repeat.
    [Graphical view ]
    PANTHERi PTHR11246:SF1. PTHR11246:SF1. 1 hit.
    Pfami PF06424. PRP1_N. 1 hit.
    [Graphical view ]
    SMARTi SM00386. HAT. 7 hits.
    SM00028. TPR. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The molecular characterization of PRP6 and PRP9 yeast genes reveals a new cysteine/histidine motif common to several splicing factors."
      Legrain P., Choulika A.
      EMBO J. 9:2775-2781(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / GRF88.
    2. "Sequence and analysis of 24 kb on chromosome II of Saccharomyces cerevisiae."
      Aljinovic G., Pohl T.M.
      Yeast 11:475-479(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP."
      Gottschalk A., Neubauer G., Banroques J., Mann M., Luehrmann R., Fabrizio P.
      EMBO J. 18:4535-4548(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy."
      Hacker I., Sander B., Golas M.M., Wolf E., Karagoz E., Kastner B., Stark H., Fabrizio P., Luhrmann R.
      Nat. Struct. Mol. Biol. 15:1206-1212(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, ELECTRON MICROSCOPY.

    Entry informationi

    Entry nameiPRP6_YEAST
    AccessioniPrimary (citable) accession number: P19735
    Secondary accession number(s): D6VQ54
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2250 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3