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Protein

Pre-mRNA-splicing factor 6

Gene

PRP6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.

GO - Biological processi

  1. mRNA splicing, via spliceosome Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-29026-MONOMER.
ReactomeiREACT_294700. mRNA Splicing - Minor Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor 6
Gene namesi
Name:PRP6
Synonyms:RNA6
Ordered Locus Names:YBR055C
ORF Names:YBR0508
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome II

Organism-specific databases

CYGDiYBR055c.
EuPathDBiFungiDB:YBR055C.
SGDiS000000259. PRP6.

Subcellular locationi

GO - Cellular componenti

  1. spliceosomal complex Source: UniProtKB-KW
  2. U4/U6 x U5 tri-snRNP complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 899899Pre-mRNA-splicing factor 6PRO_0000205756Add
BLAST

Proteomic databases

MaxQBiP19735.
PaxDbiP19735.
PeptideAtlasiP19735.

Expressioni

Gene expression databases

GenevestigatoriP19735.

Interactioni

Subunit structurei

Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DIB1Q068193EBI-227,EBI-166

Protein-protein interaction databases

BioGridi32756. 132 interactions.
DIPiDIP-1290N.
IntActiP19735. 24 interactions.
MINTiMINT-392841.
STRINGi4932.YBR055C.

Structurei

3D structure databases

ProteinModelPortaliP19735.
SMRiP19735. Positions 495-538, 738-832.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati221 – 25333HAT 1Add
BLAST
Repeati255 – 28733HAT 2Add
BLAST
Repeati289 – 31830HAT 3Add
BLAST
Repeati319 – 35032HAT 4Add
BLAST
Repeati352 – 38130HAT 5Add
BLAST
Repeati383 – 41432HAT 6Add
BLAST
Repeati493 – 52533HAT 7Add
BLAST
Repeati545 – 57834HAT 8Add
BLAST
Repeati608 – 64538HAT 9Add
BLAST
Repeati648 – 68033HAT 10Add
BLAST
Repeati682 – 71433HAT 11Add
BLAST
Repeati751 – 78333HAT 12Add
BLAST
Repeati819 – 85133HAT 13Add
BLAST

Sequence similaritiesi

Contains 13 HAT repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0457.
GeneTreeiENSGT00550000075016.
HOGENOMiHOG000246836.
InParanoidiP19735.
KOiK12855.
OMAiNCAKEGA.
OrthoDBiEOG7GXPM5.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR003107. HAT.
IPR010491. PRP1_N.
IPR027108. Prp6/Prp1/STA1.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR11246:SF1. PTHR11246:SF1. 1 hit.
PfamiPF06424. PRP1_N. 1 hit.
[Graphical view]
SMARTiSM00386. HAT. 7 hits.
SM00028. TPR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19735-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERPSFLDQE PPAGYVPGIG RGATGFSTKE KQVVSNDDKG RRIPKRYREN
60 70 80 90 100
LNNHLQSQPK DDEDDEAANV FKTLELKLAQ KKKKRANEKD DDNSVDSSNV
110 120 130 140 150
KRQFADLKES LAAVTESEWM DIPDATDFTR RNKRNRIQEQ LNRKTYAAPD
160 170 180 190 200
SLIPGNVDLN KLTEEREKLL QSQIDENLAQ LTKNASNPIQ VNKPNAATDA
210 220 230 240 250
LSYLKDLEND RVNSLSDATL EDLQKMRTIL KSYRKADPTN PQGWIASARL
260 270 280 290 300
EEKARKFSVA KKIIENGCQE CPRSSDIWLE NIRLHESDVH YCKTLVATAI
310 320 330 340 350
NFNPTSPLLW FKAIDLESTT VNKYRVVRKA LQEIPRDEGL WKLAVSFEAD
360 370 380 390 400
KAQVIKMLEK ATQFIPQSMD LLTAYTNLQS YHNAKMTLNS FRKILPQEPE
410 420 430 440 450
IWIISTLLEE RNNPDIPVDK LVSLLKEGLL ELSKNGYKAT LSAWLKRAEA
460 470 480 490 500
LNDAPNSNLT CQAIVYAILE WLRESGEYES ELNNVDQILE KMPHSKVQIA
510 520 530 540 550
VLKKLIQWDP CDTVLWSRLK MATESYHKIE ELLAFFQELL FQTKNSDDIR
560 570 580 590 600
ANMREKSPGL LMMYVSEYWK AQKGDTRQTL VLIDQIIDFA PHNLDLRFFK
610 620 630 640 650
IKLLGRSLQL DELRDFFQQT FSSLEDFKIS GTERLYYKYV NFLRYQDLNE
660 670 680 690 700
EAIKFLNERC LKSFPICHKF FLQLGQIYHS MGNIEMSRET YLSGTRLVPN
710 720 730 740 750
CPLLWVSLSK IDEIDLKNPV RARSILDRGL LKNPDDVLFY IAKIQMEIRL
760 770 780 790 800
GNLDQAELLV TQALQKFPSN ALLWVEQIKL FKHGNKSSLK KTIFQDALRR
810 820 830 840 850
TQNDHRVLLE IGVSFYAEAQ YETSLKWLER ALKKCSRYGD TWVWLFRTYA
860 870 880 890
RLGKDTVDLY NMFDQCEPTY GPEWIAASKN VKMQYCTPRE ILLRLMNDK
Length:899
Mass (Da):104,229
Last modified:February 1, 1991 - v1
Checksum:i3E87F8AF63EAAC41
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53465 Genomic DNA. Translation: CAA37559.1.
Z35924 Genomic DNA. Translation: CAA84998.1.
Z46260 Genomic DNA. Translation: CAA86398.1.
BK006936 Genomic DNA. Translation: DAA07174.1.
PIRiS12319.
RefSeqiNP_009611.1. NM_001178403.1.

Genome annotation databases

EnsemblFungiiYBR055C; YBR055C; YBR055C.
GeneIDi852344.
KEGGisce:YBR055C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53465 Genomic DNA. Translation: CAA37559.1.
Z35924 Genomic DNA. Translation: CAA84998.1.
Z46260 Genomic DNA. Translation: CAA86398.1.
BK006936 Genomic DNA. Translation: DAA07174.1.
PIRiS12319.
RefSeqiNP_009611.1. NM_001178403.1.

3D structure databases

ProteinModelPortaliP19735.
SMRiP19735. Positions 495-538, 738-832.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32756. 132 interactions.
DIPiDIP-1290N.
IntActiP19735. 24 interactions.
MINTiMINT-392841.
STRINGi4932.YBR055C.

Proteomic databases

MaxQBiP19735.
PaxDbiP19735.
PeptideAtlasiP19735.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR055C; YBR055C; YBR055C.
GeneIDi852344.
KEGGisce:YBR055C.

Organism-specific databases

CYGDiYBR055c.
EuPathDBiFungiDB:YBR055C.
SGDiS000000259. PRP6.

Phylogenomic databases

eggNOGiCOG0457.
GeneTreeiENSGT00550000075016.
HOGENOMiHOG000246836.
InParanoidiP19735.
KOiK12855.
OMAiNCAKEGA.
OrthoDBiEOG7GXPM5.

Enzyme and pathway databases

BioCyciYEAST:G3O-29026-MONOMER.
ReactomeiREACT_294700. mRNA Splicing - Minor Pathway.

Miscellaneous databases

NextBioi971082.
PROiP19735.

Gene expression databases

GenevestigatoriP19735.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR003107. HAT.
IPR010491. PRP1_N.
IPR027108. Prp6/Prp1/STA1.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR11246:SF1. PTHR11246:SF1. 1 hit.
PfamiPF06424. PRP1_N. 1 hit.
[Graphical view]
SMARTiSM00386. HAT. 7 hits.
SM00028. TPR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The molecular characterization of PRP6 and PRP9 yeast genes reveals a new cysteine/histidine motif common to several splicing factors."
    Legrain P., Choulika A.
    EMBO J. 9:2775-2781(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / GRF88.
  2. "Sequence and analysis of 24 kb on chromosome II of Saccharomyces cerevisiae."
    Aljinovic G., Pohl T.M.
    Yeast 11:475-479(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP."
    Gottschalk A., Neubauer G., Banroques J., Mann M., Luehrmann R., Fabrizio P.
    EMBO J. 18:4535-4548(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy."
    Hacker I., Sander B., Golas M.M., Wolf E., Karagoz E., Kastner B., Stark H., Fabrizio P., Luhrmann R.
    Nat. Struct. Mol. Biol. 15:1206-1212(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, ELECTRON MICROSCOPY.

Entry informationi

Entry nameiPRP6_YEAST
AccessioniPrimary (citable) accession number: P19735
Secondary accession number(s): D6VQ54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: April 29, 2015
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2250 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.