P19733 (DMPO_PSEUF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenol hydroxylase P4 protein
EC=1.14.13.7

Alternative name(s):
Phenol 2-monooxygenase P4 component

Gene names

Name:	dmpO
Synonyms:	pheA5

Encoded on

Plasmid pVI150

Organism

Pseudomonas sp. (strain CF600)

Taxonomic identifier

79676 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria

Protein attributes

Sequence length	119 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Catabolizes phenol, and some of its methylated derivatives. P4 is required for growth on phenol, and for in vitro phenol hydroxylase activity.
Catalytic activity	Phenol + NADPH + O2 = catechol + NADP+ + H2O.
Cofactor	FAD. Fe2+.
Pathway	Aromatic compound metabolism; phenol degradation.
Subunit structure	The multicomponent enzyme phenol hydroxylase is formed by P0, P1, P2, P3, P4 and P5 polypeptides.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	FAD Flavoprotein Iron NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	phenol 2-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 119	119	Phenol hydroxylase P4 protein		PRO_0000079946

Sequences

	Sequence		Length	Mass (Da)
	P19733 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: AE0151A918638C49	FASTA	119	13,207
	10 20 30 40 50 60 MTVNSIGEYT ATPRDVQANF NGMQLLYLYW EEHLMYCSAL AFLVAPGMPF AEFLEQVLKP 70 80 90 100 110 AIHAHPDSAK IDFSQALWQL NDQPFTPDYA ASLEANGIDH KSMLRLNTPG LNGIQGSCS

References

[1]	"Complete nucleotide sequence and polypeptide analysis of multicomponent phenol hydroxylase from Pseudomonas sp. strain CF600." Nordlund I., Powlowski J., Shingler V. J. Bacteriol. 172:6826-6833(1990) [PubMed: 2254258] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Takeo M., Maeda Y., Okada H., Miyama K., Mori K., Ike M., Fujita M. Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BH.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M60276 Genomic DNA. Translation: AAA25943.1. D28864 Genomic DNA. Translation: BAA06018.1.
3D structure databases
ProteinModelPortal	P19733.
SMR	P19733. Positions 2-118.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-12798.
Family and domain databases
InterPro	IPR006756. Phenol_hydroxylase. [Graphical view]
Pfam	PF04663. Phenol_monoox. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DMPO_PSEUF

Accession

Primary (citable) accession number: P19733

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	June 28, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program