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P19730 (DMPL_PSEUF) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phenol hydroxylase P1 protein

EC=1.14.13.7
Alternative name(s):
Phenol 2-monooxygenase P1 component
Gene names
Name:dmpL
Synonyms:pheA2
Encoded onPlasmid pVI150
OrganismPseudomonas sp. (strain CF600)
Taxonomic identifier79676 [NCBI]
Taxonomic lineageBacteriaProteobacteria

Protein attributes

Sequence length331 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Catabolizes phenol, and some of its methylated derivatives. P1 is required for growth on phenol, and for in vitro phenol hydroxylase activity.

Catalytic activity

Phenol + NADPH + O2 = catechol + NADP+ + H2O.

Cofactor

FAD.

Fe2+.

Pathway

Aromatic compound metabolism; phenol degradation.

Subunit structure

The multicomponent enzyme phenol hydroxylase is formed by P0, P1, P2, P3, P4 and P5 polypeptides.

Ontologies

Keywords
   Biological processAromatic hydrocarbons catabolism
   LigandFAD
Flavoprotein
Iron
NADP
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termPlasmid
Gene Ontology (GO)
   Biological_processaerobic phenol-containing compound catabolic process

Inferred from direct assay PubMed 225425. Source: UniProtKB

   Cellular_componentextrachromosomal circular DNA

Inferred from direct assay Ref.1. Source: UniProtKB

   Molecular_functionphenol 2-monooxygenase activity

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 331331Phenol hydroxylase P1 protein
PRO_0000079942

Sequences

Sequence LengthMass (Da)Tools
P19730 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 8042F5723BE3A5E8

FASTA33138,208
        10         20         30         40         50         60 
MSVEIKTNTV DPIRQTYGNL QRRFGDKPAS RYQEASYDIE AVTNFHYRPL WDPQHELHDP 

        70         80         90        100        110        120 
TRTAIRMTDW HKVTDPRQFY YGAYVQTRAR MQEATEHAYG FCEKRELLSR LPAELQAKLL 

       130        140        150        160        170        180 
RCLVPLRHAE LGANMNNSSI AGDSIAATVT QMHIYQAMDR LGMGQYLSRI GLLLDGGTGE 

       190        200        210        220        230        240 
ALDQAKAYWL DDPIWQGLRR YVEDSFVIRD WFELGLAQNL VLDGLLQPLM YQRFDQWLTE 

       250        260        270        280        290        300 
NGGSDVAMLT EFMRDWYGES TRWVDAMFKT VLAENDANRE QVQAWLEVWE PRAYEALLPL 

       310        320        330 
AEEATGIAAL DEVRSAFATR LQKIGLKSRE E 

« Hide

References

[1]"Complete nucleotide sequence and polypeptide analysis of multicomponent phenol hydroxylase from Pseudomonas sp. strain CF600."
Nordlund I., Powlowski J., Shingler V.
J. Bacteriol. 172:6826-6833(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Takeo M., Maeda Y., Okada H., Miyama K., Mori K., Ike M., Fujita M.
Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BH.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M60276 Genomic DNA. Translation: AAA25940.1.
D28864 Genomic DNA. Translation: BAA06015.1.

3D structure databases

ProteinModelPortalP19730.
SMRP19730. Positions 4-329.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-12795.
UniPathwayUPA00728.

Family and domain databases

Gene3D1.10.620.20. 1 hit.
InterProIPR009078. Ferritin-like_SF.
IPR012078. MP_mOase_hydro.
IPR003430. Phenol_Hydrox.
IPR012348. RNR-rel.
[Graphical view]
PfamPF02332. Phenol_Hydrox. 1 hit.
[Graphical view]
PIRSFPIRSF000040. MMOH_comp. 1 hit.
SUPFAMSSF47240. SSF47240. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDMPL_PSEUF
AccessionPrimary (citable) accession number: P19730
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: December 11, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways