Reviewed, UniProtKB/Swiss-Prot P19730 (DMPL_PSEUF)

Names and origin

Protein names

Recommended name:
    Phenol hydroxylase P1 protein
    EC=1.14.13.7
Alternative name(s):
    Phenol 2-monooxygenase P1 component

Gene names

Name:	dmpL
Synonyms:	pheA2

Encoded on

Plasmid pVI150

Organism

Pseudomonas sp. (strain CF600)

Taxonomic identifier

79676 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Predicted.

General annotation (Comments)

Function	Catabolizes phenol, and some of its methylated derivatives. P1 is required for growth on phenol, and for in vitro phenol hydroxylase activity.
Catalytic activity	Phenol + NADPH + O2 = catechol + NADP+ + H2O.
Cofactor	FAD. Fe2+.
Pathway	Aromatic compound metabolism; phenol degradation.
Subunit structure	The multicomponent enzyme phenol hydroxylase is formed by P0, P1, P2, P3, P4 and P5 polypeptides.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	FAD Flavoprotein Iron NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	aerobic phenol catabolic process Inferred from direct assay. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrachromosomal circular DNA Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenol 2-monooxygenase activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	Phenol hydroxylase P1 protein		PRO_0000079942

Sequences

	Sequence		Length	Mass (Da)
	P19730-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 8042F5723BE3A5E8	FASTA	331	38,208
	10 20 30 40 50 60 MSVEIKTNTV DPIRQTYGNL QRRFGDKPAS RYQEASYDIE AVTNFHYRPL WDPQHELHDP 70 80 90 100 110 120 TRTAIRMTDW HKVTDPRQFY YGAYVQTRAR MQEATEHAYG FCEKRELLSR LPAELQAKLL 130 140 150 160 170 180 RCLVPLRHAE LGANMNNSSI AGDSIAATVT QMHIYQAMDR LGMGQYLSRI GLLLDGGTGE 190 200 210 220 230 240 ALDQAKAYWL DDPIWQGLRR YVEDSFVIRD WFELGLAQNL VLDGLLQPLM YQRFDQWLTE 250 260 270 280 290 300 NGGSDVAMLT EFMRDWYGES TRWVDAMFKT VLAENDANRE QVQAWLEVWE PRAYEALLPL 310 320 330 AEEATGIAAL DEVRSAFATR LQKIGLKSRE E

References

[1]	"Complete nucleotide sequence and polypeptide analysis of multicomponent phenol hydroxylase from Pseudomonas sp. strain CF600." Nordlund I., Powlowski J., Shingler V. J. Bacteriol. 172:6826-6833(1990) [PubMed: 2254258] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Takeo M., Maeda Y., Okada H., Miyama K., Mori K., Ike M., Fujita M. Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BH.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M60276 Genomic DNA. Translation: AAA25940.1. D28864 Genomic DNA. Translation: BAA06015.1.
3D structure databases
SMR	P19730. Positions 4-329.
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-12795.
Family and domain databases
InterPro	IPR009078. Ferritin/RR-like. IPR012078. MP_mOase_hydro. IPR003430. Phenol_Hydrox. IPR012348. Ribncl_red_rel. [Graphical view]
Gene3D	G3DSA:1.10.620.20. Ribncl_red_rel. 1 hit.
Pfam	PF02332. Phenol_Hydrox. 1 hit. [Graphical view]
PIRSF	PIRSF000040. MMOH_comp. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DMPL_PSEUF

Accession

Primary (citable) accession number: P19730

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	January 19, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)